Article
Agriculture, Multidisciplinary
Elisabeth Koch, Michelle Wiebel, Ariane Loewen, Ina Willenberg, Nils Helge Schebb
Summary: Enzymatic and nonenzymatic oxidation of linoleic (LA) and alpha-linolenic acid (ALA) during pressing and storage of plant oils leads to the formation of various oxylipins. The concentrations of oxylipins are found to be correlated with the peroxide value (PV) of the oils, while secondary volatile aldehydes do not show similar correlations. Comprehensive analysis of different types of hydroxy-, epoxy-, and dihydroxy-LA/-ALA provides new insights into the composition of plant oils and ongoing oxidation processes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agronomy
Grace H. H. Liang, Orpheus M. M. Butler, Charles R. R. Warren
Summary: Plants and soil microbes can reduce their phosphorus requirements by replacing phospholipids with non-phosphorus lipids in response to phosphorus deficiency. However, there have been few studies on this process in natural ecosystems, making it unclear whether this replacement occurs across soil phosphorus availability gradients.
Article
Nutrition & Dietetics
Guozhu Ye, Bi-cheng Yang, Han Gao, Zeming Wu, Jinsheng Chen, Xiao-yan Ai, Qiansheng Huang
Summary: This study found that oleate induced disorders of macrophage phospholipid metabolism, highlighting their potential as therapeutic targets for intervening diet-related metabolic diseases.
JOURNAL OF NUTRITION
(2021)
Review
Nutrition & Dietetics
Filippo Egalini, Ornella Guardamagna, Giulia Gaggero, Emanuele Varaldo, Beatrice Giannone, Guglielmo Beccuti, Andrea Benso, Fabio Broglio
Summary: Massive changes in diet have led to an imbalance between omega-3 and omega-6 fatty acids, mainly due to the increased consumption of omega-6-rich vegetable oils and depletion of omega-3 in our food. Previous studies have shown conflicting results regarding the effects of omega-3 and omega-6 on glucose metabolism, with factors such as source of omega-3, sample size, ethnicity, study duration, and food cooking method playing a role. A high EPA/AA ratio appears to be a promising indicator of better glycemic control and reduced inflammation, while linoleic acid may also be associated with a lower incidence of type 2 diabetes mellitus.
Article
Biochemistry & Molecular Biology
Aike Jeucken, Miaomiao Zhou, Marc M. S. M. Wosten, Jos F. Brouwers
Summary: n-Butanol is a versatile compound that shows promise as a biofuel, with potential applications in producing complex molecules like bioplastics. This study investigated the impact of n-butanol on E. coli lipid metabolism, identifying key lipids and pathways influencing n-butanol tolerance. The research provides new insights into bacterial lipid metabolism.
Article
Biochemistry & Molecular Biology
Haixing Wang, Siyan Chen, Zhao Han, Ting Li, Jianfeng Ma, Xi Chen, Jie Pang, Qingcheng Wang, Qing Shen, Manman Zhang
Summary: A sensitive and accurate lipidomic method was developed to identify and quantify phospholipids in plasma. The method successfully determined 31 molecular species of phospholipids and identified 10 biomarkers that showed significant differences between different subtypes of stroke and healthy controls. The study also found that oleic acid-enriched phospholipids were positively related to stroke and may be potential risk factors in the future.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hyun Jun Jang, Kyoung Ja Kwon, Chan Young Shin, Ga Seul Lee, Jeong Hee Moon, Tae Geol Lee, Sohee Yoon
Summary: Autism is associated with changes in polyunsaturated fatty acid (PUFA) levels, suggesting a link between lipid metabolism and autism spectrum disorder (ASD). In this study, phospholipid distribution in the brain tissue of autistic mice was investigated using MALDI-MSI, revealing differences in phospholipids containing important PUFAs for cell signaling and brain growth. The identification of these differences will contribute to a better understanding of changes in lipid metabolism in ASD, when combined with traditional biological fluid analysis.
Article
Chemistry, Analytical
Xiujun Cao, Yuan Luo, Xueli Liu, Chunqing Shang, Jun Lu, Guoxin Song, Chunhui Deng
Summary: Hirudin, a natural substance found in leeches, has both therapeutic and toxic effects. This study proposes a new method for detecting hirudin in serum using affinity magnetic microspheres. The method combines liquid chromatography and mass spectrometry analysis to achieve accurate detection. The method has a low limit of detection, good linearity, and acceptable precision.
