Article
Multidisciplinary Sciences
Michael Onyema Edeh, Surjeet Dalal, Ibidun Christiana Obagbuwa, B. V. V. Siva Prasad, Shalini Zanzote Ninoria, Mohd Anas Wajid, Ademola Olusola Adesina
Summary: Technology is crucial in healthcare for disease prevention and detection. This study presents a predictive model for diagnosing and detecting White Spot Disease among shrimp farmers using machine learning algorithms and visualization techniques. The achieved results show a high prediction accuracy, indicating the suitability of the model for real-time disease prediction.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Hui-Ju Huang, Sen-Lin Tang, Yuan-Chih Chang, Hao-Ching Wang, Tze Hann Ng, Rees F. Garmann, Yu-Wen Chen, Jiun-Yan Huang, Ramya Kumar, Sheng-Hsiung Chang, Shang-Rung Wu, Chih-Yu Chao, Kyoko Matoba, Iwasaki Kenji, William M. Gelbart, Tzu-Ping Ko, Hwei-Jiung (Andrew) Wang, Chu-Fang Lo, Li-Li Chen, Han-Ching Wang
Summary: Transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) techniques were used to study the morphology and pathogenesis of White spot syndrome virus (WSSV). The mature WSSV virions were found to have an oval shape without tail-like extensions. Two distinct ends, a portal cap, and a closed base, were observed in WSSV nucleocapsids. A C14 symmetric structure of the WSSV nucleocapsid was proposed based on Cryo-EM map. Immunoelectron microscopy (IEM) revealed a ring-like architecture formed by VP664 proteins, the main components of the 14 assembly units, in WSSV. Furthermore, a unique helical dissociation process of WSSV nucleocapsids was observed. Based on these findings, a novel morphogenetic pathway of WSSV was proposed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lei Liu, Li-Peng Shan, Yan Zhou, Jiong Chen
Summary: The synthesis of coumarin derivatives C2 and C7 has shown promising antiviral effects against white spot syndrome virus in Litopenaeus vannamei larvae, reducing toxicity and mortality while remaining stable in aquacultural water. These compounds have the potential for use in prevention and treatment of WSSV infection in shrimp seedling culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jie Gao, Ye-Jia Song, Hao Wang, Bao-Rui Zhao, Xian-Wei Wang
Summary: White spot syndrome virus (WSSV) utilizes host autophagy and lipid metabolism for reproduction, causing a serious impact on global shrimp farming. This study reveals the mechanism by which WSSV exploits host machinery for infection and provides targets for WSSV prevention and control.
Article
Fisheries
Xupeng Li, Sheng Luan, Kun Luo, Baoxiang Cao, Baolong Chen, Jie Kong, Xianhong Meng
Summary: White spot syndrome virus (WSSV) is a lethal virus in shrimp aquaculture, with Chinese shrimp being more susceptible to infection. Currently, there is no effective solution to the WSSV problem in Chinese shrimp aquaculture. Research shows that different individuals from the same family can carry varying viral loads after WSSV infection. Transcriptomic analysis revealed differentially expressed genes related to binding function and the extracellular matrix-receptor interaction pathway, suggesting their importance in the anti-WSSV processes in Chinese shrimp.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
M. G. Pratapa, Saurav Kumar, M. K. Bedekar, H. Sanath Kumar, K. V. Rajendran
Summary: This study investigates the susceptibility of mud crabs to white spot syndrome virus (WSSV) compared to shrimp. The results show that mud crabs have relatively lower mortality rates when infected with WSSV compared to shrimp. The viral load in different tissues also varies between shrimp and mud crabs.
Article
Fisheries
Xiao-Tong Cao, Gang Lu, Lian-Jie Wu, Jiang-Feng Lan
Summary: This study reveals the crucial role of Snail2 alpha and Snail2 beta in the immune response of crayfish against White spot syndrome virus (WSSV). The research shows that WSSV induces the transcription of immediate-early genes ie1 and ie15 through the nuclear translocation of Snail2 alpha and Snail2 beta. However, crayfish responds to WSSV escape by reducing the expression of these two proteins.
Article
Fisheries
Warapond Wanna, Chitchanok Aucharean, Panmile Kaitimonchai, Wachira Jaengkhao
Summary: This study investigated the effect of Pediococcus pentosaceus MR001 on the resistance to white spot disease (WSD) and the intestinal microbiota of Litopenaeus vannamei. The results showed that probiotic supplementation significantly increased the survival rate of shrimp and upregulated immunity-related genes. P. pentosaceus MR001 also altered the gut microbiota, increasing the abundance of potential probiotics and decreasing the abundance of potential pathogens.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Kejia Weng, Hongliang Zuo, Zhiming Zhu, Zhixun Guo, Shaoping Weng, Jianguo He, Xiaopeng Xu
Summary: The NF-KB signaling pathway plays a crucial role in the immune response of shrimp, while Ftz-F1H is involved in antiviral response, promoting WSSV infection by inhibiting the activation of the Dorsal pathway.
