Article
Chemistry, Medicinal
Olimpia Ortiz-Arrabal, Ainhoa Irastorza-Lorenzo, Fernando Campos, Miguel Angel Martin-Piedra, Victor Carriel, Ingrid Garzon, Paula Avila-Fernandez, Maria Jose de Frutos, Emilio Esteban, Javier Fernandez, Agustin Janer, Antonio Campos, Jesus Chato-Astrain, Miguel Alaminos
Summary: Marine-origin polysaccharides, especially agarose, have been extensively studied as scaffolds for tissue engineering. We have developed new fibrin-agarose (FA) biomaterials using different types and concentrations of agaroses, which showed high biocompatibility and varied biomechanical properties. In vivo experiments demonstrated the biocompatibility of FA tissues and histological analysis indicated a pro-regenerative process with M2-type CD206-positive macrophages. These findings confirm the clinical potential of FA biomaterials for tissue engineering applications, allowing for the selection of specific agarose types and concentrations to meet desired biomechanical properties and in vivo reabsorption times.
Article
Biotechnology & Applied Microbiology
Janine Tomasch, Babette Maleiner, Philipp Heher, Manuel Rufin, Orestis G. Andriotis, Philipp J. Thurner, Heinz Redl, Christiane Fuchs, Andreas H. Teuschl-Woller
Summary: This study aimed to investigate the influence of apparent elastic properties of fibrin scaffolds on myoblasts and evaluate if those effects differ between murine and human cells. The results showed that the elastic modulus of fibrin hydrogels had different effects on myoblast proliferation and differentiation, with a more pronounced impact on human cells in a 3D environment compared to 2D. Furthermore, the differentiation potential of human myoblasts was significantly impaired when incorporated into 3D gels. These findings provide insights into the cellular behavior and myogenic outcome of skeletal muscle tissue engineering approaches, highlighting the need for adjusting 3D culture parameters when working with human cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Sarah M. Somers, Warren L. Grayson
Summary: Bioreactor systems play a crucial role in tissue engineering by guiding cell differentiation and maturation. By combining with electrospun fibrin microfibers, the systems can be effectively applied in muscle tissue engineering. The system is also able to test the mechanical properties of hydrogel-based scaffolds and apply tensile strain for relevant experiments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Review
Cell & Tissue Engineering
Souzan Salemi, Jenny A. Prange, Valentin Baumgartner, Deana Mohr-Haralampieva, Daniel Eberli
Summary: Tissue engineering has seen significant advancements in recent years, particularly in the application of stem cells for skeletal and smooth muscle cell engineering. The use of adult stem cells for muscle engineering shows promise, but still faces challenges in selecting the appropriate cell type and controlling differentiation and proliferation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Polymer Science
Catherine G. Y. Ngan, Anita Quigley, Richard J. Williams, Cathal D. O'Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, Penny McKelvie, Gordon G. Wallace, Peter F. M. Choong, Rob M. I. Kapsa
Summary: This study developed a biofabrication technique to engineer muscle for research and clinical applications. It demonstrated myoblast migration through a bioprinted GelMA scaffold, showing cells spontaneously forming fibers on the material surface, leading to advanced maturation of muscle tissue. This methodology has wide applications for in vitro and in vivo neuromuscular function and disease modeling.
Article
Engineering, Biomedical
Marie-Claire Regenberg, Mathias Wilhelmi, Andres Hilfiker, Axel Haverich, Thomas Aper
Summary: This study conducted a structural analysis of compacted fibrin tubes and found a strong correlation between fibrin's structure and its biomechanical strength. It also demonstrated that fibrin compaction can be enhanced through a dehydration process, leading to a significant increase in biomechanical strength. This method, combined with an adequate imaging technique, has potential as a powerful tool for quality assurance in the development of fibrin-based vascular grafts.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Qianqian Wang, Jiafang Wang, Elmira Tokhtaeva, Zhen Li, Martin G. Martin, Xuefeng B. Ling, James C. Y. Dunn
Summary: The study presents the development of a living intestinal muscle patch capable of generating different motility patterns and multiple digesta manipulations. It is shown that the successful generation of the contracting muscle patch requires both biological factors and cues from the scaffold. The findings highlight the importance of combining biomolecular and biomaterial approaches for tissue engineering, providing a more physiological model for studying gastrointestinal motility and drug discovery.
