Article
Biochemistry & Molecular Biology
Praphawi Nattasit, Kunimichi Niibe, Masahiro Yamada, Yumi Ohori-Morita, Phoonsuk Limraksasin, Watcharaphol Tiskratok, Masaya Yamamoto, Hiroshi Egusa
Summary: The stiffness of the substrate can regulate the behavior and differentiation of induced pluripotent stem cell (iPSC)-derived embryoid bodies (EB). A 3D hydrogel-sandwich culture (HGSC) system was developed to investigate the effects of mechanical cues on iPSC-EB differentiation. The moderate-stiffness HGSC promotes ectoderm and mesoderm lineage differentiation, as well as cardiomyocyte differentiation and myofibril structural maturation.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Xiang Zhao, Bethany N. Radford, Mark Ungrin, Wendy Dean, Myriam Hemberger
Summary: Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri-gastrulation stage conceptus. Trophoblast cells of the extraembryonic ectoderm play a role in delaying cardiomyocyte differentiation dynamics and prolonging pluripotency in embryonic stem cells (ESCs). These findings provide insights into the intricate crosstalk between different compartments of the early conceptus and have implications for synthetic embryo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Olga Gordeeva, Andrey Gordeev, Pavel Erokhov
Summary: The study developed a 3D embryoid body (EB) differentiation model to investigate the relationship between EB architecture formation, patterning, and scaling with cell numbers, external environmental factors, cellular positional information, and culture media factor content. The findings suggest that EB patterning strongly depends on cellular positional information and the content of culture media factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shen Wang, Jun Zhang, Xiang Wu, Xianrong Lin, Xiao-Min Liu, Jun Zhou
Summary: N-6-methyladenosine (m(6)A) regulates physiological processes including stem cell differentiation. YTHDF1 and YTHDF3 play different roles in controlling embryonic stem cell differentiation, with YTHDF3 depletion leading to loss of pluripotency and YTHDF1 depletion impairing cardiomyocytes differentiation.
Article
Cell & Tissue Engineering
Karin Lauschke, Luca Volpini, Yong Liu, Anne Marie Vinggaard, Vanessa Jane Hall
Summary: The study compared the expression of heart-isl1NKX2.5 cardiomyogenesis markers between pig and human induced pluripotent stem cell differentiation processes, and found differences between the pig and mouse models in terms of marker expression during embryonic development. The study suggests using FLK1/PDGFR-alpha for identifying cardiac mesoderm and ISL1/NKX2.5 for cardiac progenitors in future differentiation studies.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Developmental Biology
Sara A. Miller, Manashree Damle, Jongmin Kim, Robert E. Kingston
Summary: The study showed that full methylation of H3K27 was not necessary for the formation of differentiated cell states during embryoid body formation, but was required to maintain a stable differentiated state.
Article
Engineering, Environmental
Xiaoxing Liang, Renjun Yang, Nuoya Yin, Francesco Faiola
Summary: It was found that substitutes for BPA may have adverse effects on lineage commitment and lipid metabolism during early embryonic development, with BPE showing the mildest impact among the seven bisphenols analyzed.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Multidisciplinary Sciences
Katharine Goodwin, Jacob M. Jaslove, Hirotaka Tao, Min Zhu, Sevan Hopyan, Celeste M. Nelson
Summary: By using single-cell RNA-sequencing of embryonic mouse lungs, this study reveals that the pulmonary mesenchyme contains a continuum of cell identities, but no transcriptionally distinct progenitors. Transcriptional variability is correlated with spatially distinct sub-epithelial and sub-mesothelial mesenchymal compartments that are regulated by Wnt signaling. Live-imaging and tension-sensors show that sub-epithelial mesenchyme contributes to airway smooth muscle. Reconstruction of differentiation trajectories reveals early activation of cytoskeletal and Wnt signaling genes.
Article
Biochemistry & Molecular Biology
Lu Qiao, So Hee Dho, Ji Young Kim, Lark Kyun Kim
Summary: SEPHS1 plays a minor role in maintaining pluripotency in mouse embryonic stem cells (ESCs), but is crucial for germ layer differentiation, especially for functional cardiac lineage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Duo Wang, Chang Liu, Huan Liu, Yilei Meng, Fang Lin, Yanqiong Gu, Hanrui Wang, Mengyue Shang, Chang Tong, Agapios Sachinidis, Qilong Ying, Li Li, Luying Peng
Summary: The interaction between ERG1 and Integrin beta 1 in rESCs activates the AKT pathway, leading to cardiomyocyte differentiation through multiple mechanisms including upregulation of beta-catenin and Gata4 expression and inhibition of cell apoptosis via nuclear translocation of nuclear factor-kappa B.
Editorial Material
Biochemistry & Molecular Biology
Natalia Y. Martynova, Elena A. Parshina, Andrey G. Zaraisky
Summary: Zyxin, initially recognized as a minor actin cytoskeleton protein, not only regulates actin filaments assembly and repair but also plays a crucial role in gene expression and cell differentiation, particularly during embryogenesis process.