Article
Biochemical Research Methods
Haixing Wang, Ting Li, Qing Shen, Zhifang Liu, You-Wei Chen, Darong Huang, Jiancheng Yu, Long Wang
Summary: Liver cancer is the leading cause of cancer deaths worldwide, with hepatocellular carcinoma (HCC) accounting for over 90% of cases. This study optimized a mass spectrometry technique called rapid evaporative ionization mass spectrometry (REIMS) for analyzing HCC tumor tissue. Through the analysis of 12 human HCC tumor tissue specimens, it was found that certain free fatty acids and phospholipids were distributed differently in HCC, paracancerous tissue (PT), and noncancerous liver tissue (NCT). The upregulation of specific fatty acids and phospholipids was identified in HCC specimens, and a statistical model was established for real-time HCC tumor diagnosis with high sensitivity and specificity.
Review
Spectroscopy
Mikael Fabritius, Baoru Yang
Summary: Analysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be done through direct separation or quantification of regioisomer ratios using mass spectrometry. Direct chromatography separation is being replaced by mass spectrometry due to limitations. However, challenges arise from the large number of isobaric and isomeric lipid species in natural samples and the lack of available regiopure standards. Optimization algorithms and fragmentation models are useful for analysis of TG regioisomers.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Chemistry, Applied
Qing Shen, Gongshuai Song, Honghai Wang, Yanping Zhang, Yiwei Cui, Hujun Xie, Jing Xue, Haixing Wang
Summary: Shrimp waste is a rich source of bioactive lipids, and the 1,2-dichloroethane method for extracting phospholipids shows comparable results to traditional methods. Different extraction methods significantly impact the lipidomics profile of the shrimp waste extract, but the diversity and percentage of molecular species remain generally consistent.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Engineering, Environmental
Jing He, Hong Zhang, Yingxue Ma, Yuwei He, Zhuo Liu, Junyu Liu, Sheng Wang, Yaqi Liu, Kai Yu, Jie Jiang
Summary: This paper investigates the impact of salt cations in sea spray aerosols (SSAs) on the ozonolysis of model lung phospholipids at the air-water interface. The results show that salt cations facilitate the interfacial ozonolysis of phospholipids, and this facilitation is dependent on the concentration, charge number, and ion radius of the salt cations. The mechanism study reveals that the electrostatic interaction between the phospholipids and the salt cations disrupts the packing of the phospholipids, making the oleyl chains more susceptible to ozone. The aerosolization of salt-dominated artificial seawater and real seawater also demonstrates a significant increase in the ozonolysis of phospholipids intervened by salt cations.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Ke Wang, Xiao-Ping Peng, Dan Feng, Chuan Tian, Wen-Long Wan, Hong-Xiang Lou, Gang Li
Summary: In this study, a variety of GAs compounds were isolated from a fungus and their spectroscopic data were reported. Compound 1 showed a promoting effect on the growth of Arabidopsis thaliana seedlings.
Article
Pharmacology & Pharmacy
Valentina Shpakova, Natalia Rukoyatkina, Nada Al Arawe, Anna Prilepskaya, Alexandra Kharazova, Iraida Sharina, Stepan Gambaryan, Emil Martin
Summary: This study reveals the molecular mechanisms of the 12-LOX inhibitor ML355 on platelets, demonstrating its antiplatelet and protective effects, which may be significant for antiplatelet and anticancer therapy.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2022)
Article
Biotechnology & Applied Microbiology
Tae-Rim Choi, Suk Jin Oh, Jeong Hyeon Hwang, Hyun Jin Kim, Nara Shin, Jeonghee Yun, Sang-Ho Lee, Shashi Kant Bhatia, Yung-Hun Yang
Summary: NdgR, a global regulator in Streptomyces, regulates branched-chain amino acid metabolism by binding to synthetic genes. Its function was studied using PLFA analysis, which showed that ndgR deletion decreased isoleucine- and leucine-related fatty acids but increased valine-related fatty acids. This deletion also impaired growth at low temperatures, but supplementation of leucine and isoleucine could restore growth. NdgR was found to be involved in the control of branched-chain amino acids and membrane fatty acid composition.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Hui-wen Lue, Jennifer Podolak, Kevin Kolahi, Larry Cheng, Soumya Rao, Devin Garg, Chang-Hui Xue, Juha K. Rantala, Jeffrey W. Tyner, Kent L. Thornburg, Ann Martinez-Acevedo, Jen-Jane Liu, Christopher L. Amling, Charles Truillet, Sharon M. Louie, Kimberly E. Anderson, Michael J. Evans, Valerie B. O'Donnell, Daniel K. Nomura, Justin M. Drake, Anna Ritz, George V. Thomas
