期刊
BIOMATERIALS
卷 33, 期 9, 页码 2754-2761出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.12.015
关键词
DNAzyme; Drug delivery; Magnetic nanoparticle; Virus
资金
- National Research Foundation of Korea (NRF)
- Korean government (the Ministry of Education, Science and Technology, MEST) [313-2008-2-C00538, 2008-0062074, 2011-0020322, 2011-0017356]
- NRF
- MEST [2008-2004457]
- MEST, Korea
- National Research Foundation of Korea [2008-2004457, 2008-0062074, 2011-0017356, 313-2008-2-C00538, 2011-0020322] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
DNAzyme is an attractive therapeutic oligonucleotide which enables cleavage of mRNA in a sequence-specific manner and thus, silencing target gene. A particularly important challenge in achieving the successful down-regulation of gene expression is to efficiently deliver DNAzymes to disease sites and cells. Here, we report the nanoparticle-assisted functional delivery of therapeutic DNAzyme for the treatment of hepatitis C by inducing knockdown of hepatitis C virus (HCV) gene, NS3. HCV NS3 gene encodes helicase and protease which are essential for the virus replication. The nanocomplex showed efficient NS3 knockdown while not evoking undesired immune responses or notable cytotoxicity. We also demonstrated the DNAzyme conjugated nanoparticle system could be applicable in vivo by showing the accumulation of the nanoparticles in liver, and more specifically, in hepatocytes. We believe that the present work is a successful demonstration of effective, functional, non-immunostimulatory DNAzyme delivery system based on inorganic nanoparticles with high potential for further therapeutic application of DNAzyme in the treatment of hepatitis C. (C) 2011 Elsevier Ltd. All rights reserved.
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