Article
Multidisciplinary Sciences
Yan Chen, Bin Tsai, Ningning Li, Ning Gao
Summary: The authors report structures of RAC-containing ribosomal complexes, providing insights into the dynamic actions of RAC-Ssb during co-translational folding.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Tianyu Zhao, Asaka Chida, Yuichi Shichino, Dongwoo Choi, Masaki Mizunuma, Shintaro Iwasaki, Yoshikazu Ohya
Summary: This study reveals the global translational regulation during replicative aging in yeast using ribosome profiling technique. The research found that the translational efficiency of genes involved in DNA repair and chromosome organization increased, while the translational efficiency of genes encoding ribosome components, transposons and cell wall assembly decreased during replicative aging.
Article
Biochemistry & Molecular Biology
Byung-Sik Shin, Ivaylo P. Ivanov, Joo-Ran Kim, Chune Cao, Terri G. Kinzy, Thomas E. Dever
Summary: Diphthamide (DPH) is a conserved amino acid modification on eukaryotic translation elongation factor eEF2. Loss of DPH impairs translational fidelity, leading to increased rates of ribosomal frameshifting and premature termination at out-of-frame stop codons. The ADP-ribosylation of DPH by bacterial toxins inhibits the binding of eEF2 to elongating ribosomes, further compromising translation accuracy. The costly, yet non-essential, DPH modification has been conserved through evolution to maintain translational fidelity.
NUCLEIC ACIDS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Agnes Baudin-Baillieu, Olivier Namy
Summary: Ribosomal RNA is a crucial component of ribosomes, playing a key role in peptide bond formation and accurate genetic code decoding. Recent research suggests that ribosomal RNA modifications are not 100% complete, leading to heterogeneous populations of ribosomes. Accumulated knowledge from yeast is valuable in understanding the role of ribosomal RNA modifications in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hongliang Zhang, Julia Quintana, Koray Uetkuer, Lorenz Adrian, Harmen Hawer, Klaus Mayer, Xiaodi Gong, Leonardo Castanedo, Anna Schulten, Nadezda Janina, Marcus Peters, Markus Wirtz, Ulrich Brinkmann, Raffael Schaffrath, Ute Kraemer
Summary: Diphthamide, a post-translational modification of eukaryotic translation elongation factor 2, is conserved in Arabidopsis thaliana and contributes to translational fidelity and growth by regulating cell proliferation. Diphthamide modification depends on specific proteins and its unmodification leads to increased ribosomal frameshifting-errors and decreased cell formation. Under abiotic stress, unmodified eEF2 accumulates in wild-type seedlings, suggesting its conservation across species.
NATURE COMMUNICATIONS
(2022)
Review
Microbiology
Ekaterina Samatova, Jan Daberger, Marija Liutkute, Marina V. Rodnina
Summary: Ribosome pausing affects translational efficiency and protein folding, as well as the quality of newly synthesized proteins. It emerges as an important factor in regulatory programs that maintain the quality of the proteome and integrate cellular and environmental cues into regulatory circuits of the cell.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Madeline G. Rollins, Manidip Shasmal, Nathan Meade, Helen Astar, Peter S. Shen, Derek Walsh
Summary: Poxviruses phosphorylate the ribosomal protein RACK1 in human hosts, altering ribosome structure and translation output to support noncanonical modes of translation and confer resistance to various protein synthesis inhibitors.
Article
Biochemistry & Molecular Biology
Huafeng Xu
Summary: Recent experimental studies have shown that ATP-driven molecular chaperones can stabilize protein substrates in their native structures out of thermal equilibrium. This article proposes a unifying principle based on available evidence, which explains the conversion of chemical energy from ATP hydrolysis to the conformational free energy associated with protein folding and activation.
Review
Microbiology
Rachael E. Turner, Traude H. Beilharz
Summary: Alternative polyadenylation (APA) plays a crucial role in regulating isoform-specific translation efficiency, stability, and localization. Despite advances in understanding APA in metazoans, its impact in Saccharomyces cerevisiae remains unclear. Experimental techniques are provided to uncover the functional importance of 3' UTR isoforms on translation, bridging the gap between observed APA and functional consequences.
