Article
Biochemistry & Molecular Biology
Jiali Li, Bohao Zhao, Shuyu Yao, Yingying Dai, Xiyu Zhang, Naisu Yang, Zhiyuan Bao, Jiawei Cai, Yang Chen, Xinsheng Wu
Summary: This study found that exosomes derived from dermal papilla cells regulate hair follicle stem cell proliferation by stimulating LEF1, providing new insights into the regulatory mechanisms of hair follicle growth and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Ramya Lakshmi Rajendran, Prakash Gangadaran, Mi Hee Kwack, Ji Min Oh, Chae Moon Hong, Young Kwan Sung, Jaetae Lee, Byeong-Cheol Ahn
Summary: Research has found that extracellular vesicles derived from human mesenchymal stem cells have positive effects on hair growth. These vesicles promote the proliferation and migration of dermal papilla and outer root sheath cells, as well as enhance the expression of keratin markers, ultimately leading to hair shaft elongation.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Cell Biology
Masahiro Fukuyama, Aki Tsukashima, Momoko Kimishima, Yoshimi Yamazaki, Hideyuki Okano, Manabu Ohyama
Summary: This study attempted to reproduce the anatomical relationship between the epithelial main body and the dermal papilla within hair follicles in vitro and evaluated how follicle characteristics were reproduced in constructs. Using hiPSC-derived cell composites, the study showed promising potential for regenerating hair follicle equivalents with in vitro hair inductive capacity, reducing the reliance on human tissue-derived trichogenic cell subsets.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Dermatology
Pieter A. Martino, Nicholas Heitman, Michael Rendl
Summary: Hair follicles cyclically regenerate in adult mammals due to stem cell activity. The dermal papilla plays a crucial role in regulating stem cell activation and progenitor proliferation and differentiation during follicle growth.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jae Young Shin, Jaeyoon Kim, Yun-Ho Choi, Nae-Gyu Kang, Sanghwa Lee
Summary: The study revealed that D-panthenol can enhance the viability of hair follicle cells, suppress apoptotic and senescence markers, and promote the elongation of the anagen phase in hair follicles.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hanluo Li, Federica Francesca Masieri, Marie Schneider, Alexander Bartella, Sebastian Gaus, Sebastian Hahnel, Ruediger Zimmerer, Ulrich Sack, Danijela Maksimovic-Ivanic, Sanja Mijatovic, Jan-Christoph Simon, Bernd Lethaus, Vuk Savkovic
Summary: The mid part of the hair follicle outer root sheath (ORS) contains a rich population of stem cells with the potential to give rise to multiple stem cell types in vitro. Comparative analysis shows that the mid-ORS part exhibits higher expression levels of key markers at both the protein and gene levels when compared to the distal ORS part.
Article
Medicine, General & Internal
Vuk Savkovic, Hanluo Li, Danilo Obradovic, Federica Francesca Masieri, Alexander K. Bartella, Rudiger Zimmerer, Jan-Christoph Simon, Christian Etz, Bernd Lethaus
Summary: Neovascularization is essential for successful tissue grafting, and mesenchymal stem cells from hair follicle outer root sheath (MSCORS) exhibit strong angiogenic potential for future applications in tissue engineering. Compared to adipose mesenchymal stem cells, MSCORS show higher differentiation efficiency and greater angiogenic potential in endothelial derivatives.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Dermatology
Fang Liu, Si Liu, Xiaohua Luo, Zirui Fan, Shaobin Huang, Fangqi Deng, Huanliang Liu, Ge Shi
Summary: The study demonstrates that exosomes derived from DSCCs have an anti-ageing effect on DPCs, promoting their proliferation, viability, and migration while inhibiting apoptosis. The exosomes also enhance hair follicle induction ability and activate relevant signaling pathways, potentially serving as a new strategy for preventing senile alopecia.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu-Jin Lee, Song-Hee Park, Hye-Ree Park, Young Lee, Hoon Kang, Jung-Eun Kim
Summary: This study demonstrated that hHMSCs promoted the viability and migration of hORSCs and investigated the mechanisms associated with the Wnt/β-catenin and JAK/STAT pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Chemical
Hanluo Li, Jule Kristin Michler, Alexander Bartella, Anna Katharina Sander, Sebastian Gaus, Sebastian Hahnel, Ruediger Zimmerer, Jan-Christoph Simon, Vuk Savkovic, Bernd Lethaus
Summary: This study successfully translated the technology of human outer root sheath (ORS) to horses, resulting in the culture of equine melanocytes from hair follicles. The procedure was adjusted to fit equine physiology, providing an experimental base for further understanding of hair follicle biology in horses.
Article
Biochemistry & Molecular Biology
Qing Wang, Mei Zhou, Hongyan Zhang, Zhuang Hou, Dongjun Liu
Summary: Dermal papilla cells (DPCs) cultured in a hypoxic microenvironment can induce hair follicle formation. Culturing adipose mesenchymal stem cells (ADSCs) in a hypoxic microenvironment promotes hair follicle growth. This study used ADSCs and DPCs from Arbas Cashmere goats to investigate the molecular mechanisms involved. The hypoxic microenvironment enhanced the proliferation and pluripotency of ADSCs, and upregulated the expression of growth factors in ADSCs. It also improved the ability of DPCs to induce hair follicle formation. These findings provide a theoretical basis for the development of gene therapy drugs for the treatment of alopecia areata and hair thinning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Jong-Hyuk Sung
Summary: This article reviewed and summarized the latest research reports on the characteristics of stem cells and follicular cells for hair loss treatment. It found that cell therapy could be more effective and have milder side effects compared to conventional medicine. Adipose-derived stem cells (ASCs) have advantages in accessibility and quantity compared to dermal papilla cells (DPCs) and dermal sheath cup cells (DSCs), while DSCs have immune tolerance and hair inductivity. Although DPCs have not been studied in clinical trials, they have the potential to create new hair follicles and enable allogeneic transplantation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Carla M. Abreu, Mariana T. Cerqueira, Rogerio P. Pirraco, Luca Gasperini, Rui L. Reis, Alexandra P. Marques
Summary: The conditioned medium from interfollicular keratinocytes (KCs-CM) has been shown to improve the hair inductive ability of DP cells, with significant effects on DP cell growth and restoration of activity. Additionally, DP cells cultured with KCs-CM in spheroids demonstrated superior activity and supported the formation of hair follicle- and sebaceous gland-like structures in mice when co-grafted with human keratinocytes.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hanluo Li, Hafiz Awais Nawaz, Federica Francesca Masieri, Sarah Vogel, Ute Hempel, Alexander K. Bartella, Rudiger Zimmerer, Jan-Christoph Simon, Michaela Schulz-Siegmund, Michael Hacker, Bernd Lethaus, Vuk Savkovic
Summary: Autologous mesenchymal stem cells derived from the hair follicle outer root sheath (MSCORS) showed a remarkable capacity for differentiation towards the osteogenic lineage. When combined with a novel gelatin-based hydrogel called Osteogel, these cells provided additional osteoinductive cues, indicating a potential new approach in bone regeneration therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Mayumi Ikeda, Shigeharu Yabe, Masahiro Kiso, Naoko Ishiguro, Yuichiro Tsunemi, Hitoshi Okochi
Summary: This study evaluated the function of TERT and BMI1 in human DPCs. The results showed that overexpression of both TERT and BMI1 extends the lifespan of human DPCs and improves their hair inducing ability on mouse hair follicles.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)