Article
Engineering, Environmental
Jiaming Lily Shi, William A. Mitch
Summary: Chlorine reactions with peptide-bound amino acids contribute to pathogen inactivation by degrading protein structure and function, and forming disinfection byproducts. This study characterized the reactions of lysine and arginine with chlorine, demonstrating the formation of chloramines as the major products. The chloramines formed nitriles and aldehydes, which are more relevant than the corresponding aldehydes and nitriles to byproduct formation and pathogen inactivation over timescales relevant to drinking water distribution. Previous research has shown that lysine chloramines are cytotoxic and genotoxic, and their conversion to neutral chloramines alters protein structure and function, enhancing protein aggregation and contributing to pathogen inactivation.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sathish Kumar Marimuthu, Krishnanand Nagarajan, Sathish Kumar Perumal, Selvamani Palanisamy, Latha Subbiah
Summary: Host defense peptides or antimicrobial peptides (AMPs) are short sequences of amino acids with positive or negative charges that exhibit broad-spectrum antibacterial, antiviral, and antifungal activity. AMPs rich in tryptophan, arginine, and proline are considered promising sources for the development of next-generation antibiotics. This study analyzed and classified 2924 AMPs based on their amino acid sequences and investigated their structures and stability using modeling and simulations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Vanesa Lafarga, Oleksandra Sirozh, Irene Diaz-Lopez, Antonio Galarreta, Misaru Hisaoka, Eduardo Zarzuela, Jasminka Boskovic, Bogdan Jovanovic, Rafael Fernandez-Leiro, Jaime Munoz, Georg Stoecklin, Ivan Ventoso, Oscar Fernandez-Capetillo
Summary: The presence of arginine-rich cell-penetrating peptides (CPPs) leads to displacement of RNA- and DNA-binding proteins from chromatin and mRNA, affecting nucleic acid-related reactions, potentially explaining the toxicity of these CPPs. The effects of (PR)n dipeptide repeat were found to be similar to those of protamine, a small arginine-rich protein that displaces histones during spermatogenesis.
Article
Biology
Yamato Takeuchi, Kazunori Ushimaru, Kohei Kaneda, Chitose Maruyama, Takashi Ito, Kazuya Yamanaka, Yasushi Ogasawara, Hajime Katano, Yasuo Kato, Tohru Dairi, Yoshimitsu Hamano
Summary: This study reports for the first time the direct penetration of cell membranes by bacteria-derived polycationic homopoly(amino acid)s, which deliver proteins of interest into cells. This finding is of significant importance for understanding the biological significance of polycationic homopoly(amino acid)s and their potential in cellular internalization.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Physical
Sydney C. Povilaitis, Lauren J. Webb
Summary: Different anionic lipid headgroups have different effects on the insertion process of cationic membrane-permeating peptides. Anionic lipids in the outer leaflet enhance peptide-membrane insertion, while those in the inner leaflet have no significant effect.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Biochemical Research Methods
Qi Wang, Zhouxian Li, Jiahua Zhou, Yan Wang, Keyun Wang, Hongqiang Qin, Mingliang Ye
Summary: Protein methylation, especially on arginine and lysine residues, plays a critical role in cellular processes such as RNA splicing and DNA repair. A new chemical strategy has been developed to deplete histidine-containing peptides and improve the identification of low-abundance arginine/lysine methylpeptides. This method led to a 50% increase in methylation identifications, with a total of 333 forms identified in this study.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Chemistry, Medicinal
Nikolai F. Myasoedov, Lyudmila A. Lyapina, Lyudmila A. Andreeva, Marina E. Grigorieva, Tamara Y. Obergan, Tatiana A. Shubina
Summary: The study found that glyproline peptides have a complex effect on fat and carbohydrate metabolism and hemostasis system in animals, increasing antiplatelet, anticoagulant, and fibrinolytic activity of blood plasma. These compounds also exhibit normoglycemic and normolipidemic effects, leading to a slowdown or decrease in body weight growth.
