Review
Biochemistry & Molecular Biology
Chu-Qiao Liang, Yan-Mei Li
Summary: The review highlights recent progress in peptide-based inhibitors, including amyloid sequence-derived inhibitors, designed peptides, and peptide mimics, towards pathological amyloid proteins and deposition. These peptides exhibit advanced inhibitory activities against fibrous aggregation as well as enhanced druggability, based on increased understanding of peptide design and precise amyloid structures.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kobe Janssen, Filip Claes, Dido Van de Velde, Vanessa L. Wehbi, Bert Houben, Yulia Lampi, Mieke Nys, Laleh Khodaparast, Ladan Khodaparast, Nikolaos Louros, Rob van der Kant, Joffre Verniers, Teresa Garcia, Meine Ramakers, Katerina Konstantoulea, Katerina Maragkou, Ramon Duran-Romana, Monica Musteanu, Mariano Barbacid, Bernard Scorneaux, Els Beirnaert, Joost Schymkowitz, Frederic Rousseau
Summary: Mutant KRAS is a challenging target for classical small molecule drugs, but this study shows that its misfolding can be induced by synthetic peptides derived from specific regions of the oncoprotein. These peptides were able to inhibit the function of mutant KRAS and showed antiproliferative activity against cancer cells. The findings provide proof-of-concept for exploiting the intrinsic misfolding propensity of KRAS as a therapeutic strategy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Wenbo Zhang, Mingwei Liu, Lanlan Yu, Shanshan Mo, Zhun Deng, Shuli Liu, Yanlian Yang, Chen Wang, Chenxuan Wang
Summary: This study demonstrates the contribution of various noncovalent interactions to supramolecular assembly by investigating the self-assembly of peptides.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Pangmiaomiao Zhang, Chunyan Tan
Summary: In this study, a fluorescent sensor array was developed to detect A beta aggregates and monitor their aggregation kinetics, providing a convenient approach for studying neurodegenerative diseases.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Liisa Lutter, Liam D. Aubrey, Wei-Feng Xue
Summary: Predicting the highly ordered three-dimensional structures of amyloid protein fibrils from their monomeric self-assembly precursors remains a challenging aspect of the classical protein folding problem, due to the polymorphic nature of amyloid assembly. Understanding the diversity and individuality of amyloid structures, as well as the link to biology and disease phenotypes, is essential. Current research focuses on resolving the structural basis and biological consequences of polymorphic amyloid assemblies using various techniques such as cryo-EM, ssNMR, and AFM.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Nadejda Koloteva-Levine, Liam D. Aubrey, Ricardo Marchante, Tracey J. Purton, Jennifer R. Hiscock, Mick F. Tuite, Wei-Feng Xue
Summary: Amyloid seeds are nanometer-sized protein particles that can accelerate amyloid formation through a surface catalysis mechanism without propagating the specific amyloid conformation associated with the seeds. This allows for cross-seeded assembly reactions involving nonhomologous proteins and rationalizes the molecular mechanism of the cross-seeding phenomenon as a manifestation of aberrant surface activities by amyloid seeds.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
Jad Kaj, Dayan Hussein, Pierre Karam
Summary: In this study, a fluorescent material with temperature memory properties was developed. The material can detect exposure to a wide range of temperatures and has a strong temperature memory effect.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Biochemical Research Methods
Anna Yudenko, Anastasia Smolentseva, Ivan Maslov, Oleg Semenov, Ivan M. Goncharov, Vera V. Nazarenko, Nina L. Maliar, Valentin Borshchevskiy, Valentin Gordeliy, Alina Remeeva, Ivan Gushchin
Summary: Protein-fragment complementation assays are commonly used to study protein-protein interactions. The development of CagFbFP, a small thermostable FbFP based on a LOV domain-containing protein, provides a split fluorescent reporter that can be used to investigate protein-protein interactions in anaerobic conditions without the need for exogenous fluorophores. This advancement in fluorescent protein technology may lead to further development of LOV and PAS domain-based fluorescent reporters and optogenetic tools.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thorbjorn Sonderby, Helena O. Rasmussen, Signe A. Frank, Jan Skov Pedersen, Daniel E. Otzen
