Article
Veterinary Sciences
Libin Chen, Jiayu Ruan, Yiyi Chen, Wenxuan Deng, Jinyu Lai, Lei Fan, Juncheng Cai, Chan Ding, Qiuyan Lin, Bin Xiang, Tao Ren
Summary: In this study, circRNA transcriptome sequencing was used to analyze the differences in circRNA expression profiles after velogenic NDV infection in chicken embryo fibroblasts (CEFs). The results showed that NDV infection altered circRNA expression profiles in CEFs and revealed significant enrichment of differentially expressed (DE) circRNAs for metabolism-related pathways. Further analysis and validation demonstrated that circRNAs are involved in NDV replication by regulating metabolism-related genes and miRNA. These findings provide new insights into the mechanisms underlying NDV-host interactions.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Lei Fan, Jinlian Ren, Yinchu Wang, Yiyi Chen, Yichun Chen, Libin Chen, Qiuyan Lin, Ming Liao, Chan Ding, Bin Xiang, Tao Ren
Summary: This study reveals that DEF cells exert antiviral responses to NDV infection by forming circRNAs, which regulate pathways related to apoptosis, cell growth, and protein degradation. The circRNA-miRNA-mRNA interaction networks demonstrate that DEF cells combat NDV infection by regulating cellular pathways or apoptosis through circRNA-targeted mRNAs and miRNAs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Sebastian Gnat, Dominik Lagowski, Aneta Nowakiewicz, Aleksandra Troscianczyk, Mariusz Dylag
Summary: This study examined the suitability of six candidate reference genes for gene expression analysis in the dermatophyte Trichophyton verrucosum cultured in different mycological media. SDHA was identified as the most stable housekeeping gene for future qRT-PCR studies on T. verrucosum strains, suggesting it as an additional good candidate for identification targets in the qRT-PCR technique.
Article
Microbiology
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu
Summary: This study tested the stability of ten classic housekeeping genes under the pressure of eight commonly used antibiotics in the laboratory. Results showed that the stability of gene expression was indeed affected by the types of antibiotics added, and the best reference gene set varied for different antibiotics. This study provides a comprehensive summary of the effects of laboratory antibiotics on the stability of housekeeping genes in P. aeruginosa, highlighting the necessity to select housekeeping genes according to the type of antibiotics used in the initial stage of experiment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Nirmal Kumar Sampathkumar, Venkat Krishnan SundaramID, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aida Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
Summary: The choice of reference genes heavily influences the assessment of differential gene expression by qPCR. Recent studies suggest using RNA-Seq to identify stable genes and statistical approaches to determine the best reference genes. However, this study demonstrates that the statistical approach to determine the best reference genes from commonly used candidates is more important than preselecting stable genes from RNA-Seq data.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Agronomy
Junliang Yin, Lu Hou, Xinchen Jiang, Jie Yang, Yang He, Xiaokang Zhou, Xiongmeng Zhu, Andong Gong, Yongxing Zhu, Zhongyi Chen
Summary: Alligatorweed is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance. This study identified stable reference genes for gene expression analysis in A. philoxeroides, laying a foundation for further research on gene expression profiling and invasive weed management.
Article
Agriculture, Dairy & Animal Science
Ling Wang, Zhen Xue, Jinping Wang, Yuwen Jian, Hongzhao Lu, Haidong Ma, Shanshan Wang, Wenxian Zeng, Tao Zhang
Summary: Newcastle disease virus (NDV) is an RNA virus that infects poultry, causing serious damage to their health. The role of chicken Mx in antivirus invasion is still controversial, but this study found that its deficiency resulted in higher virus titers and exacerbated cell pathological features post-NDV infection. The absence of Mx also led to increased expression levels of IRF-7, IFN-a, IFN-f3, and pro-inflammatory cytokine TNF-a, but it alleviated the harm from virus infection by enhancing the expression of p21, p27, and Bak, which are related to cell proliferation and apoptosis.
