Article
Biochemistry & Molecular Biology
Tengfei Zhang, Shuai Jiang, Li Sun
Summary: In the study, a tandem repeat galectin-8 (CsGal-8) was identified and characterized in tongue sole, showing binding and bactericidal effects against a wide range of bacteria. CsGal-8 likely plays a crucial role in immune defense by interacting with and killing bacterial pathogens directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Jiayang Liu, Danjie Pan, Xuan Huang, Songna Wang, Huaning Chen, Yi Zhun Zhu, Li Ye
Summary: This review summarizes the methods and applications of targeting collagen in the tumor extracellular matrix, as well as the potential improvements in tumor-targeting immunotherapy. Collagen accumulates in tumor stroma and beneath tumor blood vessel endothelium, and targeting collagen allows immunotherapy drugs to accumulate in the tumor and enhance their efficacy, while avoiding severe adverse effects.
FRONTIERS IN ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Matheus V. F. Ferraz, Emerson G. Moreira, Danilo F. Coelho, Gabriel L. Wallau, Roberto D. Lins
Summary: Immune evasion of SARS-CoV-2 variants is primarily attributed to decreased cross-reactivity of previously elicited antibodies, with increased affinity to hACE2 playing a smaller role. The affinity between antibodies and variants is influenced by the remodeling of electrostatic surface potential of the Spike RBDs. The P.3 variant is considered a putative VOC.
CHEMICAL COMMUNICATIONS
(2021)
Review
Microbiology
William T. Harvey, Alessandro M. Carabelli, Ben Jackson, Ravindra K. Gupta, Emma C. Thomson, Ewan M. Harrison, Catherine Ludden, Richard Reeve, Andrew Rambaut, Sharon J. Peacock, David L. Robertson
Summary: The evolution of SARS-CoV-2 has been characterized by the emergence of mutations and variants that impact virus characteristics. Manufacturers are preparing for possible updates to vaccines in response to changes in the virus population, and it is crucial to monitor genetic and antigenic changes alongside experiments to understand the impacts of mutations.
NATURE REVIEWS MICROBIOLOGY
(2021)
Article
Biology
Djordje Atanackovic, Stephanie Avila, Forat Lutfi, Diego de Miguel-Perez, Xiaoxuan Fan, Gabriela Sanchez-Petitto, Erica Vander Mause, Jonathan Siglin, John Baddley, Heather D. Mannuel, Hanan Alkhaldi, Kim G. Hankey, Rena Lapidus, Michael Kleinberg, Joseph Rabin, Carl Shanholtz, Christian Rolfo, Aaron P. Rapoport, Saurabh Dahiya, Tim Luetkens
Summary: The study screened COVID-19 patients for antibodies against 9 different SARS-CoV-2 proteins, identifying immune responses in high-risk patients with significantly higher levels of certain antibodies and transforming growth factor beta, as well as lower levels of specific T cell expression. These findings aid in understanding humoral immune responses against SARS-CoV-2 variants and can assist in designing more effective immunomonitoring methods for COVID-19 vaccines.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhikuan Zhang, Takuma Shibata, Akiko Fujimura, Jiro Kitaura, Kensuke Miyake, Umeharu Ohto, Toshiyuki Shimizu
Summary: This study identifies thioredoxin (TRX) as a binder of the inflammasome sensor NLRP1 and a suppressor of NLRP1 inflammasome activity. The oxidized form of TRX binds to the nucleotide-binding domain subdomain of NLRP1, highlighting the essential role of redox-active cysteines of TRX in NLRP1 binding. The findings suggest that the TRX system acts as an intrinsic checkpoint for innate immunity and provides potential targets for future therapeutic intervention in NLRP1 inflammasome activation.
Review
Chemistry, Medicinal
Nerella Sridhar Goud, Ahana Bhattacharya
Summary: Gal-1, a carbohydrate-binding protein found in humans, plays a crucial role in tumor progression in various cancers, affecting processes such as tumor transformation, cell cycle regulation, apoptosis, cell adhesion, migration, and inflammation. Its interaction with carbohydrates on cell-surface, cytosol, and nucleus regulates cellular functions, making it a promising molecular target for new therapeutic tools.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2021)
Article
Immunology
Juliane Guenther, Sebastian Peter Galuska
Summary: Galectins are a diverse family of carbohydrate-binding proteins found in vertebrates, and their evolutionary history has been revealed through genomic analyses. The study highlights the origin and evolution of galectin family members in different animal groups, including primitive Bilateria and jawed vertebrates. Additionally, the research identifies a previously neglected galectin-related protein in Gnathostomata.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Anais Dabbadie, Anna Salerno, Adam Perriman, Lu-Yun Lian, Anthony P. Hollander
Summary: This study found that the three modules of the collagen binding domain (CBD) of matrix metalloproteinase-2 (MMP-2) have specific biological properties that can be exploited for targeted stem cell therapy of cartilage lesions and other pathological sites.
