Article
Cell Biology
Elettra Musolino, Christina Pagiatakis, Federica Pierin, Daniele Sabatino, Giovanna Finzi, Rosalba Gornati, Giovanni Bernardini, Roberto Papait
Summary: Three-dimensional chromatin organization plays a crucial role in defining cell transcription program during development. A new electron-microscopy staining technique called ChromEM has been developed to selectively mark nuclear DNA without altering its structure, allowing better visualization of 3D chromatin conformation. In this study, the protocol for applying ChromEM on myocardial tissue from mice is provided, along with the use of transmission electron microscopy (TEM) to measure morphological parameters of peripheral heterochromatin in cardiomyocytes. This protocol can also be used with electron tomography to study 3D chromatin organization in different aspects of heart pathobiology and establish ChromEM in other tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Ann De Maziere, Jan van der Beek, Suzanne van Dijk, Cecilia de Heus, Fulvio Reggiori, Masato Koike, Judith Klumperman
Summary: This study presents an optimized procedure for localizing endogenous LC3 at ultrastructural resolution. It provides researchers with a tool to study the canonical and non-canonical roles of LC3 in native conditions.
Article
Engineering, Multidisciplinary
Songjie Li, Mengyuan He, Guanjie Hu, Yun Tian, Chengduo Wang, Ben Jing, Dehai Ping
Summary: This study discusses the coarsening process of second-phase particles in a solid-phase matrix, emphasizing the importance of considering the microstructural evolution of the corresponding matrix phase. Experimental observations reveal that the aggregation of fine carbide particles and the shrinkage of cementite layers are key driving forces behind the coarsening of cementite particles and the development of pearlite structure in carbon steels.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Biology
Sebastian Stroeh, Eric W. Hammerschmith, David W. Tank, H. Sebastian Seung, Adrian Andreas Wanner
Summary: The in situ time-lapsed X-ray-assisted staining procedure allows real-time observation of individual staining steps in electron microscopy and measurement of heavy metal accumulation in large tissue samples immersed in different staining solutions. The accumulation of osmium in fixed tissue was found to have a quadratic dependence on incubation time and sample size. This method provides insights for optimizing staining protocols for large samples.
Article
Microscopy
Nimshitha Pavathuparambil Abdul Manaph, Samia M. Ltaief, Wared Nour-Eldine, Janarthanan Ponraj, John Agcaoili, Said Mansour, Abeer R. Al-Shammari
Summary: Transmission electron microscopy (TEM) is a powerful technique for visualizing structural details inside cells. In this study, a detailed step-by-step protocol for preparing blood samples for TEM imaging is provided. This protocol enables robust visualization of the ultrastructures of blood immune cells, and can be used to study ultrastructural changes in these cells under different physiological and disease conditions.
Article
Biochemistry & Molecular Biology
Matteo Pedrazzoli, Manuela Medelin, Federica Marchiotto, Barbara Cisterna, Manuela Malatesta, Mario Buffelli
Summary: Utilizing multiple analysis techniques on a single sample is desirable in research to reduce animal use and maximize information. This study presents a simplified brain tissue staining procedure allowing for multiple analyses in the same sample without requiring special equipment or skills.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Omar Zmerli, Sara Bellali, Gabriel Haddad, Akiko Hisada, Yusuke Ominami, Didier Raoult, Jacques Bou Khalil
Summary: Phosphotungstic acid (PTA) staining is a commonly used negative staining method in scanning electron microscopy (SEM) for assessing the viability of microorganisms. This study demonstrates that PTA staining can effectively differentiate between live and dead bacteria, providing a rapid, cost-effective, and efficient viability assay for microbiome investigations and antimicrobial susceptibility testing.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Joshua B. R. White, Daniel P. Maskell, Andrew Howe, Martin Harrow, Daniel K. Clare, C. Alistair Siebert, Emma L. Hesketh, Rebecca F. Thompson
Summary: Cryo-electron microscopy is a powerful technique for structure determination of macromolecular complexes through single particle analysis, involving vitrification, specimen screening, data collection, and image processing, with options for user modification and common troubleshooting. Variations on imaging protocols to assist users in efficient operation and imaging when physical access to the microscope is limited are being developed due to remote microscope operation becoming standard.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Chemistry, Physical
Thomas Altantzis, Da Wang, Ajinkya Kadu, Alfons van Blaaderen, Sara Bals
Summary: The paper proposes an optimized approach for electron tomography to minimize artifacts related to beam broadening in high angle annular dark-field scanning transmission electron microscopy mode. By acquiring two tomographic tilt series of the same sample and merging the reconstructions, blurring in the reconstructed volume is minimized. This approach, combined with an advanced three-dimensional reconstruction algorithm, can be used to investigate a broad range of samples.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Chemistry, Multidisciplinary
Shiqing Deng, Jun Li, Didrik R. Smabraten, Shoudong Shen, Wenbin Wang, Jun Zhao, Jing Tao, Ulrich Aschauer, Jun Chen, Yimei Zhu, Jing Zhu
Summary: The study reveals the critical roles of individual Sc substitutional atoms in modulating ferroelectricity at the atomic scale of typical multiferroics, Lu1-xScxFeO3, through advanced microscopy and theoretical studies. It shows that atomic variations in polar displacement stabilized by Sc substitution correlate with local chemical and electronic fluctuations, reinforcing FeO5 trimerization magnitude and Lu/Sc-O hybridization strength. This understanding could lead to engineering emergent functionalities of chemically doped functional materials.
