Article
Biochemistry & Molecular Biology
Jordan D. Pritts, Sarah L. J. Michel
Summary: Metal ions are commonly found as protein co-factors in biology. The distribution and utilization of metals in biology has evolved over time. Zinc finger proteins are a common class of metal co-factored proteins, but there have been cases where proteins annotated as zinc finger proteins actually contain Fe-S clusters. Researchers can differentiate the metal co-factors using bioinformatics and specific methods.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Leszek Rydz, Maria Wrobel, Halina Jurkowska
Summary: Mitochondria are crucial organelles for the synthesis of Fe-S clusters, with sulfurtransferases playing a key role. Fe-S clusters are essential for Fe-S proteins, susceptible to oxidative stress but capable of being resynthesized or repaired. Sulfurtransferases are involved in the formation, maturation, and reconstitution of Fe-S clusters.
Article
Biochemistry & Molecular Biology
Daniel W. Bak, Eranthie Weerapana
Summary: This study describes a chemoproteomic strategy that monitors changes in the reactivity of Fe-S cysteine ligands to inform on Fe-S cluster occupancy and demonstrates its utility in Escherichia coli. The results show global disruptions in Fe-S incorporation in cells cultured under iron-depleted conditions, identify Fe-S client proteins reliant on scaffold, carrier, and chaperone proteins within the Isc Fe-S biogenesis pathway, and identify two previously unannotated Fe-S proteins, TrhP and DppD.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Genetics & Heredity
Nidhi Kirtikumar Bub, Sakshi Anand, Swati Garg, Vishal Saxena, Dhanabala Subhiksha Rajesh Khanna, Deeptanshu Agarwal, Sanjay Kumar Kochar, Shailja Singh, Shilpi Garg
Summary: This study identifies Dre2 as a potential target for artemisinin drugs and demonstrates that artemisinin derivatives can interact with Dre2, altering its activity and inducing cell death.
Article
Polymer Science
Hadia Baloch, Aisha Siddiqua, Asif Nawaz, Muhammad Shahid Latif, Syeda Qurbat Zahra, Suliman Yousef Alomar, Naushad Ahmad, Tarek M. Elsayed
Summary: The aim of this study was to synthesize sulfur nanoparticles (SNPs) from Citrus limon leaves that are non-noxious, clean, reliable, and green. The synthesized SNPs were characterized for their particle size, surface charge, and functional properties. The SNPs exhibited a globule size of 55.32 +/- 2.15 nm, PDI value of 0.365 +/- 0.06, and zeta potential of -12.32 +/- 0.23 mV. Antimicrobial and antifungal studies showed that the SNPs had better activity against various strains at a minimal inhibitory concentration of 50 μg/mL. Additionally, embedding the SNPs in a nanohydrogel formulation showed promising results in wound healing studies.
Article
Chemistry, Inorganic & Nuclear
Juan He, Jia Wei, Gan Xu, Xu-Dong Chen
Summary: The rational construction of versatile Mo-Fe-S cubane clusters was achieved using a LEGO strategy, providing a controlled synthesis method for biomimetic chemistry.
INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Melissa Jansing, Steffen Mielenbrink, Hannah Rosenbach, Sabine Metzger, Ingrid Span
Summary: This study compares the effects of three different maturation strategies on three well-established [4Fe-4S] proteins. The results show that co-expression of the genes for iron-sulfur cluster biogenesis pathway can improve protein yield and cluster content. In vivo maturation can reduce the formation of iron-sulfur aggregates that commonly occur in chemical reconstitution. The study also demonstrates the potential of using in vivo maturation strategies for proteins previously only matured by chemical reconstitution.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ingie Elchennawi, Philippe Carpentier, Christelle Caux, Marine Ponge, Sandrine Ollagnier de Choudens
Summary: Iron-sulfur (Fe-S) clusters are essential for cellular pathways and require proteins to mobilize sulfur and iron for their assembly. The SUF system in Mycobacterium tuberculosis is the primary Fe-S biogenesis system and a potential target for fighting tuberculosis. In this study, two proteins of the Mtb SUF system, Rv1464(sufS) and Rv1465(sufU), were characterized and their interaction and activities in Fe-S biogenesis were investigated.