Article
Engineering, Biomedical
Xingzhi Liu, Lulu Zhang, Zhongjuan Xu, Xuan Xiong, Yanzhen Yu, Hanfei Wu, Hong Qiao, Junjie Zhong, Zhe Zhao, Jianwu Dai, Guangli Suo
Summary: This study aims to develop genetically engineered miR21-loaded exosomes that can be stably tethered to a collagen-I scaffold, promoting the sustained release of miR21 and benefiting spinal cord injury repair.
ACTA BIOMATERIALIA
(2022)
Review
Pharmacology & Pharmacy
Selena Bouffette, Iuliana Botez, Frederic De Ceuninck
Summary: This article reviews the key role of Galectin-3 (Gal-3) in cardiac, hepatic, renal, and pulmonary fibrosis and inflammation, respiratory infections caused by COVID-19, and neuroinflammatory disorders. The recent strategic breakthroughs have led to the identification of new-generation Gal-3 inhibitors with improved potency, selectivity, and bioavailability, which have shown promise as valuable tools in proof-of-concept studies in preclinical models of these diseases, particularly those in clinical stages. Critical views and suggestions to expand the therapeutic opportunities provided by this complex target are also discussed.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yan Wang, Hai-Nan Su, Hai-Yan Cao, Si-Min Liu, Shi-Cheng Liu, Xia Zhang, Peng Wang, Chun-Yang Li, Yu-Zhong Zhang, Xi-Ying Zhang, Xiu-Lan Chen
Summary: Collagenases produced by pathogenic Vibrio species are important virulence factors. This study characterizes the M9A collagenase VP397 from Marine Vibrio pomeroyi strain 12613 and reveals its degradation mechanism on type I collagen. VP397 first attacks the C-telopeptide region and then digests tropocollagen fragments at the Y-Gly bonds. This study provides new knowledge about the collagenolytic mechanism of Vibrio collagenase, which has implications for understanding Vibrio pathogenesis and developing industrial and medical applications of collagenase.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Engineering, Biomedical
Colin Logie, Tom van Schaik, Tilo Pompe, Katja Pietsch
Summary: The study found that fibronectin induces macrophage tolerance instead of M1 or M2 macrophage phenotypes during the differentiation from monocytes to macrophages. Immune suppression caused by fibronectin was overridden upon LPS challenge, allowing the macrophages to deploy an efficient immune response.
Article
Multidisciplinary Sciences
Amin Addetia, Luca Piccoli, James Brett Case, Young-Jun Park, Martina Beltramello, Barbara Guarino, Ha Dang, Guilherme Dias de Melo, Dora Pinto, Kaitlin Sprouse, Suzanne M. Scheaffer, Jessica Bassi, Chiara Silacci-Fregni, Francesco Muoio, Marco Dini, Lucia Vincenzetti, Rima Acosta, Daisy Johnson, Sambhavi Subramanian, Christian Saliba, Martina Giurdanella, Gloria Lombardo, Giada Leoni, Katja Culap, Carley Mcalister, Anushka Rajesh, Exequiel Dellota, Jiayi Zhou, Nisar Farhat, Dana Bohan, Julia Noack, Alex Chen, Florian A. Lempp, Joel Quispe, Lauriane Kergoat, Florence Larrous, Elisabetta Cameroni, Bradley Whitener, Olivier Giannini, Pietro Cippa, Alessandro Ceschi, Paolo Ferrari, Alessandra Franzetti-Pellanda, Maira Biggiogero, Christian Garzoni, Stephanie Zappi, Luca Bernasconi, Min Jeong Kim, Laura E. Rosen, Gretja Schnell, Nadine Czudnochowski, Fabio Benigni, Nicholas Franko, Jennifer K. Logue, Courtney Yoshiyama, Cameron Stewart, Helen Chu, Herve Bourhy, Michael A. Schmid, Lisa A. Purcell, Gyorgy Snell, Antonio Lanzavecchia, Michael S. Diamond, Davide Corti, David Veesler
Summary: The recently emerged BQ.1.1 and XBB.1.5 variants of SARS-CoV-2 have a higher affinity for the host ACE2 receptor and more efficiently promote fusion with host cell membranes compared to earlier Omicron variants. Although the neutralizing activity is reduced, vaccine-induced human plasma antibodies still cross-react with and trigger effector functions against current Omicron variants, providing a mechanism of protection against disease.