ADVANCED MATERIALS
(2023)
Review
Engineering, Biomedical
Paula Pleguezuelos-Beltran, Patricia Galvez-Martin, Daniel Nieto-Garcia, Juan Antonio Marchal, Elena Lopez-Ruiz
Summary: Skin sprays, an innovative technique for treating acute and chronic wounds, offer great potential for delivering cells and hydrogels. Compared to conventional treatments, they have advantages such as ease of application, the ability to treat large wound areas, and uniform distribution of materials. This article reviews the latest advances in skin spray technology, including investigational and commercially available products, and discusses regulatory pathways and clinical trials for different skin diseases.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Hung-Jin Huang, Yu-Hsuan Lee, Yung-Ho Hsu, Chia-Te Liao, Yuh-Feng Lin, Hui-Wen Chiu
Summary: Millions of experimental animals are widely used in evaluating the toxicological or biological effects of manufactured nanomaterials in medical technology, but the principle of the 3Rs is applied to ensure more ethical animal research. Alternative methods, such as in vitro and in silico approaches, have been employed to replace or reduce the use of experimental animals and have been implemented in many countries for scientific purposes. These non-animal approaches, including cell culture testing, tissue engineering, molecular docking, molecular dynamics simulations, and QSAR modeling, have the potential to help minimize the use of experimental animals for nanomaterial toxicity assessments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Rowoon Park, Jung Won Yoon, Jin-Ho Lee, Suck Won Hong, Jae Ho Kim
Summary: Researchers have developed an ultrathin cell-culture platform with potential-responsive nanoscale biointerfaces for monitoring mesenchymal stem cells (MSCs). The platform allows for highly ordered organization and spontaneous differentiation of MSCs, providing a robust strategy for directing stem cells and differentiation.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Ravikumar Vaghela, Andreas Arkudas, Daniel Gage, Carolin Korner, Stephan von Hoersten, Sahar Salehi, Raymund E. Horch, Maximilian Hessenauer
Summary: The AV loop model is a key technique in tissue engineering that addresses the problem of vascular support. This study used a novel intravital microscopy approach to observe the process of vascularization in hydrogels. GelMA proved to be a better matrix compared to fibrin in terms of microvessel formation and network maturation. This study provides a new tool for understanding important events in tissue regeneration.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Chemistry, Multidisciplinary
Sadegh Davoudi, Bin Xu, Erik Jacques, Jose L. Cadavid, Matthew McFee, Chih-Ying Chin, Amir Meysami, Majid Ebrahimi, Mohsen A. Bakooshli, Kayee Tung, Henry Ahn, Howard J. Ginsberg, Alison P. McGuigan, Penney M. Gilbert
Summary: Stem cell-mediated endogenous repair is often evaluated through animal studies, but this study describes an in vitro assay that mimics important early stages of in vivo skeletal muscle repair. By integrating a semi-automated image analysis pipeline, high-content data analysis of muscle fiber content and morphology is enabled. The study found that the spatiotemporal dynamics of in vitro repair closely matched those observed in vivo only when both stem cells and injury were present. The engineered tissue's imaging geometry allowed for exploration of cellular mechanisms driving the muscle stem cell response to the regenerative template.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Natsumi Ueda, Shiho Sawada, Fumiya Yuasa, Karen Kato, Koji Nagahama
Summary: This study reports an injectable covalent stem cell-combining biohybrid material for in vivo tissue engineering, which can achieve controlled differentiation and integration with host tissues. By incorporating specific modified stem cells and alginate polymers in a cross-linking agent, the mechanical properties of the material can be adjusted. Experimental results in a mouse muscle defect model demonstrate that this covalent material treatment can effectively promote tissue regeneration with better outcomes compared to control materials.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Ako Ishihara, Jun Ishihara, Elyse A. Watkins, Andrew C. Tremain, Mindy Nguyen, Ani Solanki, Kiyomitsu Katsumata, Aslan Mansurov, Erica Budina, Aaron T. Alpar, Peyman Hosseinchi, Lea Maillat, Joseph W. Reda, Takahiro Kageyama, Melody A. Swartz, Eiji Yuba, Jeffrey A. Hubbell
