Article
Microbiology
Er-Ying Jiang, Yong Fan, Nghi-Van Phung, Wan-Yue Xia, Guang-Rong Hu, Fu-Li Li
Summary: Plastoglobules are lipoprotein structures in plant chromoplasts and chloroplasts, involved in carotenoid metabolism and storage. In this study, the major protein in plastoglobules, PAP, was overexpressed in Phaeodactylum tricornutum. Mutants showed decreased plastoglobule diameter and increased fucoxanthin level. They also exhibited morphological differences and increased unsaturated fatty acid levels. Transcriptomic analysis revealed altered xanthophyll synthesis and storage, affecting the assembly of fucoxanthin chlorophyll a/c-binding protein and reducing non-photochemical quenching activity. Modulating PAP expression can achieve metabolic regulation at the suborganelle level.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yu Bai, Tianjun Cao, Oliver Dautermann, Paul Buschbeck, Michael B. Cantrell, Yinjuan Chen, Christopher D. Lein, Xiaohuo Shi, Maxwell A. Ware, Fenghua Yang, Huan Zhang, Lihan Zhang, Graham Peers, Xiaobo Li, Martin Lohr
Summary: Fucoxanthin is a major light-harvesting pigment in ecologically important algae. The biosynthetic pathway of fucoxanthin has been identified by studying knockout mutants of the diatom Phaeodactylum tricornutum. The pathway is more complex than expected and involves the metabolism of diadinoxanthin as a central regulatory hub. The study also reveals the evolution of genes for xanthophyll cycle enzymes and the alternative pathway in brown algae.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
To Quyen Truong, Yun Ji Park, Song Yi Koo, Jae-Hyeong Choi, Altai Enkhbayar, Dae-Geun Song, Sang Min Kim
Summary: In this study, it was found that low light intensity conditions led to higher FX content and productivity. The crucial roles of PtLhcf5 and PtLhcf8 in FCP biosynthesis under low light intensity were revealed. FCP formation only occurred in the early growth stage under light transition, and FCP degradation could be triggered by high light intensity throughout the cultivation period. Therefore, low light intensity conditions in the early growth stage were critical for higher FX productivity.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Cell Biology
Jared J. Stewart, William W. Adams, Marina Lopez-Pozo, Naiara Doherty Garcia, Maureen McNamara, Christine M. Escobar, Barbara Demmig-Adams
Summary: The study demonstrates that duckweed has unique functional characteristics, being able to adapt to different light conditions and exhibiting a combination of traits of fast-growing annuals and slow-growing evergreens.
Article
Biochemistry & Molecular Biology
Reimund Goss, Daniela Volke, Lina Emilia Werner, Ronja Kunz, Marcel Kansy, Ralf Hoffmann, Christian Wilhelm
Summary: In this study, fucoxanthin chlorophyll protein (FCP) complexes of the centric diatom Thalassiosira pseudonana were prepared using low concentrations of a mild detergent and sucrose gradient ultracentrifugation. Two main FCP fractions were observed in the sucrose gradients, each with different protein, lipid, and pigment composition. Mass spectrometry analysis revealed the presence of the xanthophyll cycle enzyme DDE in both FCP fractions, with higher enrichment in the fraction enriched in FCP-A. In-vitro de-epoxidation assays showed a more pronounced conversion of DD to Dt in the fraction enriched in FCP-A compared to FCP-B.
Article
Biochemistry & Molecular Biology
Setareh Jamali Jaghdani, Peter Jahns, Merle Traenkner
Summary: This study investigated the impact of varying magnesium concentrations on barley photosynthetic efficiency and photoprotective responses, revealing that CO2 assimilation is more sensitive to reduction of tissue magnesium concentrations, while supply with lower magnesium concentrations induced photo oxidative stress.
