Article
Chemistry, Analytical
Dandan Jiang, Siyu Wu, Siqi Lv, Ruixue Qi, Yangyang Li, Jinghai Liu
Summary: A new chelator, L-Alanyl-L-Glutamine, was used to prepare immobilized magnetic graphite nitride material with cerium ions. The resulting nanocomposite showed high surface area, hydrophilicity, positive electricity, and magnetic response. As an immobilized metal ion affinity chromatography sorbent, it exhibited high sensitivity, selectivity, and recyclability. The analysis of A549 cell lysate identified numerous phosphopeptides mapped to phosphoproteins.
Article
Chemistry, Applied
Wenrui Wu, Ruizhi Tang, Zhouxian Li, Yehua Shen, Shujuan Ma, Junjie Ou
Summary: A Ti4+-immobilized polydispersed phosphate-rich microsphere was successfully fabricated using inverse suspension polymerization method, showing excellent sensitivity, selectivity, and adsorption capacity. This method requires only two steps to synthesize Ti4+-IMAC, offering a simpler and more efficient approach for protein phosphorylation research and potential for pilot production and commercialization.
Article
Engineering, Chemical
Wei Gao, Feng Zhang, Sen Zhang, Jia-yuan Li, Hong-zhen Lian
Summary: High selective enrichment of low abundance phosphopeptides from complex biological samples is essential for in-depth phosphoproteomics. A magnetic material called mMOF-FBP-Ti4+, fabricated by grafting UiO-66-NH2 metal-organic framework onto the surface of Fe3O4 and immobilizing Ti(IV) with fructose 1,6-bisphosphate, exhibited favorable properties for phosphopeptide enrichment. The material demonstrated strong affinities through metal oxide affinity chromatography and immobilized metal affinity chromatography, as well as improved hydrophilicity for enhanced hydrophilic chromatography interaction. The mMOF-FBP-Ti4+ also yielded low detection limit, high selectivity, good reusability, and high postenrichment recovery towards phosphopeptides, making it a promising tool for efficient enrichment in complex biological samples.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Kexuan Wang, Ajuan Yu, Yu Gao, Miao Chen, Hang Yuan, Shusheng Zhang, Gangfeng Ouyang
Summary: A novel immobilized metal affinity chromatography (IMAC) functional composite, mNi@N-GrT@PDA@Ti4+, was fabricated for selective enrichment and mass spectrometry identification of phosphorylated peptides. The composite showed high specificity and low detection limits in the enrichment of phosphopeptides from protein digest mixtures. It also demonstrated successful selective enrichment of phosphopeptides in complex biological samples.
Article
Biochemical Research Methods
Juan Li, Xuefang Dong, Yun Cui, Siyang Li, Cheng Chen, Xiaofei Zhang, Xiuling Li, Xinmiao Liang, Ying Zhu
Summary: Protein phosphorylation and O-linked glycosylation are critical posttranslational modifications that target identical amino acid residues and generate crosstalk. This study established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides, providing a valuable tool for analyzing the crosstalk between these two modifications.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Hongwei Wang, Ruizhi Tang, Shicong Jia, Shujuan Ma, Bolin Gong, Junjie Ou
Summary: This paper introduces a novel monodisperse MAR microsphere (Ti4+-Brush@MAR) prepared and modified via surface-initiated atom transfer radical polymerization (SI-ATRP), showing high enrichment specificity for phosphopeptides in beta-casein or milk digest samples.
Article
Chemistry, Analytical
Bin Liu, Baichun Wang, Yinghua Yan, Keqi Tang, Chuan-Fan Ding
Summary: The Fe3O4@mSiO(2)@APA@Ti4+/Nb5+ material has shown promising potential in effectively enriching global phosphopeptides, with high selectivity, low detection limit, good reproducibility, and excellent loading capacity.