MEDICINAL RESEARCH REVIEWS
(2021)
Article
Spectroscopy
Tianran Wang, Qidan Pang, Zhipu Tong, Hanyue Xiang, Nao Xiao
Summary: A simple probe BHN based on naphthol and benzothiazole has been reported for detecting arginine and lysine with high selectivity and sensitivity, along with fluorescence turn-on sensing. The probe is not sensitive to other common amino acids. The sensing mechanism has been proposed through pH investigation and H-1 NMR spectra.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Microbiology
Maria Anto Dani Nishanth, Sirsant Bhoomika, Diksha Gourkhede, Bhargavi Dadimi, Jess Vergis, Satya Veer Singh Malik, Sukhadeo Baliram Barbuddhe, Deepak Bhiwa Rawool
Summary: The study demonstrates that the in-house designed CAMA-CPP has superior antibacterial efficacy and minimal adverse effects. CAMA-CPP shows better stability and lower cytotoxicity compared to other CPPs, and exhibits significant effectiveness against multidrug-resistant Salmonella infections.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karen Johanna Cardenas-Martinez, Derly Grueso-Mariaca, Yerly Vargas-Casanova, Laura Bonilla-Velasquez, Sandra Monica Estupinan, Claudia Marcela Parra-Giraldo, Aura Lucia Leal, Zuly Jenny Rivera-Monroy, Javier Eduardo Garcia-Castaneda
Summary: Short peptides derived from Lys-LfcinB showed similar antibacterial activity to Arg peptides, and could enhance the antibacterial effect of ciprofloxacin while reducing hemolytic activity. This suggests that these peptides may be a cost-effective alternative for developing drugs or combinatory treatments against bacterial infections.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2021)
Article
Agriculture, Multidisciplinary
Jing Wu, Yuanyuan Sun, Xiang Huan, Chun Cui
Summary: Some arginyl dipeptides and gamma-glutamyl peptides have been identified as salt and umami enhancers. In this study, a series of gamma-glutamyl peptides containing Arg were synthesized and evaluated for their taste-enhancing effects. The results showed that certain peptides had significant effects in increasing both saltiness and umaminess, especially at a high solid concentration of 30%.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Engineering, Environmental
Nadia A. Samak, Mohamed S. Selim, Zhifeng Hao, Jianmin Xing
Summary: In this study, cyclic dodecapeptides rich in Arg-Trp were synthesized and immobilized on an RGO/MnO2 nanocomposite, showing excellent antimicrobial activity and ability to degrade biofilms.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Minghai Ma, Ruizhao Zhao, Xing Li, Minxuan Jing, Rundong Song, Jinhai Fan
Summary: Cell-penetrating peptides (CPPs) are short peptides of fewer than 30 amino acids, rich in arginine or lysine. They have been studied for their ability to deliver various cargos, such as drugs and nucleic acids, due to their high transmembrane efficiency and endosome escape capabilities. In this review, we summarize the function, design principles, and penetrating mechanisms of arginine-rich CPPs, and discuss their biomedical applications in drug delivery and biosensing in tumors.
CURRENT DRUG DELIVERY
(2023)
Article
Cell Biology
Omar G. Klinsky, Paula A. Wetten, Emilia Zanni-Ruiz, Martin A. Pavarotti, Maria Victoria Berberian, Marcela A. Michaut
Summary: In this study, the potential of cell-penetrating peptides (CPP) as biotechnological tools for delivering molecules into oocytes was assessed, and the functionality of permeable tetanus toxin during cortical reaction was evaluated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Ludimila Leite Marzochi, Caroline Izak Cuzziol, Carlos Henrique Viesi Do Nascimento Filho, Juliana Amorim dos Santos, Marcia Maria Urbanin Castanhole-Nunes, Eliete Neves Silva Guerra, Eny Maria Goloni-Bertollo
Summary: Histone modifications play a role in cancer initiation and progression, and targeting these proteins has potential therapeutic value. This review summarizes the development and prospects of histone methyltransferase (HMT) inhibitors in cancer therapy. Several HMT inhibitors have shown significant anti-tumor effects in clinical studies, with tazemetostat being the most advanced in follicular lymphoma treatment. The use of HMT inhibitors has produced promising results in hematological cancers with manageable adverse effects. Ongoing clinical studies suggest a hopeful future for HMT inhibitors in treating different types of cancer.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Chemistry, Physical