Article
Fisheries
Warumporn Yingsunthonwattana, Wisarut Junprung, Premruethai Supungul, Anchalee Tassanakajon
Summary: The study investigated the involvement of Litopenaeus vannamei (Lv) HSP90 in white spot syndrome virus (WSSV) infections. Silencing LvHSP90 resulted in reduced shrimp mortality and viral replication, but also induced apoptosis and potentially led to shrimp death.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Qingna Lai, Chen Yu, Furong Zhao, Yunying Cheng, Qin Zheng, Changkun Pan, Zhenyue Lin, Suping Yang, Qian Zhang, Jianming Chen, Wei Wang
Summary: Nitric oxide plays a crucial role in immune defense against WSSV infection in shrimp, inhibiting viral replication and reducing mortality. This study provides theoretical support for the development of NO-based therapeutic strategies.
Article
Environmental Sciences
Guo-Juan Niu, Ming Yan, Cang Li, Peng-yuan Lu, Zhongtang Yu, Jin-Xing Wang
Summary: In this study, the effects of white spot syndrome virus (WSSV) infection on the microbiota in the gastrointestinal tract of kuruma shrimp were investigated. The results showed that WSSV infection decreased microbial diversity and affected the composition of bacteria in the stomachs, but not in the intestines. Firmicutes increased significantly, while Actinobacteria, Bacteroidetes, and Cyanobacteria decreased in the stomachs of WSSV-infected shrimp.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Fisheries
Parisa Etedali, Mandana Behbahani, Hassan Mohabatkar, Ghasem Dini
Summary: A rapid colorimetric aptasensor with high selectivity and sensitivity was developed for the detection of white spot syndrome virus (WSSV). The designed aptamer showed good specificity to WSSV and could detect as low as 10^4 copies of WSSV using naked eyes. This study demonstrated excellent potential for the rapid visual colorimetric detection of WSSV in aquaculture shrimp farming.
Article
Fisheries
Wattana Weerachatyanukul, Charoonroj Chotwiwatthanakun, Pitchanee Jariyapong
Summary: The encapsulation of double stranded RNA into VLPs shows promise in combating shrimp viral infections, with co-encapsulation of dual dsRNA into IHHNV-VLPs demonstrating superior silencing ability against WSSV.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Yen Siong Ng, Der-Yen Lee, Chun-Hung Liu, Cheng-Yi Tung, Shu-Ting He, Han-Ching Wang
Summary: This study found that white spot syndrome virus (WSSV) enhances glycolysis by activating the glycolytic enzymes Hexokinase (HK) and Phosphofructokinase (PFK) in shrimp hemocytes during the viral genome replication stage, providing energy and biomolecules for virus replication.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Yuttapong Thawornwattana, Ha Thanh Dong, Kornsunee Phiwsaiya, Pakkakul Sangsuriya, Saengchan Senapin, Pakorn Aiewsakun
Summary: Tilapia lake virus (TiLV) is an emerging virus that rapidly spread worldwide over the past 6 years, its origin and epidemiology characteristics are still largely unknown.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Fisheries
Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin
Summary: Accuracy is key in PCR diagnostics, with inconsistent results observed when changing Taq DNA polymerase brands. Testing of 6 primer sets for aquaculture viruses showed differences in specificity and sensitivity, highlighting the potential improvement of detection sensitivity by adding ammonium sulfate to reaction buffers.
AQUACULTURE INTERNATIONAL
(2021)
Article
Fisheries
Nguyen Dinh-Hung, Pattiya Sangpo, Thanapong Kruangkum, Pattanapon Kayansamruaj, Tilladit Rung-ruangkijkrai, Saengchan Senapin, Channarong Rodkhum, Ha Thanh Dong
Summary: This study identified the distribution of TiLV in the entire brain of experimentally infected Nile tilapia, showing that the virus was broadly distributed throughout the forebrain and hindbrain. The virus may enter the brain through the circulatory system and induce brain dysfunction. Understanding the pattern of viral localization in the brain could help elucidate the neurological disorders of the diseased fish.
JOURNAL OF FISH DISEASES
(2021)
Article
Zoology
Nukul Saengphan, Bhinyo Panijpan, Saengchan Senapin, Parames Laosinchai, Auaree Suksomnit, Kornsunee Phiwsaiya
Summary: A new species of small freshwater prawn was discovered in the upper southern peninsula of Thailand. This new species, M. prachuapense sp. nov., has distinct characteristics that differentiate it from closely related species, and phylogenetic analysis confirms its unique status.
