Article
Biology
Holly Fowle, Ziran Zhao, Qifang Xu, Jason S. Wasserman, Xinru Wang, Mary Adeyemi, Felicity Feiser, Alison N. Kurimchak, Diba Atar, Brennan C. McEwan, Arminja N. Kettenbach, Rebecca Page, Wolfgang Peti, Roland L. Dunbrack, Xavier Grana
Summary: The study elucidates the mechanism by which PP2A/B55 alpha recognizes substrates and dephosphorylates them, identifying a key binding site in p107 and validating the necessity of related SLiM motif sequences for substrate binding.
Article
Biochemistry & Molecular Biology
Vanessa Gerlt, Juliane Mayr, Juliana Del Sarto, Stephan Ludwig, Yvonne Boergeling
Summary: The study demonstrates the importance of PP2A in efficient replication of various IAV subtypes, as decreased PP2Ac levels result in reduced cell viability and increased cell death after IAV infection. This is attributed to a synergistic action of hyper-activated PI3K/Akt, MAPK/JAK-STAT, and NF-kB signaling pathways related to apoptosis, indicating the critical role of PP2A in orchestrating cell survival mechanisms during IAV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Scott P. Lyons, Elora C. Greiner, Lauren E. Cressey, Mark E. Adamo, Arminja N. Kettenbach
Summary: The PPP family is responsible for cellular serine and threonine dephosphorylation, achieving substrate specificity and selectivity by forming multimeric holoenzymes. Methylation plays a crucial role in regulating PPP holoenzyme assembly for PP2A, PP4, and PP6, with implications in human diseases like cancer and neurodegeneration. Endogenous PPP methylation is shown to have a crucial regulatory function in transformed and non-transformed cell lines in an isoform-specific manner.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Mirabela Hali, Brian E. Wadzinski, Anjaneyulu Kowluru
Summary: This study investigated the role of Alpha4, a non-canonical subunit of protein phosphatase 2A, in the regulation of glucose-induced acute and chronic effects in pancreatic beta cells. Alpha4 expression was increased in response to high glucose and C2-Ceramide. Knockdown of Alpha4 attenuated ER stress and cell death induced by high glucose, while preventing the decrease in Connexin36 expression. These findings suggest that Alpha4 contributes to metabolic dysfunction and cell death in pancreatic beta cells under high glucose conditions.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Review
Immunology
Liang Ren, Daonan Shen, Chengcheng Liu, Yi Ding
Summary: The oral cavity contains a wide range of microbial species, and imbalanced microbiota-host interactions are responsible for various oral diseases. Recent studies have shown that bacterial protein phosphorylation plays a crucial role in oral bacterial dysbiosis and bacteria-host interactions, and ongoing research aims to uncover the underlying mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Karolina Pavic, Nikhil Gupta, Judit Domenech Omella, Rita Derua, Anna Aakula, Riikka Huhtaniemi, Juha A. Maatta, Nico Hofflin, Juha Okkeri, Zhizhi Wang, Otto Kauko, Roosa Varjus, Henrik Honkanen, Daniel Abankwa, Maja Kohn, Vesa P. Hytonen, Wenqing Xu, Jakob Nilsson, Rebecca Page, Veerle Janssens, Alexander Leitner, Jukka Westermarck
Summary: This study reveals the molecular level details and structural mechanisms of PP2A-B56 alpha inhibition by the oncoprotein CIP2A. CIP2A displaces the PP2A-A subunit and forms a pseudotrimer, blocking the B56 alpha substrate binding site and stabilizing CIP2A protein.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Alexander Y. Deneka, Meghan C. Kopp, Anna S. Nikonova, Anna Gaponova, Anna A. Kiseleva, Harvey H. Hensley, Douglas B. Flieder, Ilya G. Serebriiskii, Erica A. Golemis
Summary: This study reveals a new role for the scaffolding protein NEDD9 in regulating LKB1-AMPK signaling in early stage NSCLC, suppressing autophagy and tumor growth.