Summary: Functional bacterial amyloids (FuBA) are rapidly and efficiently aggregating intrinsically disordered proteins (IDPs) that form extremely stable fibrils. The conversion from IDPs to amyloids is evolutionarily optimized and likely links folding to association. Many FuBA contain several imperfect repeat sequences which contribute to the stability of mature FuBA fibrils.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Engineering, Chemical
Yanxian Zhang, Yijing Tang, Dong Zhang, Yonglan Liu, Jian He, Yung Chang, Jie Zheng
Summary: The research focuses on the mechanism of amyloid cross-seeding between Aβ and hIAPP and its connection between AD and T2D, explores the role of structural compatibility and sequence similarity of amyloid proteins in cross-seeding, and proposes current challenges and future research directions in this less-studied field.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Cell Biology
Henry Patrick Oamen, Nathaly Romero Romero, Philip Knuckles, Juha Saarikangas, Marta Radman-Livaja, Yuhong Dong, Fabrice Caudron
Summary: This study found that the natural lipid tripentadecanoin can delay the formation of protein aggregates and extend cellular lifespan, offering a potential treatment for neurodegenerative diseases. Tripentadecanoin can also protect neurons from amyloid toxicity and promote the recovery of retinal cells.
Article
Hematology
Sally Bevan, Colin Longstaff
Summary: The study found that the instability of frozen-dried D-dimer plasma may result in 10%-18% loss of reactivity per year when stored at -20 degrees Celcius. Freeze-dried fibrin degradation products added to plasma were also unstable, although the sugar trehalose was found to improve stability. However, this preparation was not suitable as a standard for widely used assay platforms.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
Hajime Tajima Sakurai, Hidefumi Iwashita, Satoko Arakawa, Alifu Yikelamu, Mizuki Kusaba, Satoshi Kofuji, Hiroshi Nishina, Munetaka Ishiyama, Yuichiro Ueno, Shigeomi Shimizu
Summary: In this study, researchers developed a new method to monitor autophagic flux using small cell-permeable fluorescent probes. They successfully developed a red-fluorescent autophagic probe named DAPRed, which can recognize various autophagic vacuoles. By combining green- and red-fluorescent probes, they were able to easily detect autophagic flux.
Article
Biochemistry & Molecular Biology
Hannah E. Chia, Karl J. Koebke, Aathmaja A. Rangarajan, Nicole M. Koropatkin, E. Neil G. Marsh, Julie S. Biteen
Summary: A novel orange UnaG-ligand pair has been identified, which can be excited in the 532 nm green excitation microscopy channel and emits at 581 nm, showing high affinity for UnaG and a significant increase in fluorescence intensity and red shift in anoxic environments. This discovery expands the versatility of UnaG ligand-binding pocket for cell imaging at longer wavelengths.
ACS CHEMICAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Kaushik Bhattacharya, Didier Picard
Summary: The Hsp70 and Hsp90 molecular chaperone systems in eukaryotes are crucial for protein homeostasis under normal and stressed conditions, exhibiting both physical and functional interactions to maintain cellular proteostasis. Co-chaperones like Hop and Sti1 play a key role in facilitating substrate transfer from Hsp70 to Hsp90. Interestingly, while eukaryotes rely on the canonical Hsp70-Hop-Hsp90 ternary chaperone complex for optimal maturation and stability of specific clients, prokaryotes can form a binary chaperone complex without Hop, displaying enhanced protein folding and anti-aggregation activities.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biology
Cristina Batlle, Isabel Calvo, Valentin Iglesias, Cian J. Lynch, Marcos Gil-Garcia, Manuel Serrano, Salvador Ventura
Summary: Recent evidence suggests that coiled-coil (CC) regions overlapping with polyQ tracts play a critical role in amyloid formation and functional switch in human proteins. The human MED15 Mediator complex subunit forms homodimers in solution mediated by CC interactions, with MED15CC aggregating into amyloid fibrils.
