Review
Chemistry, Inorganic & Nuclear
Andre M. N. Silva, Tania Moniz, Baltazar de Castro, Maria Rangel
Summary: Transferrin (Tf) is an essential protein found in all metazoans, responsible for binding and transporting ferric ions, increasing Fe(III) solubility and preventing its pro-oxidant role. In humans, Tf safely transports iron through circulation and delivers it to cells in need. Understanding the factors governing Tf's expression, catabolism, and modifications is crucial for comprehending its role in human pathology and therapeutic potential.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Biochemistry & Molecular Biology
Suliman Khan, William C. Cho, Arif Hussain, Sadaf Azimi, Mohammad Mahdi Nejadi Babadaei, Samir Haj Bloukh, Zehra Edis, Mesha Saeed, Timo L. M. ten Hagen, Hosein Ahmadi, Mahsa Ale-Ebrahim, Laila Abdulmohsen Jaragh-Alhadad, Rizwan Hasan Khan, Mojtaba Falahati, Xiaoju Zhang, Qian Bai
Summary: In biological systems, nanoparticles (NPs) have complex interactions with proteins, including the formation of protein corona and structural changes. These interactions depend on both the physicochemical properties of NPs and the stability of protein molecules. This review discusses the direct bio-chemical and biophysical interactions between NPs and blood proteins, specifically transferrin. The study explores the effects of NP properties on protein structure, transferrin stability, and its applications in drug delivery systems for cancer therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Inorganic & Nuclear
Prerna Yadav, Olivier Blacque, Andreas Roodt, Felix Zelder
Summary: The Fe-salen based probe showed promising results in detecting pyrophosphate through detailed UV-Vis and fluorescence mechanistic studies. The molecular subunits disassemble in the presence of pyrophosphate analyte, with the aqua form of the complex (1-OH2) identified as the active species. The flexibility in molecular recognition and binding of pyrophosphate by the probe explains its outstanding selectivity over other (poly)oxophosphates and potentially competing analytes.
INORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Nutrition & Dietetics
Mizuki Takaragawa, Keishoku Sakuraba, Yoshio Suzuki
Summary: Women are prone to iron deficiency due to increased iron excretion during menstruation. Oral iron supplementation is commonly used to treat this, but it can have side effects and low absorption. Previous research found that consuming foods containing Lactococcus lactis subsp. cremoris H61 (H61) increased serum iron levels, but the causal relationship was unclear. This study aimed to investigate the effects of H61 on the iron status of young women. Results showed that ingesting heat-killed H61 significantly elevated serum iron, transferrin saturation, and ferritin levels, suggesting that H61 may enhance iron absorption and improve iron status.
Article
Multidisciplinary Sciences
Hannah Ochner, Sven Szilagyi, Moritz Edte, Tim K. Esser, Stephan Rauschenbach, Luigi Malavolti, Klaus Kern
Summary: Low-energy electron holography (LEEH) can explore the conformational space of proteins and distinguish different conformations of transferrin, indicating its viability for imaging function-related conformational changes in individual proteins.
SCIENTIFIC REPORTS
(2023)
Article
Neurosciences
Veronica T. Cheli, Diara A. Santiago Gonzalez, Rensheng Wan, Shaina L. Rosenblum, Giancarlo E. Denaroso, Christina G. Angeliu, Zachary Smith, Congying Wang, Pablo M. Paez
Summary: By disrupting the expression of the Tf receptor gene in oligodendrocyte progenitor cells, researchers found that the Tf cycle is uniquely important for the development and function of oligodendrocytes. Deleting the Tfr gene resulted in impaired iron absorption and hypomyelination in mice. The Tf cycle plays a crucial role in iron homeostasis and energy production in oligodendrocyte progenitor cells.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jacob J. Weber, Maithri M. Kashipathy, Kevin P. Battaile, Eden Go, Heather Desaire, Michael R. Kanost, Scott Lovell, Maureen J. Gorman
Summary: Transferrins play a crucial role in iron sequestration and transport by tightly binding iron ions. The crystal structure of insect transferrin-1 (Tsf1) revealed a unique iron coordination mechanism different from vertebrate transferrins. The presence of carbonate anions in insect transferrin suggests its potential to coordinate alternative ions.
