Article
Biochemistry & Molecular Biology
Marie Maumus, Guillaume Fonteneau, Maxime Ruiz, Said Assou, Hassan Boukhaddaoui, Philippe Pastoureau, Frederic De Ceuninck, Christian Jorgensen, Daniele Noel
Summary: NMB is a newly identified chondroinductive bioactive factor that upregulates the key chondrogenic transcription factor Sox9 through modulation of the Ca2+ signaling pathway and calcineurin activity. NMB plays a significant role in chondrogenesis.
CELL AND BIOSCIENCE
(2021)
Article
Materials Science, Biomaterials
Jiayi Zhu, Wanqing Lun, Qi Feng, Xiaodong Cao, Qingtao Li
Summary: The mechanical stiffness of the ECM affects YAP activity, which in turn regulates the differentiation of stem cells in cartilage tissue development and regeneration. However, the mechanisms underlying scaffold-associated mechanobiology during chondrogenesis initiation and hyaline cartilage maintenance are not well understood. In this study, we investigated the relationship between YAP subcellular localization and changes in cartilage structure during early postnatal growth. We also studied the effect of scaffold stiffness on hyaline cartilage formation by mesenchymal stem cells and the role of YAP activity.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Biochemistry & Molecular Biology
Charindu K. I. Ranmuthu, Chanuka D. S. Ranmuthu, Chalukya K. Wijewardena, Matthew K. T. Seah, Wasim S. Khan
Summary: This systematic review explores the effect of hypoxia on chondrogenic differentiation of human adult mesenchymal stromal cells (MSCs). Most studies suggest that hypoxia during the expansion or differentiation phase enhances some markers of chondrogenic differentiation in adult MSCs. However, results are inconsistent and not always confirmed at the protein level. Factors such as MSC source, donor variability, hypoxia level, exposure time, and culture methodology contribute to these inconsistent findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Polymer Science
Elena Gabusi, Enrico Lenzi, Cristina Manferdini, Paolo Dolzani, Marta Columbaro, Yasmin Saleh, Gina Lisignoli
Summary: Autophagy is a cellular process that maintains cell homeostasis by activating a specific pathway and providing necessary factors in stressful and physiological situations. In a 3D hydrogel, adipose-derived mesenchymal stromal cells (ASCs) showed increased expression of autophagic markers and features. Autophagy plays a crucial role in ASCs chondrogenic differentiation by providing energy and necessary factors.
Review
Chemistry, Medicinal
Madhan Jeyaraman, Sathish Muthu, Prakash Gangadaran, Rajni Ranjan, Naveen Jeyaraman, Gollahalli Shivashankar Prajwal, Prabhu Chandra Mishra, Ramya Lakshmi Rajendran, Byeong-Cheol Ahn
Summary: The periosteum, rich in pluripotent stem cells, plays a crucial role in fracture healing and remodeling. While its potential in tissue engineering is recognized, further research is needed to explore the bone-forming capabilities of its stem cells.
