期刊
BIOMATERIALS
卷 32, 期 28, 页码 6860-6874出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.05.079
关键词
PLGA nanoparticles; CoQ10; Oxidative stress; Hepatoprotective activity; Anti-inflammatory activity; Mitochondria and lysosomes localization
资金
- Department of Science & Technology (DST), Government of India, New Delhi, India
The present investigation consists in the development and characterization of CoQ10 loaded PLGA nanoparticles (CoQ10-NPs, size < 100 nm) by a scalable emulsion-diffusion-evaporation method. Thermal and crystallinity analysis collectively corroborated that CoQ10 was entrapped into the NPs in amorphous form. The lyophilized CoQ10-NPs were found to be stable for a period of 6 months (at room temperature). In vitro cell culture studies indicated that CoQ10-NPs significantly quenched ROS with nearly 10 fold higher efficacy than free CoQ10. Further, positively charged CoQ10-NPs were localized in two major sources of ROS generation: mitochondria and lysosomes. CoQ10-NP5 showed improved oral bioavailability (4.28 times) as compared to free CoQ10. Finally remarkably higher hepatoprotective and anti-inflammatory activity of CoQ10-NPs as compared to free CoQ10 was observed due to mitigation of deleterious effects associated with the generation of free radicals. As elucidated by live noninvasive animal imaging, the higher anti-inflammatory activity of CoQ10-NP5 can be attributed to significant accumulation of these NPs in the inflamed tissues. (C) 2011 Elsevier Ltd. All rights reserved.
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