Article
Infectious Diseases
Haijin Liu, Jianxia Tian, Kejia Lu, Yangyang Li, Zhao Guan, Xuhong Cao, Xiaoqin Li, Zhengwu Chang, Xinglong Wang, Xiao Sa, Zengqi Yang
Summary: This research identified chicken interferon-stimulated gene 12-2 as a potential adjuvant that can attenuate the virulence of Newcastle disease virus and increase the immunogenicity of the vaccine strain. It may contribute to the development of efficient poultry vaccines.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Plant Sciences
Chuang Zhan, Yiting Li, Han Li, Mengru Wang, Shuangjun Gong, Dongfang Ma, Yan Li
Summary: This study identified 54 PAL genes in the wheat genome and analyzed their expression levels under biotic stresses. Through further analysis and experimentation, it was confirmed that two of these genes, TaPAL32 and TaPAL42, play a positive role in wheat resistance to stripe rust. This research provides potential application value for breeding disease-resistant wheat varieties.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Luyu Zhang, Ruiming Zhang, Xinlong Hu, Jing Li, Peiling Zhao, Fei Guo, Hua Zhao, Pu Wang, Yu Wang, Dejiang Ni, Mingle Wang
Summary: This study reports the stably expressed reference genes in tea plants under different nutrient deficiency conditions using three algorithmic programs. The results show that certain genes or combinations of genes have stable expression in the shoots and roots of C. sinensis under different nutrient deficiency conditions. Furthermore, the selected reference genes are validated to accurately quantify gene transcription in tea plants under macro- and medium-element deficient conditions.
SCIENTIA HORTICULTURAE
(2023)
Article
Biology
Dylan Parks, Christian Peterson, Woo-Suk Chang
Summary: Reference genes, also known as housekeeping genes, play a crucial role in gene expression analysis. This study aims to identify and verify reliable reference genes in Bradyrhizobium diazoefficiens, a nitrogen fixing bacterium associated with soybean. The identification of these genes will contribute to the normalization of gene expression data.
Article
Agriculture, Dairy & Animal Science
Muhammed Walugembe, Augustine Naazie, James R. Mushi, George A. Akwoviah, Esther Mollel, Juliana A. Mang'enya, Ying Wang, Nadira Chouicha, Terra Kelly, Peter L. M. Msoffe, Hope R. Otsyina, Rodrigo A. Gallardo, Susan J. Lamont, Amandus P. Muhairwa, Boniface B. Kayang, Huaijun Zhou, Jack C. M. Dekkers
Summary: The study exposed unvaccinated local chicken ecotypes from Ghana and Tanzania to velogenic Newcastle disease virus strains, revealing the genetic basis of ND response traits and suggesting that velogenic ND resistance could be improved through selective breeding of these chicken ecotypes.
Article
Veterinary Sciences
Vinay G. Joshi, Deepika Chaudhary, Nitish Bansal, Renu Singh, Sushila Maan, Nand K. Mahajan, Chintu Ravishankar, Niranjana Sahoo, Sunil K. Mor, Jessica Radzio-Basu, Catherine M. Herzog, Vivek Kapur, Parveen Goel, Naresh Jindal, Sagar M. Goyal
Summary: Newcastle disease virus (NDV) causes Newcastle disease in poultry in India, with a 9.8% apparent prevalence rate found in a surveillance study. The presence of NDV in commercial and backyard poultry emphasizes the importance of surveillance studies even in apparently healthy flocks. The study contributes to a better understanding of NDV epidemiology and could help formulate disease control strategies for both commercial and backyard birds.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Shanyu Cheng, Xinxin Liu, Jiaqi Mu, Weiwen Yan, Mengjun Wang, Haoran Chai, Yuxin Sha, Shanshan Jiang, Sijie Wang, Yongning Ren, Chao Gao, Zhuang Ding, Tobias Stoeger, Erdene-Ochir Tseren-Ochir, Aleksandar Dodovski, Pastor Alfonso, Claro N. Mingala, Renfu Yin
Summary: Highly virulent Newcastle disease virus strains cause severe pathological changes and high mortality, while avirulent strains cause subclinical disease. The host's innate immune responses and metabolic disorders play a role in the differences between these strains.
