期刊
BIOMATERIALS
卷 32, 期 8, 页码 2149-2159出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.11.052
关键词
Silk; Fibroin; 3D; Cell culture; Tumour
资金
- Department of Biotechnology
- Indo-Australia Biotechnology fund
- Department of Science and Technology
- Government of India Indo-US Science and Technology Forum (IUSSTF), New Delhi
- Australian Prostate Cancer Foundation
3D in vitro model systems that are able to mimic the in vivo microenvironment are now highly sought after in cancer research. Antheraea mylitta silk fibroin protein matrices were investigated as potential biomaterial for in vitro tumor modeling. We compared the characteristics of MDA-MB-231 cells on A. mylitta, Bombyx mori silk matrices, Matrigel, and tissue culture plates. The attachment and morphology of the MDA-MB-231 cell line on A. mylitta silk matrices was found to be better than on B. mori matrices and comparable to Matrigel and tissue culture plates. The cells grown in all 3D cultures showed more MMP-9 activity, indicating a more invasive potential. In comparison to B. mod fibroin, A. mylitta fibroin not only provided better cell adhesion, but also improved cell viability and proliferation. Yield coefficient of glucose consumed to lactate produced by cells on 3D A. mylitta fibroin was found to be similar to that of cancer cells in vivo. LNCaP prostate cancer cells were also cultured on 3D A. mylitta fibroin and they grew as clumps in long term culture. The results indicate that A. mylitta fibroin scaffold can provide an easily manipulated microenvironment system to investigate individual factors such as growth factors and signaling peptides, as well as evaluation of anticancer drugs. (C) 2010 Elsevier Ltd. All rights reserved.
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