Article
Chemistry, Multidisciplinary
Shujuan Ma, Ruizhi Tang, Yang Yu, Luwei Zhang, Haiyang Zhang, Wenyong Ding, Junjie Ou
Summary: A novel Ti4+-immobilized microsphere was developed as a reusable IMAC sorbent, demonstrating excellent enrichment capability and prolonged lifespan. Its superior performance in phosphopeptide enrichment suggests promising prospects for future commercialization.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Biochemical Research Methods
Tomoto Ura, Tomoshi Kameda, Fina A. Laksmi, Matsujiro Ishibashi, Tsutomu Arakawa, Kentaro Shiraki, Atsushi Hirano
Summary: Immobilized metal ion affinity chromatography (IMAC) is a useful method for purifying histidine-tagged or histidine-rich proteins and peptides. However, phenolic compounds interfere with IMAC purification of proteins from plant-based host systems. This study investigated the affinity of phenolic compounds for transition metal ions and found that the more hydroxyl groups a compound has, the higher its affinity for transition metal ions. Importantly, phenolic compounds showed no obvious affinity for the Ni(II)-IMAC column, while imidazole and histidine-tagged proteins demonstrated binding to the Ni(II)-IMAC column.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Ruizhi Tang, Yang Yu, Jing Dong, Yating Yao, Shujuan Ma, Junjie Ou, Mingliang Ye
Summary: A simple one-step modification strategy was developed to prepare bifunctional adsorbents for HILIC and IMAC, showing excellent enrichment efficiency in glycopeptides and phosphopeptides identification from HeLa cell proteins. These materials have demonstrated feasibility for real sample applications and potential business value by successfully analyzing multiple biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Xiaoya Zhang, Quanshou Feng, Zehu Xie, Fuxing Xu, Yinghua Yan, Chuanfan Ding
Summary: In this study, a new type of covalent organic framework material was successfully prepared for the enrichment of phosphorylated peptides and exosomes using an immobilized metal ion affinity chromatography strategy. The material showed high selectivity and sensitivity in practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yu Wang, Yini Pan, Zhichao Yan, Zhihua Zhong, Lingyi Zhang, Weibing Zhang
Summary: Researchers designed and synthesized two types of magnetic resin composites and evaluated their applications for enriching proteins, peptides, and phosphopeptides. The composites showed higher adsorption capacities and efficient enrichment of proteins, peptides, and phosphopeptides. This study reveals the potential of magnetic resin composites as enrichment materials for proteomics and phosphoproteomics analysis.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Jacob H. Forstater, Shane T. Grosser
Summary: Immobilization technology plays a significant role in commodity chemical synthesis and pharmaceutical synthesis. This study presents a data-rich approach that automates the screening and development of affinity-based enzyme immobilizations, enabling real-time process characterization and rapid decision making. The developed methodology reduces the time and resources required for immobilization process development and optimization.
REACTION CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Analytical
Dandan Jiang, Siqi Lv, Xue Han, Limei Duan, Jinghai Liu
Summary: Exfoliated magadiite nanosheets embedded with Fe3O4 were utilized to prepare Gd3+-immobilized magnetic magadiite nanosheets as an adsorbent for phosphopeptides enrichment. The 2D morphology of the adsorbent provided high surface area and abundant Gd3+ contents, leading to efficient phosphopeptides capture with low detection limit and stable enrichment performance. Further evaluation demonstrated the capability of the nanocomposite to enrich phosphopeptides from various biological samples.
Article
Nanoscience & Nanotechnology
Sen Zhang, Jia-yuan Li, Wei Gao, Jun-qin Qiao, Hong-zhen Lian
Summary: Ti3C2-Fe3O4 composites with exposed non-modified Ti3C2 MXene nanosheets were designed and prepared for the enrichment of phosphopeptides. The composites interacted with the phosphopeptides through metal oxide affinity chromatography and hydrophilic interaction chromatography mechanisms. The magnetic nanomaterial showed high sensitivity and selectivity, and satisfactory results were obtained in the enrichment of phosphopeptides from real samples and traditional Chinese medicine injection.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)