Article
Biochemistry & Molecular Biology
Chidozie N. Okoye, Shon A. Koren, Andrew P. Wojtovich
Summary: Mitochondria are a significant source of cellular energy, with complex I playing a crucial role in producing ROS that have diverse functions in cell metabolism, serving as important signaling molecules and indicating a mismatch in energy production and demand.
Article
Multidisciplinary Sciences
Blanca Jimenez-Gomez, Patricia Ortega-Saenz, Lin Gao, Patricia Gonzalez-Rodriguez, Paula Garcia-Flores, Navdeep Chandel, Jose Lopez-Barneo
Summary: The hypoxic ventilatory response is triggered by chemoreceptor glomus cells in the carotid body that are connected to the respiratory center in the brainstem. The function of mitochondrial complex I (MCI) in acute oxygen sensing by glomus cells is still unclear. This study shows that transgenic expression of the yeast enzyme NDI1, which does not directly contribute to proton pumping, can fully restore the hypoxic ventilatory response and sensitivity to hypoxia in MCI-deficient mice. These findings highlight the necessity of maintaining mitochondrial NADH dehydrogenase activity for oxygen-dependent regulation of breathing and demonstrate the potential of gene therapy for functional recovery.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Man-Huan Xiao, Yi-Fang Lin, Peng-Peng Xie, Hua-Xing Chen, Jun-Wen Deng, Wei Zhang, Na Zhao, Chen Xie, Yu Meng, Xingguo Liu, Shi-Mei Zhuang, Ying Zhu, Jian-Hong Fang
Summary: This study reveals that downregulation of MPM is associated with increased metastasis potential and recurrence in hepatocellular carcinoma. The restoration of MPM expression inhibits migration and invasion of hepatoma cells, while silencing MPM increases their metastasis ability. MPM interacts with NDUFA7 and regulates mitochondrial complex I activity and the NAD(+)/NADH ratio. miR-17-5p binds to MPM and suppresses its expression.
Article
Biochemistry & Molecular Biology
Eleanor Hay, Louise C. Wilson, Bethan Hoskins, Martin Samuels, Pinki Munot, Shamima Rahman
Summary: This study reports a patient with HIDEA syndrome, characterized by various symptoms including congenital hypotonia, central hypoventilation, intellectual disability, etc., who died at 7 months of age. Biallelic predicted truncating variants in the P4HTM gene were identified, indicating a possible association with the disease. Further investigations suggest that HIDEA syndrome may represent a primary mitochondrial disorder.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Multidisciplinary Sciences
Patricia Gonzalez-Rodriguez, Enrico Zampese, Kristen A. Stout, Jaime N. Guzman, Ema Ilijic, Ben Yang, Tatiana Tkatch, Mihaela A. Stavarache, David L. Wokosin, Lin Gao, Michael G. Kaplitt, Jose Lopez-Barneo, Paul T. Schumacker, D. James Surmeier
Summary: Loss of functional mitochondrial complex I in dopaminergic neurons can lead to a shift in metabolism in mice, causing Parkinson's disease-like symptoms. This study challenges the traditional paradigm of Parkinson's disease by showing that dysfunction of mitochondrial complex I alone is sufficient to cause progressive parkinsonism.
Article
Biotechnology & Applied Microbiology
Ye Sang, Jin-Yu Liu, Feng-Yi Wang, Xiao-Yu Luo, Zi-Qi Chen, Shi-Mei Zhuang, Ying Zhu
Summary: The roles of micropeptides in cell cycle regulation and cancer development remain largely unknown. In this study, the researchers discovered a micropeptide called STMP1 that was up-regulated in various malignancies, including hepatocellular carcinoma (HCC). They found that high levels of STMP1 were associated with shorter recurrence-free survival in HCC patients. Further experiments showed that STMP1 accelerated cell proliferation and tumor growth by enhancing the activity of mitochondrial complex IV, which led to the promotion of key factors in the cell cycle. The findings suggest that STMP1 may serve as a new regulator of the cell cycle and a potential target for anti-cancer therapy.
Article
Multidisciplinary Sciences
Michael Ropke, Daniel Riepl, Patricia Saura, Andrea Di Luca, Max E. Muehlbauer, Alexander Jussupow, Ana P. Gamiz-Hernandez, Ville R. Kaila
Summary: Cellular respiration is powered by redox enzymes that convert chemical energy into an electrochemical proton gradient, driving energy metabolism. Studying conformational changes in mammalian complex I reveals details of proton pumping mechanism. Deactivation of complex I blocks proton transfer, while a potential gating region between membrane domain subunits regulates proton transfer through conformational changes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Aida Rodriguez-Nuevo, Ariadna Torres-Sanchez, Juan M. Duran, Cristian De Guirior, Maria Angeles Martinez-Zamora, Elvan Boke
Summary: Oocytes evade the negative effects of reactive oxygen species (ROS) by eliminating complex I in the mitochondrial electron transport chain. Early oocytes have low levels of complex I and an active mitochondrial unfolded protein response. This suppression of complex I represents a strategy for maintaining biological activity while achieving longevity.
