Article
Chemistry, Analytical
Miriam Chavez, Angela Fernandez-Merino, Rafael del Cano, Guadalupe Sanchez-Obrero, Rafael Madueno, Manuel Blazquez, Teresa Pineda
Summary: Electrochemical biosensors composed of hemoproteins and anisotropic gold nanoparticles were proposed, and their electrochemical properties were enhanced and immobilized. The biosensors exhibited direct redox responses, and the gold nanotriangles acted as mediators for electron transfer. The stability of the hemoproteins was demonstrated in the reduction of hydrogen peroxide, oxygen, and nitrite. These biosensors have attractive properties and potential for advancement in sensing devices.
Article
Biophysics
Marie-Christin Viehauser, Erik Breslmayr, Stefan Scheiblbrandner, Franziska Schachinger, Su Ma, Roland Ludwig
Summary: The development of third generation biosensors relies on enzymes capable of direct electron transfer (DET), achieved by fusing a cytochrome domain to an enzyme. In this study, the chimeric enzyme NcCYT-GcGDH was successfully generated in large quantities, showing high catalytic activity and the ability to perform interdomain electron transfer and direct electron transfer via the fused cytochrome domain. The high current obtained on a simple, unmodified electrode at a relatively low redox potential provides a promising starting point for further optimization.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Hai-Hua Wang, Meng-Jie Li, Yu-Peng Tu, Hai-Jun Wang, Ya-Qin Chai, Zhao-Hui Li, Ruo Yuan
Summary: This study introduced a novel photoelectrochemical biosensor utilizing a fullerene derivative for amplification-free ultrasensitive detection of single-stranded DNA, demonstrating a wide linear range and low detection limit.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Electrochemistry
Kavita Jayakumar, Thomas M. B. Reichhart, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Donal Leech
Summary: Cellobiose dehydrogenase (CDH) is a biocatalyst capable of oxidizing cellobiose and related carbohydrates to generate electrical current. A novel engineered CDH has shown improved performance as a bioelectrocatalyst in glucose biosensors.
Article
Electrochemistry
Xiaomei Yan, Su Ma, Jing Tang, David Tanner, Jens Ulstrup, Xinxin Xiao, Jingdong Zhang
Summary: This study systematically investigates the direct electrochemical electron transfer of the membrane-bound redox enzyme fructose dehydrogenase on Au and nanoporous gold electrodes. It reveals the optimal activity performance under different thiol self-assembled monolayers and offers mechanistic insights into the process.
ELECTROCHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Roy Cohen, Nidaa S. Herzallh, Matan M. Meirovich, Oren Bachar, Liora Frech, Yifat Cohen, Omer Yehezkeli
Summary: Lactate sensing is important for various applications such as metabolic disease diagnostics and biofuel cell devices. In this study, we developed a continuous amperometric lactate biosensor using overexpressed FMN-lactate dehydrogenase from Saccharomyces cerevisiae. The sensor exhibited high signal-to-noise ratio, low interference effect, and wide range of linear responses.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Xiaorong Liu, Chaonan He, Qi Huang, Mengmeng Yu, Zhuang Qiu, Haoxin Cheng, Yifei Yang, Xian Hao, Xiaolei Wang
Summary: In this study, a simple visualized solid-phase biosensor based on luminescence resonance energy transfer (LRET) was constructed for quantitative detection of virus-specific nucleic acid sequences. The linear relationship between the luminescence recovery of upconversion nanoparticles (UCNPs) and the target concentrations within the range of 5-100 nM was found, and the limit of detection (LOD) was determined to be 0.326 nM.
Article
Chemistry, Analytical
Kikuo Komori, Shinnosuke Takumi, Kiichi Kato, Kazuya Matsumoto, Kohei Shiraishi, Hiroshi Kimura, Kazutake Takada
Summary: Due to histamine's role in allergic reactions, there is a need for efficient detection methods and real-time monitoring systems for food analysis and drug discovery to suppress allergic reactions. In this study, the researchers investigated the direct electrochemistry of histamine dehydrogenase (HmDH) on the surface of graphite nanofibers (GNFs), which provide active reaction sites. They found that the air plasma-treated GNFs improved the direct electron transfer from reduced HmDH to the GNFs, possibly due to the presence of oxygen-containing functional groups generated by the treatment. Additionally, the air plasma-treated GNFs enhanced the rate of complex-formation reaction between HmDH and histamine.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rajendra Kumar Reddy Gajjala, Pravin Savata Gade, Praveena Bhatt, Neelam Vishwakarma, Suman Singh
Summary: Recent studies have focused on bi- and tri-metallic nanocomposites to enhance catalytic surface and detection sensitivity. A biosensor based on formaldehyde dehydrogenase decorated Cys-AuPd-ErGO nanocomposite was successfully developed for NADH and HCHO detection, showing direct electron transfer and high sensitivity. The sensor demonstrated excellent performance in detecting HCHO in adulterated samples of fish and milk, with recoveries of 88-104%, and was validated against HPLC method with a correlation coefficient of 0.99.