Article
Fisheries
Terawut Prasitporn, Saengchan Senapin, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: The study introduced a new method for detecting SDDV virus, utilizing UDG-assisted CPA combined with HNB and LFD technologies, which proved to be rapid, simple, and effective.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Thanwarat Sukonta, Saengchan Senapin, Watcharachai Meemetta, Thawatchai Chaijarasphong
Summary: A novel detection platform for SDDV based on CRISPR-Cas12a and recombinase polymerase amplification technology has been developed, showing high sensitivity and specificity for rapid and accurate diagnosis of SDDV. This user-friendly method can be completed within an hour at room temperature, potentially serving as a valuable tool for combating large-scale epidemics in fish farming communities.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Partho Pratim Debnath, Nguyen Dinh-Hung, Suwimon Taengphu, Vuong Viet Nguyen, Jerome Delamare-Deboutteville, Saengchan Senapin, Chadag Vishnumurthy Mohan, Ha Thanh Dong, Channarong Rodkhum
Summary: Sixteen countries have reported the presence of tilapia lake virus (TiLV), an emerging pathogen affecting tilapia. In Bangladesh, 20% of farms tested positive for TiLV in tilapia, while co-cultivated fish species and invertebrates did not show signs of TiLV infection. Experimental infection only showed clinical signs in Nile tilapia, suggesting that species in polycultures may not be susceptible to TiLV.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Ha Thanh Dong, Pattiya Sangpo, Le Thanh Dien, Thao Thu Mai, Nguyen Vu Linh, Jorge del-Pozo, Krishna R. Salin, Saengchan Senapin
Summary: CAIB in the pancreas of adult Nile tilapia infected with TiPV serves as a diagnostic feature, indicating exclusive replication of TiPV in pancreatic tissues, often accompanied by co-infection with Streptococcus agalactiae and secondary granulomas.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Piti Amparyup, Supakarn Sungkaew, Walaiporn Charoensapsri, Paveena Tapaneeyaworawong, Parichat Chumtong, Patchari Yocawibun, Prarthana Pantong, Ratree Wongpanya, Chanprapa Imjongjirak, Sorawit Powtongsook
Summary: In this study, we identified PUFA biosynthesis-related genes in A. royi and found that copepodid and adult stages are high in LC-PUFAs. The increased gene expression in the adult stages is correlated with the higher concentration of DHA and EPA.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Piti Amparyup, Supakarn Sungkaew, Walaiporn Charoensapsri, Parichat Chumtong, Patchari Yocawibun, Paveena Tapaneeyaworawong, Ratree Wongpanya, Chanprapa Imjongjirak
Summary: This study conducted genome sequencing and analysis of Apocyclops royi, a small aquatic crustacean. The researchers identified genes related to the shrimp Toll pathway and discovered a novel antimicrobial peptide. The expression analysis indicated the importance of these genes in the immune response of copepods. This knowledge can be applied to future functional studies and disease prevention in copepod aquaculture.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Thitiya Rattanadilog Na Phuket, Walaiporn Charoensapsri, Piti Amparyup, Chanprapa Imjongjirak
Summary: The crab proline-rich AMP SpPR-AMP1 has potent antimicrobial activity against both Gram-positive and Gram-negative bacteria. This study demonstrates that SpPR-AMP1 has therapeutic effects against AHPND infection caused by Vibrio campbellii VH-639 and can enhance the innate immune response in shrimp Litopenaeus vannamei. SpPR-AMP1 disrupts the bacterial cell membrane and promotes shrimp's resistance against V. campbellii VH-639 infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Fisheries
Ha Thanh Dong, Thawatchai Chaijarasphong, Andrew C. Barnes, Jerome Delamare-Deboutteville, Peiyu Alison Lee, Saengchan Senapin, Chadag Vishnumurthy Mohan, Kathy F. J. Tang, Sharon E. McGladdery, Melba G. Bondad-Reantaso
Summary: The article "Diagnostics for diseases of tilapia: From basic to molecular diagnosis" explores the importance of the three levels of diagnostics for diseases of tilapia, particularly the frequently overlooked basic procedures. The article also covers current and emerging molecular diagnostic technologies for tilapia pathogens, and discusses future developments for transferring these technologies to farmers and stakeholders for a sustainable aquatic food system transformation.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Ramida Phasunon, Suwimon Taengphu, Wattana Panphut, Satid Chatchaiphan, Ha Thanh Dong, Saengchan Senapin
Summary: A newly developed probe-based qPCR method for the detection of S. iniae was reported in this study. The method demonstrated high specificity and sensitivity, and can be applied for the diagnosis and surveillance of S. iniae infections in fish populations.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Nguyen Vu Linh, Nareerat Khongcharoen, Dinh-Hung Nguyen, Le Thanh Dien, Naruporn Rungrueng, Chayuda Jhunkeaw, Pattiya Sangpo, Saengchan Senapin, Toungporn Uttarotai, Wattana Panphut, Sophie St-Hilaire, Hien Van Doan, Ha Thanh Dong
Summary: This study investigated the effects of oxygen nanobubble treatment on Nile tilapia. The results showed that both short-term and long-term exposure to high oxygen levels did not have obvious adverse effects on the fish, although there were some changes in the gut microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2023)