Article
Cell & Tissue Engineering
K. Futrega, E. Music, P. G. Robey, S. Gronthos, R. Crawford, S. Saifzadeh, T. J. Klein, M. R. Doran
Summary: The study characterized ovine BMSC and evaluated their ability to repair cartilage defects, concluding that micro-pellets manufactured from ovine articular chondrocytes were more effective than those from ovine BMSC. While ovine BMSC could form cartilage-like tissue in vitro, their effective application in cartilage repair will require successful mitigation of hypertrophy and tissue integration issues.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
C. Schneider, P. Dungel, E. Priglinger, M. Danzer, B. Schadl, S. Nurnberger
Summary: LED devices of blue, green, or red light were used to stimulate adipose-derived stromal/stem cells towards a chondrogenic phenotype. The reactions to PBM were wavelength-dependent and more pronounced during the expansion phase. PBM can enhance or hamper chondrogenesis of ASC depending on treatment parameters and intrinsic cellular potential.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Review
Cell Biology
Alexander Rauch, Susanne Mandrup
Summary: Stromal progenitor cells contribute to the maintenance of tissue homeostasis in different organs and can differentiate into various cell types in vitro. Recent omics and single-cell studies provide insights into the gene regulatory networks that drive lineage determination and cell differentiation, which have important implications for understanding human diseases and the development of cell-based therapies.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Article
Oncology
Shiqi Xiang, Zixuan Lin, Meagan J. J. Makarcyzk, Kanyakorn Riewruja, Yiqian Zhang, Xiurui Zhang, Zhong Li, Karen L. Clark, Eileen Li, Silvia Liu, Tingjun Hao, Madalyn R. R. Fritch, Peter G. G. Alexander, Hang Lin
Summary: This study elucidates the difference between hiMPCs and hMSCs in response to TGF beta and presents a robust method for obtaining low hypertrophy hiMPCs-derived cartilage. The results also indicate the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cell & Tissue Engineering
Maiko Sezaki, Subinoy Biswas, Sayuri Nakata, Motohiko Oshima, Shuhei Koide, Nicole Pui Yu Ho, Nobukazu Okamoto, Takeshi Miyamoto, Atsushi Iwama, Hitoshi Takizawa
Summary: This study characterizes the heterogeneity of hMSCs and their ability to efficiently form humanized ossicles in good donors by correlating with the frequency and functionality of chondrocyte progenitors. CD271(+)CD51(+) stromal cell subset was found to possess enhanced hMSC activity, with PALLADIN-negative cells displaying the greatest CFU-F potential in subsets enriched for greater chondrogenic ability. The findings contribute to better understanding and isolation of ossicle-forming hMSCs for optimized engineering of human bone organs.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Engineering, Biomedical
Jiabin Zhang, Ming Zhang, Rongcai Lin, Yuguang Du, Liming Wang, Qingqiang Yao, Andrew Zannettino, Hu Zhang
Summary: Stem cell therapy using magnetic scaffolds has been developed to treat osteoarthritis. The magnetic scaffolds enhance chondrogenesis of mesenchymal stem cells through mechanical stimulation and magnetic field effects. The fiber topography of the scaffolds can be tuned with different coatings on magnetic nanoparticles, which selectively up-regulate chondrogenesis or osteogenesis-related genes.
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Md Shaffiulah Shajib, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: This study evaluated the ability of a BMP inhibitor, LDN 193189, to prevent BMSC hypertrophy. The results showed that LDN 193189 did not prevent hypertrophy, but it was effective in countering mineralization and adipogenesis.
Article
Cell & Tissue Engineering
Daniele Zuncheddu, Elena Della Bella, Dalila Petta, Cecilia Bartschi, Sonja Hackel, Moritz C. Deml, Martin J. Stoddart, Sibylle Grad, Valentina Basoli
Summary: This study investigates the effects of glucose and fructose on the chondrogenic differentiation of BMSCs. The results show that fructose may enhance chondrogenic differentiation, while decreasing glucose concentration may decrease differentiation yield. The study also reveals the expression and regulation of GLUT3 and GLUT5 in BMSCs during chondrogenesis. The findings provide valuable insights into the sugar metabolism of BMSCs during cartilage formation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Chemistry, Physical
O. Thomas, L. Lemaire, J. P. Benoit, P. Saulnier
COLLOID AND POLYMER SCIENCE
(2020)
Article
Chemistry, Physical
Thao-Quyen Nguyen-Pham, Lazhar Benyahia, Guillaume Bastiat, Jeremie Riou, Marie-Claire Venier-Julienne
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Article
Immunology
Mazigh Fares, Marielle Cochet-Bernoin, Gaelle Gonzalez, Claudia N. Montero-Menei, Odile Blanchet, Alexandra Benchoua, Claire Boissart, Sylvie Lecollinet, Jennifer Richardson, Nadia Haddad, Muriel Coulpier