Review
Biochemistry & Molecular Biology
Tom Joshua Eisenack, Debora Broch Trentini
Summary: Proteins are versatile molecular machines that control and execute cellular processes. They go through a multilayered synthesis process with many opportunities for error, and must navigate a complex folding-energy landscape. Newly synthesized proteins are at increased risk of misfolding and toxic aggregation, but cells employ molecular chaperones and quality control factors to maintain proteostasis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xabier Agirrezabala, Ekaterina Samatova, Meline Macher, Marija Liutkute, Manisankar Maiti, David Gil-Carton, Jiri Novacek, Mikel Valle, Marina Rodnina
Summary: Cellular proteins begin to fold as they emerge from the ribosome. The folding process is influenced by both the amino acid sequence and the interactions with the ribosome. Research shows that folding of a beta-barrel protein starts with the formation of a dynamic alpha-helix inside the ribosome, and the N-terminal part of the nascent chain refolds to a beta-hairpin structure before its release from the ribosome.
Article
Biotechnology & Applied Microbiology
Lu Meng, Xu Yang, Xue Lin, Huan-Yuan Jiang, Xiao-Ping Hu, Si-Xin Liu
Summary: The study found that overexpression of SNF1 in baker's yeast enhances cell tolerance and fermentation capacity during freezing by upregulating proteasome, altering carbon source and protectant molecule metabolism, and changing cell membrane components. Before freezing, SNF1 overexpression regulated levels of specific amino acids, glycerol, fatty acids, and phospholipids, while after freezing, it maintained high levels of certain metabolites contributing to freezing resistance. This research provides insights into cellular responses of baker's yeast to freezing and potential for developing low-temperature resistant strains.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Ailsa Black, Thomas D. Williams, Flavie Soubigou, Ifeoluwapo M. Joshua, Houjiang Zhou, Frederic Lamoliatte, Adrien Rousseau
Summary: This study reveals the essential role of the ribosome-associated complex (RAC)/Ssb chaperone system in maintaining proteostasis and cell viability under TORC1 inhibition. The absence of RAC/Ssb impairs translational control and protein degradation, leading to decreased cell survival in response to TORC1 inhibition.
Article
Biotechnology & Applied Microbiology
Xiaoyu Yang, Bo Song, Jie Cui, Lina Wang, Shuoshuo Wang, Linlin Luo, Lei Gao, Beixin Mo, Yu Yu, Lin Liu
Summary: This study reveals that translational reprogramming plays an important role in rice's response to salt stress, with the salt-tolerant cultivar SR86 adopting a more flexible translational adaptive strategy compared to the salt-sensitive cultivar NB. The differences in translational dynamics under salt stress between NB and SR86 may be due to their varying levels of ribosome stalling.
Review
Biochemistry & Molecular Biology
Panagiotis Poulis, Frank Peske, Marina V. Rodnina
Summary: During translation elongation, the ribosome moves along the mRNA by a codon with the assistance of elongation factor G (EF-G). This process involves precise structural rearrangements and maintains the correct codon-wise step size. However, mRNA signals and environmental cues can alter the timing and dynamics of these rearrangements, leading to recoding and the production of trans-frame peptides. In this review, recent advances in the mechanics of translocation and reading frame maintenance are discussed, as well as the mechanisms and biological relevance of non-canonical translocation pathways and their relationship to disease and infection.
BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Martina Tholen, Julia Wolanski, Britta Stolze, Marco Chiabudini, Mieczyslaw Gajda, Peter Bronsert, Elmar Stickeler, Sabine Rospert, Thomas Reinheckel
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Lena Boettinger, Silke Oeljeklaus, Bernard Guiard, Sabine Rospert, Bettina Warscheid, Thomas Becker
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Review
Genetics & Heredity
Volker Huebscher, Kaivalya Mudholkar, Sabine Rospert
Article
Biochemistry & Molecular Biology
Ying Zhang, Thea Schaeffer, Tina Woelfle, Edith Fitzke, Gerhard Thiel, Sabine Rospert
MOLECULAR AND CELLULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Volker Huebscher, Kaivalya Mudholkar, Marco Chiabudini, Edith Fitzke, Tina Woelfle, Dietmar Pfeifer, Friedel Drepper, Bettina Warscheid, Sabine Rospert
NUCLEIC ACIDS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Andrea Gumiero, Charlotte Conz, Genis Valentin Gese, Ying Zhang, Felix Alexander Weyer, Karine Lapouge, Julia Kappes, Ulrike von Plehwe, Geza Schermann, Edith Fitzke, Tina Woelfle, Tamas Fischer, Sabine Rospert, Irmgard Sinning
NATURE COMMUNICATIONS
(2016)
Article
Microbiology
Alexander Belyy, Nadezhda Levanova, Irina Tabakova, Sabine Rospert, Yury Belyi
Article
Biochemistry & Molecular Biology
Thomas Jank, Yury Belyi, Christophe Wirth, Sabine Rospert, Zehan Hu, Joern Dengjel, Tina Tzivelekidis, Gregers Rom Andersen, Carola Hunte, Andreas Schlosser, Klaus Aktories
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Review
Biochemistry & Molecular Biology
Ying Zhang, Irmgard Sinning, Sabine Rospert
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Review
Biochemistry & Molecular Biology
Ying Zhang, Irmgard Sinning, Sabine Rospert
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Review
Cell Biology
Harm H. Kampinga, Claes Andreasson, Alessandro Barducci, Michael E. Cheetham, Douglas Cyr, Cecilia Emanuelsson, Pierre Genevaux, Jason E. Gestwicki, Pierre Goloubinoff, Jaime Huerta-Cepas, Janine Kirstein, Krzysztof Liberek, Matthias P. Mayer, Kazuhiro Nagata, Nadinath B. Nillegoda, Pablo Pulido, Carlos Ramos, Paolo De los Rios, Sabine Rospert, Rina Rosenzweig, Chandan Sahi, Mikko Taipale, Bratlomiej Tomiczek, Ryo Ushioda, Jason C. Young, Richard Zimmermann, Alicja Zylicz, Maciej Zylicz, Elizabeth A. Craig, Jaroslaw Marszalek
CELL STRESS & CHAPERONES
(2019)
Article
Cell Biology
Daniel J. Puleston, Michael D. Buck, Ramon I. Klein Geltink, Ryan L. Kyle, George Caputa, David OSullivan, Alanna M. Cameron, Angela Castoldi, Yaarub Musa, Agnieszka M. Kabat, Ying Zhang, Lea J. Flachsmann, Cameron S. Field, Annette E. Patterson, Stefanie Scherer, Francesca Alfei, Francesc Baixauli, S. Kyle Austin, Beth Kelly, Mai Matsushita, Jonathan D. Curtis, Katarzyna M. Grzes, Matteo Villa, Mauro Corrado, David E. Sanin, Jing Qiu, Nora Pallman, Katelyn Paz, Maria Elena Maccari, Bruce R. Blazar, Gerhard Mittler, Joerg M. Buescher, Dietmar Zehn, Sabine Rospert, Edward J. Pearce, Stefan Balabanov, Erika L. Pearce
Article
Biochemistry & Molecular Biology
Anne-Sophie Gribling-Burrer, Marco Chiabudini, Ying Zhang, Zonghao Qiu, Mario Scazzari, Tina Woelfle, Daniel Wohlwend, Sabine Rospert
NUCLEIC ACIDS RESEARCH
(2019)
Article
Multidisciplinary Sciences
Ying Zhang, Genis Valentin Gese, Charlotte Conz, Karine Lapouge, Juergen Kopp, Tina Woelfle, Sabine Rospert, Irmgard Sinning
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Ying Zhang, Evelina De Laurentiis, Katherine E. Bohnsack, Mascha Wahlig, Namit Ranjan, Simon Gruseck, Philipp Hackert, Tina Wolfle, Marina Rodnina, Blanche Schwappach, Sabine Rospert
Summary: The GET pathway aids in delivering tail-anchored proteins to the ER, with Get4/5 components probing near the ribosomal exit tunnel and recruiting Sgt2 upon emergence of client proteins to initiate the targeting phase of the pathway.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)