Article
Chemistry, Medicinal
Anirban Sardar, Alisha Ansari, Sampa Gupta, Shradha Sinha, Shubham Pandey, Divya Rai, Mukesh Kumar, Rabi Sankar Bhatta, Ritu Trivedi, Koneni Sashidhara
Summary: A series of compounds were designed and synthesized using molecular hybridization approach. Among them, compound 47 was identified as the most potent osteoanabolic agent, promoting osteogenesis via the non-canonical p38 pathway mediated by RUNX2 and BMP2. It has the potential to be used as a lead for future bone metabolic disorder treatment.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cell & Tissue Engineering
Sanja Novak, Josip Madunic, Laura Shum, Milan Vucetic, Xi Wang, Hitoshi Tanigawa, Mallika Ghosh, Archana Sanjay, Ivo Kalajzic
Summary: Bone regeneration relies on a group of bone/cartilage stem/progenitor cells and their differentiation is regulated by signaling mechanisms. PDGF signaling through PDGFR beta was found to inhibit BMP2-induced osteogenesis and attenuate expression of BMP2 target genes. The outcomes of treatment with two anabolic agents, PDGF and BMP2, were evaluated in different bone healing models. In vivo study showed that PDGF inhibits BMP2-induced osteogenesis, but inhibiting PDGF signaling early in the healing process does not improve BMP2-induced bone healing.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Ruoxi Wang, Yu Wang, Yuting Niu, Danqing He, Shanshan Jin, Zixin Li, Lisha Zhu, Liyuan Chen, Xiaolan Wu, Chengye Ding, Tianhao Wu, Xinmeng Shi, He Zhang, Chang Li, Xin Wang, Zhengwei Xie, Weiran Li, Yan Liu
Summary: This study identified that dihydroartemisinin (DHA) can maintain the stemness of bone marrow mesenchymal stem cells (BMMSCs) and enhance bone regeneration. DHA preserves the stemness characteristics of BMMSCs during in vitro expansion and restores bone structure and BMMSC stemness in an osteoporosis mouse model. Mechanistically, DHA maintains BMMSC stemness by promoting histone acetylation and can be delivered to bones using mesoporous silica nanoparticles to improve therapeutic efficacy in osteoporosis.
ACS CENTRAL SCIENCE
(2023)
Article
Cell Biology
Yi Wang, Ying Gan, Yuan Dong, Jie Zhou, Endong Zhu, Hairui Yuan, Xiaoxia Li, Baoli Wang
Summary: Tax1bp3 is a PDZ domain-containing protein that is overexpressed in cancer and can regulate the differentiation of mesenchymal progenitor cells into osteoblasts and adipocytes. It inhibits osteogenic differentiation and promotes adipogenic differentiation by inhibiting the activation of Wnt/beta-catenin and BMPs/Smads signaling pathways.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Genetics & Heredity
Yuanxin Miao, Yunxia Zhao, Siqi S. Wan, Quanshun Mei, Heng S. Wang, Chuanke Fu, Xinyun S. Li, Shuhong Zhao, Xuewen S. Xu, Tao Xiang
Summary: This study identified candidate genes and mutations affecting the loin muscle depth (LMD) in pigs using genome-wide association study (GWAS) and 3D epigenomics analysis. The BMP2 gene and SNPs rs321846600 and rs1111440035 were found to be associated with LMD. The integration of GWAS and 3D epigenomics provides valuable insights into the identification of candidate genes for quantitative traits in pigs.
Article
Medicine, Research & Experimental
Jue Hu, Zhuozhi Wang, Jacob M. Miszuk, Erliang Zeng, Hongli Sun
Summary: FDA-approved bone morphogenetic protein 2 (BMP2) has serious side effects, but poly(glutamic acid) (pGlu) shows potential to improve BMP2 bioactivity with less safety concerns. Both low and high molecular weight pGlu can significantly enhance BMP2-induced osteoblastic differentiation and matrix mineralization, with the high molecular weight pGlu being more effective. Alpha-H-pGlu enables a single dose of BMP2 to induce high levels of osteoblastic differentiation without multiple doses. Additionally, H-pGlu can be incorporated into collagen hydrogel or coated on 3D porous gelatin and chitosan scaffolds to enhance osteogenic differentiation through sustained release of BMP2.
