Article
Biotechnology & Applied Microbiology
Pedro Silva Couto, Dale J. J. Stibbs, Marco C. C. Rotondi, Yasuhiro Takeuchi, Qasim A. A. Rafiq
Summary: This study demonstrates a scalable manufacturing process using a microcarrier-bioreactor system for the expansion of gene-modified human mesenchymal stromal cells (hMSCs). The transduced and expanded cells met the criteria established by the International Society for Cell and Gene Therapy (ISCT). This work provides a foundation for the scalable manufacturing of gene-modified hMSCs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
E. L. Roberts, B. D. Abraham, T. Dang, E. Gysel, S. Mehrpouyan, A. H. Alizadeh, T. G. Koch, M. S. Kallos
Summary: Equine mesenchymal stromal cells (eMSCs) have shown promise in treating equine joint injuries, but challenges in biomanufacturing exist. This study demonstrates the scalability of a bioprocess for equine cord blood MSCs using microcarriers in VW bioreactors, achieving clinically relevant cell numbers while maintaining cell phenotype and functionality.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Erin L. Roberts, Sarah I. M. Lepage, Thomas G. Koch, Michael S. Kallos
Summary: This study investigated the development of an expansion process using Vertical-Wheel bioreactors for the cultivation of equine cord blood-derived MSCs. The results showed that VW bioreactors with appropriate microcarriers could produce large numbers of cells with reproducibility and homogeneity, making them suitable for cell therapy.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Alvin W. Nienow, Halina Murasiewicz, Andrzej W. Pacek, Christopher J. Hewitt, Karen Coopman
Summary: The study demonstrated the use of perfluorocarbon liquid microcarriers for expanding and recovering human mesenchymal stem/stromal cells, showing that cells can successfully attach, proliferate, and maintain quality on these liquid carriers.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Lihui Peng, Julien E. Gautrot
Summary: This study demonstrates that bioemulsions formed of microdroplets stabilized by protein nanosheets are well-suited for scale up of adherent stem cells like MSCs. Phenotyping of MSCs cultured on these bioemulsions, solid microcarriers, and classic 2D tissue culture polystyrene over multiple passages shows comparable phenotypes, although stemness is lost at late passages. This validates the use of bioemulsions for long term expansion of adherent stem cells and opens possibilities for novel 3D bioreactors based on microdroplet microcarriers.
MATERIALS TODAY BIO
(2021)
Article
Materials Science, Biomaterials
Gilad Doron, Levi B. Wood, Robert E. Guldberg, Johnna S. Temenoff
Summary: This study investigated the impact of microcarrier type on the secretory activity of genetically modified MSCs and identified changes in MAPK pathway signaling. The results showed that culture on stiffer microcarriers promoted the secretion of IL-1Ra and in vitro angiogenic activity, while culture on both stiffer and softer microcarriers altered the secretion of other factors. Additionally, culture on softer microcarriers increased the gene expression and secretion of hepatocyte growth factor.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Tristan Le Clainche, Anaick Moisan, Jean-Luc Coll, Veronique Martel-Frachet
Summary: The study compared different shapes and functionalizations of microcarriers for A-MSC expansion, finding that disc-shaped microcarriers (GhaterDisc-3) were the best alternative with a high yield of around 100% and the ability to maintain A-MSC characteristics. Furthermore, these microcarriers are free from any animal derived components, making them suitable for GMP compliant large-scale A-MSC production for biomedical applications.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Biomedical
Meadhbha Brennan, David S. Monahan, Benedicte Brulin, Sara Gallinetti, Paul Humbert, Christina Tringides, Cristina Canal, Maria Pau Ginebra, Pierre Layrolle
Summary: In this study, the healing capacity of CDHA administering MSCs to bone defects was evaluated for the first time and compared with sintered beta-TCP constructs. Greater cell infiltration occurred in CDHA scaffolds, and after 8 weeks in vivo, MSC engraftment was higher in CDHA compared to beta-TCP, as was bone in-growth.
