Article
Food Science & Technology
Manyun Yang, Xiaobo Liu, Yaguang Luo, Arne J. Pearlstein, Shilong Wang, Hayden Dillow, Kevin Reed, Zhen Jia, Arnav Sharma, Bin Zhou, Dan Pearlstein, Hengyong Yu, Boce Zhang
Summary: The system developed integrates paper chromogenic arrays (PCAs) and machine learning to automatically recognize PCA patterns on multiplexed viable pathogens with strain-level specificity. This approach has the potential to advance non-destructive pathogen detection and identification on food.
Review
Food Science & Technology
Qingying Chen, Xingguo Zhang, Qingqing Wang, Jingxian Yang, Qingping Zhong
Summary: Listeria monocytogenes is a significant foodborne pathogen that forms biofilms on food and food contact surfaces, leading to equipment damage, food spoilage, and human diseases. Mixed biofilms, including those formed by L. monocytogenes and other bacteria, exhibit higher resistance to disinfectants and antibiotics. The structure and interspecific interaction of mixed biofilms are complex, and their role in the food industry remains to be explored.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Food Science & Technology
Emily Forauer, Sophie Tongyu Wu, Andrea J. Etter
Summary: Listeria monocytogenes is a significant concern in retail delis, especially in sliced meat products; the presence of the pathogen in deli environments is influenced by factors such as sanitizer tolerance and biofilm formation. Daily intervention and deep cleaning, along with employee commitment to cleaning programs and a strong food safety culture, are important strategies for improving retail deli cleanliness and reducing the prevalence of L. monocytogenes.
Article
Multidisciplinary Sciences
Bryan A. Ramirez-Corona, Anna C. Love, Srikiran Chandrasekaran, Jennifer A. Prescher, Zeba Wunderlich
Summary: This article introduces a non-invasive bioluminescent imaging strategy that allows real-time observation of pathogen load changes in individual fruit flies. By using luminescent bacteria for monitoring, stochastic responses to infection can be studied over time in individuals, and the dynamics of microbial clearance can be visualized with high temporal resolution.
Article
Biotechnology & Applied Microbiology
Jessika Nowak, Sandra B. Visnovsky, Andrew R. Pitman, Cristina D. Cruz, Jon Palmer, Graham C. Fletcher, Steve Flint
Summary: Listeria monocytogenes is a common foodborne pathogen that contaminates food during processing, likely due to biofilm formation. Multiple genes have been identified to be involved in biofilm formation by this pathogen, impacting biofilm structure.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria Guadalupe Avila-Novoa, Berenice Gonzalez-Torres, Jean Pierre Gonzalez-Gomez, Pedro Javier Guerrero-Medina, Liliana Martinez-Chavez, Nanci Edid Martinez-Gonzales, Cristobal Chaidez, Melesio Gutierrez-Lomeli
Summary: In this study, the diversity of virulence factors associated with the mechanisms of pathogenicity, persistence, and formation of biofilm in L. monocytogenes were investigated using whole-genome sequencing. The findings contribute to the understanding of the genomic diversity and epidemiology of L. monocytogenes, and provide valuable insights for establishing control measures to reduce its impact on the environment and consumers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Jialin He, Xiangyu Gao, Hanbing Huang, Jianxiong Hao
Summary: This study utilizes confocal laser scanning microscopy (CLSM) and ISA-2 software analysis to quantitate and visualize the structural changes of biofilms. The removal process of Listeria monocytogenes (LM) biofilms using different disinfectants is investigated. The results show that the biofilm gradually thins and changes in morphology, which is consistent with first-level kinetics. The proposed theory of layer-by-layer elimination of LM biofilm is validated.