Review
Biochemistry & Molecular Biology
Patrick Partscht, Elmar Schiebel
Summary: CDC14 was initially identified as a crucial mediator of mitotic exit in budding yeast, belonging to the family of dual-specificity phosphatases (DUSPs) that are present in most eukaryotes. Contradicting data regarding the conservation of a cell cycle role in human paralogs CDC14A and CDC14B have sparked a contentious discussion, but redundancy may mask this role. Subsequent studies on CDC14A and CDC14B double knockouts in human and mouse have demonstrated that CDC14 activity is dispensable for mitotic progression in higher eukaryotes and instead suggested functional specialization.
Article
Biochemistry & Molecular Biology
Nikoleta G. Tsvetanova, Michelle Trester-Zedlitz, Billy W. Newton, Grace E. Peng, Jeffrey R. Johnson, David Jimenez-Morales, Andrew P. Kurland, Nevan J. Krogan, Mark von Zastrow
Summary: Endosomal signaling downstream of GPCRs plays a significant role in cellular responsiveness to cAMP, leading to distinct changes in phosphorylation reactions. A subset of proteins undergo dephosphorylation in response to cAMP, with compartmentalized PP2A activation by cAMP-responsive kinases as the likely mechanism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Immunology
Michal Slawomir Barski, Jordan James Minnell, Goedele Noella Maertens
Summary: Protein phosphatase 2A (PP2A) is a vital cellular protein that is targeted by many viruses for their own benefit, particularly oncogenic viruses. Recent research has revealed that various viruses hijack PP2A through molecular mimicry of a B56-specific motif, highlighting potential implications for therapeutic intervention.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Bernhard Hoermann, Maja Koehn
Summary: Phosphorylation of serine and threonine plays a crucial role in cell signaling, with the timely attachment, binding, and removal of phosphate being key factors in fast modulation of regulatory sites. The selection of phosphorylation sites is largely influenced by the sequence motif and the presence of regulatory proteins, while the context of substrate structure also affects recognition mechanisms. The interplay between kinases, pSer/pThr-binding proteins, and phosphatases at these crossroads of signaling motifs is essential for cellular adaptation to signals.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Medicine, Research & Experimental
Taishi Yamane, Yohei Kanamori, Hiroshi Sawayama, Hiromu Yano, Akihiro Nita, Yudai Ohta, Hironori Hinokuma, Ayato Maeda, Akiko Iwai, Takashi Matsumoto, Mayuko Shimoda, Mayumi Niimura, Shingo Usuki, Noriko Yasuda-Yoshihara, Masato Niwa, Yoshifumi Baba, Takatsugu Ishimoto, Yoshihiro Komohara, Tomohiro Sawa, Tasuku Hirayama, Hideo Baba, Toshiro Moroishi
Summary: Accumulating evidence suggests that high levels of Fusobacterium nucleatum in colorectal tumor tissues can be associated with poor prognosis in patients with colorectal cancer (CRC). This study shows that high-iron status and F. nucleatum-positive are both associated with worse prognosis. Iron plays a key role in modulating the NF-KB signaling pathway.
Article
Cell Biology
Min Liu, Aiguo Liu, Jie Wang, Yansong Zhang, Yajuan Li, Ying Su, Alan Jian Zhu
Summary: Protein phosphatase V (PpV) is identified as a homeostatic regulator of Hh signaling, which competes with the catalytic subunit of PP2A for Wdb association to negatively regulate Wdb stability. This competition ensures graded Hh signaling through regulated Wdb stability. PpV functions as a Hh activity sensor to maintain Hh signaling homeostasis by regulating Wdb-mediated PP2A activity through feedback mechanisms.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yumi Ueki, Michael A. Hadders, Melanie B. Weisser, Isha Nasa, Paula Sotelo-Parrilla, Lauren E. Cressey, Tanmay Gupta, Emil P. T. Hertz, Thomas Kruse, Guillermo Montoya, A. Arockia Jeyaprakash, Arminja Kettenbach, Susanne M. A. Lens, Jakob Nilsson
Summary: The study reveals the structure and function of the PP2A-B56-hSgo1 complex during mitosis, highlighting the essential role of a conserved pocket on the B56 regulatory subunit for hSgo1 binding and cohesion protection. Additionally, it shows that hSgo1 inhibits the binding of PP2A-B56 substrates and that PP2A-B56 dephosphorylates Cdk1 sites on hSgo1 to regulate cohesin interactions. Overall, the research provides important insights into cohesion protection during mitosis.
