Article
Engineering, Biomedical
Miji Yeo, Jung Won Yoon, Gyu Tae Park, Sung-Chan Shin, Young-Cheol Song, Yong-Il Cheon, Byung-Joo Lee, Geun Hyung Kim, Jae Ho Kim
Summary: In this study, cell electrospinning (CE) was used to reconstruct esophageal tissues, resulting in uniaxially arranged and elongated smooth muscle cells (SMCs) with upregulated expression levels of SMC-specific markers. Transplantation of SMC-laden nanofibrous patches promoted regeneration of esophageal wounds with increased blood vessels and enhanced expression of SMC-specific markers. CE can be an efficient strategy to reconstruct smooth muscle tissues and treat esophageal perforation, providing guidance for cell morphology and accelerated SMC differentiation.
MATERIALS TODAY BIO
(2023)
Review
Chemistry, Multidisciplinary
Darshan Tagadur Govindaraju, Chih-Hao Chen, K. T. Shalumon, Hao-Hsi Kao, Jyh-Ping Chen
Summary: The limited natural healing capacity of tendons and ligaments presents a challenge for effective therapeutic strategies. Tissue engineering, using biomaterials, cells, and biochemical signals, has shown promising results in restoring the physiological functions of these tissues. This paper reviews the structure and healing mechanisms of tendons and ligaments, and discusses the use of bioactive nanostructured scaffolds, with a focus on electrospun fibrous scaffolds, in tissue engineering.
Review
Developmental Biology
Michael Donadon, Massimo M. Santoro
Summary: The article primarily describes the developmental origin of smooth muscle cells in different organs, as well as their specification and differentiation in the cardiovascular, respiratory, and intestinal systems. It discusses the important roles of smooth muscle in organ development and how they instruct the development of other organs through signaling and mechanical feedback mechanisms. By understanding smooth muscle cell development, the aim is to advance therapeutic approaches related to tissue regeneration and other smooth muscle-related diseases.
Article
Engineering, Environmental
K. T. Shalumon, Han-Tsung Liao, Wei-Hao Lin, T. G. Darshan, P. A. Mini, Jyh-Ping Chen
Summary: In this study, a combination approach using a tendon tissue engineering scaffold with chemical and physical cues, including fiber alignment, growth factor and mechanical stimulation, demonstrated successful tendon regeneration and repair.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Serafina Pacilio, Roberta Costa, Valentina Papa, Maria Teresa Rodia, Carlo Gotti, Giorgia Pagnotta, Giovanna Cenacchi, Maria Letizia Focarete
Summary: Tissue engineering utilizes a scaffold, cells, and regulatory signals to recreate a biomimetic extracellular matrix that supports cell attachment and proliferation. This study focused on the role of an electrospun scaffold made of a biocompatible polymer in skeletal muscle myogenesis, aiming to provide an alternative approach to tissue regeneration. The functionalized scaffold successfully reproduced the nanostructure of skeletal muscle and guided myoblast morphogenesis, promoting cell differentiation and tissue formation. Furthermore, it enhanced myoblast differentiation within a shorter timeframe compared to traditional 2D cell culture techniques.
BIOENGINEERING-BASEL
(2023)
Review
Cell & Tissue Engineering
Souzan Salemi, Jenny A. Prange, Valentin Baumgartner, Deana Mohr-Haralampieva, Daniel Eberli
Summary: Tissue engineering has seen significant advancements in recent years, particularly in the application of stem cells for skeletal and smooth muscle cell engineering. The use of adult stem cells for muscle engineering shows promise, but still faces challenges in selecting the appropriate cell type and controlling differentiation and proliferation.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Multidisciplinary Sciences
Nicholas Rogozinski, Apuleyo Yanez, Rahulkumar Bhoi, Moo-Yeal Lee, Huaxiao Yang
Summary: 3D cardiac engineered constructs not only advance cardiac regenerative medicine but also enable more accurate modeling of healthy and diseased cardiac tissues. This review highlights the relationship between different cell types and advanced fabrication methods, shedding light on various pathologies and providing therapeutic potential for damaged myocardium. The future direction is focused on creating specialized and personalized constructs with region-specific microtopography and function.
