Article
Chemistry, Analytical
Viktorija Teiserskyte, Jaunius Urbonavicius, Dalius Ratautas
Summary: This study reported the construction of a direct electron transfer biosensor based on NAD-dependent formaldehyde dehydrogenase, achieving a fast, highly sensitive detection of formaldehyde with high stability and selectivity.
Article
Chemistry, Analytical
Miriam Chavez, Angela Fernandez-Merino, Rafael del Cano, Guadalupe Sanchez-Obrero, Rafael Madueno, Manuel Blazquez, Teresa Pineda
Summary: Electrochemical biosensors composed of hemoproteins and anisotropic gold nanoparticles were proposed, and their electrochemical properties were enhanced and immobilized. The biosensors exhibited direct redox responses, and the gold nanotriangles acted as mediators for electron transfer. The stability of the hemoproteins was demonstrated in the reduction of hydrogen peroxide, oxygen, and nitrite. These biosensors have attractive properties and potential for advancement in sensing devices.
Article
Biochemistry & Molecular Biology
Sayaka Nishida, Hinata Sumi, Haruna Noji, Akira Itoh, Kunishige Kataoka, Satoshi Yamashita, Kenji Kano, Keisei Sowa, Yuki Kitazumi, Osamu Shirai
Summary: In this study, the effects of N-glycan composition on BOD's enzymatic orientation and DET-type reactions were evaluated using maleimide-functionalized polyethylene glycol (MAL-PEG) as an N-glycan mimic.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Yu Cai, Tingting Tu, Tianyu Li, Shanshan Zhang, Bin Zhang, Lu Fang, Xuesong Ye, Bo Liang
Summary: The study on the application of nanomaterials in glucose biosensors demonstrates excellent electrochemical performance, taking a step towards the development of the third-generation glucose biosensor.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Alexander Trifonov, Andreas Stemmer, Ran Tel-Vered
Summary: A new practical approach for extending the operation lifetimes of bioelectrocatalytic assemblies based on bilirubin oxidase (BOD) is presented in this study by adsorption of carbon-coated magnetic nanoparticles (ccMNPs) onto the BOD/carbon nanotubes-deposited surface. The modified assemblies show an 80% increase in active enzymatic content and electrocatalytic performance over a course of one month. The method of using external magnetic force to remove protective particles before measurement is simple, reproducible, and easy to implement, suggesting a promising methodology for enhancing the lifetimes of bioelectronic applications.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yohei Suzuki, Akira Itoh, Kunishige Kataoka, Satoshi Yamashita, Kenji Kano, Keisei Sowa, Yuki Kitazumi, Osamu Shirai
Summary: This study investigated the effect of N-glycans on DET-type bioelectrocatalysis and found that the size and composition of N-glycans influenced the orientation of the enzyme on the electrode. Enzymes with larger N-glycans achieved the most favorable orientation, and the effect of N-glycans on orientation was cooperative with electrostatic interaction.
BIOELECTROCHEMISTRY
(2022)
Article
Engineering, Chemical
T. D. Le, D. Lasseux, L. Zhang, C. Carucci, S. Gounel, S. Bichon, F. Lorenzutti, A. Kuhn, T. Safarik, N. Mano
Summary: This work focuses on a multi-scale modeling of coupled diffusion and reaction in a porous micro electrode operating in the Direct Electron Transfer mode. By developing and validating the model, it provides an operational simple way of understanding and further optimizing the functioning of porous electrodes in DET mode.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Chemistry, Analytical
Shalev Gihaz, Nidaa Shrara Herzallh, Yifat Cohen, Oren Bachar, Ayelet Fishman, Omer Yehezkeli
Summary: This study presents the first crystal structure of bilirubin oxidase (BOD) from Bacillus pumilus (BpBOD) at 3.5 angstrom resolution. BpBOD shares high homology with fungal enzymes but possesses a unique surface-exposed disulfide bond between Cys229 and Cys322 residues. The developed methodologies to orient the T1 site towards the electrode by coupling the reduced disulfide bond with a maleimide moiety on the electrodes resulted in improved direct electron transfer rates.