Article
Multidisciplinary Sciences
Nobuo Kariyama, Hiromi Sakata-Haga, Tsuyoshi Tsukada, Hiroki Shimada, Makoto Taniguchi, Toshihisa Hatta
Summary: The study developed a rapid bone staining method and explored chemical hair removal treatments for hairy animals. Using these methods enables artifact-free bone staining and provides a new platform for pathological anatomical analysis.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Alexander Muller, Gauthier J-P Deblonde, Peter Ercius, Steven E. Zeltmann, Rebecca J. Abergel, Andrew M. Minor
Summary: In this work, the authors utilize transmission electron microscopy to characterize highly-radioactive compounds of berkelium and californium, requiring only small amounts of the elements. They find that berkelium exhibits unexpectedly weak spin-orbit coupling, while californium follows a jj coupling scheme.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Halima Siddiqui, Nikita Yevstigneyev, Golnoush Madani, Sally McCormick
Summary: Endocytosis is the active transport process of molecules into cells. It plays a crucial role in understanding the regulation of plasma lipid levels and the development of diseases such as atherosclerosis. The pathways of lipoprotein endocytosis have been extensively studied, with a focus on low-density lipoprotein (LDL) and lipoprotein(a) (Lp(a)).
Article
Plant Sciences
Lauren K. Dedow, Emily Oren, Siobhan A. Braybrook
Summary: The beta-glucuronidase gene (uidA/GUS) is a widely used reporter gene in plants. Despite its popularity, there is a lack of specific protocols for minimizing nonrepresentative staining patterns, particularly in challenging tissue types such as dark-grown Arabidopsis hypocotyls. In this study, the researchers developed an optimized staining protocol that combines the use of acetone as a fixative, deliberate physical damage, and proper controls to ensure accurate staining while minimizing false negatives. This protocol can improve the understanding of promoter activity in Arabidopsis hypocotyls and other hard-to-stain tissues.
Article
Food Science & Technology
Eveline Verleysen, Frederic Brassinne, Frederic Van Steen, Nadia Waegeneers, Karlien Cheyns, Ronny Machiels, Stella Mathioudaki, Isaac Ojea Jimenez, Marina Ledecq, Jan Mast
Summary: This study proposed a generic method for determining the size distribution of E171 particles in food, which was evaluated on 20 food products and one personal care product. The analysis showed that some food products contained smaller rutile TiO2 particles coated with mica, while others had a homogeneous distribution of TiO2 particles.
Article
Virology
He Wang, Yongjian Zang, Yizhen Zhao, Dongxiao Hao, Ying Kang, Jianwen Zhang, Zichen Zhang, Lei Zhang, Zhiwei Yang, Shengli Zhang
Summary: The study revealed significant coevolution between hemagglutinin (HA) and neuraminidase (NA) at the sequence level, which is closely related to the type of host species and virus epidemic years. Furthermore, a sequence-to-sequence transformer model (S2STM) was proposed to establish the relationship network between HA and NA sequences.