Article
Polymer Science
Meng Wang, Shigang Lin, Kibret Mequanint
Summary: This study evaluated the potential of using synthesized polymers and electrospun scaffolds to enhance differentiation of iPSC-derived mesenchymal stem cells into smooth muscle cells, providing a feasible strategy for engineering functional vascular tissues.
Article
Polymer Science
Shima Majidansari, Negin Vahedi, Mahmood Rekabgardan, Camellia Ganjoury, Najmeh Najmoddin, Mohammad Tabatabaei, Faraz Sigaroodi, Paniz Naraghi-Bagherpour, Seyed Amir Ali Taheri, Mohammad-Mehdi Khani
Summary: Cardiovascular diseases are a major cause of death worldwide, and tissue engineering offers promising strategies for their treatment. This study demonstrates that a nanofibrous scaffold produced through electrospinning can support and significantly promote endothelial differentiation of mesenchymal stem cells.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2022)
Article
Materials Science, Multidisciplinary
Jianing Yan, Xixia Liu, Jingyi Liu, Xinjie Zhang, Qiang Zheng, Junjie Nan, Mengjia Lin, Haoqi Pan, Yifan Wang, Xiujun Cai, Jun Yin
Summary: A dual-layer tubular scaffold was fabricated to support bile duct regeneration, providing adequate mechanical strength and biocompatibility, showing superior results in animal models.
MATERIALS & DESIGN
(2021)
Article
Cell Biology
Ravi Abishek Bharadhwaj, Regalla Kumarswamy
Summary: This study reveals a novel role for the lncRNA TUG1 in modulating SMC differentiation via the KLF4-myocardin axis, which may have potential implications in AAA development.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Srikanth Sivaraman, Jackson Hedrick, Samia Ismail, Chris Slavin, Raj R. Rao
Summary: Research focused on evaluating the efficacy of different growth factors combinations and culture periods in MSC differentiation into SMCs, and was validated through RNA sequencing. Results showed that differentiation in PDGF + TGF beta 1-infused medium significantly increased SMC markers and decreased MSC markers, enhancing vascular smooth muscle formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Maurice N. Collins, Guang Ren, Kieran Young, S. Pina, Rui L. Reis, J. Miguel Oliveira
Summary: Bone tissue engineering aims to create biofunctional tissue for treating diseases, with scaffold fabrication techniques and biomaterial selection being crucial. Vascularization and immunomodulation are major challenges in this field.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell Biology
Guo Chen, Shuang Chen, Xingpeng Di, Shengyin He, Yugao Liu, Rui Qu, Yi Luo, Yuebai Liu, Luo Yang
Summary: This study found that upregulation of survivin promotes bladder remodeling caused by BOO and plays a role through cell proliferation, fibrotic protein expression, and autophagy. Inhibiting survivin may be a potential therapeutic approach for treating BOO-related bladder remodeling.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alvaro Yogi, Marina Rukhlova, Claudie Charlebois, Ganghong Tian, Danica B. Stanimirovic, Maria J. Moreno
Summary: The study found that VSMCs differentiated from adipose-derived stem cells (ASCs) show potential for populating PET-based vascular scaffolds. By shortening the differentiation period and studying the role of downstream effectors of TGF-beta, the process was stabilized. The non-woven PET structure did not affect ASC differentiation, providing a novel alternative to address issues with long-term patency of vascular grafts.