Article
Biophysics
Hai-Hua Wang, Meng-Jie Li, Yu-Peng Tu, Hai-Jun Wang, Ya-Qin Chai, Zhao-Hui Li, Ruo Yuan
Summary: This study introduced a novel photoelectrochemical biosensor utilizing a fullerene derivative for amplification-free ultrasensitive detection of single-stranded DNA, demonstrating a wide linear range and low detection limit.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Physical
Shaoguang Zhang, Serge Ruccolo, Anna Fryszkowska, Artis Klapars, Nicholas Marshall, Neil A. Strotman
Summary: An electrochemical activation method for galactose oxidase (GOase) was developed to replace the expensive horseradish peroxidase activator in large-scale pharmaceutical processes. The study showed that the electron-transfer rates between GOase and various mediators depend on both the redox potential of the mediator and the pH. The electrochemical GOase activation method was successfully applied to the aerobic oxidation of benzyl alcohol derivatives and other compounds, including a key step in the biocatalytic cascade for the HIV therapeutic islatravir.
Article
Biophysics
Kentaro Hiraka, Wakako Tsugawa, Ryutaro Asano, Murat A. Yokus, Kazunori Ikebukuro, Michael A. Daniele, Koji Sode
Summary: This study successfully developed the first dual sensing system for lactate and glucose based on DET-type enzymes, enhancing detection range and reducing substrate inhibition through fusion enzymes and mutation. The developed sensor exhibited excellent performance in artificial sweat, enabling simultaneous detection of lactate and glucose.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Rachna Rawal, Poonam R. Kharangarh, Sudhir Dawra, Preetam Bhardwaj
Summary: A high-power ultrasonic method was used to prepare bilirubin oxidase nanoparticles immobilized on polyethylene film, showing optimal activity at pH 8 with an incubation time of 11 seconds and temperature of 35 degrees Celsius. The bound nanoparticles exhibited good correlation for bilirubin values and satisfactory reliability, with high sensitivity and repeatability.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Joonyoung Lee, Ki-Yoon Kim, Yongchai Kwon, Dahl-Young Khang
Summary: This study proposes stretchable enzymatic biofuel cells (EBFCs) with high performance for power supply. The buckled gold electrodes increase the electrode surface area and catalyst loading, resulting in improved reaction rates. The evaluated EBFCs achieve a maximum power density of 7.1±0.64μW cm-2.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Physical
I-Hsuan Li, Tien-Hao Hsieh, Zhi-Wen Chen, Hsiang-Chun Tsao, Yu-Hsuan Huang, Ching-Ping Liu
Summary: Composites or hybrids, such as SiO2-AuNPs, have been investigated for their enhanced catalytic performance and new catalytic activities. This study demonstrates that SiO2-AuNPs exhibit both peroxidase-like and oxidase-like activities, with the contribution of SiO2 mainly involved in the peroxidase-like reaction. Furthermore, the oxidase-like activity of SiO2-AuNPs can be enhanced by visible light irradiation, and electron transfer between SiO2 and AuNPs is also observed.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Chemistry, Physical
Umberto Contaldo, Solene Gentil, Elise Courvoisier-Dezord, Pierre Rousselot-Pailley, Fabrice Thomas, Thierry Tron, Alan Le Goff
Summary: We report the unprecedented use of laccase to functionalize phenol-modified carbon nanotubes (CNTs). Enzymatically-generated metallopolyphenols or the laccase itself can be mildly and efficiently immobilized using the ability of laccase to generate phenoxyl radicals. Electrochemistry, XPS and EPR spectroscopy were used to assess the enzyme-catalyzed CNT functionalization. The efficient immobilization of laccase is confirmed by the high direct bioelectrocatalytic reduction of oxygen with a maximum current density of 1.95 mA cm(-2).