Article
Chemistry, Applied
Yanchun Hu, Samuel Kumi Okyere, Ruiguang Xu, Guangneng Peng, Zhihua Ren, Junliang Deng, Yan Jia
Summary: This study investigated the antiviral activities of rhein against Newcastle disease virus (NDV) and found that rhein effectively inhibited NDV activities at a maximal safe concentration of 0.125 mg/ml. This suggests rhein could be potentially used as a future antiviral drug against NDV.
NATURAL PRODUCT RESEARCH
(2022)
Article
Biology
Justine Chee, Louise Lanoue, Dave L. Clary, Kendall Higgins, Lynette Bower, Ann Flenniken, Ruolin Guo, David Adams, Fatima Bosch, Robert E. Braun, Steve D. M. Brown, H. -J. Genie Chin, Mary Dickinson, Chih-Wei Hsu, Michael Dobbie, Xiang Gao, Sanjeev Galande, Anne Grobler, Jason Heaney, Yann Herault, Martin Hrabe de Angelis, Fabio Mammano, Lauryl M. J. Nutter, Helen Parkinson, Chuan Qin, Toshi Shiroishi, Radislav Sedlacek, J-K Seong, Ying Xu, Brian Brooks, Colin McKerlie, K. C. Kent Lloyd, Henrik Westerberg, Ala Moshiri
Summary: This study identified new genes and pathways associated with eye development through screening of mouse genes. These findings provide insights into the molecular and cellular mechanisms of eye development and could potentially contribute to the diagnosis and treatment of congenital blinding diseases.
Article
Biochemistry & Molecular Biology
Grace Png, Raffaele Gerlini, Konstantinos Hatzikotoulas, Andrei Barysenka, N. William Rayner, Lucija Klaric, Birgit Rathkolb, Juan A. Aguilar-Pimentel, Jan Rozman, Helmut Fuchs, Valerie Gailus-Durner, Emmanouil Tsafantakis, Maria Karaleftheri, George Dedoussis, Claus Pietrzik, James F. Wilson, Martin Hrabe Angelis, Christoph Becker-Pauly, Arthur Gilly, Eleftheria Zeggini
Summary: In this study, a protein quantitative trait locus (pQTL) analysis was performed on two Greek cohorts, identifying 301 independently associated pQTL variants for 170 proteins related to cardiometabolic processes. The study also discovered rare variants and observed changes in frequency of certain pQTL variants in the isolated populations. Additionally, the study identified proteins causally associated with cardiometabolic traits and described a knock-out Mep1b mouse model.
HUMAN MOLECULAR GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Lillian Garrett, Dietrich Truembach, Nadine Spielmann, Wolfgang Wurst, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe De Angelis, Sabine M. Hoelter
Summary: Neuropsychiatric diseases represent a significant global disease burden and require innovative approaches for pathogenic understanding, biomarker identification, and therapeutic strategies. The malfunction of the heart/brain axis, particularly through the autonomic nervous system and brain central autonomic network interaction, plays a crucial role in the etiology of these diseases. This inter-relationship offers potential avenues for novel diagnosis and treatment approaches.
Article
Biochemistry & Molecular Biology
Nadine Spielmann, Christina Schenkl, Timea Komlodi, Patricia da Silva-Buttkus, Estelle Heyne, Jana Rohde, Oana Amarie, Birgit Rathkolb, Erich Gnaiger, Torsten Doenst, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis, Marten Szibor
Summary: Deletion of the Uqcrh gene causes abnormalities in cardiac morphology and contractility in mice, but does not increase the production of mitochondrial reactive oxygen species. This study provides important information for understanding rare mitochondrial disorders.
Article
Biochemistry & Molecular Biology
Christina Bukas, Isabella Galter, Patricia da Silva-Buttkus, Helmut Fuchs, Holger Maier, Valerie Gailus-Durner, Christian L. Mueller, Martin Hrabe de Angelis, Marie Piraud, Nadine Spielmann
Summary: Echo2Pheno is an automatic statistical learning workflow specifically designed for analyzing and interpreting high-throughput non-anesthetized transthoracic murine echocardiographic images. It accurately confirms known cardiovascular genotype-phenotype relationships and discovers novel genes causing altered cardiovascular phenotypes. This provides an important step toward automatic end-to-end learning for linking echocardiographic readouts to cardiovascular phenotypes in conscious mice.