Summary: The SA-IL-4 fusion protein effectively prevents disease development in mice with EAE by reducing spinal cord infiltration of immune cells, decreasing integrin expression in antigen-specific CD4(+)T cells, increasing the number of granulocyte-like myeloid-derived suppressor cells, and decreasing the number of pathogenic T helper 17 cells.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Immunology
Martina Damo, D. Scott Wilson, Elyse A. Watkins, Jeffrey A. Hubbell
Summary: Hepatocytes play a crucial role in inducing immunologic tolerance in the liver through cross-presentation of extracellular antigens. This study demonstrates that hepatocytes are capable of efficiently inducing cross-tolerance of specific T cells, providing evidence for their potential as valuable candidates for targeted tolerogenic treatments in vivo.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Maria Stella Sasso, Nikolaos Mitrousis, Yue Wang, Priscilla S. Briquez, Sylvie Hauert, Jun Ishihara, Jeffrey A. Hubbell, Melody A. Swartz
Summary: The induction of lymphatic growth in melanoma has been linked to poor prognosis, but recent research has shown that it can enhance cancer immunotherapy and boost T cell immunity. Developing a translational approach, the study found that inducing lymphangiogenesis in cancer vaccines can lead to stronger T cell activation and higher frequencies of antigen-specific T cells, ultimately providing effective tumor control and long-term immunological memory in mouse melanoma models.
Article
Multidisciplinary Sciences
Priscilla S. Briquez, Hsiu-Ming Tsai, Elyse A. Watkins, Jeffrey A. Hubbell
Summary: A new translational protein engineering-based approach has been developed to reduce the required dosage of rhBMP-2 in clinical spinal fusion, increasing safety when delivered in a collagen sponge. This method has shown improved efficacy at low doses of rhBMP-2 in two mouse models of bone regeneration and may have applications in various regenerative medicine fields.
Article
Engineering, Biomedical
Laura T. Gray, Michal M. Raczy, Priscilla S. Briquez, Tiffany M. Marchell, Aaron T. Alpar, Rachel P. Wallace, Lisa R. Volpatti, Maria Stella Sasso, Shijie Cao, Mindy Nguyen, Aslan Mansurov, Erica Budina, Elyse A. Watkins, Ani Solanki, Nikolaos Mitrousis, Joseph W. Reda, Shann S. Yu, Andrew C. Tremain, Ruyi Wang, Vlad Nicolaescu, Kevin Furlong, Steve Dvorkin, Balaji Manicassamy, Glenn Randall, D. Scott Wilson, Marcin Kwissa, Melody A. Swartz, Jeffrey A. Hubbell
Summary: Spike-p(Man-TLR7) vaccination induces robust antigen-specific cellular and humoral responses in mice, generating high and long-lasting titers of antibodies with neutralizing abilities surpassing convalescent human serum. The vaccine also enhances germinal center B cells, antigen-specific antibody secreting cells, activated T follicular helper cells, and polyfunctional Th1-cytokine producing CD4(+) and CD8(+) T cells.
Article
Cell & Tissue Engineering
Michael J. V. White, Priscilla S. Briquez, David A. V. White, Jeffrey A. Hubbell
Summary: Chronic non-healing wounds caused by diabetes result from damage to the extracellular matrix (ECM), and engineered growth factors can effectively improve wound healing. In a mouse model, the combination of three growth factors enhances efficacy, and changes in the wound cellular environment are predictive of healing success.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Aslan Mansurov, Peyman Hosseinchi, Kevin Chang, Abigail L. Lauterbach, Laura T. Gray, Aaron T. Alpar, Erica Budina, Anna J. Slezak, Seounghun Kang, Shijie Cao, Ani Solanki, Suzana Gomes, John-Michael Williford, Melody A. Swartz, Juan L. Mendoza, Jun Ishihara, Jeffrey A. Hubbell
Summary: Fusing a domain of the IL-12 receptor to IL-12 via a linker cleavable by tumour-associated proteases can restrict the pro-inflammatory effects of IL-12 to tumour sites, eliminating systemic immune-related adverse events and triggering potent therapeutic effects in mice bearing immunologically cold tumours.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Polymer Science
Adam M. Weiss, Samir Hossainy, Stuart J. Rowan, Jeffrey A. Hubbell, Aaron P. Esser-Kahn
Summary: This study discusses the application of immunostimulatory polymers in therapeutics and their ability to activate the immune system, as well as the design and delivery methods involved.