Article
Agricultural Engineering
Yuelian Li, Han Sun, Yinan Wang, Shufang Yang, Jia Wang, Tao Wu, Xue Lu, Yawen Chu, Feng Chen
Summary: This study investigated the effects of regulation of photosystem and central carbon metabolism on cell growth and fucoxanthin accumulation in Isochrysis zhangjiangensis. The results showed that high light conditions promoted biomass accumulation but decreased fucoxanthin productivity. High light suppressed active photosystem and reduced chlorophyll content, but improved metabolic flux for massive biomass accumulation. Furthermore, high light inhibited genes involved in fucoxanthin biosynthesis and induced a diadinoxanthin cycle. Constant light at 100 μmol m(-2) s(-1) provided the highest fucoxanthin productivity.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Uthman O. Badmus, Alexander Ac, Karel Klem, Otmar Urban, Marcel A. K. Jansen
Summary: UV exposure induces changes in carotenoid biosynthesis and accumulation in plants, but there is no consensus on the direction and amplitude of these effects. Meta-analysis of published studies revealed that only violaxanthin was consistently induced by UV exposure. Changes in violaxanthin accumulation were accompanied by decreases in antheraxanthin and zeaxanthin.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Petra D'Odorico, Leonie Schoenbeck, Valentina Vitali, Katrin Meusburger, Marcus Schaub, Christian Ginzler, Roman Zweifel, Vera Marjorie Elauria Velasco, Jonas Gisler, Arthur Gessler, Ingo Ensminger
Summary: Monitoring tree physiological responses to drought using drone-based remote sensing and PRI revealed that non-irrigated trees showed higher stress levels compared to irrigated trees. Long-term acclimation influenced the seasonal relationship between PRI and soil water availability. This study demonstrates the importance of remote sensing techniques in scaling tree responses to drought stress and the persistence of legacy effects even after irrigation cessation.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Nutrition & Dietetics
Mi-Bo Kim, Hyunju Kang, Yang Li, Young-Ki Park, Ji-Young Lee
Summary: The study demonstrated that FCX exerts anti-inflammatory and antioxidant effects in macrophages through activation of NRF2 nuclear translocation, at least partly mediated by the PI3K/AKT pathway.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Review
Plant Sciences
Andrew J. Simkin, Leepica Kapoor, C. George Priya Doss, Tanja A. Hofmann, Tracy Lawson, Siva Ramamoorthy
Summary: Photosynthetic pigments, including chlorophyll, carotenoids, and phycobilins, are essential for efficient light absorption and adaptation to different environments in photosynthetic organisms. They play crucial roles in light harvesting, photoprotection, and deep water colonization.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Plant Sciences
Lulu Ren, Hongping Ma, Donghui Chao, Haizhen Zhang, Kun Qiao, Shuang Feng, Aimin Zhou
Summary: In this study, the functions of two Arabidopsis (Arabidopsis thaliana) Sep proteins, Sep1 and Sep2, in Chl breakdown and in response to light stress were investigated and compared. It was found that Sep2 reduced the levels of Chl by promoting Chl breakdown and could alleviate the accumulation of reactive oxygen species under long-term light stress. However, Sep1 did not cause Chl breakdown in Arabidopsis leaves. These findings suggest that Sep2, as a member of the LHC-like family, might regulate the levels of free Chl by binding to Chl and participate in light-stress response.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Beatriz Fernandez-Marin, Thomas Roach, Amy Verhoeven, Jose Ignacio Garcia-Plazaola
Summary: Xanthophyll cycles play a crucial role in photoprotection and are not limited to excess light conditions but also occur under various abiotic stress conditions, including darkness. The dark-induced formation of zeaxanthin enhances stress tolerance in photosynthetic eukaryotes, yet more research is needed to fully understand the mechanisms and physiological roles of this process.