ACTA BIOMATERIALIA
(2021)
Article
Biochemical Research Methods
Ee Xien Ng, Ming Wang, Shu Hui Neo, Ching Ann Tee, Chia-Hung Chen, Krystyn J. Van Vliet
Summary: A dissolvable gelatin-based microcarrier is developed for MSC expansion, showing high cell attachment efficiency, proliferation rate, and reduced cell loss during harvesting. MSCs cultured on gelatin microcarriers maintain trilineage differentiation with higher chondrogenic and osteogenic differentiation efficiency compared to traditional microcarriers.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Polymer Science
Bryan Falcones, Hector Sanz-Fraile, Esther Marhuenda, Irene Mendizabal, Ignacio Cabrera-Aguilera, Nanthilde Malandain, Juan J. Uriarte, Isaac Almendros, Daniel Navajas, Daniel J. Weiss, Ramon Farre, Jorge Otero
Summary: Mesenchymal stromal cell (MSC) therapy for acute respiratory diseases relies on MSCs' secretion of paracrine factors. Preconditioning the MSCs by growing them in a lung-derived extracellular matrix (ECM) can enhance their therapeutic efficacy. Bioink based on decellularized porcine lung ECM hydrogels supports 3D culturing of lung-resident MSCs without additional crosslinking, leading to improved cell-matrix interactions and increased expression of key receptors. This approach shows promise for enhancing MSC preconditioning for therapeutic applications.
Article
Pharmacology & Pharmacy
Yoshiki Ando, Fei-Chien Chang, Matthew James, Yang Zhou, Miqin Zhang
Summary: In this study, three-dimensional chitosan scaffolds are developed as microcarriers for the prolonged expansion of human neural stem cells (hNSCs) in bioreactors. The chitosan scaffolds exhibit desirable microstructural characteristics and mechanical properties, allowing for hNSC expansion in dynamic conditions for up to three weeks. This microcarrier approach has the potential for mass production of hNSCs in a three-dimensional setting.
Article
Pharmacology & Pharmacy
David Sanchez-Porras, Manuel Caro-Magdaleno, Carmen Gonzalez-Gallardo, Oscar Dario Garcia-Garcia, Ingrid Garzon, Victor Carriel, Fernando Campos, Miguel Alaminos
Summary: This study evaluated four decellularization protocols for porcine limbus, finding that the method using 0.1% SDS on half-thickness limbus best preserved transparency and extracellular matrix composition. Recellularization with SIRC and hADSCs produced stratified epithelium expressing limbal markers and basal lamina markers, showing similarities to native limbus. These findings suggest the potential use of these limbal substitutes in the future.
Review
Biochemistry & Molecular Biology
Mariangela Di Vincenzo, Federico Diotallevi, Silvia Piccirillo, Gianluca Carnevale, Annamaria Offidani, Anna Campanati, Monia Orciani
Summary: Micro RNAs (miRNAs), a type of non-coding RNA, interact with specific target mRNAs through base pairing, affecting their translation and stability. MiRNAs play a crucial role in regulating the fate of mesenchymal stromal cells (MSCs) in various skin diseases, both inflammatory and neoplastic. However, the exact role of miRNAs in these diseases is still a matter of debate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Raphael Gorodetsky, Wilhelm K. Aicher
Summary: MSCs from different sources can release pro-regenerative and anti-inflammatory factors, with hPSCs from the fresh human placenta being a preferred source for abundant cell harvesting. The f-hPSCs from neonate tissues of the placenta can undergo extended expansion with low risk of senescence, making them competent cells for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Ok-Hyeon Kim, Jun-Hyung Park, Jong-In Son, Ok-Ja Yoon, Hyun-Jung Lee
Summary: The study demonstrates that silk fibroin scaffolds can enhance the anti-inflammatory and immunomodulatory functions of mesenchymal stromal cells, showing potential benefits for the treatment of inflammatory disorders.
