Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Review
Cell & Tissue Engineering
Aline Queiroz, Emmanuel Albuquerque-Souza, Leticia Miquelitto Gasparoni, Bruno Nunes de Franca, Cibele Pelissari, Marilia Trierveiler, Marinella Holzhausen
Summary: PDLSCs are considered a promising cell source for regenerating periodontium and have the potential to be used as an effective treatment for periodontitis.
WORLD JOURNAL OF STEM CELLS
(2021)
Article
Biochemistry & Molecular Biology
Oliwia Milek, Dino Tur, Lucia Ahcin, Olha Voitseshyna, Christian Behm, Oleh Andrukhov
Summary: This study investigated the impact of osteogenic differentiation on the immunomodulatory properties of hPDL-MSCs, and found that osteogenic differentiation reduced the ability of hPDL-MSCs to suppress CD4(+) T lymphocyte proliferation and enhanced this ability in the presence of IL-1 beta. Additionally, the expression and activity of indoleamine-2,3-dioxygenase and interleukin-8 were also affected by the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Maximilian Handke, Anastasia Rakow, Debora Singer, Lea Miebach, Frank Schulze, Sander Bekeschus, Janosch Schoon, Georgi I. Wassilew
Summary: Bone marrow obtained during periacetabular osteotomy (PAO) may serve as a promising novel source for BM-MSCs from young donors. Compared to THA patients, PAO patients are younger and BM-MSCs have higher proliferation capacity and secrete more growth factors.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alice Blufstein, Christian Behm, Barbara Kubin, Johannes Gahn, Xiaohui Rausch-Fan, Andreas Moritz, Oleh Andrukhov
Summary: The study showed that inflammatory stimuli reduced the expression of osteogenesis-related factors induced by 1,25-dihydroxyvitamin D-3 in human periodontal ligament stromal cells (hPDLSCs) under osteogenic conditions, while having minimal effect on the osteogenic differentiation.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Jehan J. El-Jawhari, Payal Ganguly, Elena Jones, Peter Giannoudis
Summary: This review discusses the application of bone marrow-derived mesenchymal stromal cells in treating osteonecrosis, including donor-related factors, the impact of aging on donor-related variability, and factors influencing the quantity and function of these cells.
BIOENGINEERING-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Raphael Gorodetsky, Wilhelm K. Aicher
Summary: MSCs from different sources can release pro-regenerative and anti-inflammatory factors, with hPSCs from the fresh human placenta being a preferred source for abundant cell harvesting. The f-hPSCs from neonate tissues of the placenta can undergo extended expansion with low risk of senescence, making them competent cells for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Christian Behm, Alice Blufstein, Johannes Gahn, Andreas Moritz, Xiaohui Rausch-Fan, Oleh Andrukhov
Summary: Human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs) have bi-directional interaction with CD4(+) T lymphocytes, and their immunomodulatory abilities are enhanced by 25-hydroxyvitamin D-3 (25(OH)D-3) through boosting the expression of immunomediators. The influence of 25(OH)D-3 on this interaction is still unknown.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ivana Okic Dordevic, Tamara Kukolj, Milena Zivanovic, Sanja Momcilovic, Hristina Obradovic, Andelija Petrovic, Slavko Mojsilovic, Drenka Trivanovic, Aleksandra Jaukovic
Summary: Periodontitis is a chronic bacterial disease that can lead to bone resorption and tooth loss. This study found that the antibiotic doxycycline can enhance the migration and osteogenic potential of periodontal ligament mesenchymal stem cells, while reducing the effects of the proinflammatory factor interleukin-17.
Article
Cell & Tissue Engineering
Xiaoru Xu, Shuang Song, Xiangdong Liu, Yuchao Zhou, Shaojie Shi, Guoqiang Zhao, Xingxing Wang, Xutao Chen, Wenshuang Zhao, Sijia Zhang, Wei Ma, Yingliang Song
Summary: Cell sheet technology utilizing adipose-derived stromal cells (ASCs) shows promise in promoting angiogenesis and may have valuable clinical applications.