Article
Biology
S. Zachary Swartz, Hieu T. Nguyen, Brennan C. McEwan, Mark E. Adamo, Iain M. Cheeseman, Arminja N. Kettenbach
Summary: Through a proteomic study of sea star oocytes, the researchers identified dynamic waves of phosphorylation during meiosis, with the activation of phosphatase PP2A-B55 at the MI/MII transition playing a crucial role in specific phosphoregulated behaviors and distinguishing the two meiotic divisions. The study highlights the importance of selective dephosphorylation and substrate preferences in directing key cell cycle events during meiosis.
Review
Cell Biology
Peta Bradbury, Hanjie Wu, Jung Un Choi, Alan E. Rowan, Hongyu Zhang, Kate Poole, Jan Lauko, Joshua Chou
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Biology
Christoph Mark, Thomas J. Grundy, Pamela L. Strissel, David Boehringer, Nadine Grummel, Richard Gerum, Julian Steinwachs, Carolin C. Hack, Matthias W. Beckmann, Markus Eckstein, Reiner Strick, Geraldine M. O'Neill, Ben Fabry
Article
Pharmacology & Pharmacy
Hanieh Gholizadeh, Hui Xin Ong, Peta Bradbury, Agisilaos Kourmatzis, Daniela Traini, Paul Young, Ming Li, Shaokoon Cheng
Summary: The NEM-on-a-chip successfully mimics the functional characteristics of human nasal mucosa, monitors drug transport in real-time, and validates its quantification method. The study highlights the importance of conducting nasal drug transport studies under physiologically relevant dynamic conditions.
EXPERT OPINION ON DRUG DELIVERY
(2021)
Article
Pharmacology & Pharmacy
Zara Sheikh, Larissa Gomes Dos Reis, Peta Bradbury, Giulio Meneguzzo, Santo Scalia, Paul M. Young, Hui Xin Ong, Daniela Traini
Summary: The study suggests that Diclofenac could be a promising inhaled anti-inflammatory therapy for cystic fibrosis patients. Low dose Diclofenac formulations showed better aerosol performance and anti-inflammatory activity in vitro, highlighting their potential for CF treatment.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Louise Orcheston-Findlay, Samuel Bax, Robert Utama, Martin Engel, Dinisha Govender, Geraldine O'Neill
Summary: The life expectancy of patients with high-grade glioma (HGG) has not improved, highlighting the need for advanced models for future improvement. Currently, advanced models are crucial for identifying new targets and evaluating treatment modalities. While pediatric HGG (pHGG) models lag behind those of adults, there is hope to bring this to light and improve pGBM models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Zara Sheikh, Peta Bradbury, Tristan A. Reekie, Michele Pozzoli, Paul D. Robinson, Michael Kassiou, Paul M. Young, Hui Xin Ong, Daniela Traini
Summary: Current study investigated the potential interactions between the anti-inflammatory agent Ibuprofen and CF-approved inhaled antibiotics, revealing differential effects on the antibacterial and anti-inflammatory efficacy of the combinations. The study highlighted the importance of understanding drug-drug interactions and highlighted the inherent anti-inflammatory properties of the antibiotics used in CF treatment.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell Biology
Peta Bradbury, Aylin Cidem, Hadi Mahmodi, Janet M. Davies, Patrick T. Spicer, Stuart W. Prescott, Irina Kabakova, Hui Xin Ong, Daniela Traini
Summary: Pollen proteins can affect the structure and function of respiratory cells, leading to exacerbation of respiratory diseases. The study found that pollen proteins cause protein release and cytoskeleton reorganization in cells, resulting in the secretion of inflammatory factors and damage to the epithelial barrier.