Johann Hansing, Catrin Ciemer, Won Kyu Kim, Xiaolu Zhang, Jason E. DeRouchey, Roland R. Netz
EUROPEAN PHYSICAL JOURNAL E
(2016)
Article
Polymer Science
Nathanael A. Stocke, Xiaolu Zhang, J. Zach Hilt, Jason E. DeRouchey
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2017)
Article
Polymer Science
Min An, Gulen Yesilbag Tonga, Sean R. Parkin, Vincent M. Rotello, Jason E. DeRouchey
Article
Chemistry, Multidisciplinary
Johann Hansing, Joseph R. Duke, Emily B. Fryman, Jason E. DeRouchey, Roland R. Netz
Article
Biochemistry & Molecular Biology
Logan W. Warriner, Joseph R. Duke, Daniel W. Pack, Jason E. DeRouchey
Article
Biochemical Research Methods
Xu Fu, Yongwook Song, Abdullah Masud, Kanthi Nuti, Jason E. DeRouchey, Christopher I. Richards
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Christian D. Powell, Daniel C. Kirchoff, Jason E. DeRouchey, Hunter N. B. Moseley
Article
Toxicology
Wesley N. Saintilnord, Sara Y. N. Tenlep, Joshua D. Preston, Eleonora Duregon, Jason E. DeRouchey, Jason M. Unrine, Rafael de Cabo, Kevin J. Pearson, Yvonne N. Fondufe-Mittendorf
Summary: Chronic cadmium exposure was found to induce differential DNA methylation changes in mouse sperm, with most changes correlating positively with alterations in gene expression. The targeted genes by cadmium exposure are involved in critical developmental processes. This study provides a foundational framework for investigating potential effects on fertility and offspring through paternal inheritance.
TOXICOLOGICAL SCIENCES
(2021)
Article
Medicine, Research & Experimental
Nasir Uddin, Logan W. Warriner, Daniel W. Pack, Jason E. DeRouchey
Summary: Gene therapy has advanced significantly, with the use of polymeric materials as gene delivery scaffolds showing increased gene expression and gene editing efficiency in the presence of serum.
MOLECULAR PHARMACEUTICS
(2021)
Article
Cell Biology
Samir P. Patel, Felicia M. Michael, M. Arif Khan, Brian Duggan, Sam Wyse, Daniel R. Darby, Krishnaroop Chaudhuri, Jonathan T. Pham, Jenna Gollihue, Jason E. DeRouchey, Patrick G. Sullivan, Tom D. Dziubla, Alexander G. Rabchevsky
Summary: A thermal-gelling, erodible hydrogel system was developed for localized delivery of viable mitochondria in vivo, and the effects of transplanted mitochondria on cellular metabolism were investigated. The respirator capacity of hydrogel-released mitochondria was higher than those without gel, and the labeling of mitochondria was reversible. The hydrogel protected mitochondria in vitro and solidified locally within 60 minutes.
Article
Chemistry, Multidisciplinary
Hossain Shadman, Caleb Edward Gallops, Jesse D. Ziebarth, Jason E. DeRouchey, Yongmei Wang
Summary: The structures of protamines in salmon, bull, and human were examined using molecular dynamics simulations. The protamines exhibited a wide range of sizes and shapes, indicating disorder, but also formed secondary structures. The results support a model for eutherian protamine structures where a highly charged center is surrounded by disulfide-bond-stabilized loops.
Article
Biochemistry & Molecular Biology
Richard J. J. Mitchell, Sarah M. M. Kriger, Alexander D. D. Fenton, Dmytro Havrylyuk, Ankit Pandeya, Yang Sun, Tami Smith, Jason E. E. DeRouchey, Jason M. M. Unrine, Viral Oza, Jessica S. S. Blackburn, Yinan Wei, David K. K. Heidary, Edith C. C. Glazer
Summary: A potent Ru(ii) agent with cytotoxicity in cancer cells and minimal toxicity in zebrafish embryos has been designed as a monofunctional anticancer agent, mimicking the biological effects of phenanthriplatin.
RSC CHEMICAL BIOLOGY
(2023)
Meeting Abstract
Cell & Tissue Engineering
F. M. Michael, S. P. Patel, H. M. Vaught, A. Khan, P. G. Sullivan, J. DeRouchey, T. D. Dziubla, A. G. Rabchevsky
CELL TRANSPLANTATION
(2021)
Article
Chemistry, Multidisciplinary
Stuart S. Lichtenberg, Kanthi Nuti, Jason DeRouchey, Olga Tsyusko, Jason M. Unrine
ENVIRONMENTAL SCIENCE-NANO
(2020)
Article
Biophysics
James M. Hutchison, Donald C. Rau, Jason E. DeRouchey
BIOPHYSICAL JOURNAL
(2017)