Article
Microbiology
Benjamin Zwirzitz, Stefanie U. Wetzels, Emmanuel D. Dixon, Svenja Fleischmann, Evelyne Selberherr, Sarah Thalguter, Narciso M. Quijada, Monika Dzieciol, Martin Wagner, Beatrix Stessl
Summary: Foodborne outbreaks related to Listeria monocytogenes are often linked to meat and meat products, with recontamination during meat processing being a major concern. Persistence of L. monocytogenes in multi-species biofilms poses a challenge for cleaning and disinfection measures. Analysis of microbial communities in a meat processing facility revealed widespread Listeria contamination and identified key genera associated with Listeria spp. and L. monocytogenes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Priscilla Sinclair, M. Laura Rolon, Jingzhang Feng, Adrian F. Padin-Lopez, Luke LaBorde, Jasna Kovac
Summary: This study evaluated the antilisterial activity of two LAB strains against L. monocytogenes and found that the inhibitory effects varied depending on the assay method used. The results highlight the importance of considering the complex microbiome in food processing facilities when assessing the potential of LAB strains for biocontrol.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Kye-Hwan Byun, Sang Ha Han, Min Woo Choi, Byoung-Hu Kim, Si Hong Park, Sang -Do Ha
Summary: This study evaluated the biofilm eradication ability of a specific bacteriophage against Listeria monocytogenes biofilms and investigated the gene and structural changes after phage treatment. The results showed that the phage was able to effectively eliminate the biofilms and caused up-regulation of certain genes and changes in structural parameters.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
M. Bermudez-Capdevila, B. R. H. Cervantes-Huaman, J. J. Rodriguez-Jerez, C. Ripolles-Avila
Summary: This study aims to determine the minimum inhibitory concentration (MIC) of four essential oils in four strains of Listeria monocytogenes and investigate the effect of subinhibitory doses of the most effective oil on the strains, as well as their sensitivity to antibiotics after exposure to the oil. The results showed a decrease in the initial MIC after continuous exposure to 1/2 MIC, but no differences were observed in biofilm formation. Additionally, differences were observed in the structure of the biofilms and extracellular matrix before and after contact with the oil.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Veterinary Sciences
Maria-Leonor Lemos, Joana C. Prata, Ines C. Rodrigues, Sofia Martins-Costa, Bernardo Archer, Jorge Machado, Rui Dilao, Paulo Vaz-Pires, Paulo Martins da Costa
Summary: This study focuses on improving the shelf-life and safety of fresh salmon, which is often consumed raw. Good hygiene practices and vacuum packaging extended the shelf-life up to 20 days. The presence of Carnobacterium maltaromaticum inhibited the growth of Listeria monocytogenes, which is responsible for foodborne illness. Seasonings, such as oregano oil, lemon juice, garlic powder, and salt, showed inhibitory effects on spoilage bacteria and L. monocytogenes. The development of a salmon medium showed a good correlation with bacterial growth in a standard commercial culture medium.
VETERINARY SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anne-Sophie Hascoet, Carolina Ripolles-Avila, Brayan R. H. Cervantes-Huaman, Jose Juan Rodriguez-Jerez
Summary: Listeria monocytogenes remains a significant public health challenge in the meat sector, and utilizing Bacillus safensis from resident microbiota as a potential inhibitor could be an effective strategy for controlling the pathogen. The preformed biofilms of Bacillus safensis have shown to inhibit adhesion and biofilm formation of L. monocytogenes strains, providing a new approach to combat the persistence of the pathogen in meat industry surfaces.
Article
Chemistry, Physical
Mayssane Hage, Nour-Eddine Chihib, Marwan Abdallah, Simon Khelissa, Beatrice Crocco, Hikmat Akoum, Fouad Bentiss, Charafeddine Jama
Summary: The study found that functionalizing stainless steel surfaces with nisin, particularly when linked by its amino group, showed efficient antibacterial activity. Immobilizing nisin onto surfaces resulted in a significant reduction in bacterial count, with a tight relation observed between surface topography, nisin conformation, and antibacterial activity.