Article
Spectroscopy
P. Vitek, K. B. Mishra, A. Mishra, B. Vesela, H. Findurova, K. Svobodova, M. Oravec, P. P. Sahu, K. Klem
Summary: Exploring photosynthetic processes via non-invasive means provides additional advantages in phenotyping and precision agriculture. This study used Raman spectroscopy and fluorescence-based methods to investigate the changes in photosynthetic processes and protective mechanisms in Arabidopsis thaliana. The results revealed oxidative damage and depletion of carotenoids under high light conditions, as well as increased accumulation of phenolics. The mutant ros1 showed lower accumulation of anthocyanin compared to the wild-type, supporting the findings from Raman spectroscopy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biodiversity Conservation
Rosina Magana Ugarte, Maria Pilar Gomez-Serranillos, Adrian Escudero, Rosario G. Gavilan
Summary: Despite the high levels of UVB radiation, high temperatures, and drought stress in Mediterranean high-mountain plants, efficient photoprotective strategies are understudied. Different plant species exhibit significant differences in the functioning of the xanthophyll cycle, with some using it as a continuous photoprotective strategy and others using it only under adverse conditions. The involvement of the lutein cycle is considered crucial for sustaining photoprotection in these plants, enhancing their photosystems' optimal functioning.
Review
Plant Sciences
Alexandrina Stirbet, Dusan Lazar, Ya Guo, Govindjee Govindjee
Article
Plant Sciences
M. Bednarikova, Y. Folgar-Camean, Z. Kucerova, D. Lazar, M. Spundova, J. Hajek, M. Bartak
Article
Plant Sciences
David Fuente, Dusan Lazar, Jose Vicente Oliver-Villanueva, Javier F. Urchueguia
Summary: The study reconstructed the absorption spectrum of different strains by summing the computed signature of all pigments present in the organism. Modifications to in vitro pigment spectra were made and a plausible shape for the in vivo absorption spectrum of each chromophore was outlined. The study also provided estimates of pigment concentrations without relying on spectrophotometric correlations, and accurately reproduced experimental spectra for studied wavelengths in various strains.
PHOTOSYNTHESIS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Harry W. Rathbone, Katharine A. Michie, Michael J. Landsberg, Beverley R. Green, Paul M. G. Curmi
Summary: Cryptophyte algae developed a unique antenna structure through evolution from a complex between a red algal scaffolding protein and phycoerythrin beta. The interaction of these scaffolding proteins with phycoerythrin beta led to the demise of the red algal phycobilisome and the emergence of the cryptophyte antenna. Through analysis of phycobilisome cryo-EM structures, a new family of scaffolding proteins related to red algal phycobilisome linker proteins was identified, shedding light on the origin of the cryptophyte antenna.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Loay J. Jabre, Andrew E. Allen, J. Scott P. McCain, John P. McCrow, Nancy Tenenbaum, Jenna L. Spackeen, Rachel E. Sipler, Beverley R. Green, Deborah A. Bronk, David A. Hutchins, Erin M. Bertrand
Summary: The study found that increased iron availability and warming in the Southern Ocean can significantly enhance nutrient uptake and primary productivity in surface ocean microbial communities, with a greater effect observed under simultaneous iron addition and warming. Furthermore, the dominant diatom Pseudo-nitzschia showed increased abundance under warming conditions, attributed to its up-regulation of iron-conserving photosynthetic processes and improved iron uptake and storage mechanisms. These findings highlight important physiological differences between diatom groups and suggest potential shifts in phytoplankton assemblages and nutrient cycling in iron-limited Southern Ocean ecosystems under warming conditions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Plant Sciences
D. Lazar, A. Stirbet, L. O. Bjorn, G. Govindjee
Summary: Oxygenic photosynthesis occurs in the thylakoid membranes, where light is absorbed by pigments and transferred to the photosynthetic reaction centers. The color and intensity of light exposure affect the composition and structure of light-harvesting complexes, thus influencing the photosynthetic process and the organism as a whole.