Article
Microbiology
Mathieu Sourice, Isabel Askenasy, Pierre Simon Garcia, Yann Denis, Gael Brasseur, Patricia J. J. Kiley, Beatrice Py, Corinne Aubert
Summary: Fe-S proteins play a crucial role in various biological processes, making it essential for organisms to maintain Fe-S cluster homeostasis. This study presents the coordination of two Fe-S cluster biogenesis machineries in a bacterium from the Deltaproteobacteria branch. The findings highlight the diversity and commonalities in the regulation of Fe-S cluster homeostasis in response to environmental changes.
Article
Biochemistry & Molecular Biology
Parool Gupta, Rani Mansuri, Priya Priydarshni, Sachidananda Behera, Amir Zaidi, Shamshun Nehar, Ganesh Chandra Sahoo, Krishna Pandey, Vahab Ali
Summary: This study revealed the interaction between two proteins, Cfd1 and Nbp35, in L. donovani, forming a stable complex involved in the cellular process of Fe-S cluster biogenesis and transfer. The upregulation of Cfd1 and Nbp35 proteins in drug-resistant parasites leads to increased Fe-S protein levels, promoting parasite survival under drug pressure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Infectious Diseases
Mengyu Zhao, Shuang He, Renqiao Wen, Chao Li, Xinggui Chen, Xiaolong Lin, Hongning Wang, Yizhi Tang
Summary: This study is the first to report the MV-mediated co-transfer of ARG-carrying plasmids and transposons in the Gram-positive bacterium E. faecium.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Plant Sciences
Jonathan Przybyla-Toscano, Andrew E. Maclean, Marina Franceschetti, Daniela Liebsch, Florence Vignols, Olivier Keech, Nicolas Rouhier, Janneke Balk
Summary: NFU4 and NFU5 proteins in plants have a housekeeping function and are involved in the lipoylation process of mitochondrial dehydrogenases, providing Fe-S clusters to lipoyl synthase.
Article
Oncology
Sylvain Garciaz, Andrew A. Guirguis, Sebastian Muller, Fiona C. Brown, Yih-Chih Chan, Ali Motazediani, Caitlin L. Rowe, James A. Kuzich, Kah Lok Chan, Kevin Tran, Lorey Smith, Laura MacPherson, Brian Liddicoat, Enid Y. N. Lam, Tatiana Caneque, Marian L. Burr, Veronique Litalien, Giovanna Pomilio, Mathilde Poplineau, Estelle Duprez, Sarah-Jane Dawson, Georg Ramm, Andrew G. Cox, Kristin K. Brown, David C. S. Huang, Andrew H. Wei, Kate McArthur, Raphael Rodriguez, Mark A. Dawson
Summary: Ironomycin is a drug that disrupts mitochondrial metabolism and induces cell death in cancer cells by reducing mitochondrial iron load and activating BAX/BAK. It exhibits synergy with BH3 mimetics and overcomes venetoclax resistance in cancers such as acute myeloid leukemia.
Article
Engineering, Environmental
Qinghong Sui, Lanlan Liu, Lingyu Hu, Yujun Zhou, Jiansheng Li, Lixiang Zhou, Di Fang
Summary: This study compared the roles of S0-oxidizing bacteria (S0OB) and S0-reducing bacteria (S0RB) in releasing insoluble phosphorus from anaerobically digested sludge (ADS). The results showed that S0OB treatment extracted more phosphate from the sludge compared with S0RB treatment, but it also released a considerable amount of metal cations and negatively affected sludge dewaterability. Ultimately, 79.8wt% of phosphorus was recovered from ADS through S0OB extraction-CER purification-alkali neutralization-vivianite crystallization.