SURFACES AND INTERFACES
(2021)
Article
Food Science & Technology
T. Mazaheri, B. R. H. Cervantes-Huaman, L. Turitich, C. Ripolles-Avila, J. J. Rodriguez-Jerez
Summary: This study investigated different treatments for removing Listeria monocytogenes biofilms and found that conventional methods were unable to completely remove the biofilms. Enzymatic treatments at 50℃ showed the greatest effectiveness. A combined treatment using a chlorinated alkaline cleaner followed by an enzymatic treatment was found to enhance bacterial dispersal from surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Katharina J. Kreissig, Lisbeth Truelstrup Hansen, Pernille Erland Jensen, Susse Wegeberg, Ole Geertz-Hansen, Jens J. Sloth
Summary: Several Greenland seaweed species have potential as foods or food ingredients, with high contents of beneficial elements, especially macrominerals and trace minerals. Element profiles differ between species and geographical locations, with iodine contents particularly high in macroalgae of the family Laminariaceae.
Article
Environmental Sciences
Jennifer L. Hayward, Yannan Huang, Lisbeth Truelstrup Hansen, Christopher K. Yost, Craig Lake, Rob C. Jamieson
Summary: This study investigated the mechanisms responsible for antimicrobial resistance attenuation in on-site wastewater treatment systems, focusing on lateral flow sand filters. The primary mechanisms identified were degradation and filtration, with attenuation occurring along the entire length of each filter. The results support the use of conservative design guidelines and separation distances for reducing antimicrobial resistance transmission.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Adeyinka O. Ajayi, Benjamin J. Perry, Christopher K. Yost, Rob C. Jamieson, Lisbeth Truelstrup Hansen, Teddie O. Rahube
Summary: The study isolated and sequenced plasmids encoding meropenem resistance from bacterial hosts within Canadian wastewater treatment plants, identifying a multi-resistance plasmid with various antibiotic resistance genes. This discovery could have significant implications for human and veterinary medicine. Extensive research is needed to further understand the fitness trade-offs of plasmids with different types of conjugative transfer and maintenance modules.
CANADIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Food Science & Technology
Tina Beck Hansen, Somaya Abdalas, Iman Al-Hilali, Lisbeth Truelstrup Hansen
Summary: Listeria monocytogenes is a potentially fatal foodborne pathogen that can tolerate high salt concentrations and refrigerated storage. This study aimed to create a model predicting the effect of salt on heat tolerance of L. monocytogenes in meat and seafood products, and found that salt concentration was positively associated with heat tolerance.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Food Science & Technology
Jonas Steenholdt Sorensen, Sanne Kjaerulf Madsen, Claus Heiner Bang-Berthelsen, Lisbeth Truelstrup Hansen
Summary: Fermentation of seaweed by Lb. plantarum is a promising preservation method, but optimization is needed to ensure product safety. Natural fermentation may result in unsafe products, while adding Lb. plantarum can improve product quality.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Environmental Sciences
Kiley Daley, Rob Jamieson, Daniel Rainham, Lisbeth Truelstrup Hansen, Sherilee L. Harper
Summary: The research aims to estimate microbial health risks caused by wastewater effluent exposure in Arctic Canada and evaluate potential mitigation options. The results suggest that wastewater effluent exposures may contribute to gastrointestinal illness in some Arctic communities. Improved treatment and interventions to prevent access to disposal areas are effective strategies for reducing the risks. Well-designed passive systems appear to be the most effective wastewater treatment option for limiting and managing associated microbial health risks in Arctic Canada.
MICROBIAL RISK ANALYSIS
(2022)
Article
Food Science & Technology
Martin Laage Kragh, Louisa Obari, Alyssa Marie Caindec, Hanne Aarslev Jensen, Lisbeth Truelstrup Hansen
Summary: The study shows that hot-air drying significantly reduces the presence of Salmonella and Listeria on Portobello mushrooms, but has a lower impact on Bacillus cereus. Vacuum-packed dried mushrooms further reduce Listeria, while Salmonella and Bacillus cereus remain stable. Rehydrating dried mushrooms improperly can lead to regrowth of all three organisms.
