Article
Chemistry, Analytical
Andrea Di Credico, Giulia Gaggi, Pascal Izzicupo, Laura Ferri, Laura Bonanni, Giovanni Iannetti, Angela Di Baldassarre, Barbara Ghinassi
Summary: This study utilized advanced technology to investigate the electrophysiological effects of Levetiracetam (LEV) on human neurons, showing significant effects on different variables, but neurons were able to completely recover their baseline activity within 24 hours.
Article
Biophysics
Danny Hsu Ko, David Bates, Hande Karaosmanoglu, Karl Taredun, Clare Elton, Leonie Jones, Ali Hosseini, Ashton Partridge
Summary: The increased sensitivity of microelectrode arrays (MEAs) over macroelectrodes for biosensing is due to the reduced diffusion gradient of the target species to and from the electrode surfaces. A polymer-based 3D MEA exhibits higher sensitivity by enhancing the diffusion profile of the target species and inducing differential current distribution focused at the apices of individual electrodes. The electrochemical characteristics of the 3D MEAs show ideal microelectrode behavior with three orders of magnitude greater sensitivity than ELISA.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Inorganic & Nuclear
Anees A. Ansari, Bansi D. Malhotra
Summary: The development of EC biosensors based on organic-inorganic hetero-nano-interfaces has made substantial progress in the detection of pathogenic diseases. These platforms offer long-term thermos-chemical stability, good linearity range, low detection limit, and high sensitivity. OIHNIs are suitable for integrating specific biological events with high-speed electrical signaling pathways, leading to the production of new bioelectronic devices. The research on OIHNIs-based EC biosensors summarizes the current state and discusses the use of transducers to detect a wide range of biological molecules and analytes with high sensitivity and selectivity.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Jin Song, Ashaq Ali, Yaohong Ma, Yiwei Li
Summary: In this study, a microfluidic system using a graphene-modified microelectrode array sensor was proposed for real-time monitoring of bacterial biofilms through electrochemical impedance. The results showed that increasing bacterial cell load on the sensing interface significantly prolonged the observation window period of the biofilm state, thereby improving the sensing signal quality. Simulation studies demonstrated that this method outperformed traditional endpoint detection in anti-biofilm drug screening.
NANOSCALE ADVANCES
(2023)
Review
Biochemistry & Molecular Biology
Yasir Qasim Almajidi, Jitendra Gupta, Fatime Satar Sheri, Rahman S. Zabibah, Ahmed Faisal, Akbarali Ruzibayev, Mohaned Adil, Mohamed J. Saadh, Mahmood Jasem Jawad, Fahad Alsaikhan, Asghar Narmani, Bagher Farhood
Summary: In this review, recent achievements and advances associated with chitosan-based hydrogels for drug delivery, regenerative medicine, disease detection, and therapy are summarized.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biophysics
Sung Sik Chu, Hung Anh Nguyen, Derrick Lin, Mehwish Bhatti, Carolyn E. Jones-Tinsley, An Hong Do, Ron D. Frostig, Zoran Nenadic, Xiangmin Xu, Miranda M. Lim, Hung Cao
Summary: Real-time tracking of neurotransmitter levels in vivo has been challenging due to the limitations of current methods. This study developed a flexible electrochemical microsensor for simultaneous detection of L-glutamate and GABA, crucial neurotransmitters associated with the balance of cortical excitation/inhibition ratios. The sensor was validated in vitro, ex vivo, and in vivo, and showed promise for accurate, real-time, and long-term monitoring of the E:I balance in neurological disorders.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemical Research Methods
Metka Stantic, Gregor Guncar, Drago Kuzman, Rok Mravljak, Tamara Cvijic, Ales Podgornik
Summary: An affinity column based on lectins was developed for monitoring glycosylated protein concentration in perfusion bioprocesses. The immobilized lectin showed improved binding kinetics towards Fc-fusion protein with the introduction of a spacer and complete desorption using 0.25 M galactose was achieved. The affinity column exhibited linearity in a specific concentration range and flow-unaffected binding for certain flow rates, along with long-term stability and no unspecific binding of culture media components.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Yuan Bai, Zongxian Jing, Rui Ma, Xinwen Wan, Jie Liu, Weiting Huang