Article
Biochemistry & Molecular Biology
Valentina Borko, Tomislav Friganovic, Tin Weitner
Summary: The thermodynamic parameters of ferric ions binding to human serum transferrin (hTf) were determined in the presence of carbonate and oxalate. The results indicate that the binding of ferric ions to hTf is driven by both enthalpy and entropy, with different contributions from different binding sites. Lower sialic acid content in hTf leads to stronger binding enthalpies, while the presence of carbonate increases the apparent binding constants. Sialylation also affects the heat change rates for binding sites, but only in the presence of carbonate.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Aswin Kottapurath Vijay, Virender K. Sharma, Dan Meyerstein
Summary: Iron(II), Fe-II, plays a crucial role in various natural and biological reactions. Understanding the mechanism and rates of Fe-II oxidation by molecular oxygen (O-2) in the presence of bicarbonate (HCO3-) is essential due to its high concentration in environmental conditions. While previous studies suggested the formation of reactive oxygen species, this study demonstrates the rapid generation of carbonate radicals (CO3 center dot-) in Fe-II oxidation by O-2 in the presence of bicarbonate. The findings highlight the importance of considering CO3 center dot- formation in the geochemical cycling of iron and carbon.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Kristyn Hayashi, Kenton L. Longenecker, Yi-Liang Liu, Bryan Faust, Aditi Prashar, Johannes Hampl, Vincent Stoll, Sandro Vivona
Summary: Melanotransferrin has been found to have multiple functions, acting as both a mediator of iron uptake and cell proliferation on cells, and a transporter of iron across the blood-brain barrier in serum. Its specific structure also provides a reference point for drug design.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Fraser J. Graham, Pierpaolo Pellicori, Gabriele Masini, Joseph J. Cuthbert, Andrew L. Clark, John G. F. Cleland
Summary: This study explored the determinants of transferrin saturation (TSAT) in patients with heart failure, and the influence of serum transferrin concentration (STC) on TSAT and mortality. The results showed that lower STC (≤2.3 g/L) was associated with older age, lower iron concentration, lower hemoglobin, and higher inflammation markers. After adjusting for age, cardiac markers, and hemoglobin, both higher iron concentration and STC were negatively associated with mortality. These patients often have anemia and poor prognosis, but they are currently excluded from clinical trials of iron repletion.
Article
Nutrition & Dietetics
Seok-Hui Kang, Bo-Yeon Kim, Eun-Jung Son, Gui-Ok Kim, Jun-Young Do
Summary: The aim of this study was to evaluate the survival rates of patients on maintenance hemodialysis (HD) according to their iron status. The study analyzed data from the National HD Quality Assessment Program and claims data (n = 42,390). The patients were divided into four groups based on their transferrin saturation rate and serum ferritin levels. The results showed that patients with normal iron status had the highest survival rates, while there were minimal differences in survival rates among the groups with abnormal iron status. Subgroup analyses based on age, hemoglobin, or serum albumin levels showed different trends.
Article
Biochemistry & Molecular Biology
Ewa Skarzynska, Monika Wrobel, Hanna Zborowska, Mateusz Franciszek Kolek, Grzegorz Manka, Mariusz Kiecka, Michal Lipa, Damian Warzecha, Robert Spaczynski, Piotr Piekarski, Beata Banaszewska, Artur Jakimiuk, Tadeusz Issat, Wojciech Rokita, Jakub Mlodawski, Maria Szubert, Piotr Sieroszewski, Grzegorz Raba, Kamil Szczupak, Tomasz Kluz, Marek Kluza, Miroslaw Wielgos, Barbara Lisowska-Myjak, Piotr Laudanski
Summary: The aim of this study was to investigate the relationship between lactoferrin and iron and its binding proteins in women with endometriosis. The results showed differences in lactoferrin, transferrin, ferritin, and iron levels between plasma and peritoneal fluid. Lactoferrin levels in plasma were associated with iron and transferrin in endometriosis, while lactoferrin in peritoneal fluid correlated with lactoferrin, iron, and transferrin in patients without endometriosis. The study suggests that assessing iron metabolism may be useful in diagnosing and monitoring endometriosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Kylie Yang, Jessica E. Boles, Lisa J. White, Kira L. F. Hilton, Hin Yuk Lai, Yifan Long, Jennifer R. Hiscock, Cally J. E. Haynes
Summary: In this study, we report a water-soluble, supramolecular self-associating amphiphile that acts as a cation uniporter in synthetic vesicle systems. Mechanistic insights were obtained through planar bilayer patch clamp experiments.
Article
Chemistry, Multidisciplinary
Jingjing Li, Ying Zhang, Jianghui Sun, Jin Ouyang, Na Na
Summary: The study presented a highly efficient platform for nucleic acid framework materials, achieving chemotactic recognition of cancer cells, programmable and visualized drug delivery, and synergistic therapy, maximizing the therapeutic efficacy.
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)