Article
Cell & Tissue Engineering
Jialu Liu, Ani Solanki, Michael J. V. White, Jeffrey A. Hubbell, Priscilla S. Briquez
Summary: This study explores the potential of using alpha 2-antiplasmin (α 2PI), a human physiological fibrinolysis inhibitor, as a substitute for aprotinin. The researchers found that α 2PI can prolong the durability of fibrin biomaterials, enhance delivery of growth factors for wound healing, and effectively prevent blood loss as a hemostatic agent.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Engineering, Biomedical
Maximilian G. Burger, Andrea Grosso, Priscilla S. Briquez, Gordian M. E. Born, Alexander Lunger, Flavio Schrenk, Atanas Todorov, Veronica Sacchi, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: In regenerative medicine, rapid vascularization of clinical-size bone grafts is a challenge that has not been solved. This study investigates the distribution of VEGF protein on matrices to achieve efficient coupling of angiogenesis and bone formation, providing a clinically applicable strategy for engineering vascularized bone.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Ruyi Wang, Shijie Cao, Mohamed Elfatih H. Bashir, Lauren A. Hesser, Yanlin Su, Sung Min Choi Hong, Andrew Thompson, Elliot Culleen, Matthew Sabados, Nicholas P. Dylla, Evelyn Campbell, Riyue Bao, Eric B. Nonnecke, Charles L. Bevins, D. Scott Wilson, Jeffrey A. Hubbell, Cathryn R. Nagler
Summary: The depletion of beneficial taxa in the gut, including butyrate-producing Clostridia, has led to the increase in food allergies and inflammatory bowel disease. By delivering butyrate-releasing micelles to the intestinal tract, barrier-protective responses can be restored, reducing disease severity in mouse models of colitis and peanut allergy. This approach can potentially treat allergic and inflammatory diseases by restoring microbial and mucosal homeostasis.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Andrea Grosso, Alexander Lunger, Maximilian G. Burger, Priscilla S. Briquez, Francesca Mai, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: Vascular endothelial growth factor-A (VEGF) has a physiological role in regulating both angiogenesis and osteogenesis, but its application in bone tissue engineering has produced conflicting outcomes. This study investigates the impact of VEGF dose on the coordination between angiogenesis and osteogenesis. The findings suggest that a specific range of VEGF dose is required to stimulate both vascular invasion and osteogenic commitment, resulting in improved bone formation. The study also reveals that VEGF dose regulates Notch1 activation and induces a pro-osteogenic endothelial phenotype. Fine-tuning of VEGF dose is crucial for achieving the physiological coupling of accelerated vascular invasion and enhanced bone formation.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Engineering, Biomedical
Abigail L. Lauterbach, Anna J. Slezak, Ruyi Wang, Shijie Cao, Michal M. Raczy, Elyse A. Watkins, Carlos J. Medina Jimenez, Jeffrey A. Hubbell
Summary: Butyrate, an important bacterial metabolite, has a complex role in regulating immunity and maintaining epithelial barriers. However, its clinical translation is hindered by poor bioavailability, strong odor, and high dosage requirements. A novel polymeric delivery platform has been developed for controlled release of butyrate, which also has potential for other therapeutically relevant metabolites. This platform shows efficacy in the treatment of non-healing diabetic wounds by modulating immune cell activation and altering the soluble signals in the wound microenvironment, addressing a significant clinical need.
ADVANCED HEALTHCARE MATERIALS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Adelin Rouchon, Priscilla S. Briquez, Dirk J. Schaefer, Jeffrey A. Hubbell, Nunzia Di Maggio, Andrea Banfi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Andrea Banfi, Andrea Grosso, Priscilla S. Briquez, Dirk J. Schaefer, Jeffrey A. Hubbell, Nunzia Di Maggio
TISSUE ENGINEERING PART A
(2022)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.