4.6 Article

Sono-assisted enzymatic saccharification of sugarcane bagasse for bioethanol production

期刊

BIOCHEMICAL ENGINEERING JOURNAL
卷 63, 期 -, 页码 1-9

出版社

ELSEVIER
DOI: 10.1016/j.bej.2012.01.001

关键词

Ethanol; Bioconversion; Cellulase; Sono-assisted saccharification; Fermentation

资金

  1. DST, New Delhi

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This study presents the sono-assisted pretreatment and enzymatic saccharification of sugarcane bagasse (SCB) for the production of bioethanol. The effect of sono-assisted alkali (NaOH) pretreatment on the removal of hemicellulose and lignin from SCB was studied and the results showed 80.8% of hemicellulose and 90.6% of lignin removal. Sono-assisted enzymatic saccharification was performed with Cellulomonas flavigena (MTCC 7450) and the yield was found to be affected by liquid-to-solid ratio (LSR), cell mass and pH. The optimum reaction time, LSR, cell mass and pH were found to be 360 min, 15:1, 15 g/L and 6.0 respectively. At optimum conditions, the maximum glucose yield obtained was 91.28% of the theoretical yield and the maximum amount of glucose obtained was 38.4 g/L. The enhancement in performance may be correlated with the swelling of cellulose and accelerated enzymatic saccharification due to the application of ultrasound. The hydrolyzate obtained was fermented using Zymomonas mobilis (MTCC 89) and about 91.22% of the theoretical ethanol yield was observed in 36 h of fermentation. (C) 2012 Elsevier B.V. All rights reserved.

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