Article
Biochemistry & Molecular Biology
Abramo J. Manfredonia, Daniel A. Kraut
Summary: The ubiquitin-proteasome system is responsible for protein degradation in eukaryotic cells. The study showed that degradation of ubiquitin-independent degrons (UbIDs) is slower and relies on loosely folded substrates. Furthermore, UbID degradation is ATP-independent.
Review
Biochemistry & Molecular Biology
Carolyn Allain Breckel, Mark Hochstrasser
Summary: The proper folding of proteins is vital for their diverse functions, and misfolded proteins can potentially harm cells by forming aggregates. Protein quality control pathways are responsible for repairing or degrading abnormal proteins, with the ubiquitin-proteasome system being commonly employed.
Article
Multidisciplinary Sciences
Afu Fu, Victoria Cohen-Kaplan, Noa Avni, Ido Livneh, Aaron Ciechanover
Summary: The degradation of proteins through the ubiquitin-proteasome system is a complex multistep process that relies on the coordinated activity of various enzymes. Nuclear condensates containing essential components like p62 play a crucial role in protein quality control and degradation, especially under stress conditions. These assemblies, generated through liquid-liquid phase separation, efficiently facilitate proteolysis of nuclear proteins and unassembled proteasome subunits, indicating their involvement in cellular protein quality control.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Hangjun Sun, Xinxin Jing, Chaonan Wang, Pengyue Wang, Ziting Huang, Bingjian Sun, Pengbai Li, Honglian Li, Chao Zhang
Summary: Plant viruses cause significant damage to global crop production and plants activate defense signaling pathways to hinder virus propagation. Protein homeostasis regulation plays a vital role in the ongoing battle between plants and viruses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yanhui Zhou, Hakim Manghwar, Weiming Hu, Fen Liu
Summary: Autophagy is a key pathway for nutrient recycling in eukaryotes, influenced by various factors such as hormones, second messengers, post-transcriptional regulation, and protein post-translational modification. It is activated under stress conditions to help cells survive, and the degradation mechanism of autophagy-related proteins in different organisms has attracted attention.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jennifer Cable, Eilika Weber-Ban, Tim Clausen, Kylie J. Walters, Michal Sharon, Daniel J. Finley, Yangnan Gu, John Hanna, Yue Feng, Sascha Martens, Anne Simonsen, Malene Hansen, Hong Zhang, Jonathan M. Goodwin, Alessio Reggio, Chunmei Chang, Liang Ge, Brenda A. Schulman, Raymond J. Deshaies, Ivan Dikic, J. Wade Harper, Ingrid E. Wertz, Nicolas H. Thoma, Mikolaj Slabicki, Judith Frydman, Ursula Jakob, Della C. David, Eric J. Bennett, Carolyn R. Bertozzi, Richa Sardana, Vinay V. Eapen, Serena Carra
Summary: Targeted protein degradation is essential for cellular function and development. This process involves tightly regulated protein degradation pathways to eliminate misfolded and aggregated proteins, adjust protein levels during cellular differentiation, and selectively eliminate target proteins. Understanding these pathways can provide insights into disease pathology and the development of novel therapeutics.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Review
Cell Biology
Yosup Kim, Eun-Kyung Kim, Yoona Chey, Min-Jeong Song, Ho Hee Jang
Summary: The proteasome is a multi-catalytic protease complex that regulates protein quality control. It selectively degrades cellular proteins through ubiquitination and plays a critical role in maintaining protein homeostasis. This article summarizes the proteasome's structure, regulatory mechanisms, proteins that control its activity, and its involvement in various diseases, chemoresistant cells, and cancer stem cells. Potential therapeutic modalities that target the ubiquitin-proteasome system are also discussed.
Article
Biochemistry & Molecular Biology
Caroline Kampmeyer, Martin Gronbaek-Thygesen, Nicole Oelerich, Michael H. Tatham, Matteo Cagiada, Kresten Lindorff-Larsen, Wouter Boomsma, Kay Hofmann, Rasmus Hartmann-Petersen
Summary: Lysine is a common amino acid in the human proteome, but there are proteins that lack lysine residues. These lysine deserts are common in intrinsically disordered proteins involved in the ubiquitin-proteasome system. Introducing lysine residues can increase ubiquitylation of these proteins, and their stability and function may be affected. This avoidance of lysine residues may be an evolutionary mechanism to prevent unnecessary ubiquitylation in proteins closely involved with the ubiquitylation machinery.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Justin B. Gregor, Dantong Xu, Michael E. French
Summary: Protein ubiquitylation is a crucial modification that regulates various aspects of eukaryotic cell biology. Ubiquitin chains can be branched and this branching directly affects the stability or activity of the target proteins. This review discusses the mechanisms controlling the assembly and disassembly of branched chains by ubiquitylation enzymes and deubiquitylases. It also summarizes the activities of chain branching ubiquitin ligases and deubiquitylases responsible for cleaving branched chains, as well as recent findings on the formation of branched chains and selective debranching by UCH37.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Pharmacology & Pharmacy
Si-Min Qi, Jinyun Dong, Zhi-Yuan Xu, Xiang-Dong Cheng, Wei-Dong Zhang, Jiang-Jiang Qin
Summary: PROTAC technology is an effective method for degrading target proteins through the ubiquitin-proteasome system, with promising applications in cancer treatment. The first oral PROTACs have shown encouraging results in clinical trials, sparking greater enthusiasm for PROTAC research.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cell Biology
Michael Basler, Marcus Groettrup
Summary: The immunoproteasome is a special type of proteasome induced under inflammatory conditions, contributing to protein homeostasis in cells and involved in immune response, T cell expansion, and inflammatory diseases. Targeting the immunoproteasome has shown therapeutic effectiveness in cancer, autoimmune diseases, and transplantation in preclinical animal models.