Article
Oncology
Samantha B. Kemp, Eileen S. Carpenter, Nina G. Steele, Katelyn L. Donahue, Zeribe C. Nwosu, Amanda Pacheco, Ashley Velez-Delgado, Rosa E. Menjivar, Fatima Lima, Stephanie The, Carlos E. Espinoza, Kristee Brown, Daniel Long, Costas A. Lyssiotis, Arvind Rao, Yaqing Zhang, Marina Pasca di Magliano, Howard C. Crawford
Summary: Elevated levels of apolipoprotein E in PDAC are associated with immune suppression and poor patient survival. ApoE induces expression of immunosuppressive factors in pancreatic tumor cells, potentially playing a novel immunosuppressive role in the PDAC microenvironment.
Article
Biochemistry & Molecular Biology
Shin-ichi Nakakita, Aiko Itoh, Yukari Nakakita, Yasuhiro Nonaka, Takashi Ogawa, Takanori Nakamura, Nozomu Nishi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Cell & Tissue Engineering
Kentaro Uchida, Osamu Matsushita, Nozomu Nishi, Gen Inoue, Kyosuke Horikawa, Masashi Takaso
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2017)
Article
Anatomy & Morphology
Ryuji Fujihara, Yoichi Chiba, Toshitaka Nakagawa, Nozomu Nishi, Ryuta Murakami, Koichi Matsumoto, Machi Kawauchi, Tetsuji Yamamoto, Masaki Ueno
MICROSCOPY RESEARCH AND TECHNIQUE
(2016)
Article
Clinical Neurology
Gen Inoue, Kentaro Uchida, Osamu Matsushita, Hisako Fujimaki, Wataru Saito, Masayuki Miyagi, Hiroyuki Sekiguchi, Nozomu Nishi, Seiji Ohtori, Mizuki Yogoro, Masashi Takaso
Article
Biotechnology & Applied Microbiology
Hiroyuki Sekiguchi, Kentaro Uchida, Osamu Matsushita, Gen Inoue, Nozomu Nishi, Ryo Masuda, Nana Hamamoto, Takaki Koide, Shintaro Shoji, Masashi Takaso
BIOMED RESEARCH INTERNATIONAL
(2018)
Article
Biochemistry & Molecular Biology
Aiko Itoh, Yasuhiro Nonaka, Shin-ichi Nakakita, Hiromi Yoshida, Nozomu Nishi, Takanori Nakamura, Shigehiro Kamitori
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Yasuhiro Nonaka, Takashi Ogawa, Hiroki Shoji, Nozomu Nishi, Shigehiro Kamitori, Takanori Nakamura
Summary: This study elucidated the key residues for carbohydrate specificity of Xenopus laevis skin galectin xgalectin-Va through site-directed mutagenesis, leading to a successful change in carbohydrate selectivity. The mutation of two amino acids greatly enhanced the binding ability to N-acetyllactosamine and conferred significant T-cell growth inhibition activity. The loop substitution also successfully altered the carbohydrate selectivity of xgalectin-Va and xgalectin-Ib.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Yasuhiro Nonaka, Takashi Ogawa, Hiroki Shoji, Nozomu Nishi, Shigehiro Kamitori, Takanori Nakamura
Summary: Modification of galectins' domain architecture, such as the tandem-repeat mutant with a short linker peptide, retained the native structure, showed higher T-cell growth-inhibition activity, and prevented oxidation of internal cysteine residue.
Article
Biochemistry & Molecular Biology
Aiko Itoh, Yasuhiro Nonaka, Takashi Ogawa, Takanori Nakamura, Nozomu Nishi
Article
Biochemistry & Molecular Biology
Aiko Itoh, Yasuhiro Nonaka, Takashi Ogawa, Takanori Nakamura, Nozomu Nishi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Hiromi Yoshida, Nozomu Nishi, Kenji Wada, Takanori Nakamura, Mitsuomi Hirashima, Naoyuki Kuwabara, Ryuichi Kato, Shigehiro Kamitori
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)