GENES & DEVELOPMENT
(2017)
Article
Immunology
Stefan Uderhardt, Jochen A. Ackermann, Tobias Fillep, Victoria J. Hammond, Johann Willeit, Peter Santer, Manuel Mayr, Markus Biburger, Meike Miller, Katie R. Zellner, Konstantin Stark, Alexander Zarbock, Jan Rossaint, Irene Schubert, Dirk Mielenz, Barbara Dietel, Dorette Raaz-Schrauder, Cihan Ay, Thomas Gremmel, Johannes Thaler, Christian Heim, Martin Herrmann, Peter W. Collins, Gernot Schabbauer, Nigel Mackman, David Voehringer, Jerry L. Nadler, James J. Lee, Steffen Massberg, Manfred Rauh, Stefan Kiechl, Georg Schett, Valerie B. O'Donnell, Gerhard Kroenke
JOURNAL OF EXPERIMENTAL MEDICINE
(2017)
Editorial Material
Biochemistry & Molecular Biology
Valerie B. O'Donnell, Robert C. Murphy
JOURNAL OF LIPID RESEARCH
(2017)
Correction
Immunology
C. A. Stokes, R. Kaur, M. R. Edwards, M. Mondhe, D. Robinson, E. C. Prestwich, R. D. Hume, C. A. Marshall, Y. Perrie, V. B. O'Donnell, J. L. Harwood, I. Sabroe, L. C. Parker
MUCOSAL IMMUNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Maceler Aldrovandi, Swathi Banthiya, Sven Meckelmann, You Zhou, Dagmar Heydeck, Valerie B. O'Donnell, Hartmut Kuhn
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2018)
Review
Biochemistry & Molecular Biology
Albert van der Vliet, Tobias P. Dick, Steve D. Aust, Willem H. Koppenol, Fulvio Ursini, Anthony J. Kettle, Joseph S. Beckman, Valerie O'Donnell, Victor Darley-Usmar, Jack Lancaster, Neil Hogg, Kelvin J. A. Davies, Henry Jay Forman, Yvonne M. W. Janssen-Heininger
FREE RADICAL BIOLOGY AND MEDICINE
(2018)
Review
Medicine, Research & Experimental
Valerie B. O'Donnell, Jamie Rossjohn, Michael J. O. Wakelam
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Correction
Immunology
Stefan Uderhardt, Jochen A. Ackermann, Tobias Fillep, Victoria J. Hammond, Johann Willeit, Peter Santer, Manuel Mayr, Markus Biburger, Meike Miller, Katie R. Zellner, Konstantin Stark, Alexander Zarbock, Jan Rossaint, Irene Schubert, Dirk Mielenz, Barbara Dietel, Dorette Raaz-Schrauder, Cihan Ay, Thomas Gremmel, Johannes Thaler, Christian Heim, Martin Herrmann, Peter W. Collins, Gernot Schabbauer, Nigel Mackman, David Voehringer, Jerry L. Nadler, James J. Lee, Steffen Massberg, Manfred Rauh, Stefan Kiechl, Georg Schett, Valerie B. O'Donnell, Gerhard Kronke
JOURNAL OF EXPERIMENTAL MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Bruce A. Freeman, Valerie B. O'Donnell, Francisco J. Schopfer
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2018)
Article
Biochemical Research Methods
Eoin Fahy, Jorge Alvarez-Jarreta, Christopher J. Brasher, An Nguyen, Jade I. Hawksworth, Patricia Rodrigues, Sven Meckelmann, Stuart M. Allen, Valerie B. O'Donnell
Editorial Material
Biochemistry & Molecular Biology
Valerie B. O'Donnell, Edward A. Dennis, Michael J. O. Wakelam, Shankar Subramaniam
Article
Medicine, Research & Experimental
David A. Slatter, Charles L. Percy, Keith Allen-Redpath, Joshua M. Gajsiewicz, Nick J. Brooks, Aled Clayton, Victoria J. Tyrrell, Marcela Rosas, Sarah N. Lauder, Andrew Watson, Maria Dul, Yoel Garcia-Diaz, Maceler Aldrovandi, Meike Heurich, Judith Hall, James H. Morrissey, Sebastien Lacroix-Desmazes, Sandrine Delignat, P. Vincent Jenkins, Peter W. Collins, Valerie B. O'Donnell
Article
Oncology
Jaqui Walker, Pippa Hutchison, Junbo Ge, Dong Zhao, Yongjun Wang, Peter M. Rothwell, J. Michael Gaziano, Andrew Chan, John Burn, John Chia, Ruth Langley, Valerie O'Donnell, Bianca Rocca, Chris Hawkey
ECANCERMEDICALSCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Jinming Zhao, Yoshinori Minami, Emily Etling, John M. Coleman, Sarah N. Lauder, Victoria Tyrrell, Maceler Aldrovandi, Valerie O'Donnell, Hans-Erik Claesson, Valerian Kagan, Sally Wenzel
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2017)
Article
Nutrition & Dietetics
Helen M. King, Allan G. Cosslett, Christopher P. Thomas, Rebecca Price-Davies
CLINICAL NUTRITION ESPEN
(2018)