COMMUNICATIONS BIOLOGY
(2021)
Article
Engineering, Biomedical
Marcos Gil-Garcia, Salvador Ventura
Summary: The study presents a novel approach to create highly functional, non-amyloid, and fluorescent inclusion bodies (IBs) using natural coiled-coil self-assembly properties. These protein-based nanoparticles effectively capture antibodies and are utilized for specific and simultaneous targeting of two unrelated cell types, potentially valuable in bioimaging and immunotherapy applications.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Marta Diaz-Caballero, Susanna Navarro, Salvador Ventura
Summary: The study utilized the biotin-streptavidin system to tailor the functionality of amyloid-like nanostructures, achieving diverse functionalization by decorating them with different streptavidin conjugates including gold nanoparticles, quantum dots, and enzymes. This approach not only enables the multifunctionalization of amyloid-like nanostructures but can also be used to build novel nano-biosensors.
Article
Biochemistry & Molecular Biology
Rosaria Gitto, Serena Vittorio, Federica Bucolo, Samuel Pena-Diaz, Rosalba Siracusa, Salvatore Cuzzocrea, Salvador Ventura, Rosanna Di Paola, Laura De Luca
Summary: Parkinson's disease is characterized by the death of dopaminergic neurons and the formation of intracellular proteinaceous accumulations. A compound named 15 has been identified to effectively prevent Parkinson's disease symptoms and affect the levels of related markers. Furthermore, these compounds also slightly reduce protein aggregation.
ACS CHEMICAL NEUROSCIENCE
(2022)
Review
Cell Biology
Samuel Pena-Diaz, Salvador Ventura
Summary: Parkinson's disease is the second most prevalent neurodegenerative disorder worldwide, characterized by the loss of dopaminergic neurons and the abnormal aggregation of alpha-synuclein. Some compounds with a single aromatic ring can act as molecular chaperones to inhibit alpha-synuclein aggregation and promote the formation of non-toxic oligomers.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Federica Quaglia, Balint Meszaros, Edoardo Salladini, Andras Hatos, Rita Pancsa, Lucia B. Chemes, Matyas Pajkos, Tamas Lazar, Samuel Pena-Diaz, Jaime Santos, Veronika Acs, Nazanin Farahi, Erzsebet Ficho, Maria Cristina Aspromonte, Claudio Bassot, Anastasia Chasapi, Norman E. Davey, Radoslav Davidovic, Laszlo Dobson, Arne Elofsson, Gabor Erdos, Pascale Gaudet, Michelle Giglio, Juliana Glavina, Javier Iserte, Valentin Iglesias, Zsofia Kalman, Matteo Lambrughi, Emanuela Leonardi, Sonia Longhi, Sandra Macedo-Ribeiro, Emiliano Maiani, Julia Marchetti, Cristina Marino-Buslje, Attila Meszaros, Alexander Miguel Monzon, Giovanni Minervini, Suvarna Nadendla, Juliet F. Nilsson, Marian Novotny, Christos A. Ouzounis, Nicolas Palopoli, Elena Papaleo, Pedro Jose Barbosa Pereira, Gabriele Pozzati, Vasilis J. Promponas, Jordi Pujols, Alma Carolina Sanchez Rocha, Martin Salas, Luciana Rodriguez Sawicki, Eva Schad, Aditi Shenoy, Tamas Szaniszlo, Konstantinos D. Tsirigos, Nevena Veljkovic, Gustavo Parisi, Salvador Ventura, Zsuzsanna Dosztanyi, Peter Tompa, Silvio C. E. Tosatto, Damiano Piovesan
Summary: The latest update of DisProt version 9 includes a restyled web interface, refactored IDPO, improvements in curation process, and around 30% content growth. The newly implemented reviewing process and curator training aim to provide higher quality and more consistent annotations. Integration with APICURON and adoption of MIADE standard, along with collaborations with GO and ECO consortia and support from ELIXIR infrastructure, enhance interoperability.
