Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Cell & Tissue Engineering
Zhicheng Xu, Qun Zhang
Summary: This study investigated the feasibility of engineering large vessels using ASC sheets and PGA fibers, showing promising potential for constructing large vessel walls.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Brandan Walters, Paul A. Turner, Bernd Rolauffs, Melanie L. Hart, Jan P. Stegemann
Summary: The combination of GF release from microspheres and cyclic stretch enhanced ASCs differentiation, promoting their transformation into SMCs, as confirmed by protein expression and cell morphology data.
Article
Biotechnology & Applied Microbiology
Rowoon Park, Jung Won Yoon, Jin-Ho Lee, Suck Won Hong, Jae Ho Kim
Summary: Researchers have developed an ultrathin cell-culture platform with potential-responsive nanoscale biointerfaces for monitoring mesenchymal stem cells (MSCs). The platform allows for highly ordered organization and spontaneous differentiation of MSCs, providing a robust strategy for directing stem cells and differentiation.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Federica Grilli, Ennio Albanesi, Beatriz Pelacho, Felipe Prosper, Paolo Decuzzi, Daniele Di Mascolo
Summary: Depositing stem cells at the injury site is a clinically relevant strategy for tissue repair and angiogenesis, but insufficient cell engraftment and survival requires the development of new scaffolds. In this study, a microstructured poly(lactic-co-glycolic acid) (PLGA) fabric was investigated as a promising biodegradable scaffold for adipose-derived stem cell (ADSC) tissue integration. The results showed that ADSCs reassembled into spheroidal-like structures on the PLGA fabric, preserving cell viability and promoting a nonlinear actin organization. Moreover, the secretion of specific factors involved in angiogenesis, extracellular matrix remodeling, and stem cell homing was enhanced on the PLGA fabric compared to conventional substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Orthopedics
Xing Guo, Denghua Huang, Dan Li, Longfei Zou, Hui Lv, Yuanhui Wang, Meiyun Tan
Summary: This study found that hypoxia preconditioning attenuates the proliferation and migration ability of ADSCs, but has positive impact on tenogenic differentiation through HIF-1 alpha signaling pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Medicine, General & Internal
Minsung Kim, Bo-Young Oh, Ji-Seon Lee, Dogeon Yoon, You-Rin Kim, Wook Chun, Jong Wan Kim, Il Tae Son
Summary: This study demonstrated for the first time that implanted adipose-derived stem cells can restore impaired smooth muscle cells at the injury site, showing stem cell fate corresponding to the established IAS-specific AI model.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Cell & Tissue Engineering
Souzan Salemi, Jenny A. Prange, Valentin Baumgartner, Deana Mohr-Haralampieva, Daniel Eberli
Summary: Tissue engineering has seen significant advancements in recent years, particularly in the application of stem cells for skeletal and smooth muscle cell engineering. The use of adult stem cells for muscle engineering shows promise, but still faces challenges in selecting the appropriate cell type and controlling differentiation and proliferation.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Engineering, Biomedical
Tong Liu, Jia Xu, Xun Pan, Zhangfan Ding, Hao Xie, Xiaoyi Wang, Huixu Xie
Summary: Autologous tissue transplantation is an effective method for restoring oral and maxillofacial functions and aesthetics, but faces limitations such as complications from donor sites, limited tissue volume, and uncertain long-term outcomes. Adipose-derived mesenchymal stem cells (ADMSCs) have multilineage differentiation capabilities and are important for oral and maxillofacial tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Moema de Alencar Hausen, Ariana de Souza Moraes, Flavia Pedrini, Felipe Grabarz, Jose Angelo Camilli, Eliana Aparecida de Rezende Duek
Summary: This study introduces a natural biomaterial device that can improve the wound healing process by modulating macrophage behavior in vitro. The device, assembled with adipose-derived stem cells and biocompatible polymers, promotes the release of anti-inflammatory cytokines, indicating the prevalence of M2 macrophages.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Cell & Tissue Engineering
Nicolo C. Brembilla, Hubert Vuagnat, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: Chronic wounds have a significant impact on quality of life and healthcare costs. Adipose-derived stromal cells (ASC) have emerged as a promising therapy for chronic wounds. Studies have shown the clinical efficacy of ASC-based therapies in treating chronic wounds.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Lijun Fang, Jingyi Mei, Boqian Yao, Jiang Liu, Peng Liu, Xichun Wang, Jiahui Zhou, Zhanyi Lin
Summary: Tissue-engineered blood vessels (TEBVs) have potential as a therapeutic option for replacing diseased blood vessels. This study investigates the use of induced pluripotent stem cell-derived vascular smooth muscle cells (hiPSC-VSMCs) as seed cells for TEBV construction. The researchers found that hypoxia treatment during differentiation enhanced the formation, adhesion, and amplification rates of hiPSC-VSMCs. Hypoxic conditions also increased cell viability and promoted the secretion of collagen I and elastin. These findings suggest that hypoxic treatment can effectively induce proliferative hiPSC-VSMCs, expanding the sources of high-quality seed cells for TEBV development.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mei-Ling Ho, Chin-Jung Hsu, Che-Wei Wu, Ling-Hua Chang, Jhen-Wei Chen, Chung-Hwan Chen, Kui-Chou Huang, Je-Ken Chang, Shun-Cheng Wu, Pei-Lin Shao
Summary: This study investigated the effect of adipose-derived stem cell (ADSC)-secreted extracellular vesicles (EVs) on osteoblast function. The results showed that ADSC-EVs can enhance osteoblast proliferation and activity, and may regulate osteoblast function through autophagy or signaling pathways.
Article
Engineering, Biomedical
Jiesi Luo, Yuyao Lin, Xiangyu Shi, Guangxin Li, Mehmet H. Kural, Christopher W. Anderson, Matthew W. Ellis, Muhammad Riaz, George Tellides, Laura E. Niklason, Yibing Qyang
Summary: Research successfully derived functional xenogeneic-free hiPSC-VSMCs by replacing animal-derived reagents with functional counterparts of human origin, and developed tissue rings with comparable mechanical strength. Engineered vascular tissues were generated by seeding XF-hiPSC-VSMCs onto biodegradable PGA scaffolds, suitable for implantation into an immunodeficient mice model.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.