Article
Biochemistry & Molecular Biology
Patricia da Silva-Buttkus, Nadine Spielmann, Tanja Klein-Rodewald, Christine Schuett, Antonio Aguilar-Pimentel, Oana V. Amarie, Lore Becker, Julia Calzada-Wack, Lillian Garrett, Raffaele Gerlini, Markus Kraiger, Stefanie Leuchtenberger, Manuela A. Oestereicher, Birgit Rathkolb, Adrian Sanz-Moreno, Claudia Stoeger, Sabine M. Hoelter, Claudia Seisenberger, Susan Marschall, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis
Summary: Rare diseases pose challenges in medicine due to their diverse clinical manifestations and low prevalence. The lack of specific treatments for the majority of rare diseases highlights the need for research. Genome sequencing technology allows for the identification of potential disease-causing genes, but further confirmation is required. Mouse knockout models are crucial for studying the genetics of rare diseases and the German Mouse Clinic is a pioneer in this field. Collaboration between research institutions, clinicians, and patient groups is necessary to advance the understanding and treatment of rare diseases.
Article
Chemistry, Multidisciplinary
Furong Tian, Luis Felipe das Chagas e Silva de Carvalho, Alan Casey, Marcelo Saito Nogueira, Hugh J. Byrne
Summary: In recent years, the disease burden of hyperuricemia has been increasing, especially in high-income countries and the economically developing world with a Western lifestyle. Improved methods of uric acid and hypoxanthine detection using surface-enhanced Raman spectroscopy (SERS) and high-performance liquid chromatography (HPLC) were demonstrated by analyzing three different bodily fluids. Gold nanostars were used for quantitative detection of uric acid and hypoxanthine concentrations. The results show strong correlations between HPLC and SERS, and the potential of vibrational spectroscopy in clinical applications was highlighted.
Article
Computer Science, Information Systems
Ziqi Dong, Furong Tian, Hua Yang, Tao Sun, Wenchuan Zhang, Dan Ruan
Summary: Based on heterogeneous trajectories, our proposed framework matches face trajectories with corresponding mobile phone trajectories to achieve object tracking or trajectory prediction. Our solution consists of two stages: selecting phone trajectories for a given face trajectory and identifying which phone trajectory is an exact match. We use a Multi-Granularity SpatioTemporal Window Searching algorithm to select candidate mobile phones close to a given face, and then build an affinity function to score face-phone trajectory pairs and determine if they match. LightGBM achieves the best performance with 92.6% accuracy, 96.9% precision, 88.5% recall, and 92.5% F1. Our framework is applicable in most scenarios and may benefit downstream tasks.
Article
Cell Biology
Daniel Garger, Martin Meinel, Tamina Dietl, Christina Hillig, Natalie Garzorz-Stark, Kilian Eyerich, Martin Hrabe de Angelis, Stefanie Eyerich, Michael P. P. Menden
Summary: Through studying the association between various phenotypic traits and lifespan, it was found that somatic mutation and resting heart rate are negatively correlated with lifespan, while other traits show strong associations. Resting heart rate enhances the prediction of lifespan, indicating its direct influence or representation of lower-level mechanisms associated with lifespan.