STEM CELLS INTERNATIONAL
(2022)
Article
Medicine, General & Internal
Hideaki Kagami, Minoru Inoue, Hideki Agata, Izumi Asahina, Tokiko Nagamura-Inoue, Masataka Taguri, Arinobu Tojo
Summary: By optimizing the cell-processing protocol, stable bone regeneration can be achieved with comparable quality and less fluctuation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Alison J. Wilson, Emma Rand, Andrew J. Webster, Paul G. Genever
Summary: Many clinical trials involving mesenchymal stem/stromal cells lack comprehensive characterization data of the cellular population, including details on surface marker expression, viability, and differentiation. Functional assessment is very limited and does not always correlate with the likely mechanism of action.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Physiology
Yukun Jiang, Yuzhe Guan, Yuanchen Lan, Shuo Chen, Tiancheng Li, Shujuan Zou, Zhiai Hu, Qingsong Ye
Summary: The Piezo1 channel plays a critical role in mediating bone formation and osteoclastic activities during orthodontic tooth movement, particularly on the tension side. Inhibition of Piezo1 function leads to reduced expression of osteogenesis-associated transcription factors and disruptions in bone remodeling processes.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jeremy Boulestreau, Marie Maumus, Christian Jorgensen, Daniele Noel
Summary: Osteoarthritis is associated with a high number of senescent cells in joint tissues, which release senescence-associated secretory phenotype (SASP) factors contributing to the disease progression. Targeting senescent cells has shown improvement in OA features in mouse models. MSCs and MSC-EVs are promising treatments for OA, but their functions are affected by aging.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Medicine, General & Internal
Izumi Asahina, Hideaki Kagami, Hideki Agata, Masaki J. Honda, Yoshinori Sumita, Minoru Inoue, Tokiko Nagamura-Inoue, Arinobu Tojo
Summary: This study evaluated the safety and efficacy of bone tissue engineering using BMSCs in patients with severe atrophic alveolar bone. The results showed good long-term stability and safety of the regenerated bone, suggesting potential application in clinical practice. However, individual variations were observed, highlighting the need for further studies with larger sample sizes to confirm the efficacy of BMSCs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Tomoko Kobayashi, Daisuke Torii, Takanori Iwata, Yuichi Izumi, Masanori Nasu, Takeo W. Tsutsui
Review
Multidisciplinary Sciences
Risako Mikami, Koji Mizutani, Yoshiyuki Sasaki, Takanori Iwata, Akira Aoki
Article
Dentistry, Oral Surgery & Medicine
Takahiro Ikawa, Koji Mizutani, Takeaki Sudo, Chihiro Kano, Yuichi Ikeda, Tatsuya Akizuki, Hiroaki Kobayashi, Yuichi Izumi, Takanori Iwata
Summary: Electric ionic toothbrushes demonstrated significantly higher plaque removal rates than manual toothbrushes in the premolar and molar areas, while no significant difference was observed in the central incisor area.
INTERNATIONAL JOURNAL OF DENTAL HYGIENE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Koji Mizutani, Hidehiro Shioyama, Takanori Matsuura, Risako Mikami, Kohei Takeda, Yuichi Izumi, Akira Aoki, Takanori Iwata
Summary: This study found that minimally invasive periodontal surgery using EMD in patients with and without diabetes mellitus can lead to similar levels of clinical attachment gain and bone fill. This is the first documented study of successful periodontal tissue regeneration in patients with diabetes mellitus.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yuichi Ikeda, Ayako Kawada, Daiki Tanaka, Eri Ikeda, Hiroaki Kobayashi, Takanori Iwata
Summary: The study found that patients had fewer complaints with M-USSC compared to P-USSC during supragingival scaling, with significant improvements in patient complaints when using M-USSC.
INTERNATIONAL JOURNAL OF DENTAL HYGIENE
(2021)
Review
Chemistry, Physical
Shunsuke Fukuba, Munehiro Okada, Kohei Nohara, Takanori Iwata
Summary: This review provides a summary of available bone graft products worldwide and discusses the prospects of alloplastic bone substitutes in the market and trends.