Article
Food Science & Technology
Miguel Aguilera-Toro, Martin Laage Kragh, Amalie Vestergard Thomasen, Vittoria Piccini, Valentin Rauh, Yinghua Xiao, Lars Wiking, Nina A. Poulsen, Lisbeth Truelstrup Hansen, Lotte B. Larsen
Summary: This study assessed the proteolytic activity of 56 Pseudomonas strains and found that the sequence similarity of the aprX-lipA operon significantly influenced the proteolytic activity. The average proteolytic activity of the strains was higher in the A1 group compared to the A2, B, and N groups. The proteolytic activity of the strains was not affected by the lab-scale UHT treatment, indicating high thermal stability. Conserved motifs in the AprX sequence could serve as potential genetic biomarkers for determining strain spoilage potential.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Food Science & Technology
Jonas Steenholdt Sorensen, Sofie Rugh van Reeuwijk, Roy S. Bartle, Lisbeth Truelstrup Hansen
Summary: The processing of seaweed often involves low-temperature drying to stabilize the product by inactivating and inhibiting the growth of microorganisms. However, Salmonella can survive dry conditions and persist in low-moisture food, which has been linked to foodborne outbreaks from seaweed. This study presents drying and desorption models for Alaria esculenta and a model for the inactivation kinetics of S. Typhimurium during low heat convection drying.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Environmental
Claus Jorgensen, Nina D. Sto. Domingo, Berislav Tomicic, Morten E. Jorgensen, Lisbeth Truelstrup Hansen, Heidi Huus Petersen, Jes Clauson-Kaas
Summary: Urban cloudburst management may involve temporarily storing flood water in green recreational areas, but this can expose visitors to sewage and increase the risk of diarrhoeal disease. This study presents a unique approach to estimate the risk of diarrhoeal disease after urban flooding by modeling the transport of sewage into flooded parks and estimating the transfer of pathogens to grass and from grass to hand. The estimated probability of illness varied from 0.03% to 17%, and if deemed unacceptable, changes to cloudburst plans or interventions such as public risk awareness or restricted access to flooded areas should be considered.
WATER SCIENCE AND TECHNOLOGY
(2023)
Article
Ecology
Katharina J. Kreissig, Jonas Steenholdt Sorensen, Pernille Erland Jensen, Lisbeth Truelstrup Hansen
Summary: Seaweed from Greenland has potential as a food source, but it may become contaminated by human pathogens due to the discharge of human sewage into the sea. This study investigated the effect of wastewater discharge on the bacterial communities of wild populations of Fucus sp. in two different communities in Greenland. The results showed significant differences in the microbiota of seaweed harvested in areas with and without wastewater discharge, indicating the presence of human pathogens in the contaminated seaweed.
REGIONAL STUDIES IN MARINE SCIENCE
(2023)
Article
Infectious Diseases
Meiyao Che, Tina Birk, Lisbeth Truelstrup Hansen
Summary: This study reveals the presence and transmission of extended-spectrum cephalosporin-resistant Escherichia coli in the Danish broiler production system, highlighting the potential for these resistant bacteria to enter the food supply chain.
Article
Public, Environmental & Occupational Health
Judith Y. A. Marechal, Kare Hendriksen, Lisbeth Truelstrup Hansen, Casper Gundelund, Pernille Erland Jensen
Summary: This study examined the water supply situation in rural Greenland and found that while the supply of drinking water is sufficient and affordable, access is severely restricted due to lack of piping. Residents of un-piped households have insufficient water consumption to sustain public health and this could lead to water quality degradation.
INTERNATIONAL JOURNAL OF CIRCUMPOLAR HEALTH
(2022)
Meeting Abstract
Chemistry, Applied
Hanne Jensen, Charlotte Jacobsen, Niels Boknaes, Lisbeth Hansen, Ole Mejlholm
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2021)
Article
Multidisciplinary Sciences
Yannan Huang, Lindsay Johnston, Ana Parra, Crystal Sweeney, Emalie Hayes, Lisbeth Truelstrup Hansen, Graham Gagnon, Amina Stoddart, Rob Jamieson
Summary: Wastewater-based surveillance methods have been used in various countries to monitor the presence of SARS-CoV-2 in communities. A study in Halifax, Nova Scotia, found that the virus could be detected in wastewater samples, with different RT-qPCR assays showing varying sensitivity in detecting the virus. The CDC N1 and E RT-qPCR assays demonstrated higher sensitivity compared to the CDC N2 and N3 assays.