Review
Pharmacology & Pharmacy
Zhiwei Yang, Zichen Zhang, Yizhen Zhao, Qiushi Ye, Xuhua Li, Lingjie Meng, Jiangang Long, Shengli Zhang, Lei Zhang
Summary: Interactions between organelles are crucial for maintaining normal cell function and homeostasis, and they are also implicated in various diseases and drug discovery. Advanced imaging techniques and fluorescent probes have enabled accurate estimation of subcellular structures, organelle interactions, and drug discovery related to organelles. This study summarizes recent advances in this rapidly evolving field, highlighting the potential of nanoscopy and molecular dynamics simulations in organelle-related drug discovery.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Yimin Zhao, Quan Zhang, Jianbing Ma, Ruobing Yi, Lu Gou, Dexi Nie, Xiaona Han, Lihao Zhang, Yuetian Wang, Xintong Xu, Zhe Wang, Liang Chen, Ying Lu, Shengli Zhang, Lei Zhang
Summary: By integrating high-temperature oxidation and pure-water incubation processes, Cu2O monocrystals with specific crystal planes and good conductivity were successfully synthesized on graphene oxide membranes. The assembly is attributed to the aggregation and attachment of Cu and O in the aqueous environment, as well as the cation-pi interactions between Cu2O and graphene oxide.
Article
Biochemistry & Molecular Biology
Xiaolei Yu, Xin Meng, Yidi Yan, Hui Wang, Lei Zhang
Summary: This study demonstrates that naringin extracted from pomelo peel has the potential to lower blood lipids and protect the liver in hyperlipidemic mice. It shows significant lipid-lowering and hepatoprotective effects, suggesting its potential as a dietary strategy for managing hyperlipidemia.
Article
Chemistry, Multidisciplinary
Yunzhang Li, Yimin Zhao, Xing Liu, Yinwei Qiu, Zhenglin He, Shengli Zhang, Haiping Fang, Liang Chen, Lei Zhang, Guosheng Shi
Summary: This study utilizes cryo-electron microscopy and X-ray photoelectron spectroscopy to discover a novel 2D Li2Cl crystal with metallic properties. A high content of Li2Cl is prepared in reduced graphene oxide (rGO) membranes through vacuum filtration, exhibiting high areal capacitance. Furthermore, the Li2Cl-rGO membranes also exhibit heterostructure property and piezoelectricity, making them useful as humidity sensors. These findings open up possibilities for fabricating novel high-performance electronic devices, transistors, and sensors.
ADVANCED MATERIALS INTERFACES
(2023)
Review
Peripheral Vascular Disease
Han Xue, Meng Zhang, Jianfang Liu, Jianjun Wang, Gang Ren
Summary: This article reviews recent research on the structure, lipid transfer mechanism, and inhibition of cholesteryl ester transfer proteins (CETP). Genetic deficiency in CETP is associated with low levels of LDL-C and high levels of HDL-C, which is correlated with a lower risk of ASCVD. CETP inhibitors have been developed and evaluated in clinical trials, but their poor efficacy against ASCVD has decreased interest in CETP as a therapeutic target.
CURRENT ATHEROSCLEROSIS REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Yizhen Zhao, Yifan Zhao, Linke Xie, Qian Li, Yuze Zhang, Yongjian Zang, Xuhua Li, Lei Zhang, Zhiwei Yang
Summary: The global pandemic of SARS-CoV-2 is a pressing public health issue. This study models the structure and properties of the S protein in different states, identifies potential binding sites for ligands, and screens for bioactive compounds. Molecular dynamic simulations reveal that sulforaphane has the best binding affinity and can inhibit the conformational changes of the S protein during viral membrane fusion. These findings contribute to the understanding of S protein regulation and structure-activity relationship, facilitating the development of potential antiviral agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xiao-Lei Yu, Xin Meng, Yi-Di Yan, Jin-Cheng Han, Jia-Shan Li, Hui Wang, Lei Zhang
Summary: The naringin extraction process was optimized using response surface methodology and the purified naringin showed potential applications as a functional food for lowering blood lipid levels.