MOLECULAR PHARMACEUTICS
(2022)
Article
Endocrinology & Metabolism
Ariane Zamarioli, Gremah Adam, Kevin A. A. Maupin, Paul J. J. Childress, Alexander Brinker, Joao P. B. Ximenez, Nabarun Chakraborty, Aarti Gautam, Rasha Hammamieh, Melissa A. A. Kacena
Summary: The study found that administering BMP2 at the fracture site of mice had systemic effects on distant bones and the mechanisms of action may differ between Earth and spaceflight.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, General & Internal
Jing-Xiao Li, Jin-Shu Pang, Bin-Tong Yin, Gang Chen, Jun-Hong Chen, Jia-Yuan Luo, Xia Yang, Li-Ting Qin, Jiang-Hui Zeng, Peng Chen, Jia-Bo Chen, Deng Tang
Summary: The study found that ATF3 is downregulated in hepatoblastoma, possibly playing a significant role in disease occurrence by regulating mechanisms like ferroptosis.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Wilson Cheuk Wing Chan, Zhijia Tan, Michael Kai Tsun To, Danny Chan
Summary: Bone is a dynamic tissue that responds to environmental changes such as nutrition and mechanical stress. Bone homeostasis is maintained through bone remodeling in adulthood, which involves a controlled balance between bone-resorbing osteoclasts and bone-forming osteoblasts. During embryonic development, bones can form through intramembraneous or endochondral ossification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Nissrine Ballout, Agnes Boullier, Walaa Darwiche, Katia Ait-Mohand, Eric Trecherel, Theo Gallego, Cathy Gomila, Linda Yaker, Isabelle Gennero, Jose Kovensky, Jerome Ausseil, Sylvestre Toumieux
Summary: Bone fracture healing is a complex process involving hematoma formation, tissue formation, bony callus formation, and bone remodeling. Current therapeutic strategies for fracture repair include autografts, allografts, and tissue engineering. This study suggests that DP2, a disaccharide compound, may enhance fracture healing by promoting osteoblast differentiation and earlier mineralization in vitro. DP2 might serve as a potential therapeutic candidate for bone fracture repair.
Article
Biotechnology & Applied Microbiology
Rongkui Zhou, Shichang Miao, Jun Xu, Liping Sun, Yaofei Chen
Summary: The study showed that Circ_0000020 is upregulated during osteogenic differentiation and interacts with miR-142-5p to regulate BMP2 expression, promoting osteogenic differentiation of BMSCs. This positive regulation occurs through the Circ_0000020/miR-142-5p/BMP2/SMAD-dependent signaling pathway.
Article
Nutrition & Dietetics
Yu Jin Hwang, Hye-Jeong Hwang, Hyunseo Go, Nayeong Park, Kyung-A Hwang
Summary: Sword bean and sword bean pod extracts can promote osteoblast differentiation and increase osteoblast proliferation and activity.
Article
Multidisciplinary Sciences
Paul D. Benya, Aaron Kavanaugh, Martin Zakarian, Philip Soderlind, Tea Jashashvili, Nianli Zhang, Erik Waldorff, James T. Ryaby, Fabrizio Billi
Summary: The study shows that PEMF can stimulate osteogenesis at different times, potentially enhancing bone repair in a strong osteogenic environment.
Article
Chemistry, Medicinal
Seung-Hong Lee, Mihyang Kim, Mi Hwa Park
Summary: The study demonstrated that DPHC from Ishige okamurae has protective effects against oxidative damage in osteoblastic cells induced by H2O2 through the BMP2/Runx2 signaling pathway, improving cellular functions and promoting osteoblast differentiation. DPHC also reduced the expression levels of bone resorption-promoting factors and inhibited the generation of reactive oxygen species induced by oxidative stress.
Review
Endocrinology & Metabolism
Yan Li, Qiuyue Fan, Fengshi Li, Rui Pang, Chen Chen, Peiying Li, Xin Wang, Wei Xuan, Weifeng Yu
Summary: ATF3 is a crucial transcription factor that plays dual roles in inflammatory responses. It has been found to be involved in the neuroinflammatory response to acute brain injury (ABI) and may serve as a potential biomarker for the long-term prognosis of ABI patients.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Kanako Kobayashi, Tomohiro Tanaka, Sadanori Okada, Yuki Morimoto, Shigenobu Matsumura, Mark Christian C. Manio, Kazuo Inoue, Kumi Kimura, Takashi Yagi, Yoshihiko Saito, Tohru Fushiki, Hiroshi Inoue, Michihiro Matsumoto, Yo-ichi Nabeshima
Article
Nutrition & Dietetics