Article
Pharmacology & Pharmacy
Juan Aparicio-Blanco, Victor Sebastian, Jean P. Benoit, Ana I. Torres-Suarez
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2019)
Article
Parasitology
Gabriela V. Ullio Gamboa, Patricia E. Pensel, Maria C. Elissondo, Sergio F. Sanchez Bruni, Jean-Pierre Benoit, Santiago D. Palma, Daniel A. Allemandi
EXPERIMENTAL PARASITOLOGY
(2019)
Article
Oncology
Laura Pinton, Elena Masetto, Marina Vettore, Samantha Solito, Sara Magri, Marta D'Andolfi, Paola Del Bianco, Giovanna Lollo, Jean-Pierre Benoit, Hideho Okada, Aaron Diaz, Alessandro Della Puppa, Susanna Mandruzzato
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2019)
Article
Pharmacology & Pharmacy
M. L. Formica, G. V. Ullio Gamboa, L. Tartara, J. D. Luna, J. P. Benoit, S. D. Palma
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Parasitology
Julia Fabbri, Juan Pablo Espinosa, Patricia Eugenia Pensel, Sandra Karina Medici, Gabriela Ullio Gamboa, Jean Pierre Benoit, Maria Celina Elissondo
Article
Immunology
Mazigh Fares, Marielle Cochet-Bernoin, Gaelle Gonzalez, Claudia N. Montero-Menei, Odile Blanchet, Alexandra Benchoua, Claire Boissart, Sylvie Lecollinet, Jennifer Richardson, Nadia Haddad, Muriel Coulpier
JOURNAL OF NEUROINFLAMMATION
(2020)
Article
Biotechnology & Applied Microbiology
Laura Pinton, Sara Magri, Elena Masetto, Marina Vettore, Ilaria Schibuola, Vincenzo Ingangi, Ilaria Marigo, Kevin Matha, Jean-Pierre Benoit, Alessandro Della Puppa, Vincenzo Bronte, Giovanna Lollo, Susanna Mandruzzato
JOURNAL OF NANOBIOTECHNOLOGY
(2020)
Article
Pharmacology & Pharmacy
Kevin Matha, Giovanna Lollo, Giuseppe Taurino, Renaud Respaud, Ilaria Marigo, Molood Shariati, Ovidio Bussolati, An Vermeulen, Katrien Remaut, Jean-Pierre Benoit
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2020)
Article
Nanoscience & Nanotechnology
Molood Shariati, Giovanna Lollo, Kevin Matha, Benedicte Descamps, Christian Vanhove, Leen Van de Sande, Wouter Willaert, Lieve Balcaen, Frank Vanhaecke, Jean-Pierre Benoit, Wim Ceelen, Stefaan C. De Smedt, Katrien Remaut
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Biophysics
Deniz Ugur, Virginie Sottile, Claudia N. Montero-Menei, Frank Boury, Mischa Zelzer
Article
Clinical Neurology
Anne-Catherine Bachoud-Levi
MOVEMENT DISORDERS
(2020)
Article
Pharmacology & Pharmacy
Saikrishna Kandalam, Pauline De Berdt, Bernard Ucakar, Kevin Vanvarenberg, Caroline Bouzin, Viridiane Gratpain, Anibal Diogenes, Claudia N. Montero-Menei, Anne des Rieux
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Review
Pharmacology & Pharmacy
Kevin Matha, Brice Calvignac, Jean-Pierre Gangneux, Jean-Pierre Benoit
Summary: Visceral leishmaniasis is a neglected disease in many countries, but nanomedicine has shown success in treating it, particularly with liposomal amphotericin B's remarkable cure rates. However, developing more efficient strategies like targeting functionalization, oral formulations, or combined therapies face numerous obstacles and raise many questions.
EXPERT OPINION ON DRUG DELIVERY
(2021)
Review
Pharmacology & Pharmacy
Melody Riaud, M. Carmen Martinez, Claudia N. Montero-Menei
Article
Materials Science, Biomaterials
Assia Rmaidi, Mischa Zelzer, Laurence Sindji, Raphael Dima, Frank Boury, Nicolas Delorme, Claudia N. Montero-Menei
Summary: This study aimed to characterize the physicochemical properties of two polymeric microsphere (MS) systems and evaluate the effect of laminin and poly-n-lysine coatings on stem cell behavior. The results showed that PLGA microspheres promoted protein adsorption and cell adhesion, while PLGA-P188-PLGA microspheres had less protein adsorption and resulted in round cell morphology. However, the long-term cell behavior, including cell number, differentiation profile, and ECM secretion, were not significantly influenced by the type of microcarrier used. The study suggests that the ECM molecules surrounding the microcarrier/cell constructs play a role in converging cell behavior over time.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.