Article
Cell Biology
Petr Fojtik, Deborah Beckerova, Katerina Holomkova, Martin Senfluk, Vladimir Rotrekl
Summary: Mild hypoxia attenuates the pluripotency-promoting PI3K/AKT signaling pathway in human pluripotent stem cells compared to normoxic conditions, due to the downregulation of ROS. In addition, the MAPK pathway also downregulates ROS and attenuates the PI3K/AKT signaling via a novel interaction between these pathways. This ROS-mediated regulatory circuit may have implications for enhancing cultivation and differentiation protocols for hPSCs, as well as providing insights into cancer stem cell signaling regulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Chemical
Tae-Won Jang, Jae-Ho Park
Summary: The research demonstrated that the callus of Abeliophyllum distichum has anti-inflammatory effects by inhibiting the MAPK, PI3K/Akt, and NF-kappa B signaling pathways. The presence of acteoside and isoacteoside in CAO contributes to its potential as an anti-inflammatory agent by suppressing the release of inflammation factors in RAW 264.7 cells.
Article
Peripheral Vascular Disease
Mohammad Shafid, Kumaravelu Jagavelu, Hina Iqbal, Pankaj Yadav, Debabrata Chanda, Neeraj Kumar Verma, Jimut Kanti Ghosh, Matthias Gaestel, Kashif Hanif
Summary: MK2 plays a significant role in pulmonary hypertension, and its inhibition may be a novel target for PH treatment. Inhibition of MK2 alleviated right ventricle pressure and hypertrophy in PH rats, improved cardiac function, reversed pulmonary vascular remodeling, and enhanced pulmonary vascular relaxation.
Article
Peripheral Vascular Disease
Yuqing Shi, Jie Liu, Ruoyang Zhang, Muzhi Zhang, Han Cui, Lei Wang, Ye Cui, Wei Wang, Ying Sun, Chen Wang
Summary: It has been shown that glycolytic protein ENO1 contributes to the pathogenesis of pulmonary hypertension by acting on smooth muscle cells. However, the specific roles of ENO1 in endothelial and mitochondrial dysfunctions in Group 3 pulmonary hypertension have not been explored. This study revealed that hypoxic pulmonary hypertension is associated with increased ENO1 expression and targeting ENO1 can reduce hypoxic pulmonary hypertension by improving endothelial and mitochondrial dysfunction via the PI3K-Akt-mTOR signaling pathway.
Article
Oncology
Wenli Chen, Guangli Dai, Yike Qian, Lian Wen, Xueqing He, Hui Liu, Yunxing Gao, Xingli Tang, Bohan Dong
Summary: The PIK3CA mutation in colorectal cancer (CRC) renders the cells more dependent on glutamine and promotes cell proliferation and resistance to suboptimal environments. The mutated PIK3CA regulates GPT2 expression through the MEK/PDK1 dual pathway. Targeting this pathway can effectively inhibit CRC cell proliferation.
Article
Oncology
Angela Duff, Llona Kavege, Jocelyn Baquier, Tang Hu
Summary: LY294002, initially reported as a selective inhibitor of PI3K-Akt, was found to inhibit other molecules as well. The study showed that LY294002 significantly inhibits proliferation and induces cluster formation in MV4-11, TF-1a, and Hep-G2 cells. Furthermore, LY294002 repressed the activation of MEK and ERK signal molecules in all cells, suggesting a potential direct inhibition mechanism.
Review
Cell Biology
Shen-Han Lee, Monika Golinska, John R. Griffiths
Summary: Cancer cells in hypoxic microenvironments can adapt metabolically to HIF-1 inhibition through various mechanisms, potentially leading to drug resistance. Future research should investigate these pathways to uncover hypoxia bypass mechanisms in anti-HIF-1 cancer therapy. Agents targeting HIF-1 beta may be advantageous, but off-target effects and potential resistance mechanisms need careful consideration in cancer therapy by HIF inhibition.
