期刊
BIOMATERIALS
卷 31, 期 34, 页码 8847-8853出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.08.004
关键词
Islets of Langerhans; Bioartificial pancreas; Agarose; Soluble complement receptor 1 (sCR1); Microencapsulation; Immuno-isolation
资金
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan [21650118]
- Ministry of Health, Labor, and Welfare of Japan [H20-007]
- JICA
- [21240051]
- Grants-in-Aid for Scientific Research [21650118] Funding Source: KAKEN
The transplantation of islets of Langerhans has been successfully applied to the treatment of insulin-dependent diabetes. However, a shortage of human donors is the hardest obstacle to overcome. We aimed to develop a bioartificial pancreas that can realize xeno-islet transplantation. The islets were encapsulated in agarose microbeads carrying the soluble domain of human complement receptor 1 (sCR1), which is an effective inhibitor of the classical and alternative complement activation pathways. When naked rat islets were cultured in rabbit serum, large amounts of insulin leaked from the damaged islets over the course of a few days incubation, but no damaged cells were observed among islets in sCR1-agarose microbeads cultured in rabbit serum for 4 days. Although low levels of insulin were detected in the rabbit serum, the insulin did not leak from damaged beta-cells, it was physiological insulin secreted by the beta-cells. (C) 2010 Elsevier Ltd. All rights reserved.
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