Article
Cell Biology
Nikol Dibus, Vladimir Korinek, Lukas Cermak
Summary: The gene encoding FBXO38, an E3 ubiquitin ligase substrate receptor, has been found to be associated with various diseases. The study shows that FBXO38 controls the stability of ZXDA/B proteins through ubiquitination and proteasome-dependent degradation. These ZXDA/B proteins are involved in the regulation of chromatin-associated CENP-B protein levels, and their inappropriate stabilization leads to upregulation of CENP-A and CENP-B in centromeric chromatin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tanggang Deng, Qianling Zhu, Lin Xie, Youhong Liu, Yuchong Peng, Linglong Yin, Yingxue Gao, Tuoyu Cao, Yuxin Fu, Xuli Qi, Songwei Zhang, Yongbo Peng, Youxiang Hou, Xiong Li
Summary: The cell division cycle (CDC6) has been identified as a critical molecule contributing to radioresistance in cancer cells. The demethylated form of cantharidin, Norcantharidin (NCTD), has been found to re-sensitize radioresistant cancer cells by inducing CDC6 protein degradation, leading to enhanced cell killing and apoptosis. NCTD promotes CDC6 protein degradation through a Cullin1 neddylation-mediated ubiquitin-proteasome pathway.
MOLECULAR CARCINOGENESIS
(2022)
Article
Cell Biology
Yueh-Ling Pai, Yuchieh Jay Lin, Wen-Hsin Peng, Li-Ting Huang, He-Yen Chou, Chien-Hsiang Wang, Cheng-Ting Chien, Guang-Chao Chen
Summary: This study reveals that Leon/USP5 is a DUB involved in the regulation of autophagy, interacting with Atg1/ULK1 and negatively regulating the autophagic process.
CELL DEATH & DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Xinyi Li, Wenchen Pu, Qingquan Zheng, Min Ai, Song Chen, Yong Peng
Summary: PROTAC is an engineered technique for targeted protein degradation, which recruits target protein and E3 ubiquitin ligase to trigger the degradation of target protein. It has great potential in cancer therapy and offers advantages over traditional anti-cancer therapies.
Article
Peripheral Vascular Disease
Mudi Misgav, Ahron Lubetszki, Tami Brutman-Barazani, Uri Martinowitz, Gili Kenet
JOURNAL OF VASCULAR ACCESS
(2017)
Article
Biochemistry & Molecular Biology
Dov Kesten, Miriam Horovitz-Fried, Tamar Brutman-Barazani, Sanford R. Sampson
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2018)
Article
Oncology
Assaf A. Barg, Einat Avishai, Ivan Budnik, Sarina Levy-Mendelovich, Tami B. Barazani, Gili Kenet, Tami Livnat
PEDIATRIC BLOOD & CANCER
(2019)
Article
Hematology
Sarina Levy-Mendelovich, Tami Livnat, Assaf Arie Barg, Mona Kidon, Tami Brutman-Barazani, Gili Kenet
BLOOD CELLS MOLECULES AND DISEASES
(2020)
Article
Hematology
Assaf A. Barg, Tami Livnat, Ivan Budnik, Einat Avishai, Tami Brutman-Barazani, Ilia Tamarin, Dalia Bashari, Mudi Misgav, Gili Kenet
BRITISH JOURNAL OF HAEMATOLOGY
(2020)
Article
Hematology
Assaf A. Barg, Einat Avishai, Ivan Budnik, Tamar Barazani Brutman, Ilia Tamarin, Rima Dardik, Dalia Bashari, Mudi Misgav, Aharon Lubetsky, Shadan Lalezari, Tami Livnat, Gili Kenet
Summary: This study assessed the potential role of Emicizumab as an alternative prophylactic treatment for severe VWD using ex vivo thrombin generation analysis. The results showed that spiking with Emicizumab increased thrombin generation in plasma from patients with type 3 VWD. The therapy was well tolerated and provided sufficient hemostasis without exceeding healthy levels, suggesting further collaborative studies on its efficacy and safety are warranted.