Summary: Enzymes used in industrial processes have limitations such as instability, sensitivity, and difficult recovery. Immobilization technology using chitosan composites has attracted attention for improving enzyme applications. This review explores the effects of chitosan structure, molecular weight, degree of deacetylation on immobilized enzymes, key factors, and applications in biosensors, biomedical science, food industry, environmental protection, and industrial production. It also discusses the advantages, disadvantages, and future potential of using these composites.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Biochemical Research Methods
Mario Lederle, Mircea Tric, Tatjana Roth, Lina Schuette, Anke Rattenholl, Dirk Luetkemeyer, Stefan Woelfl, Tobias Werner, Philipp Wiedemann
Summary: This research focuses on improving the productivity, consistency, and quality of biopharmaceutical processes and products by developing new sensors for continuous monitoring of critical parameters. The glucose monitoring system developed in this study successfully maintains stable glucose levels over extended periods and does not have negative effects on protein quantity and quality.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Jingyu Xie, Yuchuan Dai, Yu Xing, Yiding Wang, Gucheng Yang, Enhui He, Zhaojie Xu, Penghui Fan, Fan Mo, Yirong Wu, Yilin Song, Xinxia Cai
Summary: Researchers developed a novel method using carboxylated graphene oxide (cGO) to couple glutamate oxidase (GluOx) biomolecules onto neural microelectrodes for sensitive monitoring of glutamate concentration and electrophysiology in epileptic rats. The resulting glutamate IMEA exhibited good performance with less signal crosstalk, lower reaction potential, and higher linear sensitivity.
Article
Biophysics
Amir Niaraki, Mehran Abbasi Shirsavar, Saurabh S. Aykar, Mehrnoosh Taghavimehr, Reza Montazami, Nicole N. Hashemi
Summary: Real-time and high-throughput monitoring of neural cells exposed to injury mechanisms using microelectrode arrays and electrical impedance spectroscopy is demonstrated in this study. Aqueous graphene is used for microelectrode fabrication, and the surface morphology and electrical properties of the electrodes are characterized. The attachment and impedance changes of the neural population on the graphene microelectrodes are quantified, and the impact of UV exposure on neural detachment is monitored.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Bo Tang, Mengxi Liu, Andreas Dietzel
Summary: Electrical impedance spectroscopy (EIS) is a powerful tool in biomedical research, but single-channel systems lack spatial resolution. Researchers have developed a low-cost multichannel setup using a microelectrode array (MEA) to map cell distributions in a fluidic environment. The MEA, connected to programmable multiplexers and an analog front-end module, produced impedance maps that matched optical images of yeast cell distribution in a reservoir.
Article
Chemistry, Analytical
Thanih Balbaied, Eric Moore
Summary: The study proposed a method using biosensor-based strategies for real-time monitoring of ALP release from human colon cancer cells, facilitating understanding of cancer progression and emerging diseases. Results showed a correlation between cell response and changes in impedance magnitude and current, influencing cell morphology and viability. The developed method represents an advance over current techniques and may contribute to microfluidic testing development for small-scale techniques.
ANALYTICAL LETTERS
(2022)
Article
Biophysics
Xiaoting Sun, Yuting Xiang, Min Liu, Xinyu Xu, Liping Zhang, Liujing Zhuang, Ping Wang, Qin Wang
Summary: In this study, a portable high-throughput cardiomyocyte-based potential biosensor was established for the rapid and quantitative detection of ion channel toxins. The biosensor showed high sensitivity and specificity, and could detect the toxins within a short time frame of 5 to 15 minutes.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Materials Science, Biomaterials
Pranay Ranjan, Vinoy Thomas, Prashant Kumar
Summary: The COVID-19 pandemic has significantly impacted global economies and public health, posing immense challenges for many countries. Emerging 2D materials like graphene provide an effective platform for the diagnosis and treatment of SARS-CoV-2, offering promising solutions for the current crisis.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)