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Review
Electrochemistry
Cristina Gutierrez-Sanchez, Encarnacion Lorenzo
Summary: Recent advances in the development of nanoporous surfaces and their modification with biomolecules have enabled important bioelectrochemical applications. New nanoporous surfaces with controlled architecture improve the properties of traditional surfaces, resulting in larger specific surface areas, higher conductivity, and better electrochemical activity. The challenge lies in achieving suitable pore size, arrangement, and distribution to facilitate substrate transport and enzyme orientation. The objective is to obtain an ideal nanoporous surface that offers a large surface area, efficient substrate transport, and effective immobilization of redox enzymes.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Materials Science, Multidisciplinary
Inigo Torres, Ana Maria Villa-Manso, Monica Revenga-Parra, Cristina Gutierrez-Sanchez, Diego A. Aldave, Elena Salagre, Enrique Garcia Michel, Maria Varela, Julio Gomez-Herrero, Encarnacion Lorenzo, Felix Pariente, Felix Zamora
Summary: This study reports the successful preparation of high-quality few-layer bismuthene using a two-step procedure under mild conditions. The resulting bismuthene hexagons show excellent electrical properties, making them suitable for electronic devices.
APPLIED MATERIALS TODAY
(2022)
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Cristina Gutierrez-Sanchez, Ana M. Villa-Manso, Monica Revenga-Parra, Felix Pariente, Encarnacion Lorenzo
Summary: Given the importance of fast, efficient, and cost-effective detection of SARS-CoV-2 in wastewater to control the pandemic, a new method based on a carbon nanodots-amplified electrochemiluminescence immunosensor has been proposed. The immunosensor showed high specificity and stability, with a detection limit of 1.2 pg/mL, and successfully detected SARS-CoV-2 in river and urban wastewater. This sensing platform provides a feasible and reliable tool for early virus detection and protection of the population.
Article
Chemistry, Physical
Jose M. Abad, Marcos Pita, Antonio L. De Lacey
Summary: Monolayer-Protected Gold Clusters (AuMPCs) have attracted great interest as nanoscale capacitors in aqueous media for various nanobiotechnological applications. However, achieving subattofarad double-layer capacitance and single-electron charging has been challenging. In this study, we successfully synthesized Thioctic Acid Monolayer-Protected Au Clusters (TA-AuMPCs) that exhibited electrochemical single electron quantized capacitance charging in both organic and aqueous solutions. The functional carboxylic groups present in TA-AuMPCs provide a simple strategy for interfacing nanoparticles with biomolecules for biological electron transfer reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Analytical
Emiliano Martinez-Perinan, Aitor Dominguez-Saldana, Ana M. Villa-Manso, Cristina Gutierrez-Sanchez, Monica Revenga-Parra, Eva Mateo-Marti, Felix Pariente, Encarnacion Lorenzo
Summary: Carbon nanodots modified with azure A were synthesized and applied as a redox mediator for bioelectrocatalytic reactions. The modified electrodes demonstrated excellent electrocatalytic activity and analytical performance for the detection of NADH and glutamate. The developed biosensor based on immobilized enzyme showed good analytical parameters and successfully analyzed food and biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Melisa del Barrio, Emiliano Martinez-Perinan, Cristina Gutierrez-Sanchez, Eva Mateo-Marti, Marcos Pita, Antonio L. De Lacey, Felix Pariente, Encarnacion Lorenzo
Summary: The determination of cholesterol is important for heart disease detection and as a fuel for enzymatic fuel cells. A cholesterol biosensor and peroxide-free biofuel cell was developed using electrocatalytic oxidation of NADH generated during cholesterol dehydrogenase (ChDH) reaction. This alternative method avoids the use of H2O2 biosensing strategies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Gabriel Luna-Lopez, Melisa del Barrio, Jennifer Fize, Vincent Artero, Ana Margarida Coito, Ines A. C. Pereira, Jose Carlos Conesa, Ana Iglesias-Juez, Antonio L. De Lacey, Marcos Pita
Summary: Clean energy vectors are required to achieve a fossil fuel-free society, reducing both greenhouse effect and pollution. Electrochemical water splitting provides a clean method to obtain green hydrogen, but efficient electrocatalysts are needed to avoid high overpotentials. The combination of inorganic semiconductors with biocatalysts offers an alternative approach for photoelectrochemical H2 production.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Physical
Mihai-Cristian Fera, Rita R. Manuel, Ines A. C. Pereira, Jose M. Abad, Antonio L. De Lacey, Marcos Pita
Summary: The electrochemical reduction of CO2 through redox enzymes is a promising method to reduce global CO2 concentrations. In this study, the enzyme Desulfovibrio vulgaris Hildenborough FdhAB formate dehydrogenase was effectively immobilized on MWCNTs-modified low density graphite electrodes using electrostatic interactions and protected with polyethyleneimine (PEI) polymer. The synergistic effect of MWCNTs and PEI resulted in high catalytic current densities and long-term operational stability.
