Article
Biochemistry & Molecular Biology
Hsiang-Pu Feng, Yu -Chin Liu, Chih-Liang Wang, Wei -Chao Liao, Jau-Song Yu, Chia-Jung Yu
Summary: KPNA2 is a nucleoplasmic protein responsible for nuclear import. Aberrant nuclear accumulation of KPNA2 has been observed in cancer tissues. AMPK is involved in the phosphorylation and acetylation of KPNA2, but its impact on the nucleocytoplasmic distribution of KPNA2 and its oncogenic role is unclear.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Neurosciences
Robert F. Candia, Leah S. Cohen, Viktoriya Morozova, Christopher Corbo, Alejandra D. Alonso
Summary: This study reveals that Tau can translocate into the nucleus, and the pathological form of Tau exhibits toxicity after nuclear translocation. Hyperphosphorylated Tau not only disrupts microtubule network, but also interferes with cellular functions, ultimately leading to cell death.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shinya Sugimoto, Kunitoshi Yamanaka, Tatsuya Niwa, Yurika Terasawa, Yuki Kinjo, Yoshimitsu Mizunoe, Teru Ogura
Summary: This study demonstrated the role of JDPs in bacterial survival during heat stress and the formation of curli-extracellular amyloid fibers. Genetic analysis revealed the essential role of DnaJ in survival at high temperature and the ability of either DnaJ or CbpA to activate curli production. Biochemical analyses showed that DnaJ and CbpA are involved in regulating the master regulator CsgD and maintaining CsgA in a translocation-competent state.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Shao-Ming Wang, Hsiang-En Wu, Yuko Yasui, Michal Geva, Michael Hayden, Tangui Maurice, Mauro Cozzolino, Tsung-Ping Su
Summary: Autophagy is a crucial cellular process with implications in various diseases. In this study, researchers discovered that the molecular chaperone SIGMAR1 is involved in the transport of TFEB into the nucleus by chaperoning the NP protein POM121, which is responsible for recruiting KPNB1. The disruption of this process in ALS-FTD patients with the C9orf72 subtype leads to impaired autophagy. However, overexpression of SIGMAR1 or POM121, as well as treatment with pridopidine, a SIGMAR1 agonist, can rescue these deficits, suggesting their potential therapeutic use.
Article
Biochemistry & Molecular Biology
Jae Ho Cho, Min Gu Jo, Eun Seon Kim, Na Yoon Lee, Soon Ha Kim, Chang Geon Chung, Jeong Hyang Park, Sung Bae Lee
Summary: Proper regulation of nucleocytoplasmic transport (NCT) is essential for protein function, and dysregulation of NCT is associated with neurodegenerative diseases. In this study, the molecular mechanism of NCT of truncated ATXN3 proteins in comparison with TDP-43 was investigated. The results show that NCT of ATXN3 is regulated by intracellular calcium level and involves Importin alpha 3 (Imp alpha 3), primarily mediated by CBP. Acetyltransferase activity of CBP is important for NCT of ATXN3, which may acetylate Imp alpha 3 to regulate NCT of ATXN3.
MOLECULES AND CELLS
(2022)
Article
Multidisciplinary Sciences
Eugenia M. Clerico, Alexandra K. Pozhidaeva, Rachel M. Jansen, Can Ozden, Joseph M. Tilitsky, Lila M. Gierasch
Summary: This research investigated how Hsp70 binds to full-length client proteins by analyzing complex structures and experiments, revealing that many sequences can choose from multiple binding modes and binding preference depends on the compatibility of substrate residue side chains with binding pockets. The study also found that multiple chaperones can simultaneously bind to multiple sites on the protein substrate, and these sites behave independently.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Matthias P. Mayer
Summary: Hsp70s are evolutionary conserved molecular chaperones that play crucial roles in maintaining protein homeostasis and cell survival. Three mechanistic properties, including binding to degenerate sequence motifs, nucleotide-controlled switching between affinity states, and targeting by cochaperones, make Hsp70s highly versatile.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Genetics & Heredity
Lu Zhao, Ting Jia, Qingsong Jiao, Xueyun Hu
Summary: J-proteins, a type of molecular chaperone, play important roles in chloroplast development, photosynthesis, seed germination, and stress response in plants.
Article
Biology
Shingo Kose, Kenichiro Imai, Ai Watanabe, Akira Nakai, Yutaka Suzuki, Naoko Imamoto
Summary: Hikeshi mediates the nuclear import of HSP70 and regulates its nucleocytoplasmic distribution and functions. Nuclear HSP70 affects the transcriptional activity of HSF1 and nuclear proteostasis. Depletion of Hikeshi leads to reduced nuclear HSP70 levels and up-regulation of HSF1-regulated gene expression.