Article
Chemistry, Multidisciplinary
Zedong Li, Shuang Wu, Jingcheng Ji, Yuemeng Bai, Pengpeng Jia, Yan Gong, Shangsheng Feng, Fei Li
Summary: Lateral flow assays (LFAs), a popular point-of-care testing platform, have limited detection sensitivity. This study developed a ball pen writing-without-ink method to amplify the detection signal, resulting in improved sensitivity.
Article
Engineering, Environmental
Bin Zhu, Qi-Wei Li, Ye-Cheng Li, Ya-Qiang Xia, Jing -Lin Liu, A-Min Zhou, Xiao-Min Zhang
Summary: Plasma-catalysis technique is a promising approach to control air pollution caused by volatile organic compounds (VOCs). Perovskite-type oxides have excited much interest in constructing plasma-catalytic system for the oxidation removal of VOCs owing to their flexible electronic structure, alterable geometry, unique catalytic properties and high stability.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Food Science & Technology
Hasan Nasiri, Hamed Baghban, Reza Teimuri-Mofrad, Ahad Mokhtarzadeh
Summary: Graphitic carbon nitride supported on magnetic chitosan was synthesized and used as a support for cellobiose dehydrogenase immobilization and as a modifier for glassy carbon electrode in the fabrication of an electrochemical lactose sensor. The composite exhibited excellent electrochemical performance and was successfully applied in lactose quantification in dairy products.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Chemistry, Analytical
Yu Cai, Tingting Tu, Tianyu Li, Shanshan Zhang, Bin Zhang, Lu Fang, Xuesong Ye, Bo Liang
Summary: The study on the application of nanomaterials in glucose biosensors demonstrates excellent electrochemical performance, taking a step towards the development of the third-generation glucose biosensor.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Charlotte Uldahl Jansen, Xiaomei Yan, Jens Ulstrup, Xinxin Xiao, Katrine Qvortrup
Summary: This study investigates the use of small molecule bridges and various interactions to control the alignment of enzymes on electrodes. Testing anthraquinone derivatives, it is found that those functionalized with anionic sulfonate groups exhibit the best ability for DET.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Editorial Material
Multidisciplinary Sciences
Carla Umansky, Agustin E. Morellato, Lucas B. Pontel
Summary: The study introduced a genetically encoded sensor for formaldehyde detection in cells and tissues, shedding light on an elusive cellular metabolite and potentially revolutionizing formaldehyde research.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Jingjing Zhou, Ludovic Pecqueur, Agota Aucynaite, Jonathan Fuchs, Rasa Rutkiene, Justas Vaitekunas, Rolandas Meskys, Matthias Boll, Marc Fontecave, Jaunius Urbonavicius, Beatrice Golinelli-Pimpaneau
Summary: This study reports the crystal structure of a [Fe-S]-containing protein called TudS and its mechanism in the desulfuration of thiouracil. The [4Fe-5S] state of the cluster was identified as a catalytic intermediate, with a water molecule and catalytically important residues Ser101 and Glu45 playing key roles in the reaction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Ronaldas Jakubovskis, Augusta Jankute, Simona Guobuzaite, Renata Boris, Jaunius Urbonavicius
Summary: The study explores the use of coated expanded clay as a bacterial carrier in biological concrete. Results show that more viable bacteria improve crack closure ratio and enhance crack healing efficiency in biological concrete. The additional coating of EC particles improves long-term bacterial viability.
Article
Chemistry, Physical
Eimantas Ramonas, Atefeh Shafaat, Marius Dagys, Tautgirdas Ruzgas, Dalius Ratautas
Summary: This study investigated the catalytic properties of citrate-capped gold nanoparticles and gold nanoparticle-protein conjugates, showing that gold nanoparticles form stable conjugates with proteins but do not increase enzyme activity. Under certain conditions, gold nanoparticles can mimic enzymatic reactions, emphasizing the need for specific measurements to differentiate between enzyme and gold nanoparticle-catalyzed reactions.
JOURNAL OF CATALYSIS
(2021)
Article
Construction & Building Technology
Ronaldas Jakubovskis, Augusta Ivaske, Jurgita Malaiskiene, Jaunius Urbonavicius
Summary: This paper analyzes the impact of cement type on bacterial viability and reveals the inhibitory effects of specific metal oxides on bacteria. It suggests analyzing the chemical composition of the chosen cement type and studying the activity of compounds that inhibit bacteria before producing biological concrete.
CEMENT & CONCRETE COMPOSITES
(2022)
Article
Chemistry, Multidisciplinary
Atefeh Shafaat, Rokas Zalneravicius, Dalius Ratautas, Marius Dagys, Rolandas Meskys, Rasa Rutkiene, Juan Francisco Gonzalez-Martinez, Jessica Neilands, Sebastian Bjorklund, Javier Sotres, Tautgirdas Ruzgas
Summary: This study introduces a simple and wireless biosensor design for glucose detection by incorporating an AgCl layer into the antenna. The wireless biosensor showed good agreement with a commercially available glucometer in measuring glucose in whole blood.
