Article
Biochemistry & Molecular Biology
Lisa-Marie Wackernagel, Mohsen Abdi Sarabi, Sonke Weinert, Werner Zuschratter, Karin Richter, Klaus Dieter Fischer, Ruediger C. Braun-Dullaeus, Senad Medunjanin
Summary: The NF-κB pathway plays a central role in inflammatory and immune responses, and the activation of NF-κB is dependent on IKK gamma/NEMO. This study reveals that GSK-3 beta phosphorylates NEMO, leading to its localization into multivesicular bodies (MVBs) and endosomes. The AAA-ATPase Vps4A is identified as a key player in NEMO stability and NF-κB activation, interacting directly with phosphorylated NEMO. Manipulation of Vps4A expression results in changes in NEMO expression and NF-κB activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alessandra Pescatore, Ezia Spinosa, Carmela Casale, Maria Brigida Lioi, Matilde Valeria Ursini, Francesca Fusco
Summary: This article reviews the paradigmatic case of Incontinentia Pigmenti (IP), a rare genetic disease caused by NEMO protein deficiency. The loss-of-function mutations of NEMO are embryonically lethal in males, but a few surviving males show the typical clinical presentation observed in females. The pathogenesis of IP may involve the interaction of genetically different cells in the affected tissue and the inflammatory status.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Lingling Li, Wenjuan Liu, Fangshu Li, Baoyu Huang, Qian Liu, Xiaona Wang, Xiuxiu Sang, Juan Dong, Jilv Ma, Jiwen Chen, Lei Wei, Yaqiong Liu, Meiwei Zhang, Yijing Han, Xiaotong Wang
Summary: In this study, the complete coding sequence of the NEMO gene in the scallop Chlamys farreri was obtained and its expression and interaction with the IKK1 protein were studied. The results provide a foundation for understanding the function of invertebrate NEMO and the formation of the IKK complex. The findings have important implications for disease control and management in scallop farming.
Article
Biochemistry & Molecular Biology
Xiangqin Kuang, Zhuojun Zhang, Difeng Li, Wenhao Bao, Jinyuan Pan, Ping Zhou, Han Chen, Zhiqing Gao, Xiaoyi Xie, Chunxiao Yang, Ge Zhu, Zhongqiu Zhou, Ruiming Tang, Zhengfu Feng, Lihuan Zhou, Xiaoli Feng, Lan Wang, Jianan Yang, Lili Jiang
Summary: In bladder cancer, the downregulation of PI16 is associated with disease progression and poor survival. PI16 inhibits tumor growth and metastasis by suppressing the activation of the NF-kappa B pathway through its interaction with ANXA1. These findings suggest that PI16 may serve as both a tumor suppressor and a potential biomarker for metastatic bladder cancer.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
Article
Cell Biology
Hongdian Zhang, Youming Han, Wanyi Xiao, Yongyin Gao, Zhilin Sui, Peng Ren, Fanbiao Meng, Peng Tang, Zhentao Yu
Summary: This study reveals that USP4 plays a crucial role in the progression of esophageal squamous cell carcinoma (ESCC) by modulating proliferation, migration, and invasion. USP4 interacts with TAK1 and stabilizes its protein levels in ESCC cells. Additionally, USP4 promotes ESCC progression through the activation of the MEK/ERK signaling pathway.
CELL DEATH & DISEASE
(2023)
Article
Medicine, Research & Experimental
Kai Song, Xiaofeng Cai, Yunzhou Dong, Hao Wu, Yong Wei, Uma T. Shankavaram, Kui Cui, Yang Lee, Bo Zhu, Sudarshan Bhattacharjee, Beibei Wang, Kun Zhang, Aiyun Wen, Scott Wong, Lili Yu, Lijun Xia, Alana L. Welm, Diane R. Bielenberg, Kevin A. Camphausen, Yibin Kang, Hong Chen
Summary: ER-negative breast cancer exhibits elevated NF-kappa B activity, and here it is shown that epsin proteins play a pivotal role in promoting breast cancer by promoting NEMO linear ubiquitination and sustaining NF-kappa B signaling. The heightened levels of epsins in ER-negative breast cancer are associated with poor relapse-free survival, and targeting epsins may represent a strategy to restrain NF-kappa B signaling and provide a new perspective into ER-negative breast cancer treatment.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Medicine, Research & Experimental
Younglang Lee, Alex W. Wessel, Jiazhi Xu, Julia G. Reinke, Eries Lee, Somin M. Kim, Amy P. Hsu, Jevgenia Zilberman-Rudenko, Sha Cao, Clinton Enos, Stephen R. Brooks, Zuoming Deng, Bin Lin, Adriana A. de Jesus, Daniel N. Hupalo, Daniela G. P. Piotto, Maria T. Terreri, Victoria R. Dimitriades, Clifton L. Dalgard, Steven M. Holland, Raphaela Goldbach-Mansky, Richard M. Siegel, Eric P. Hanson
Summary: This study characterized a pediatric autoinflammatory syndrome called NEMO deleted exon 5 autoinflammatory syndrome (NDAS) in three unrelated male patients. The syndrome is caused by distinct X-linked IKBKG germline mutations that lead to overexpression of a NEMO protein isoform lacking the domain encoded by exon 5 (NEMO-Aex5). The patients exhibited increased NF-KB activation and IFN production in immune cells and blood cells.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Zhenlong Yu, Jian Gao, Xiaolei Zhang, Yulin Peng, Wenlong Wei, Jianrong Xu, Zhenwei Li, Chao Wang, Meirong Zhou, Xiangge Tian, Lei Feng, Xiaokui Huo, Min Liu, Mingliang Ye, De-an Guo, Xiaochi Ma
Summary: In this study, Shikonin (SHK) is identified as a potential inhibitor of the IKK beta/NEMO complex. It disrupts the stability of the complex and significantly impairs the proliferation of colorectal cancer cells.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Mingjian Du, Chee-Kwee Ea, Yan Fang, Zhijian J. Chen
Summary: NEMO acts as a regulatory subunit of the IKK complex and is activated by binding to polyubiquitin chains (polyUb), leading to the formation of liquid-like droplets and activation of IKK and NF-KB signaling.