Article
Biochemistry & Molecular Biology
Hsin-Yu Ho, Ping-Ju Chen, Yi-Ching Chuang, Yu-Sheng Lo, Chia-Chieh Lin, Ming-Ju Hsieh, Mu-Kuan Chen
Summary: Picrasidine I exerts anticancer effects in nasopharyngeal carcinoma by modulating HO-1 via the ERK and Akt signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Qian Wang, Chenqi Tao, Abdul Hannan, Sungtae Yoon, Xuanyu Min, John Peregrin, Xiuxia Qu, Hongge Li, Honglian Yu, Jean Zhao, Xin Zhang
Summary: The patterning of epithelial buds in lacrimal gland development is determined by the interplay between PI3K, Ras, and ERK signaling pathways. PI3K is activated by both IGF and FGF signaling, and also promotes ERK signaling through direct interaction with Ras. Inhibition of EGF signaling is essential for induction of lacrimal gland buds, and the cross-talk among FGF, IGF, and EGF signaling is mediated by PI3K, MAPK, and mTOR.
Article
Oncology
Kreon Koukoulas, Antonis Giakountis, Angeliki Karagiota, Martina Samiotaki, George Panayotou, George Simos, Ilias Mylonis
Summary: ERK-mediated phosphorylation of HIF-1 alpha enhances its interaction with NPM1, which influences the transcriptional activation of HIF-1 target genes. NPM1 and HIF-1 co-regulate genes enriched in different cancer types, and their expression correlates with hypoxic tumor status and patient prognosis.
MOLECULAR ONCOLOGY
(2021)
Article
Oncology
Evan K. Noch, Laura N. Palma, Isaiah Yim, Nayah Bullen, Yuqing Qiu, Hiranmayi Ravichandran, Junbum Kim, Andre Rendeiro, Melissa B. Davis, Olivier Elemento, David J. Pisapia, Kevin Zhai, Hongbiao Carl LeKaye, Jason A. Koutcher, Patrick Y. Wen, Keith L. Ligon, Lewis C. Cantley
Summary: This study investigated the critical role of insulin feedback in the poor clinical efficacy of phosphatidylinositol 3-kinase (PI3K) inhibition in cancer and the independent association between hyperglycemia and poor prognosis in glioblastoma (GBM). It was found that combining anti-hyperglycemic therapy with PI3K inhibition improved treatment efficacy in a GBM mouse model. Analysis of clinical trial data revealed that hyperglycemia was independently associated with poor progression-free survival in GBM patients.
Article
Multidisciplinary Sciences
Mengdong Wang, Di Wang, Yue Lang, Anwen Shao, Rui Zhang, Jun Tang, Dongming Lai, Chenglu Xiao
Summary: HIF-1α is crucial for hypoxia response by upregulating gene expression. L3MBTL3, regulated by HIF-1α, interacts with and promotes the degradation of HIF-1α in the nucleus, forming a negative feedback loop to dampen the hypoxic response.
Article
Respiratory System
Ming Li, Huan Liu, Juan Li, Shuai Guo, Yan Lv
Summary: This study reveals that CKMT1 is highly expressed in non-small cell lung cancer and is correlated with pathological grade. Knockdown of CKMT1 inhibits cell proliferation and invasion, but this effect can be rescued under hypoxic conditions. HIF-1 plays a role in up-regulating CKMT1 expression under hypoxia.
RESPIRATORY RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Hong-Beom Park, Kwang-Hyun Baek
Summary: This review summarizes the role and mechanisms of MAPK signaling pathways in cancer, as well as the functions of E3 ligases and DUBs targeting MAPK signaling components.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Review
Cell Biology
Nasim Kheshtchin, Jamshid Hadjati
Summary: Hypoxia, a common characteristic of solid tumors, contributes to different aspects of tumor progression and limits the efficacy of immunotherapies. Developing new immunotherapy strategies involving therapeutic targeting of HIF-1 molecules associated with hypoxia may enhance the clinical effectiveness of immunotherapy. Targeting hypoxia presents a potential opportunity to improve the clinical benefit of cancer immunotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)