期刊
BIOMATERIALS
卷 32, 期 4, 页码 979-984出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.10.011
关键词
Staphylococcus epidermidis; U2OS osteoblasts; Biomaterial-associated infections; Co-culture; PEG coatings; Race for the surface
资金
- University of Groningen-University Medical Center Groningen, Groningen, The Netherlands
Biomaterial-associated infections (BAI) remain a serious clinical complication, often arising from an inability of host tissue-implant integration to out-compete bacterial adhesion and growth. A commercial polymer coating based on polyethylene glycol (PEG), available in both chemically inert and NHS-activated forms (OptiChem), was compared for simultaneous growth of staphylococci and osteoblasts. In the absence of staphylococci, osteoblasts adhered and proliferated well on glass controls and on the NHS-reactive PEG-based coating over 48 h, but not on the inert PEG coating. Staphylococcal growth was low on both PEG-based coatings. When staphylococci were pre-adhered on surfaces for 1.5 h to mimic peri-operative contamination, osteoblast growth and spreading was reduced on glass but virtually absent on both reactive and inert PEG-based coatings. Thus although NHS-reactive, PEG-based coatings stimulated tissue cell interactions in the absence of contaminating staphylococci, the presence of adhering staphylococci eliminated osteoblast adhesion advantages on the PEG surface. This study demonstrates the importance of using bacterial and cellular co-cultures compared to monocultures when assessing functionalized biomaterials coatings for infectious potential. (c) 2010 Elsevier Ltd. All rights reserved.
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