NUCLEIC ACIDS RESEARCH
(2022)
Editorial Material
Biochemistry & Molecular Biology
Jaime Santos, Irantzu Pallares, Salvador Ventura
Summary: alpha-Synuclein oligomers and fibrils play a role in neurodegeneration in Parkinson's disease. Amphipathic and cationic helical peptides can inhibit amyloid formation and neurotoxicity by binding to solvent-accessible regions in these toxic species. Can endogenous peptides like LL-37 be a new therapeutic approach in PD?
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Susanna Navarro, Salvador Ventura
Summary: Protein aggregation is associated with diseases and industrial production. This article reviews computational tools for predicting and identifying protein aggregation.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carlos Pintado-Grima, Oriol Barcenas, Salvador Ventura
Summary: This study conducted a large-scale in-silico analysis of the effect of solution pH on the solubility and disorder of intrinsically disordered regions (IDRs) involved in liquid-liquid phase separation (LLPS). The results showed that LLPS-DRs have maximum solubility around physiological pH, where LLPS often occurs, and significant differences were found between proteins that can phase-separate by themselves or those that require a partner.
Article
Biochemistry & Molecular Biology
Carlos Pintado-Grima, Oriol Barcenas, Zoe Manglano-Artunedo, Rita Vilaca, Sandra Macedo-Ribeiro, Irantzu Pallares, Jaime Santos, Salvador Ventura
Summary: Proteome-wide analyses have revealed that amyloidogenic regions are present in most globular proteins, while being underrepresented in intrinsically disordered proteins (IDPs). However, recent research has shown that intrinsically disordered regions (IDRs) do contain significant amyloid load in the form of cryptic amyloidogenic regions (CARs), which are exposed to solvent and are more polar than conventional amyloid regions. CARs are associated with both IDPs function and malfunction, and their presence is linked to pathologies such as cancer and Alzheimer's disease. To explore these previously unnoticed amyloidogenic regions, CARs-DB, a database containing precomputed predictions for all CARs in the IDPs deposited in the DisProt database, was developed. CARs-DB allows for easy access to a large number of unique CARs and has been validated by demonstrating the amyloidogenic potential of selected CARs.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Javier Garcia-Pardo, Andrea Bartolome-Nafria, Antonio Chaves-Sanjuan, Marcos Gil-Garcia, Cristina Visentin, Martino Bolognesi, Stefano Ricagno, Salvador Ventura
Summary: The study reports the cryo-electron microscopy structure of hnRNPDL-2 fibrils, revealing its stable, non-toxic, and nucleic acid-binding properties. The structure provides insights into the mechanism of hnRNPDL-2 fibrillation and its association with LGMD D3. Additionally, the study highlights how alternative splicing controls the assembly of different hnRNPDL isoforms, generating functional diversity.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Molood Behbahanipour, Roger Benoit, Susanna Navarro, Salvador Ventura
Summary: A novel nanoparticle has been developed that can neutralize SARS-CoV-2 virus. The nanoparticle is biocompatible and stable, and shows potential for therapeutic and diagnostic applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Susanna Navarro, Marta Diaz-Caballero, Francesca Peccati, Lorena Roldan-Martin, Mariona Sodupe, Salvador Ventura
Summary: Enzymes fold into specific 3D protein structures and exhibit high catalytic efficiency and selectivity. Artificial amyloids have been shown to have catalytic activity and advantages over natural enzymes. A recent study found that short peptides can self-assemble into amyloid fibrils with catalytic activity by coordinating and retaining different divalent metal cations. These findings provide new insights into the design of artificial metalloenzymes and support the role of amyloid-like structures in the origin of life.
News Item
Biochemistry & Molecular Biology
Javier Garcia-Pardo, Salvador Ventura
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra E. Badaczewska-Dawid, Aleksander Kuriata, Carlos Pintado-Grima, Javier Garcia-Pardo, Michal Burdukiewicz, Valentin Iglesias, Sebastian Kmiecik, Salvador Ventura
Summary: Protein aggregation is associated with aging and different pathologies, and is a challenge in the industrial production of biotherapeutics. Previous research in model organisms has provided insights into the biophysical principles of this process and led to the development of computational tools. A3D-MODB is a comprehensive database that allows for the study of protein aggregation in 12 model species and provides additional information for better understanding protein aggregation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)