Article
Biochemistry & Molecular Biology
Ivana Kurelac, Beatrice Cavina, Manuela Sollazzo, Stefano Miglietta, Agnese Fornasa, Monica De Luise, Maria Iorio, Eleonora Lama, Daniele Traversa, Hamid Razi Nasiri, Anna Ghelli, Francesco Musiani, Anna Maria Porcelli, Luisa Iommarini, Giuseppe Gasparre
Summary: Inhibition of respiratory complex I (CI) is a promising anti-cancer strategy, but the specifics of its mechanism, efficacy, and specificity are still unclear. By comparing different CI inhibitors on cancer cell models, it was found that BAY 87-2243 and EVP 4593 are selective, while the anti-proliferative effects of metformin are not strongly associated with CI inhibition. This study highlights the caution needed when using metformin as a CI-targeting compound and emphasizes the importance of dosage optimization and evaluation of molecular interactions.
Article
Oncology
Tanaya Lahiri, Lara Brambilla, Joshua Andrade, Manor Askenazi, Beatrix Ueberheide, David E. Levy
Summary: Mitochondrial STAT3 regulates complex I dehydrogenase activity, impacting NAD+ regeneration, thus indirectly controlling antioxidant gene expression to promote proliferation and migration in triple-negative breast cancer cells.
MOLECULAR ONCOLOGY
(2021)
Review
Oncology
Ying Zhang, Hongyi Zhang, Minghong Wang, Thomas Schmid, Zhaochen Xin, Lora Kozhuharova, Wai-Kin Yu, Yuan Huang, Fengfeng Cai, Ewelina Biskup
Summary: In breast cancer, intratumoral hypoxia caused by abnormalities in microvasculature is associated with aggressive phenotype, metastasis risk, and resistance to treatments. Hypoxia-inducible factors (HIFs) play a key role in activating gene transcription related to tumor growth, invasion, and maintenance of cancer stem cell properties. Understanding and targeting hypoxia factors in breast cancer may have significant prognostic and predictive value, potentially impacting treatment outcomes.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Tao Wang, Honghe Liu, Kie Itoh, Sungtaek Oh, Liang Zhao, Daisuke Murata, Hiromi Sesaki, Thomas Hartung, Chan Hyun Na, Jiou Wang
Summary: Haploinsufficiency of C9orf72 is linked to ALS and FTD, with C9orf72 playing a role in regulating cellular energy balance by stabilizing TIMMDC1, a crucial component of OXPHOS complex I in mitochondria.
Article
Chemistry, Multidisciplinary
Gabriele Giachin, Matthew Jessop, Romain Bouverot, Samira Acajjaoui, Melissa Saidi, Anais Chretien, Maria Bacia-Verloop, Luca Signor, Philippe J. Mas, Adrien Favier, Eve Borel Meneroud, Michael Hons, Darren J. Hart, Eaazhisai Kandiah, Elisabetta Boeri Erba, Alain Buisson, Gordon Leonard, Irina Gutsche, Montserrat Soler-Lopez
Summary: ECSIT functions as the bridging node of the MCIA core complex, inducing the transformation of ACAD9 from a FAO enzyme to an MCIA factor, providing a unique molecular mechanism for coordinating the regulation of the FAO and OXPHOS pathways.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Pharmacology & Pharmacy
Eugenia Trushina, Sergey Trushin, Md Fayad Hasan
Summary: Alzheimer's disease, the most common form of dementia in the elderly, has no cure. Emerging data suggest that mitochondria can initiate an integrated stress response that is beneficial for healthy aging and neuroprotection. Partial inhibition of mitochondrial complex I has been explored as a treatment strategy for various diseases, including Alzheimer's disease.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Multidisciplinary Sciences
Enrique Balderas, David R. Eberhardt, Sandra Lee, John M. Pleinis, Salah Sommakia, Anthony M. Balynas, Xue Yin, Mitchell C. Parker, Colin T. Maguire, Scott Cho, Marta W. Szulik, Anna Bakhtina, Ryan D. Bia, Marisa W. Friederich, Timothy M. Locke, Johan L. K. Van Hove, Stavros G. Drakos, Yasemin Sancak, Martin Tristani-Firouzi, Sarah Franklin, Aylin R. Rodan, Dipayan Chaudhuri
Summary: Mitochondrial complex I deficiency is common in congenital, neurologic, and cardiovascular diseases. This study reveals that Complex I stimulates the turnover of a mitochondrial calcium channel, preserving energetic homeostasis during Complex I deficiency. Calcium influx into mitochondria enhances ATP synthesis and is increased in cardiomyopathies caused by mitochondrial dysfunction. The impairment of Complex I leads to increased activity of the mitochondrial calcium uniporter channel. The authors propose that calcium influx compensates for Complex I dysfunction via an impairment in Complex I-induced protein turnover and Uniporter stabilization.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)