Article
Chemistry, Analytical
Ana M. Villa-Manso, Tamara Guerrero-Esteban, Felix Pariente, Celia Toyos-Rodriguez, Alfredo de la Escosura-Muniz, Monica Revenga-Parra, Cristina Gutierrez-Sanchez, Encarnacion Lorenzeo
Summary: A novel immunosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) has been developed for the sensitive determination of N protein of the SARS-CoV-2 coronavirus. The immunosensor utilizes bifunctional core@shell nanoparticles as ECL acceptor and [Ru(bpy)(3)](2+) as ECL donor. It demonstrates a wide linear response to the concentration of N protein with a detection limit of 1.27 pg/mL, high stability, and specificity. The immunosensor has been successfully applied for quantification of N protein in saliva samples and showed consistent results with a commercial colorimetric ELISA kit.
Article
Nanoscience & Nanotechnology
Keyvan Jodeiri, Aleksandra Foerster, Gustavo F. Trindade, Jisun Im, Diego Carballares, Roberto Fernandez-Lafuente, Marcos Pita, Antonio L. De Lacey, Christopher Parmenter, Christopher Tuck
Summary: The drive for miniaturization in enzyme-based bioelectronics has led to the development of 3D microstructured electrodes. Additive manufacturing combined with electroless metal plating allows the production of these electrodes with high surface area. The challenge lies in ensuring strong adhesion between the metal layer and polymer structure, which has been achieved in this study through the introduction of an interfacial adhesion layer.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Laura Fernandez-Lopez, Isabel Cea-Rama, Julia Alvarez-Malmagro, Anna K. Ressmann, Jose L. Gonzalez-Alfonso, Cristina Coscolin, Patrick Shahgaldian, Francisco J. Plou, Jan Modregger, Marcos Pita, Julia Sanz-Aparicio, Manuel Ferrer
Summary: Metal complexes introduced into protein scaffolds can generate versatile biomimetic catalysts with various catalytic properties. In this study, we synthesized and covalently bound a bipyridinyl derivative to the active site of an esterase, resulting in a biomimetic catalyst that exhibits catecholase activity and enantioselective catalytic oxidation of (+)-catechin.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Tamara Guerrero-Esteban, Borja L. Sanchez, Lucia Exposito, David Rodriguez-San-Miguel, Felix Zamora, Felix Pariente, Cristina Gutierrez-Sanchez, Encarnacion Lorenzo
Summary: This work presents a novel approach for detecting biogenic amine tyramine using a sensitive and disposable electrochemiluminescent sensor. The sensor is fabricated by modifying a screen-printed carbon electrode surface with two nanomaterials, alpha-Ge nanolayers and AuNP, which synergistically enhance the electrochemiluminescence response. The sensor was characterized using various techniques such as SEM-EDX, EIS, Raman, and AFM. The principle of the biosensor relays on the fact that tyramine molecule acts as an analyte and co-reactant, which interacts with the luminophore [Ru(bpy)(3)](2+) on the sensor surface. The proposed sensor shows a linear response to tyramine concentration, with a detection limit of 2.28 mu M. The sensor successfully detected tyramine in avocado samples, demonstrating its potential for practical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo
Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta
Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin
Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere
Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs
Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao
Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran
Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiaopeng Hou, Qiongyi Cheng, Hui Wang
Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene
Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.
BIOELECTROCHEMISTRY
(2024)