Article
Ecology
Saloua Biyada, Mohammed Merzouki, Taisija Demcenko, Dovile Vasiliauskiene, Egle Marciulaitiene, Saulius Vasarevicius, Jaunius Urbonavicius
Summary: In this study, the effect of initial feedstock concentration on the microbial community structure and dynamics during textile waste composting processes was investigated. It was found that increasing the concentration of textile waste led to changes in microbial communities and enzyme activities. Certain bacterial and fungal genera and species were most abundant in mixtures containing 40% or 60% textile waste, while some species were absent in mixtures with 80% textile waste.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2022)
Article
Biophysics
Skomantas Serapinas, Justina Gineityte, Marius Butkevicius, Rapolas Danilevicius, Marius Dagys, Dalius Ratautas
Summary: This paper presents a new bioelectronic device for the detection of nuclease activity in various fluids. The developed sensor enables rapid and sensitive determination of DNase activity, with high accuracy and reproducibility demonstrated in real human saliva samples. The technology has the potential to improve nuclease quality control processes in the pharmaceutical/biotechnology industry and provide new insights into the importance of nucleases for medical applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Eimantas Ramonas, Marius Butkevicius, Sergey Shleev, Marius Dagys, Dalius Ratautas
Summary: The study introduces a novel concept of an enzyme-driven pH electrode system composed of two electrodes, which requires no additional counter or reference electrodes and provides high-precision real-time pH monitoring. Additionally, an in-depth mechanistic study was presented to explain the relationship between electrode potential and pH dependence, offering new insights into the operational principles of bilirubin oxidase at different pH levels.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Justas Miskinis, Eimantas Ramonas, Vidute Gureviciene, Julija Razumiene, Marius Gagys, Dalius Ratautas
Summary: In this paper, a biosensor based on a gold nanoparticle-modified platinum electrode was developed for the detection and quantification of total L-amino acids. The biosensor showed high sensitivity and a low limit of detection for hydrogen peroxide and L-amino acids, and could be applied for the measurement of relative loss of amino acids in patients undergoing renal replacement therapy. The capacitance-based measurement method used in conjunction with the biosensor electrode played a crucial role in achieving accurate measurements of low amino acid concentrations.
Article
Microbiology
Dovile Vasiliauskiene, Renata Boris, Giedrius Balciunas, Agne Kairyte, Jaunius Urbonavicius
Summary: This study investigated the microorganisms growing on biocomposite boards with different flame retardants and their effects on the material properties. The results showed that the flame retardants led to deterioration in density and compressive strength of the boards, and expandable graphite also acted as an antimicrobial agent.
Article
Microbiology
Dovile Vasiliauskiene, Giedrius Balciunas, Renata Boris, Agne Kairyte, Jaunius Urbonavicius
Summary: The study evaluated the performance characteristics of hemp shives impregnated with linseed oil and tung tree oil-based biocomposites containing flame retardants before and after treatment with a mixture of bacterium P. putida and fungus Rh. oryzae. The properties tested include enzymatic activities, water absorption, thickness swelling, compressive strength, and thermal conductivity. It was found that linseed oil provides better protection against microbial action compared to tung tree oil.
Review
Engineering, Chemical
Saloua Biyada, Mohammed Merzouki, Jaunius Urbonavicius
Summary: Energy conservation is a strategic goal worldwide, and energy consumption in buildings is a major source of energy consumption in many countries. The commonly used insulation materials have harmful impacts on the environment, so it is important to develop insulation materials that have less environmental impact and are cost-effective. Agricultural and industrial wastes, as well as natural fibers, are increasingly being used as green insulation materials due to their eco-friendliness and non-critical end-of-life cycle.
Article
Microbiology
Augusta Ivaske, Viktor Gribniak, Ronaldas Jakubovskis, Jaunius Urbonavicius
Summary: This study investigates the viability of bacteria encapsulated in cementitious materials for crack healing in concrete structures. The results show that B. pseudofirmus demonstrated the best mineralization activity and viability.
Article
Chemistry, Analytical
Julija Razumiene, Damiana Leo, Vidute Gureviciene, Dalius Ratautas, Justina Gaidukevic, Ieva Sakinyte-Urbikiene
Summary: Investigations of L-glutamate release can help identify new pathophysiological pathways related to L-glutamate and neurological diseases. A nitrogen-modified graphene oxide biosensor was developed through a one-pot hydrothermal reduction method and used to detect L-glutamate. The biosensor demonstrated stability, ease of preparation, and successful detection of L-glutamate in real samples. Furthermore, it was applied to investigate L-glutamate release in a specific rat strain.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
