Article
Pharmacology & Pharmacy
Navodipa Bhattacharya, Nivedita Kolvekar, Sukanta Mondal, Angshuman Sarkar, Dibakar Chakrabarty
Summary: Vipegrin, a 6.8 kDa Kunitz-type serine proteinase inhibitor, has been purified from Russell's viper venom. It inhibits the catalytic activity of trypsin and shows disintegrin-like properties, inhibiting collagen and ADP-induced platelet aggregation. Vipegrin is cytotoxic to MCF7 breast cancer cells and disrupts their adhesion and invasive abilities.
Article
Food Science & Technology
Mimi Lay, Qing Liang, Geoffrey K. Isbister, Wayne C. Hodgson
Summary: Daboia siamensis (Russell's viper) is a venomous snake of medical importance in China and Asia. However, there is little information on the pharmacological properties of the Chinese species' venom and no specific antivenom available in China. This study found that the venom of D. siamensis exhibits neurotoxic and myotoxic effects, which can be prevented by Chinese and Thai antivenoms. Non-specific antivenoms were less effective. A monospecific antivenom for D. siamensis envenomation in China should be further developed.
Article
Food Science & Technology
Khin Than Yee, Jason Macrander, Olga Vasieva, Ponlapat Rojnuckarin
Summary: The venom of Russell's viper in Myanmar has been studied using next-generation sequencing, which has revealed the complexity of venom and its potential applications in understanding snakebite pathogenesis and finding new drugs.
Article
Pharmacology & Pharmacy
D. Pangam, V Jaiswal, P. Dongre
Summary: This study successfully inhibited the activity of Russell's viper venom using chemically synthesized silver nanoparticles, and further analyzed the properties and biocompatibility of the conjugate.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Food Science & Technology
Mimi Lay, Qing Liang, Geoffrey K. Isbister, Wayne C. Hodgson
Summary: In this study, the efficacy of Chinese D. siamensis antivenom alone and in combination with Varespladib was examined for reversing neuromuscular blockade caused by the venom. It was found that the antivenom alone was not able to reverse the neurotoxic and myotoxic effects of the venom at different time points. However, the addition of Varespladib prevented these toxic effects and improved muscle function, indicating its potential as an adjunct treatment. The combination of Varespladib and antivenom did not offer additional benefits compared to Varespladib alone.
Article
Food Science & Technology
Maram Morjen, Wassim Moslah, Imen Touihri-Baraketi, Najet Srairi-Abid, Jose Luis, Naziha Marrakchi, Jed Jebali
Summary: This study successfully cloned and expressed the first active anti-oncogenic snake venom Kunitz-type protease inhibitor, PIVL. It inhibits the growth of glioblastoma cells and blocks angiogenesis. Both native PIVL and recombinant PIVL modulate the PI3/AKT and MAPK signaling pathways.
Article
Medicine, General & Internal
Anjana Silva, Fiona E. Scorgie, Lisa F. Lincz, Kalana Maduwage, Sisira Siribaddana, Geoffrey K. Isbister
Summary: This study compared the recovery of VICC in patients who received and did not receive antivenom following Russell's viper envenoming, showing that antivenom significantly accelerated the recovery of VICC.
FRONTIERS IN MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Christina N. Zdenek, Abhinandan Chowdhury, Grace Y. H. Haw, Aude Violette, Rudy Fourmy, Thomas Christ, Freek J. Vonk, Bryan G. Fry
Summary: The study discovered that the venom of adult and neonate D. russelii snakes had similar toxic effects on human and avian plasma but with varying selectivity for amphibian plasma. Differences in venom potency between adult and neonate snakes may be related to their diet composition.
Article
Infectious Diseases
R. R. Senji Laxme, Suyog Khochare, Saurabh Attarde, Vivek Suranse, Ashwin Iyer, Nicholas R. Casewell, Romulus Whitaker, Gerard Martin, Kartik Sunagar
Summary: In India, snakebite results in a high number of fatalities and morbidities annually, with Russell's viper being a major contributor to these cases. Despite significant intraspecific variation in venom composition and potency across different biogeographic zones in India, commercial antivenoms have shown similar neutralisation potencies against most venom populations, except for low efficacy against the semi-arid population in northern India. There is a pressing need for the development of pan-India effective antivenoms, especially in regions heavily impacted by snakebites.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Pharmacology & Pharmacy
Navodipa Bhattacharya, Nivedita Kolvekar, Sukanta Mondal, Angshuman Sarkar, Dibakar Chakrabarty
Summary: Serine Proteinase Associated Disintegrin-1 (SPAD-1), a novel Snake Venom Serine Proteinase (SVSP), has cytotoxic activity on MCF7 human breast cancer cells. Proteolytic analysis shows it can digest fibrinogen and fibronectin, inhibit collagen and ADP-induced platelet aggregation. It causes visible morphological changes in MCF7 cells and decreases their invasive potency. Confocal microscopic analysis reveals nuclear breakage in SPAD-1-treated cells and it shows disintegrin activity by increasing cell detachment from different culture plate matrices. SPAD-1 may be a potential candidate for future anti-tumor drug design.
Article
Food Science & Technology
Janeyuth Chaisakul, Orawan Khow, Kulachet Wiwatwarayos, Muhamad Rusdi Ahmad Rusmili, Watcharamon Prasert, Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Mongkon Charoenpitakchai, Wayne C. Hodgson, Lawan Chanhome, Narongsak Chaiyabutr
Summary: This study identified two proteins, RvPLA(2) and RvMP, from the venom of Eastern Russell's viper that cause myotoxicity and tissue inflammation in the heart, kidneys, and spleen. These proteins may lead to acute kidney injury and other systemic symptoms. Therefore, there is a need to reevaluate treatment strategies for Eastern Russell's viper envenoming to address potential irreversible nephrotoxicity.
Article
Pharmacology & Pharmacy
Sumudu Priyankara, Vishmi Rathnasiri, Thanigasalan Mihiran, Gayani Premawansa, Geoffrey K. Isbister, Anjana Silva
Summary: Russell's viper envenoming can lead to VICC and AKI, which may be delayed or not detected with clotting tests.
Article
Cardiac & Cardiovascular Systems
Youngeun Lee, Ja-Yoon Gu, Hyun Kyung Kim
Summary: Lupus anticoagulant (LA), composed of heterogeneous autoantibodies, is closely associated with thrombotic events. Patients with positive results for both the dilute Russell viper venom time (dRVVT) and silica clotting time (SCT) were found to have a higher risk of developing thrombotic events, along with higher rates of positivity for aCL and aB2GPI antibodies.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2022)
Article
Pathology
Brenda J. Nelson Strande, Meera Sridharan, Rachel R. Leger, Melissa S. Stuart, Julie Tange, Serena D. Navitska, Nahla Heikal, Aneel A. Ashrani, Dong Chen, Jansen N. Seheult, Rajiv K. Pruthi
Summary: This study investigated the impact of residual platelets in frozen-thawed plasma on the dilute Russell's viper venom time (DRVVT) assay. The results showed that residual platelets in plasma samples resulted in a shortening of DRVVT assay results after one freeze-thaw cycle, which may lead to false-negative LAC test results.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jizhuang Ma, Yongfeng Chen, Keda Zhang, Tianzhi Yang, Huichao Xie, Xinggang Yang, Pingtian Ding
Summary: In this study, researchers developed a novel sclerosing agent called POL-TA. By inducing endothelial cell damage and releasing tranexamic acid, POL-TA can stabilize the fibrin network of thrombus and promote vascular fibrosis, which can be used for the treatment of venous malformations.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)