LIFE SCIENCE ALLIANCE
(2022)
Review
Biochemistry & Molecular Biology
Akanksha Verma, S. Sumi, Mahendra Seervi
Summary: Heat shock proteins (HSPs) play crucial roles in cell survival by interacting with apoptosis signaling components, maintaining protein homeostasis, and regulating the assembly and disassembly of stress granules (SGs) in the cytoplasm, which are important for cell survival under stressful conditions. Their intricate molecular signaling in and around the nexus of HSPs-SGs is essential in managing chronic diseases like cancer and neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Pallabi Mitra, Abhijit S. Deshmukh, Chinmayee Choudhury
Summary: Intracellular pathogens like Toxoplasma gondii manipulate host cell survival and stress response by targeting molecular chaperones such as Heat shock proteins (Hsps), particularly Hsp70. This study demonstrates that the selective inhibitor PES disrupts Hsp70 function, leading to dysregulation of host autophagy and attenuation of T. gondii multiplication. Interestingly, PES targets not only host Hsp70 but also the parasite's Hsp70 homologue, highlighting the potential of dual targeting for therapeutic interventions.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Areeb Jawed, Chi-Ting Ho, Tomas Grousl, Aseem Shrivastava, Thomas Ruppert, Bernd Bukau, Axel Mogk
Summary: To counteract proteotoxic stress and cellular aging, protein quality control (PQC) systems rely on the refolding, degradation and sequestration of misfolded proteins. The Hsp70 chaperone system plays a central role in protein refolding, while degradation is executed by the ubiquitin proteasome system (UPS). In yeast, sequestrase mutants show negative genetic interactions with mutants lacking the Hsp70 co-chaperone Fes1 and the Hsp104 disaggregase and suffering from low Hsp70 capacity. The enhanced activity of the UPS aggravates the growth defects of the mutant cells, while reducing UPS activity improves growth and protein refolding yield.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Anna G. Mankovich, Brian C. Freeman
Summary: Heat shock protein 90 (Hsp90), a highly conserved molecular chaperone, not only maintains the stability of metastable proteins, but also plays a crucial role in protein transport. The specific contributions of Hsp90 to protein transport are still not well defined, despite numerous connections with factors involved in this process.
Article
Oncology
Aifang Wang, Xianmixinuer Abulaiti, Han Zhang, Hang Su, Guangzhi Liu, Shaorong Gao, Lingsong Li
Summary: This study reveals that cancer cells can avoid stress-induced cell death by promoting HSP70-dependent clearance of stress granules. On the other hand, normal cells form persistent stress granules under prolonged stress, leading to cell death. These findings provide insights into tumorigenesis in stressed cells and suggest a new approach to potentially treating cancers by modulating the dynamics of stress granules.
Article
Agronomy
Qiufei Peng, Jieyu Qiu, Xintong Li, Xuezhong Xu, Xinxiang Peng, Guohui Zhu
Summary: Transportation of proteins to and from the nucleus is essential for cellular processes and plant responses to signals and stresses. The nuclear export receptor OsXPO1 was identified in rice and found to play a role in regulating plant development and responses to abiotic stress. Loss-of-function mutations of OsXPO1 resulted in lethal seedlings, while its suppression led to various developmental defects. Overexpression of OsXPO1 reduced plant height but increased tolerance to drought and salt stress in rice.
Article
Cell Biology
Yasuhiro Mimura, Masatoshi Takagi, Michaela Clever, Naoko Imamoto
JOURNAL OF CELL SCIENCE
(2016)
Article
Cell Biology
Khondoker Md Zulfiker Rahman, Hiroshi Mamada, Masatoshi Takagi, Shingo Kose, Naoko Imamoto
Article
Biology
Makoto Kimura, Yuriko Morinaka, Kenichiro Imai, Shingo Kose, Paul Horton, Naoko Imamoto
Review
Cell Biology
Naoko Imamoto
CURRENT OPINION IN CELL BIOLOGY
(2018)
Article
Cell Biology
Yutaka Ogawa, Naoko Imamoto
JOURNAL OF CELL BIOLOGY
(2018)
Article
Biophysics
Changyong Song, Masatoshi Takagi, Jaehyun Park, Rui Xu, Marcus Gallagher-Jones, Naoko Imamoto, Tetsuya Ishikawa
BIOPHYSICAL JOURNAL
(2014)
Article
Biochemistry & Molecular Biology
Yuumi Oda, Makoto Kimura, Shingo Kose, Milo B. Fasken, Anita H. Corbett, Naoko Imamoto
Article
Biochemistry & Molecular Biology
Makoto Kimura, Nobuaki Okumura, Shingo Kose, Toshifumi Takao, Naoko Imamoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Correction
Biochemistry & Molecular Biology
Kimio J. Tanaka, Kenji Ogawa, Masatoshi Takagi, Naoko Imamoto, Ken Matsumoto, Masafumi Tsujimoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Masatoshi Takagi, Yuko Nishiyama, Atsuko Taguchi, Naoko Imamoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Review
Cell Biology
Makoto Kimura, Naoko Imamoto
Article
Biology
Daniel G. Booth, Masatoshi Takagi, Luis Sanchez-Pulido, Elizabeth Petfalski, Giulia Vargiu, Kumiko Samejima, Naoko Imamoto, Chris P. Ponting, David Tollervey, William C. Earnshaw, Paola Vagnarelli
Article
Cell Biology
Takeshi Mizuno, Kei Hirabayashi, Sae Miyazawa, Yurika Kobayashi, Kenta Shoji, Masakazu Kobayashi, Fumio Hanaoka, Naoko Imamoto, Hidetaka Torigoe
Summary: The study investigated the interaction between DNA polymerase alpha and two paralogs of mouse POT1 telomere-binding protein, revealing their roles in lagging strand synthesis. Experimental results showed that the POT1a/b-TPP1-TIN2 complex accumulated in the nucleus, interacted with DNA polymerase alpha, and participated in telomere synthesis.
Article
Biology
Shingo Kose, Kenichiro Imai, Ai Watanabe, Akira Nakai, Yutaka Suzuki, Naoko Imamoto
Summary: Hikeshi mediates the nuclear import of HSP70 and regulates its nucleocytoplasmic distribution and functions. Nuclear HSP70 affects the transcriptional activity of HSF1 and nuclear proteostasis. Depletion of Hikeshi leads to reduced nuclear HSP70 levels and up-regulation of HSF1-regulated gene expression.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)