Editorial Material
Hematology
Nicole M. Verrills
Summary: This study reveals a new role for the tumor suppressor protein phosphatase 2A in regulating differentiation and growth arrest in acute myeloid leukemia, and highlights the therapeutic potential of a specific drug.
Article
Materials Science, Multidisciplinary
Deepika Radhakrishnan, Shan Mohanan, Goeun Choi, Jin-Ho Choy, Steffi Tiburcius, Hoang Trung Trinh, Shankar Bolan, Nikki Verrills, Pradeep Tanwar, Ajay Karakoti, Ajayan Vinu
Summary: This review focuses on the use of nano-porous materials for drug delivery in lung cancer, analyzing the currently used materials and discussing the potential for future improvements. It provides a strong foundation for understanding the current research status and limitations, as well as the emerging trends in delivering drugs for the treatment of lung cancer.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2022)
Article
Multidisciplinary Sciences
Giulia Silvani, Peta Bradbury, Carin Basirun, Christine Mehner, Detina Zalli, Kate Poole, Joshua Chou
Summary: The study utilized an easy-to-use hybrid biological platform, combining 3D printing technology and PDMS microfluidic fabrication processes, to facilitate reliable microgravity cellular experiments. The results showed that microgravity has an impact on both cancer and healthy cell functionality, providing strategies and prospects for the development of brain cancer molecular therapies.
Article
Cell & Tissue Engineering
Ipek Karacan, Besim Ben-Nissan, Jerran Santos, Stanley Yiu, Peta Bradbury, Stella M. Valenzuela, Joshua Chou
Summary: The study aimed to improve current implant treatments by using a localized and controlled antibiotic delivery-based coating system. The results showed that the coating effectively prevented biofilm formation caused by Staphylococcus aureus and did not have adverse effects on human cells.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Biochemical Research Methods
Heather C. Murray, Kasey Miller, Joshua S. Brzozowski, Richard G. S. Kahl, Nathan D. Smith, Sean J. Humphrey, Matthew D. Dun, Nicole M. Verrills
Summary: Acute myeloid leukemia (AML) is a highly aggressive form of leukemia with a poor prognosis. Mutations in kinases, such as FLT3 and KIT, are common in AML patients and are associated with treatment resistance. This study identified DNA-PK as a potential therapeutic target in AML and demonstrated that DNA-PK inhibition sensitizes AML cells with FLT3 and KIT mutations to standard treatments. The findings suggest that targeting DNA-PK could improve the outcomes of AML patients with these mutations.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Review
Biotechnology & Applied Microbiology
Mathew N. Leslie, Joshua Chou, Paul M. Young, Daniela Traini, Peta Bradbury, Hui Xin Ong
Summary: Emphysema, the third leading cause of death worldwide, currently lacks effective treatments and is primarily caused by genetic factors or exposure to pollutants/irritants. Research suggests that the mechanical properties of the lung undergo significant changes in emphysema, and the role of fibroblasts in disease progression is crucial yet not fully understood.
BIOENGINEERING-BASEL
(2021)
Article
Pharmacology & Pharmacy
Giulia Silvani, Carin Basirun, Hanjie Wu, Christine Mehner, Kate Poole, Peta Bradbury, Joshua Chou
Summary: The study presents an innovative GBM-on-a-chip model that can recreate the brain tumor microenvironment and shows the significant role of gravitational forces in glioblastoma mechanical regulation under mechanical unloading conditions. This model is a meaningful biological tool for cancer mechanobiology research and pre-clinical approaches in brain tumor therapy.
ADVANCED THERAPEUTICS
(2021)
Article
Respiratory System
Peta Bradbury, Cassandra P. Nader, Aylin Cidem, Sandra Rutting, Dianne Sylvester, Patrick He, Maria C. Rezcallah, Geraldine M. O'Neill, Alaina J. Ammit
Summary: Many lung diseases are characterized by fibrosis, which is driven by the two-way interaction between cells and the extra-cellular matrix. The inflammatory cytokine growth factor beta(1) is believed to be a major driver of lung fibrosis.
RESPIRATORY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)