Hitoshi Watanabe, Yuka Inaba, Kumi Kimura, Shun-ichiro Asahara, Yoshiaki Kido, Michihiro Matsumoto, Takayasu Motoyama, Nobuhiko Tachibana, Shuichi Kaneko, Mitsutaka Kohno, Hiroshi Inoue
JOURNAL OF NUTRITION
(2017)
Article
Biochemical Research Methods
Dae-Jin Kwon, Dong-Hwan Kim, In-Sul Hwang, Dong-Ern Kim, Hyung-Joo Kim, Jang-Seong Kim, Kichoon Lee, Gi-Sun Im, Jeong-Woong Lee, Seongsoo Hwang
TRANSGENIC RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Akiho Nakatani, Xuan Li, Junki Miyamoto, Miki Igarashi, Hitoshi Watanabe, Asuka Sutou, Keita Watanabe, Takayasu Motoyama, Nobuhiko Tachibana, Mitsutaka Kohno, Hiroshi Inoue, Ikuo Kimura
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Hitoshi Watanabe, Yuka Inaba, Kumi Kimura, Michihiro Matsumoto, Shuichi Kaneko, Masato Kasuga, Hiroshi Inoue
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Keita Watanabe, Miki Igarashi, Xuan Li, Akiho Nakatani, Junki Miyamoto, Yuka Inaba, Asuka Sutou, Tsutomu Saito, Takumi Sato, Nobuhiko Tachibana, Hiroshi Inoue, Ikuo Kimura
Article
Endocrinology & Metabolism
Kumi Kimura, Yuka Inaba, Hitoshi Watanabe, Toshiya Matsukawa, Michihiro Matsumoto, Hiroshi Inoue
JOURNAL OF DIABETES INVESTIGATION
(2019)
Article
Endocrinology & Metabolism
Emi Hashiuchi, Hitoshi Watanabe, Kumi Kimura, Michihiro Matsumoto, Hiroshi Inoue, Yuka Inaba
Summary: SGLT2 inhibitors affect glucose and energy metabolism under controlled feeding conditions, but their effects are diminished under free feeding conditions. Food intake management is crucial for SGLT2 inhibitors to impact glucose and energy metabolism during type 2 diabetes treatment.
JOURNAL OF DIABETES INVESTIGATION
(2021)
Article
Biotechnology & Applied Microbiology
Dong-Ern Kim, Ji-Hye Lee, Kuk-Bin Ji, Kang-Sun Park, Tae-Young Kil, Okjae Koo, Min-Kyu Kim
Summary: Using SCNT combined with CRISPR-Cas9, genome-edited dogs were successfully generated with mutations at the target locus and altered expression of DJ-1.
Article
Biochemistry & Molecular Biology
Ahyoung Park, Hyun Ju Oh, Kukbin Ji, Eunha Miri Choi, Dongern Kim, Eunyoung Kim, Min Kyu Kim
Summary: This study demonstrates the positive effects of equine amniotic fluid mesenchymal stem cell conditioned medium (eAFMSC-CMp7) on oocyte and embryonic development. eAFMSC-CMp7 improves cytoplasmic maturation, enhances blastocyst quality, and has antioxidant effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Dong Ern Kim, Ji Hye Lee, Kuk Bin Ji, Eun Ji Lee, Chuang Li, Hyun Ju Oh, Kang Sun Park, Seung Hoon Lee, Okjae Koo, Min Kyu Kim
Summary: Canine hip dysplasia (HD) is a multifactorial disease caused by interactions between genetic and environmental factors, mainly occurring in medium- to large-sized dogs; excluding individual dogs with HD from breeding programs is currently the most effective way to improve the situation; the study suggests that prime editing (PE) technology can potentially be used as a platform to correct genetic defects in dogs.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Yuka Inaba, Emi Hashiuchi, Hitoshi Watanabe, Kumi Kimura, Yu Oshima, Kohsuke Tsuchiya, Shin Murai, Chiaki Takahashi, Michihiro Matsumoto, Shigetaka Kitajima, Yasuhiko Yamamoto, Masao Honda, Shun-ichiro Asahara, Kim Ravnskjaer, Shin-ichi Horike, Shuichi Kaneko, Masato Kasuga, Hiroyasu Nakano, Kenichi Harada, Hiroshi Inoue
Summary: Aggravation of liver steatosis shifts the mode of hepatocellular death from apoptosis to necroptosis, and the transcription factor ATF3 regulates this shift through the induction of RIPK3. ATF3 acts as a master regulator in this shift by inducing RIPK3 expression. The frequency of hepatocytes expressing ATF3 or RIPK3 is correlated with hepatocellular damage in non-alcoholic steatohepatitis (NASH).
NATURE COMMUNICATIONS
(2023)
Article
Endocrinology & Metabolism
Yuka Inaba, Emi Hashiuchi, Hitoshi Watanabe, Kumi Kimura, Makoto Sato, Masaki Kobayashi, Michihiro Matsumoto, Tadahiro Kitamura, Masato Kasuga, Hiroshi Inoue
Article
Endocrinology & Metabolism
Yasuhiro Onogi, Tsutomu Wada, Chie Kamiya, Kento Inata, Takatoshi Matsuzawa, Yuka Inaba, Kumi Kimura, Hiroshi Inoue, Seiji Yamamoto, Yoko Ishii, Daisuke Koya, Hiroshi Tsuneki, Masakiyo Sasahara, Toshiyasu Sasaoka
Review
Endocrinology & Metabolism
Hiroshi Inoue
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)