Article
Nanoscience & Nanotechnology
Ewan K. S. McRae, Helena Ostergaard Rasmussen, Jianfang Liu, Andreas Boggild, Michael T. A. Nguyen, Nestor Sampedro Vallina, Thomas Boesen, Jan Skov Pedersen, Gang Ren, Cody Geary, Ebbe Sloth Andersen
Summary: RNA origami is a method for designing RNA nanostructures that can self-assemble through co-transcriptional folding. This study uses cryogenic electron microscopy to determine the structure of RNA origami sheets and bundles, revealing structural parameters and folding principles. The study also combines sheets and bundles to construct a multidomain satellite shape and characterizes its flexibility using cryo-electron tomography.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xinming Xia, Yingying Huang, Bingquan Peng, Tao Wang, Ruobing Yi, Yimin Zhao, Jie Jiang, Fangfang Dai, Yan Fan, Pei Li, Yusong Tu, Lei Zhang, Haiping Fang, Liang Chen
Summary: A high-yield synthesis method for 2D abnormal crystals with unconventional stoichiometries is reported, and the unique electronic, magnetic, and optical properties of these crystals are demonstrated. By applying a negative potential, a more than tenfold increase in abnormal Na2Cl crystals with higher sodium content is achieved in rGOM. The crystals exhibit a square structure and piezoelectric behavior.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yizhen Zhao, Dongxiao Hao, Yifan Zhao, Shengli Zhang, Lei Zhang, Zhiwei Yang
Summary: This study compared the structural characteristics of authentic CETP and mutant CETP through molecular dynamics simulations, revealing that authentic CETP exhibits greater flexibility and curvature in lipid transport. A ligand identified through virtual screening and simulations was found to bind to the N- and C-termini of CETP, inducing structural changes unfavorable to lipid transport. This study sheds new light on the relationship between the structural and functional mechanisms of CETP and provides novel ideas for precise regulation of CETP functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yidong Xia, Jianfang Liu, Rahul Kancharla, Jiaoyan Li, Seyed M. Hatamlee, Gang Ren, Viktoriya Semeykina, Ahmed Hamed, Joshua J. Kane
Summary: To study the flow and transport properties of fluids for environmental research, such as geological subsurface energy storage or carbon capture, the detailed 3D morphology of monodispersed synthetic mesoporous silica nanoparticles (MSNs) with diameters below 50 nm was obtained using Individual Particle cryogenic transmission Electron Tomography (IPET) technique. The 3D reconstructed density-maps showed that the diameters of these MSNs varied from 35-46 nm, containing connected intraparticle pores with diameters of 2-20 nm and a mean diameter of 9.2 +/- 3 nm, which is comparable to the mean interparticle pore diameters in sintered agglomerate. The characterization of the pore shape and dimensions provides important information for estimating the flow and transport properties of fluids within the sintered agglomerate of MSNs and for modeling the atomic MSN structures needed for pore-fluid simulations.
NANOSCALE ADVANCES
(2023)
Article
Chemistry, Physical
Zhiwei Yang, Yongjian Zang, He Wang, Ying Kang, Jianwen Zhang, Xuhua Li, Lei Zhang, Shengli Zhang
Summary: CD147 functions as the receptor of extracellular cyclophilin A (CypA) and plays a crucial role in the development of various viral infections. However, the mechanism by which CD147 cooperates with CypA in the cytokine storm is largely unknown, and the binding profile of CD147 with CypA needs further elucidation. This study provides insights into the binding process between CD147 and CypA, highlighting the potential role of P180-G181 in modulating their interaction and the importance of residue R201 in CD147-CypA binding.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Azhar Saeed, Marco Poppe, Matthias B. Wagner, Sebastian Hauche, Christian Anders, Yu Cao, Lei Zhang, Carsten Tschierske, Feng Liu
Summary: Rod-like liquid crystalline polyphilic compounds with a linear oligo(phenyleneethynylene) core, sticky glycerol groups at each end, and long alkyl side chains can form rhombic honeycomb structures with inner angles of 60/120 degrees.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Yongjian Zang, He Wang, Ying Kang, Jianwen Zhang, Xuhua Li, Lei Zhang, Zhiwei Yang, Shengli Zhang
Summary: In this study, the conformational transformations of p38 alpha induced by TAB1 binding were investigated using molecular dynamics simulations and principal component analysis. The results revealed the movement and extension of the activation loop, as well as the formation of a stronger hydrogen bond network. Additionally, an intermediate state with an extended and phosphorylated activation loop was explored.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)