BLOOD CELLS MOLECULES AND DISEASES
(2021)
Article
Hematology
Mudi Misgav, Tami Brutman-Barazani, Ivan Budnik, Avishai Einat, Jonathan Schapiro, Dalia Bashari, Assaf A. Barg, Aaron Lubetsky, Tami Livnat, Gili Kenet
Summary: This study evaluated the safety and efficacy of Emicizumab in elderly Haemophilia A patients over 50 years old, showing a significant decrease in bleeding rates post-treatment and suggesting potential value in monitoring laboratory assays in this age group.
Article
Hematology
Assaf A. Barg, Ivan Budnik, Einat Avishai, Tami Brutman-Barazani, Dalia Bashari, Mudi Misgav, Aaron Lubetsky, Amir A. Kuperman, Tami Livnat, Gili Kenet
Summary: A study on the efficacy and safety of long-term prophylaxis with emicizumab in patients with severe haemophilia A found that most patients tolerated the treatment well, although some experienced breakthrough bleeding. Further studies are needed to explore the potential benefits in specific patient populations.
Article
Oncology
Assaf Arie Barg, Sarina Levy-Mendelovich, Ivan Budnik, Noa Mandel-Shorer, Rima Dardik, Einat Avishai, Tami Brutman-Barazani, Aviya Dvir Ifrah, Liat Oren-Malek, Joanne Yacobovich, Oded Gilad, Sigal Nakav, Yariv Fruchtman, Shoshana Revel-Vilk, Hagit Miskin, Gili Kenet
Summary: This study examined medical records of 60 children with FXI deficiency and found that proper hemostasis management during surgeries is crucial, also suggesting an association between the severity of FXI deficiency and bleeding tendency in pediatric patients.
PEDIATRIC BLOOD & CANCER
(2022)
Article
Medicine, General & Internal
Sarina Levy-Mendelovich, Tami Brutman-Barazani, Ivan Budnik, Einat Avishai, Assaf A. Barg, Tamara Levy, Mudi Misgav, Tami Livnat, Gili Kenet
Summary: Despite treatment with emicizumab, the risk of bleeding persists, especially in older patients. The occurrence of spontaneous and traumatic bleeding did not significantly differ at different time points.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Assaf A. Barg, Tami Brutman-Barazani, Einat Avishai, Ivan Budnik, Omri Cohen, Rima Dardik, Sarina Levy-Mendelovich, Tami Livnat, Gili Kenet
Summary: This study assessed the potential impact of Marstacimab on thrombin generation in plasma samples from patients with rare bleeding disorders (RBD). The results showed that the addition of Marstacimab improved certain coagulation parameters. These findings suggest that Marstacimab may be a promising treatment approach for restoring hemostatic balance in various RBDs.
BLOOD CELLS MOLECULES AND DISEASES
(2022)
Article
Hematology
Omri Cohen, Nitsan Landau, Einat Avishai, Tami Brutman-Barazani, Ivan Budnik, Tami Livnat, Keren Asraf, Ram Doolman, Sarina Levy-Mendelovich, Orly Efros, Uri Manor, Eyal Meltzer, Gad Segal, Galia Rahav, Gili Kenet
Summary: This study analyzed the correlation between thrombin generation (TG) capacity in COVID-19 patients and disease severity and clinical outcomes. The results showed that TG was not associated with disease severity, but endogenous thrombin potential (ETP) correlated with sepsis-induced coagulopathy (SIC) score. This finding is worth noting.
ACTA HAEMATOLOGICA
(2023)
Article
Oncology
Omri Cohen, Kfir Lange, Ivan Budnik, Ilia Tamarin, Tami Brutman-Barazani, Assaf Arie Barg, Nurit Rosenberg, Aharon Lubetsky, Gili Kenet, Sarina Levy-Mendelovich
Summary: There are differences in clinical decision tool scores, clinical characteristics, and laboratory findings between pediatric and adult patients with suspected HIT. Children have higher 4Ts scores compared to adults, potentially leading to a higher incidence of thrombosis in HIT pediatric patients due to the severity of underlying illness.
PEDIATRIC BLOOD & CANCER
(2022)
Article
Hematology
Tami Livnat, Ivan Budnik, Sarina Levy-Mendelovich, Einat Avishai, Mudi Misgav, Assaf Arie Barg, Aharon Lubetsky, Tami Brutman-Barazani, Gili Kenet
BLOOD CELLS MOLECULES AND DISEASES
(2017)
Article
Dermatology
Isaac Zilinsky, Tamar Brutman Barazani, Boris Shenkman, Oren Weisman, Nimrod Farber, Uriel Martinowitz
JOURNAL OF DRUGS IN DERMATOLOGY
(2016)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)