Review
Chemistry, Multidisciplinary
Se Young Park, Seongjae Lee, Jeehye Yang, Moon Sung Kang
Summary: Pixelating patterns of red, green, and blue quantum dots is a critical challenge for high-end displays in virtual, augmented, and mixed reality. Photopatterning based on light-induced chemical conversion of QD films is a promising method for forming precise micrometer-scale QD patterns that can be commercially viable. This article reviews recent progress in QD patterning using photolithography, including different methods and high-resolution achievements, and discusses future research directions.
ADVANCED MATERIALS
(2023)
Article
Biochemical Research Methods
Yu-Ting Ti, Hsiao-Chi Cheng, Ying Li, Hsiung-Lin Tu
Summary: The study introduces a strategy to fabricate highly multiplexed supported lipid bilayer (SLB) and protein arrays using a reusable microfluidic chip. Lipid mobility is crucial, and extending the accessibility range of mobility was achieved by adding protein rings as diffusion barriers.
Review
Biochemical Research Methods
Jin Zhang, Jing Xue, Ningfeng Luo, Feng Chen, Badong Chen, Yongxi Zhao
Summary: Single-cell profiling is crucial for understanding cellular heterogeneity and cell fate. Microfluidics has emerged as an optimal tool for single-cell profiling due to its high throughput and automation advantages. Among different microfluidic platforms, microwell array chips offer simplicity and easy integration with in situ analysis, making them ideal for single-cell studies. This review summarizes recent advances in single-cell analysis based on microwell array chips, including chip design and preparation, cell capture and lysis strategies, as well as advanced analysis of single-cell proteins, nucleic acids, and metabolites. The challenges and opportunities in the development of microwell-based single-cell analysis are also discussed.
Article
Chemistry, Multidisciplinary
Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano
Article
Chemistry, Multidisciplinary
Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano
Article
Materials Science, Biomaterials
Young-Jin Kim, Seiichiro Yamamoto, Haruko Takahashi, Naruo Sasaki, Yukiko T. Matsunaga
BIOMATERIALS SCIENCE
(2016)
Correction
Materials Science, Biomaterials
A. Tan, K. Fujisawa, Y. Yukawa, Y. T. Matsunaga
BIOMATERIALS SCIENCE
(2016)
Article
Materials Science, Biomaterials
Young-Jin Kim, Soo Hyeon Kim, Teruo Fujii, Yukiko T. Matsunaga
BIOMATERIALS SCIENCE
(2016)
Article
Materials Science, Biomaterials
Young-Jin Kim, Misa Tachibana, Mitsuo Umezu, Yukiko T. Matsunaga
JOURNAL OF MATERIALS CHEMISTRY B
(2016)
Article
Cell & Tissue Engineering
Yoshinori Arisaka, Jun Kobayashi, Kazuo Ohashi, Kohei Tatsumi, Kyungsook Kim, Yoshikatsu Akiyama, Masayuki Yamato, Teruo Okano
REGENERATIVE THERAPY
(2016)
Review
Engineering, Biomedical
Jun Kobayashi, Masayuki Yamato, Teruo Okano
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2017)
Article
Biochemistry & Molecular Biology
Jun Kobayashi, Yoshinori Arisaka, Nobuhiko Yui, Yoshikatsu Akiyama, Masayuki Yamato, Teruo Okano
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Multidisciplinary Sciences
Haruko Takahashi, Keisuke Kato, Kenji Ueyama, Masayoshi Kobayashi, Gunwoong Baik, Yasuhiro Yukawa, Jun-ichi Suehiro, Yukiko T. Matsunaga
SCIENTIFIC REPORTS
(2017)
Review
Engineering, Biomedical
Jun Kobayashi, Akihiko Kikuchi, Takao Aoyagi, Teruo Okano
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2019)
Review
Chemistry, Multidisciplinary
Jun Kobayashi, Teruo Okano
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2019)
Article
Cell & Tissue Engineering
Jun Kobayashi, Teruo Okano
Summary: Hepatocyte transplantation has been used for the treatment of congenital metabolic liver diseases and liver failure support. Hepatocyte sheet technology using PIPAAm-grafted dishes provides an efficient method for cell transplantation. Shortening the medium depth can maintain hepatocyte monolayer and improve oxygen supply to the cells. The hepatocyte sheets can be easily detached from the dishes and transferred to target sites.
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Chemistry, Multidisciplinary
Jun Kobayashi, Yoshinori Arisaka, Nobuhiko Yui, Masayuki Yamato, Teruo Okano
Summary: The study showed that the heparin-modified PIPAAm surface can maintain the activity of HB-EGF, allowing it to interact with hepatocytes through specific affinity interaction with EGFR. The hydration and swelling of grafted PIPAAm chains on the surface were found to attenuate the attachment of hepatocytes to HB-EGF bound to heparin-IC1.
Proceedings Paper
Computer Science, Artificial Intelligence
Jun Kobayashi, Yoshikatsu Akiyama, Masayuki Yamato, Teruo Okano
2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)
(2016)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.