Correction
Plant Sciences
Iva Ilikova, Petr Ilik, Monika Opatikova, Rameez Arshad, Lukas Nosek, Valav Karlicky, Zuzana Kucerova, Pavel Roudnicky, Pavel Pospisil, Dusan Lazar, Jan Bartos, Roman Kouril
Article
Plant Sciences
Anna A. Hippmann, Nina Schuback, Kyung-Mee Moon, John P. McCrow, Andrew E. Allen, Leonard F. Foster, Beverley R. Green, Maria T. Maldonado
Summary: Diatoms are a successful group of phytoplankton in the oceans, contributing to a significant part of the Earth's photosynthetic productivity. Their genomes include genes from various sources, resulting in differential regulation of metabolic pathways under nutrient limitation. This study focused on the metabolic changes in response to copper (Cu) limitation in diatoms, revealing integrated responses involving nitrogen assimilation, photosynthesis, and oxidative stress resistance.
Article
Plant Sciences
Hui Lyu, Dusan Lazar
Summary: The transthylakoid membrane potential plays a crucial role in driving ATP synthesis and regulating photosynthesis under environmental stresses, with cation binding influencing the electric potentials adjustment. However, a comprehensive understanding of the impact of altered cationic binding on the membrane surface is still lacking.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dusan Lazar, Yuxi Niu, Ladislav Nedbal
Summary: This study exposed pea leaves to oscillating light and found that the dynamics of photosynthesis depend on the oscillation period, revealing information about the underlying regulatory networks. The regulation aims to keep the reaction centers of PSI and PSII open. Further experiments, mathematical modeling, and system identification and control tools are expected to enhance our understanding of these regulatory networks.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Hui Lyu, Dusan Lazar
Summary: The light-induced transthylakoid membrane potential (Delta psi) plays a crucial role in ATP synthesis and in the acclimation of photosynthesis to changing light conditions. However, the mechanisms underlying ion fluxes and their impact on Delta psi regulation remain unclear.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Yuxi Niu, Dusan Lazar, Alfred R. Holzwarth, David M. Kramer, Shizue Matsubara, Fabio Fiorani, Hendrik Poorter, Silvia D. Schrey, Ladislav Nedbal
Summary: The study focused on the rapid regulation of photoprotective mechanisms in response to changing light in plants. It was found that PsbS regulation had the fastest response time to oscillating light with periods longer than 10 seconds. Processes involving violaxanthin de-epoxidase dampened changes in chlorophyll fluorescence in oscillation periods of 2 minutes or longer. Knocking out the PGR5/PGRL1 pathway strongly reduced variations in all monitored parameters, possibly due to congestion in the electron transport. The observed dynamics of photosystem I components were interpreted as being formed by thylakoid remodeling in slow light oscillations.
Article
Plant Sciences
Iva Ilikova, Petr Ilik, Monika Opatikova, Rameez Arshad, Lukas Nosek, Vaclav Karlicky, Zuzana Kucerova, Pavel Roudnicky, Pavel Pospisil, Dusan Lazar, Jan Bartos, Roman Kouril
Summary: The study examined the structure-function relationship of PSII supercomplexes in Arabidopsis mutants to explore the possibility of spruce-type PSII supercomplex formation in angiosperms. Unlike in spruce, both LHCB3 and LHCB6 were found to be necessary for stable binding of the M trimer to the PSII core in Arabidopsis. The formation of spruce-type PSII supercomplex was observed with low abundance only in Ihcb3 plants, suggesting a unique structural arrangement in this mutant.
Article
Plant Sciences
Ladislav Nedbal, Dusan Lazar
Summary: Frequency-domain measurements of plant responses to sinusoidally modulated light can provide valuable information without the need for dark acclimation, targeting different kinetic domains and regulatory feedbacks through analysis of amplitudes, phase shifts, and upper harmonic modulation for a wide range of frequencies. This approach can be a versatile tool in plant sensing, reflecting nonlinear phenomena such as photosynthetic regulation.
Review
Plant Sciences
Alexandrina Stirbet, Lars Olof Bjorn, Dmitry Shevela, Suleyman Allakhverdiev, Arthur Nonomura, Xin-Guang Zhu, Dusan Lazar, Ashwani Pareek, Gyozo Garab, Julian J. Eaton-Rye
NEW ZEALAND JOURNAL OF BOTANY
(2020)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)