Review
Biochemistry & Molecular Biology
Masahiro Hatasa, Sumiko Yoshida, Hirokazu Takahashi, Kenichi Tanaka, Yoshihito Kubotsu, Yujin Ohsugi, Takaharu Katagiri, Takanori Iwata, Sayaka Katagiri
Summary: Periodontal disease is an inflammatory disease caused by oral microorganisms, with studies showing it as a risk factor for systemic diseases. The relationship between NAFLD and periodontal disease remains unclear. Epidemiological studies suggest periodontal disease as a risk factor for NAFLD, with animal models showing acceleration of NAFLD progression by periodontopathic bacteria. Th17 may play a key role in explaining the relationship between the two diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Akira Aoki, Koji Mizutani, Risako Mikami, Yoichi Taniguchi, Yujin Ohsugi, Walter Meinzer, Yuichi Izumi, Takanori Iwata
Summary: Er:YAG laser-assisted pocket therapy can effectively and safely clean periodontal pockets, leading to significant clinical improvements in most cases without adverse side effects or complications.
CLINICAL ORAL INVESTIGATIONS
(2022)
Review
Biochemistry & Molecular Biology
Peiya Lin, Hiromi Niimi, Yujin Ohsugi, Yosuke Tsuchiya, Tsuyoshi Shimohira, Keiji Komatsu, Anhao Liu, Takahiko Shiba, Akira Aoki, Takanori Iwata, Sayaka Katagiri
Summary: Periodontitis is an inflammatory disease that involves the destruction of periodontal tissues. A new murine model using ligation has been effective in simulating bone loss and tissue destruction. The activation of osteoclasts, upregulation of pro-inflammatory factors, and excessive immune response are key factors in periodontal disruption. Multiple genes play a role in the complexity of periodontitis pathogenesis. Novel treatment methods have shown promise in the ligature-induced periodontitis model in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Hiroyuki Saida, Shunsuke Fukuba, Takahiko Shiba, Keiji Komatsu, Takanori Iwata, Hiroshi Nitta
Summary: This case report demonstrates a novel staged surgical approach for treating furcation defects with insufficient keratinized mucosa, which led to successful periodontal regeneration and clinical improvement.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Koji Mizutani, Prima Buranasin, Risako Mikami, Kohei Takeda, Daisuke Kido, Kazuki Watanabe, Shu Takemura, Keita Nakagawa, Hiromi Kominato, Natsumi Saito, Atsuhiko Hattori, Takanori Iwata
Summary: This review found that the adjunctive use of antioxidants in periodontal therapy can significantly improve periodontal parameters in patients with type 2 diabetes, especially in the group treated with melatonin. However, there is still limited research on this topic, and more well-designed RCTs are needed for further investigation.
Article
Biochemistry & Molecular Biology
Yuka Tanaka-Takemura, Yoshinori Arisaka, Masahiro Hakariya, Hiroki Masuda, Ryo Mikami, Ruriko Sekiya-Aoyama, Takanori Iwata, Tetsuya Yoda, Takashi Ono, Nobuhiko Yui
Summary: Surface properties of biomaterials, such as zeta potential and molecular mobility, play important roles in influencing macrophage responses. The zeta potential affects the initial spreading of cells, while molecular mobility governs the subsequent spreading process. Molecular mobility is the main factor modulating the inflammatory activity in macrophages.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Masahiro Hakariya, Yoshinori Arisaka, Hiroki Masuda, Tetsuya Yoda, Takanori Iwata, Nobuhiko Yui
Summary: Cancer cells respond to physical cues from the microenvironment by altering migration and chemosensitivity. The molecular mobility of polyrotaxane surfaces affects cellular spreading, migration, and chemosensitivity in lung, pancreatic, and breast cancer cells.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hiroki Masuda, Yoshinori Arisaka, Masahiro Hakariya, Takanori Iwata, Tetsuya Yoda, Nobuhiko Yui
Summary: This study evaluates the effects of molecular mobility of polyrotaxane surfaces on subcellular YAP localization and oxidative stress-induced cell senescence in human mesenchymal stem cells (HMSCs). The results suggest that the molecular mobility of polyrotaxane surfaces regulates subcellular YAP localization, thereby protecting HMSCs from oxidative stress-induced cell senescence. Applying the molecular mobility of polyrotaxane surfaces can provide insights into the prevention and treatment of diseases caused by oxidative stress.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Polymer Science
Masahiro Hakariya, Yoshinori Arisaka, Hiroki Masuda, Tetsuya Yoda, Atsushi Tamura, Takanori Iwata, Nobuhiko Yui
Summary: Researchers have designed a bilayer hydrogel structure with PRX and collagen layers to achieve rapid and strong adhesion to target tissues. In in vivo experiments in mice, the bilayer hydrogel demonstrated superior adhesion properties.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.