Article
Multidisciplinary Sciences
Nunziata Maio, Bernard A. P. Lafont, Debangsu Sil, Yan Li, J. Martin Bollinger, Carsten Krebs, Theodore C. Pierson, W. Marston Linehan, Tracey A. Rouault
Summary: Research has shown that the catalytic subunit nsp12 of the SARS-CoV-2 RdRp complex ligates two iron-sulfur metal cofactors which are essential for viral replication and interaction with the viral helicase. Oxidation of these clusters by TEMPOL inhibits RdRp activity and blocks SARS-CoV-2 replication in cell culture, indicating that these iron-sulfur clusters could be targeted for therapy of COVID-19.
Article
Microbiology
Katherine A. Black, Patricia C. Dos Santos
JOURNAL OF BACTERIOLOGY
(2015)
Article
Biochemistry & Molecular Biology
William H. Clodfelter, Swati Basu, Crystal Bolden, Patricia C. Dos Santos, S. Bruce King, Daniel B. Kim-Shapiro
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2015)
Editorial Material
Genetics & Heredity
Dennis R. Dean, Patricia C. Dos Santos
Article
Chemistry, Multidisciplinary
Simon J. George, Jose A. Hernandez, Emilio Jimenez-Vicente, Carlos Echavarri-Erasun, Luis M. Rubio
CHEMICAL COMMUNICATIONS
(2016)
Article
Chemistry, Inorganic & Nuclear
Song Gao, Simon J. George, Zhao-Hui Zhou
DALTON TRANSACTIONS
(2016)
Review
Biochemistry & Molecular Biology
Chenkang Zheng, Patricia C. Dos Santos
BIOCHEMICAL SOCIETY TRANSACTIONS
(2018)
Article
Biochemistry & Molecular Biology
Chenkang Zheng, Selina Guo, William G. Tennant, Pradyumna K. Pradhan, Katherine A. Black, Patricia C. Dos Santos
Article
Biochemistry & Molecular Biology
Maame A. Addo, Patricia C. Dos Santos
Article
Physics, Applied
Simon J. George, Matthew H. Carpenter, Stephan Friedrich, Robin Cantor
JOURNAL OF LOW TEMPERATURE PHYSICS
(2020)
Review
Genetics & Heredity
Ashley M. Edwards, Maame A. Addo, Patricia C. Dos Santos
Article
Instruments & Instrumentation
Linda Vogt, Julien J. H. Cotelesage, Charles J. Titus, Samin Sharifi, Albert E. Butterfield, Peter Hillman, Ingrid J. Pickering, Graham N. George, Simon J. George
Summary: Oxygen K-edge X-ray absorption spectroscopy is commonly used for studying solid materials, but studying liquid samples faces challenges of high-vacuum conditions and oxygen contamination. A new modular sample holder design allows for encapsulating liquid samples under high-vacuum conditions and provides a protocol for minimizing background oxygen contamination.
JOURNAL OF SYNCHROTRON RADIATION
(2021)
Article
Microbiology
Ashley M. Edwards, Katherine A. Black, Patricia C. Dos Santos
Summary: This study reveals that in the model Gram-positive organism Bacillus subtilis, the levels of s(2)U are responsive to sulfur availability. Downregulation of the s(2)U biosynthetic components leads to lower s(2)U levels, which may serve as a signal for the slowing of the translational apparatus during cellular nutrient insufficiency.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Julia S. Martin del Campo, Jack Rigsbee, Marcelo Bueno Batista, Florence Mus, Luis M. Rubio, Oliver Einsle, John W. Peters, Ray Dixon, Dennis R. Dean, Patricia C. Dos Santos
Summary: Understanding the reduction of nitrogen gas to ammonia at ambient conditions has been a longstanding challenge. This knowledge is crucial for transferring biological nitrogen fixation to crops and developing improved synthetic catalysts. Azotobacter vinelandii has emerged as the preferred model organism for studying this process over the past 30 years. This review summarizes the current understanding and places it in historical context.
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Linda Vogt, Julien J. H. Cotelesage, Natalia Dolgova, Charles J. Titus, Samin Sharifi, Simon J. George, Ingrid J. Pickering, Graham N. George
Review
Biochemistry & Molecular Biology
Chenkang Zheng, Katherine A. Black, Patricia C. Dos Santos