Article
Genetics & Heredity
Daniel G. Calame, Tianyu Guo, Chen Wang, Lillian Garrett, Angad Jolly, Moez Dawood, Alina Kurolap, Noa Zunz Henig, Jawid M. Fatih, Isabella Herman, Haowei Du, Tadahiro Mitani, Lore Becker, Birgit Rathkolb, Raffaele Gerlini, Claudia Seisenberger, Susan Marschall, Jill Hunter, Amanda Gerard, Alexis Heidlebaugh, Thomas Challman, Rebecca C. Spillmann, Shalini N. Jhangiani, Zeynep Coban-Akdemir, Seema Lalani, Lingxiao Liu, Anya Revah-Politi, Alejandro Iglesias, Edwin Guzman, Evan Baugh, Nathalie Boddaert, Sophie Rondeau, Clothide Ormieres, Giulia Barcia, Queenie K. G. Tan, Sophie Isabelle Thiffault, Tomi Pastinen, Kazim Sheikh, Suur Biliciler, Davide Mei, Federico Melani, Vandana Shashi, Yuval Yaron, Mary Steele, Emma Wakeling, Elsebet Ostergaard, Francisca Undiagnosed Dis Network, Francisca Millan, Teresa Santiago-Sim, Julien Thevenon, Ange-Line Bruel, Christel Thauvin-Robinet, Denny Popp, Konrad Platzer, Pawel Gawlinski, Wojciech Wiszniewski, Dana Marafi, Davut Pehlivan, Jennifer E. Posey, Richard A. Gibbs, Valerie Gailus-Durner, Renzo Guerrini, Helmut Fuchs, Martin Hrabe de Angelis, Sabine M. Hoelter, Hoi-Hung Cheung, Shen Gu, James R. Lupski
Summary: DExD/H-box RNA helicases (DDX/DHX) are part of a large gene family that encodes enzymes and variations in these genes can lead to neurodevelopmental disorders and cancer. By analyzing genetic data, researchers found rare variants in the DHX9 gene in individuals with different disorders ranging from neurodevelopmental disorders to a specific type of polyneuropathy. Further experiments confirmed that DHX9 is important for neurodevelopment and neuronal homeostasis.
AMERICAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Ecology
Lingjuan Tai, Fen Lu, Lin Huang, Furong Tian, Yun Yang
Summary: This study evaluates the development efficiency of the human-earth system in 8 ethnic autonomous prefectures in Yunnan Province from 1995 to 2017 using a data envelopment analysis (DEA) model, and predicts the development efficiency from 2018 to 2025 using a GA-BP neural network model. The findings show an upward trend in the coordination and coordinated development degree of the human-earth system in Yunnan minority areas, but mainly in low development coordination, and a downward trend in the average comprehensive efficiency value of input and output. The prediction results indicate that most regions will be inefficient in terms of coordinated development by 2025.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Review
Chemistry, Analytical
Thasmin Shahjahan, Bilal Javed, Vinayak Sharma, Furong Tian
Summary: This review evaluates and compares the limits of detection for aflatoxins in recent research articles published between 2015 and 2023. The use of different metal and non-metal nanoparticles has gained interest, with most nanoparticles showing detection limits in the range of 0.01 to 1.0 ng/mL. There is also a significant focus on detecting aflatoxin B1. Overall, the type and size of particles used in lateral-flow immunochromatography assay (LFIA) development can affect sensitivity and detection limits.
Article
Environmental Studies
Gouri Atapattu, Samuel Apori Obeng, Tara Battersby, Michelle Giltrap, Furong Tian
Summary: The microbial ecology of Irish wetlands is not well understood, but it is crucial for the development of effective rewetting schemes to restore and conserve peatlands. This study analyzed microbial populations in peatlands with different land-use types and found significant differences between cutaway and unimproved grassland. The study provides valuable baseline data for further research on bog microbiology and highlights the need to consider microbial interactions with environmental variables in different land-use types.
Article
Endocrinology & Metabolism
Leona Kovac, Thomas Goj, Meriem Ouni, Martin Irmler, Markus Jaehnert, Johannes Beckers, Martin Hrabe De Angelis, Andreas Peter, Anja Moller, Andreas L. Birkenfeld, Cora Weigert, Annette Schuermann
Summary: Exercise training can improve glucose tolerance in obese individuals, but some may not respond positively. This study analyzed gene expression and DNA methylation in skeletal muscle of low and high responders to endurance training, identifying differences in gene expression and potential novel markers for intervention success.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Meeting Abstract
Endocrinology & Metabolism
R. Jumpertz von Schwartzenberg, K. Bodis, J. Martin, M. Schon, M. Hrabe de Angelis, N. Perakakis, S. Kabisch, A. Pfeiffer, M. Blueher, J. Szendroedi, N. Stefan, R. Wagner, A. L. Birkenfeld, M. Roden, A. Fritsche
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