Article
Urology & Nephrology
Simon C. Baker, Andrew S. Mason, Jennifer Southgate
Meeting Abstract
Oncology
S. C. Baker, A. S. Mason, J. Southgate
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2020)
Review
Biochemistry & Molecular Biology
Smarakan Sneha, Simon C. Baker, Andrew Green, Sarah Storr, Radhika Aiyappa, Stewart Martin, Klaus Pors
Summary: Advances in treatment strategies for breast cancer face challenges in achieving selective treatment with limited side effects; The CYP family of enzymes play important roles in cancer cell proliferation, cell signaling, and drug metabolism, impacting treatment outcomes; Understanding CYP expression and its role in metabolizing steroid hormones and xenobiotics is crucial for the pathogenesis of breast cancer.
Article
Engineering, Biomedical
Ashley Ward, Debora Morgante, John Fisher, Eileen Ingham, Jennifer Southgate
Summary: Bladder acellular matrix has potential applications in reconstructive surgeries, but the decellularization process is complex. This study used a mechanical engineering approach to design a laser-printed flat-bed apparatus for reproducible and full-thickness bladder tissue decellularization.
BIOMEDICAL MATERIALS
(2021)
Article
Biochemical Research Methods
Chung-Yao Wang, Edmund Bergstrom, Jennifer Southgate, Jane Thomas-Oates
Summary: This study aimed to investigate the highly differentiated urothelial apical surface glycome. A method was developed to collect tissue apical surface N- and O-glycans to characterize the glycome. The findings are important for understanding urothelial differentiation and the development of urothelial diseases.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Simon C. Baker, Andrew S. Mason, Raphael G. Slip, Katie T. Skinner, Andrew Macdonald, Omar Masood, Reuben S. Harris, Tim R. Fenton, Manikandan Periyasamy, Simak Ali, Jennifer Southgate
Summary: Limited understanding of bladder cancer aetiopathology hampers progress in reducing incidence. This study provides missing mechanistic evidence linking reactivated BKPyV-infection to bladder cancer risk.
Article
Pathology
Jennifer Hinley, Rosalind Duke, Jessica Jinks, Jens Stahlschmidt, David Keene, Raimondo M. Cervellione, Imran Mushtaq, Paolo De Coppi, Massimo Garriboli, Jennifer Southgate
Summary: Bladder exstrophy (BEX) is a rare developmental abnormality that results in an open bladder plate. This study found that patient-derived BEX epithelial cells in vitro can form a barrier-forming epithelium under specific conditions. The expression of squamous KRT14 transcript is inversely related to barrier development, and transcriptional drivers of urothelial differentiation show reduced expression in squamous BEX cultures.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andrew S. Mason, Claire L. Varley, Olivia M. Foody, Xiang Li, Katie Skinner, Dawn Walker, Tony R. Larson, Daisuke Wakamatsu, Simon C. Baker, Jennifer Southgate
Summary: Urothelium is a specialized epithelium that lines the lower urinary tract and plays a crucial role in maintaining barrier function. This study explored the role of glycerophospholipids in urothelial barrier function and demonstrated the importance of lipid mediators in tissue homeostasis. Transcriptomic analysis revealed metabolic changes during urothelial differentiation, and knocking down the LPCAT4 gene significantly affected cell proliferation and barrier function recovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Simon C. Baker, Andrew S. Mason, Raphael G. Slip, Pontus Eriksson, Gottfrid Sjodahl, Ludwik K. Trejdosiewicz, Jennifer Southgate
Summary: This study found that using interferon gamma (IFN gamma) in the treatment of bladder cancer can improve patients' survival rate, while treatments targeting immune checkpoints have limited effectiveness. The research also discovered that IFN gamma can regulate the expression of immune inhibitory genes, providing new therapeutic targets to enhance immunotherapy.
Letter
Urology & Nephrology
Simon C. Baker, Andrew S. Mason, Jennifer Southgate
Article
Cell & Tissue Engineering
Debora Morgante, Anna Radford, Syed K. Abbas, Eileen Ingham, Ramnath Subramaniam, Jennifer Southgate
Summary: This study demonstrates the anti-inflammatory and tissue-integrative properties of non-crosslinked acellular matrices, providing evidence for their incorporation into surgical procedures, such as in primary complex hypospadias repair.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.