Article
Virology
Thomas Phelan, Clara Lawler, Andreas Pichlmair, Mark A. A. Little, Andrew G. G. Bowie, Gareth Brady
Summary: Molluscum contagiosum virus (MCV) is a poxvirus that causes a common and persistent infection in humans. It encodes immunosuppressive proteins that inhibit antiviral immunity, particularly through the NF-kappa B signaling pathway. A specific MCV protein, MC008, has been identified to target and inhibit NF-kappa B by disrupting its activation through interaction with NEMO. This finding highlights the evolutionary pressure for MCV to disable key signaling proteins in multiple ways.
JOURNAL OF VIROLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zhu-Qi Huang, Wu Luo, Wei-Xin Li, Pan Chen, Zhe Wang, Rui-Jie Chen, Yi Wang, Wei-Jian Huang, Guang Liang
Summary: Costunolide (CTD) has anti-atherosclerotic effects by reducing inflammatory responses and oxLDL uptake, and its mechanism involves the inhibition of NF-kappa B signaling pathway through covalent binding to IKKβ.
ACTA PHARMACOLOGICA SINICA
(2023)
Letter
Biochemistry & Molecular Biology
Adnan Erol
Summary: Oxidative stress plays a significant role in the development of obesity-related nephropathy (ORN). A causal relationship between IKK and ORN via CYLD-mediated inhibition of NRF2 has been identified. However, contradictory explanations regarding the mechanisms involved need clarification.
MOLECULAR MEDICINE
(2022)
Article
Veterinary Sciences
Yucheng Zhou, Zhaoyang Bu, Jing Qian, Yuening Cheng, Lianjiang Qiao, Sen Yang, Shipeng Cheng, Xinglong Wang, Linzhu Ren, Yanling Yang
Summary: This study revealed that Brucella melitensis UGPase inhibits the activation of NF-kappa B by modulating the ubiquitination of NEMO, providing new insights into the immune mechanisms induced by Brucella.
BMC VETERINARY RESEARCH
(2021)
Article
Immunology
Xiaolong Yan, Xueyan Zhao, Ming Zhou, Yuena Sun, Tianjun Xu
Summary: IRF4b and IRF8 negatively regulate the MITA-mediated NF-kappa B signaling pathway in fish by downregulating MITA expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Neurosciences
Jan Wenzel, Josephine Lampe, Helge Mueller-Fielitz, Raphael Schuster, Marietta Zille, Kristin Mueller, Markus Krohn, Jakob Koerbelin, Linlin Zhang, Umit Ozorhan, Vanessa Neve, Julian U. G. Wagner, Denisa Bojkova, Mariana Shumliakivska, Yun Jiang, Anke Faehnrich, Fabian Ott, Valentin Sencio, Cyril Robil, Susanne Pfefferle, Florent Sauve, Caio Fernando Ferreira Coelho, Jonas Franz, Frauke Spiecker, Beate Lembrich, Sonja Binder, Nina Feller, Peter Koenig, Hauke Busch, Ludovic Collin, Roberto Villasenor, Olaf Joehren, Hermann C. Altmeppen, Manolis Pasparakis, Stefanie Dimmeler, Jindrich Cinatl, Klaus Pueschel, Matija Zelic, Dimitry Ofengeim, Christine Stadelmann, Francois Trottein, Ruben Nogueiras, Rolf Hilgenfeld, Markus Glatzel, Vincent Prevot, Markus Schwaninger
Summary: COVID-19 can cause damage to cerebral small vessels and neurological symptoms by infecting brain endothelial cells and cleaving NEMO through the viral protease M-pro. In animal models, the loss of NEMO leads to the generation of string vessels. Blocking RIPK3, a mediator of regulated cell death, can prevent vessel disruption caused by NEMO ablation, suggesting it as a potential therapeutic target.
NATURE NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)