JOURNAL OF NEUROINFLAMMATION
(2020)
Article
Biophysics
Deniz Ugur, Virginie Sottile, Claudia N. Montero-Menei, Frank Boury, Mischa Zelzer
Article
Clinical Neurology
Anne-Catherine Bachoud-Levi
MOVEMENT DISORDERS
(2020)
Article
Materials Science, Biomaterials
Assia Rmaidi, Mischa Zelzer, Laurence Sindji, Raphael Dima, Frank Boury, Nicolas Delorme, Claudia N. Montero-Menei
Summary: This study aimed to characterize the physicochemical properties of two polymeric microsphere (MS) systems and evaluate the effect of laminin and poly-n-lysine coatings on stem cell behavior. The results showed that PLGA microspheres promoted protein adsorption and cell adhesion, while PLGA-P188-PLGA microspheres had less protein adsorption and resulted in round cell morphology. However, the long-term cell behavior, including cell number, differentiation profile, and ECM secretion, were not significantly influenced by the type of microcarrier used. The study suggests that the ECM molecules surrounding the microcarrier/cell constructs play a role in converging cell behavior over time.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Pauline Rozier, Marie Maumus, Claire Bony, Alexandre Thibault Jacques Maria, Florence Sabatier, Christian Jorgensen, Philippe Guilpain, Daniele Noel
Summary: This study demonstrates that T beta-Fb is a relevant model for mimicking the main characteristics of SSc fibroblasts and investigating the mechanism of action of ASCs. Furthermore, ASC-derived EVs were shown to be more effective than parental cells, suggesting that the TGF beta 1-induced pro-fibrotic environment may alter the function of ASCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marie Maumus, Guillaume Fonteneau, Maxime Ruiz, Said Assou, Hassan Boukhaddaoui, Philippe Pastoureau, Frederic De Ceuninck, Christian Jorgensen, Daniele Noel
Summary: NMB is a newly identified chondroinductive bioactive factor that upregulates the key chondrogenic transcription factor Sox9 through modulation of the Ca2+ signaling pathway and calcineurin activity. NMB plays a significant role in chondrogenesis.
CELL AND BIOSCIENCE
(2021)
Review
Cell Biology
Amel Nasri, Florent Foisset, Engi Ahmed, Zakaria Lahmar, Isabelle Vachier, Christian Jorgensen, Said Assou, Arnaud Bourdin, John De Vos
Summary: Mesenchymal cells play crucial roles in lung development, regeneration, and diseases, especially in chronic obstructive pulmonary disease.
Article
Medicine, General & Internal
Radjiv Goulabchand, Audrey Gabelle, Xavier Ayrignac, Nicolas Malafaye, Pierre Labauge, Daniele Noel, Jacques Morel, Camille Roubille, Lucie Barateau, Philippe Guilpain, Thibault Mura
Summary: A nationwide retrospective study in France using the health insurance database showed that patients with primary Sjogren's syndrome (pSS) have a higher risk of hospitalization for neurological diseases, including dementia, multiple sclerosis (MS), encephalitis, and peripheral neuropathy.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Yujie Song, Christian Jorgensen
Summary: This review highlights the potential of mesenchymal stromal cells (MSCs) in treating osteoarthritis (OA), focusing on their chondrogenic and immunomodulatory potential. The review provides an overview of the effects of MSCs in preclinical and clinical studies of OA treatment, comparing different MSC sources. Furthermore, the review summarizes the current evidence for the assessment of cartilage in OA using magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy.
Review
Biochemistry & Molecular Biology
Giuliana Minani Bertolino, Marie Maumus, Christian Jorgensen, Daniele Noel
Summary: Extracellular vesicles (EVs) isolated from induced pluripotent stem cells (iMSCs) show promise in regenerative medicine for the treatment of various diseases. However, the heterogeneity between MSC donors may lead to batch-to-batch variability in the use of EVs as cell-free therapeutic agents.
Article
Cell & Tissue Engineering
Noemie Petitjean, Patrick Canadas, Christian Jorgensen, Pascale Royer, Simon Le Floc'h, Daniele Noel
Summary: This study aimed to investigate the impact of mechanical stimulation on chondrocytes derived from mesenchymal stromal cells in their own extracellular matrix.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Polymer Science
Laurine Valot, Marie Maumus, Luc Brunel, Jean Martinez, Muriel Amblard, Daniele Noel, Ahmad Mehdi, Gilles Subra
Summary: Researchers have developed a biomimetic chemical hydrogel based on silylated collagen-mimetic synthetic peptides, which successfully encapsulated MSCs and improved mechanical properties, resulting in prolonged survival of the embedded cells and enhanced expression of chondrocyte markers. This long-standing hybrid hydrogel shows promise as a synthetic and modular scaffold for cartilage tissue engineering.
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.