Article
Multidisciplinary Sciences
Juan C. Fraire, Elnaz Shaabani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens, Dawei Hua, Jana Ramon, Laurens Raes, Eduardo Bolea-Fernandez, Toon Brans, Frank Vanhaecke, Peter Borghgraef, Chaobo Huang, Felix Sauvage, Tamara Vanhaecke, Joery De Kock, Ranhua Xiong, Stefaan De Smedt, Kevin Braeckmans
Summary: Biolistic delivery with light-triggered self-assembled nanobombs can efficiently transfect mammalian cells by propelling smaller nanoprojectiles through the cell membrane using vapor bubble formation. It outperforms electroporation in terms of transfection yield.
NATURE COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Victoria L. Messerschmidt, Uday Chintapula, Aneetta E. Kuriakose, Samantha Laboy, Thuy Thi Dang Truong, LeNaiya A. Kydd, Justyn Jaworski, Zui Pan, Hashem Sadek, Kytai T. Nguyen, Juhyun Lee
Summary: Notch signaling is crucial for embryonic development and organ regeneration, with loss of signal resulting in maldevelopment. Encapsulating Notch plasmid in nanoparticles made of poly(lactic-co-glycolic acid) can reestablish downstream signaling, showing non-toxicity, stability, compatibility with blood, and successful transfection of HUVECs.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Physiology
Victoria L. Messerschmidt, Uday Chintapula, Fabrizio Bonetesta, Samantha Laboy-Segarra, Amir Naderi, Kytai T. Nguyen, Hung Cao, Edward Mager, Juhyun Lee
Summary: In the era of advanced nanomaterials, the use of nanoparticles for biomedical research is important. This study investigated the delivery of a NICD-encoded plasmid using PLGA nanoparticles and the potential toxic effects. The results showed successful delivery of the plasmid and increased expression of Notch signaling related genes. The nanoparticles caused fewer malformations compared to the positive control, ZnO nanoparticles.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemical Research Methods
Andrew Hamann, Angela K. Pannier
Summary: Although human mesenchymal stem cells (hMSCs) have not yet been approved by the FDA for clinical use, research is ongoing to develop gene delivery methods for therapeutic applications. Strategies, such as innovative carriers and chemical and physical priming, have been developed to improve transfection efficiency in hMSCs. These strategies aim to optimize cell phenotypes for enhanced immunomodulation and tissue regeneration, as well as for functions like tumor-killing and tissue engineering.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
M. Torres-Ramos, M. F. Martin-Marquez, Maria del Carmen Leal-Moya, Suresh Ghotekar, Jorge Alberto Sanchez-Burgos, Alejandro Perez-Larios
Summary: This paper reports the green synthesis of PLGA-TiO2 nanocomposite using a natural extract and its characterization. The study used UV-visible spectrometry, scanning electron microscopy, infrared spectroscopy, and X-ray diffraction to analyze the properties of the nanoparticles. The results showed that terpenes enhance the synthesis and stabilization of titanium dioxide nanoparticles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Antonella Piscioneri, Sabrina Morelli, Tiziana Ritacco, Michele Giocondo, Rafael Penaloza, Enrico Drioli, Loredana De Bartolo
Summary: Biomaterial surface modification through the introduction of defined patterns of topography helps study cell behavior in response to geometrical cues. The lithographic molding technique is widely used for conferring biomaterial surface cues and enhancing the performance of biomedical devices. Different topographical features, such as channels, pillars, and pits, were investigated for their effects on different cell types, revealing distinct cell responses and interactions.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Seongyeon Kim, Le Thi Thuy, Jeil Lee, Joon Sig Choi
Summary: In this study, RRHRH oligopeptide was successfully conjugated to the surface of PAMAM G2, forming RRHRH-PAMAM G2 for gene delivery. The results showed that RRHRH-PAMAM G2 had small nanoparticle size, positive charge, high transfection efficiency, and biocompatibility with cell lines, indicating its potential as a gene carrier for gene therapy.
Article
Chemistry, Multidisciplinary
Yanzhen Sun, Xiaoli Ma, Xiaodong Jing, Hao Hu
Summary: In this study, three-dimensional spherical PMAMA dendrimers are conjugated onto the surface of cellulose nanocrystals (CNCs) to create a novel gene delivery system called CNC-PAMAM. This system shows high transfection efficiency and low toxicity, effectively delivering the suicide gene to the tumor site.
