Article
Biochemistry & Molecular Biology
Genta Kamiya, Nobuo Kitada, Tadaomi Furuta, Takashi Hirano, Shojiro Maki, Sung Bae Kim
Summary: The present study introduces a unique BL signature imaging system with novel CTZ analogues named C-series. It examines the BL intensity patterns and spectra of nine C-series CTZ analogues according to marine luciferases. The results show that four CTZ analogues, C3, C4, C6, and C7, luminesce with distinctive BL spectral signatures and intensity patterns according to different luciferases. These findings provide a unique methodology to specify each signal in a single- or multi-reporter system using unique BL signatures and patterns of reporter luciferases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Gaku Mizuno, Daichi Yano, Jose Paitio, Hiromitsu Endo, Yuichi Oba
Summary: The lantern shark genus Etmopterus consists of approximately 40 species of bioluminescent cartilaginous fishes that emit blue light mainly from their ventral body surface. This study detected both coelenterazine and coelenterazine-dependent luciferase activity in the ventral photophore tissue of Etmopterus molleri, suggesting that bioluminescence in lantern sharks is produced using coelenterazine as a substrate for the luciferin-luciferase reaction, similar to some luminous bony fishes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Aisha J. Syed, James C. Anderson
Summary: Bioluminescence is a natural phenomenon where living creatures produce light through a reaction between luciferin and luciferase. Various luciferin-luciferase pairs have different applications, such as gene assays and drug discovery. In recent years, bioluminescence has found increasing applications in biomedicine, including photodynamic therapy and neuron control.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Biology
Svetlana V. Markova, Marina D. Larionova, Igor A. Korotov, Eugene S. Vysotski
Summary: We have identified the minimal sequence required for the bioluminescent activity of copepod luciferases from Metridia longa and Gaussia princeps. This sequence displays high homology with other copepod luciferases, suggesting that the boundaries of the catalytic domain are the same for all known copepod luciferases. We have also demonstrated the involvement of the flexible C-terminus in retaining the bioluminescent reaction product and the successful use of a shortened mutant as a miniature bioluminescent reporter in living cells.
Article
Biochemistry & Molecular Biology
Shusei Kanie, Mami Komatsu, Yasuo Mitani
Summary: Lucensosergia lucens lacks known or homologous coelenterazine-dependent luciferase genes, but RNA-Seq analysis suggests the presence of unknown proteinaceous components for coelenterazine luminescence.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Sung-Bae Kim, Tadaomi Furuta, Nobuo Kitada, Shojiro A. A. Maki
Summary: A series of new artificial luciferases (ALucs) were created by compensating missing peptide blocks in the alignment of existing ALuc sequences. These new ALucs formed an independent branch genetically isolated from other natural marine luciferases and showed bioluminescent properties in living mammalian cells. The success of this study can provide new insights into engineering and functionalizing marine luciferases for various applications in bioassays and molecular imaging.
Article
Biochemistry & Molecular Biology
Daichi Yano, Manabu Bessho-Uehara, Jose Paitio, Masakazu Iwasaka, Yuichi Oba
Summary: Research suggests that the luciferase of lanternfish may consist of 14-3-3 proteins, which share similar molecular weights and properties. The addition of specific compounds could potentially inhibit the luciferase activity.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2022)
Article
Chemistry, Analytical
Elham Karimi, Maryam Nikkhah, Saman Hosseinkhani
Summary: Using gold nanoparticles as an enzyme immobilizer can increase the activity of luciferases and improve signal stability in the presence of nanoparticles. The application of ATP in the firefly luciferase reaction increases luciferase activity and enhances detection limits.
Article
Biochemistry & Molecular Biology
Genta Kamiya, Nobuo Kitada, Tadaomi Furuta, Takashi Hirano, Shojiro A. Maki, Sung-Bae Kim
Summary: A unique combinatorial bioluminescence imaging system was developed to determine molecular events in mammalian cells. This system utilizes various colors and BL intensity patterns by using reporter luciferases and novel coelenterazine analogues. The synthesized substrates showed unique specificity and BL intensity patterns with marine luciferases, enabling the identification of different reporters and ligand-specific fold intensities in mammalian cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Ziyuan Liu, Zhongyong Qiu, Chunju He
Summary: Inspired by Watasenia Scintillans, a novel antifouling coating with highly hydrophilic surface and luminescence layer is reported. The coating shows excellent performance in resisting non-specific protein adhesion and attachment of fouling organisms. The long-term underwater field tests also demonstrate its durability and effectiveness in inhibiting various biological fouling.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Hector Martinez-Perez-Cejuela, Maria Maddalena Calabretta, Valerio Bocci, Marcello D'Elia, Elisa Michelini
Summary: ATP determination is commonly used for microbial contamination assessment in different settings. However, commercially available kits require controlled temperature storage and transportation, limiting their applicability. To address this issue, a new bioluminescence paper biosensor was developed by combining a new luciferase mutant with a metal-organic framework (MOF). This sensor showed improved bioluminescence signal and stability, and successfully analyzed ATP content in complex biological matrices with low detection limits. The developed sensor has potential applications in various fields requiring ATP monitoring.
Article
Chemistry, Analytical
Maria Maddalena Calabretta, Denise Gregucci, Hector Martinez-Perez-Cejuela, Elisa Michelini
Summary: The new mutant luciferase BgLuc has desirable properties for bioanalytical applications, improving the performance of methods such as ATP detection, whole-cell biosensors, and viability assays.
Article
Biochemistry & Molecular Biology
Sung-Bae Kim, Ryo Nishihara, Ramasamy Paulmurugan
Summary: We identified the best resonance energy donor for near infrared bioluminescence resonance energy transfer (NIR-BRET) templates and achieved high sensitivity in monitoring molecular events in cells. The established templates, fused with fluorescent proteins, showed a large blue-to-red shift and were used in new NIR-BRET systems for imaging steroid hormone activities. The NIR-BRET systems demonstrated specific luminescence signals upon exposure to different steroid hormones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Joao Sousa, Carla M. Magalhaes, Patricia Gonzalez-Berdullas, Joaquim C. G. Esteves da Silva, Luis Pinto da Silva
Summary: Chemi- and bioluminescence are remarkable light-emitting phenomena that convert thermal energy into excitation energy through (bio)chemical reactions. In this study, a novel dibrominated Coelenterazine analog with superior chemiluminescent properties was synthesized and characterized. This compound exhibited enhanced luminescence in terms of total light output and emission lifetime compared to natural imidazopyrazinones and commercially available analogs in aprotic media, showing potential as a basis for optimized probes for reactive oxygen species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Marina D. Larionova, Lijie Wu, Elena Eremeeva, Pavel Natashin, Dmitry Gulnov, Elena Nemtseva, Dongsheng Liu, Zhi-Jie Liu, Eugene S. Vysotski
Summary: Coelenterazine-v (CTZ-v) is a suitable substrate for deep tissue imaging with Renilla luciferase, but Ca2+-regulated photoproteins activated by CTZ-v have low bioluminescence activity and are unsuitable for calcium imaging. The low bioluminescence activity of obelin-v is attributed to the low efficiency of generating an electronic excited state, possibly due to small changes in the substrate microenvironment at the active site.
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)