Article
Pharmacology & Pharmacy
Treniece L. Terry, Brittany E. Givens, Andrea Adamcakova-Dodd, Peter S. Thorne, Victor G. J. Rodgers, Aliasger K. Salem
Summary: Encapsulating genetic material into biocompatible polymeric microparticles can improve gene transfection efficiency and reduce inflammatory responses. Factors such as the N/P ratio of PEI to DNA and PEGylation play roles in enhancing transgene expression and reducing inflammation. The study demonstrated the advantages of PEGylation and lack of porosity in microparticles for faster release of genetic cargo and decreased inflammatory response.
Article
Immunology
Muriel F. Gusta, Michael J. Edel, Vivian A. Salazar, Belen Alvarez-Palomo, Manel Juan, Massimo Broggini, Giovanna Damia, Paolo Bigini, Alessandro Corbelli, Fabio Fiordaliso, Alexander Barbul, Rafi Korenstein, Neus G. Bastus, Victor Puntes
Summary: Gene therapy holds promise to cure diseases, but efficient carriers for gene delivery are needed. 'Non-viral' vectors, such as cationic polymers, have gained popularity but suffer from high toxicity. Nanoconjugation can eliminate this toxicity, but optimizing nanovector size and charge is not the only barrier to efficient gene delivery.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Diviya Santhanes, Alex Wilkins, Huiming Zhang, Robert John Aitken, Mingtao Liang
Summary: Lipid/polymer hybrid nanoparticles loaded with red fluorescent protein encoded plasmid DNA were successfully formulated using microfluidics. These nanoparticles showed high encapsulation efficiency and transfection efficacy, demonstrating their potential for clinical applications in DNA therapeutics.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Nanoscience & Nanotechnology
Pardis Moradi, Akbar Hasanzadeh, Fatemeh Radmanesh, Saideh Rajai Daryasarei, Elaheh Sadat Hosseini, Jafar Kiani, Ali Shahbazi, Helena Nourizadeh, Maryam Eslami, Akbar Dorgalaleh, Maryam Sahlolbei, Michael R. Hamblin, Mahdi Karimi
Summary: A novel nonvehicle nanoparticle (PEI1.8k-Arg) has been designed for efficient and safe delivery of CRISPR plasmid. The nanoparticle exhibits higher transfection efficiency compared to traditional carriers and successfully delivers p/CRISPR into a variety of cells, including brain tissue.
Article
Polymer Science
Beste Cagdas Tunali, Omer Akturk, Durukan Sahingoz, Mustafa Turk, Ayten Celebi Keskin
Summary: In this study, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized using the pulsed laser ablation in liquid (PLAL) technique, and then combined with Bombyx mori silk fibroin (SF) to form nanocomposites (PEI-SF-AuNPs). The biocompatibility of PEI-AuNPs and PEI-SF-AuNPs in breast cancer cells MCF-7 and MDA-MB231 were tested, and it was found that PEI-AuNP1 showed the least cytotoxicity. Therefore, PEI-AuNP1 nanocomposites can be suggested as siRNA carriers in gene-based breast cancer therapies.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Chong Liu, Zhaopei Guo, Huimin Feng, Lin Lin, Yuan Cui, Yanhui Li, Huayu Tian
Summary: In this study, a series of hyperbranched copolymers PEI-co-PPhe were synthesized for gene and drug codelivery, showing promising synergistic therapeutic effects against cancer cells. The appropriate length of PPhe grafted on PEI improved gene transfer efficiency and decreased cytotoxicity, enabling effective codelivery of gene and drug into cancer cells.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Biophysics
Sadegh Dehghani, Mona Alibolandi, Zeinab Amiri Tehranizadeh, Reza Kazemi Oskuee, Rahim Nosrati, Fatemeh Soltani, Mohammad Ramezani
Summary: This study developed a novel peptide-based non-viral carrier decorated with aptamer to overcome specific gene delivery barriers, showing capabilities in specific cell targeting, overcoming cellular gene delivery barriers, and mediating efficient gene transfection. The KLN/pDNA/aptamer nanoparticles demonstrate remarkable potential for therapeutically demanding applications through concept design and formation of multi-functionalized carriers.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.