Article
Biochemistry & Molecular Biology
Hongyu Zhu, Yukiko Kamiya, Hiroyuki Asanuma
Summary: In this study, an imaging system based on fluorescence resonance energy transfer (FRET) was utilized to uncover the interaction mechanism between anti-miR and miRNA in P-bodies, providing valuable insights into the function of anti-miRs and miRNA-targeted therapeutics.
ACS CHEMICAL BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Changrong Wang, Xiaoxia Wang, Lili Du, Yanliang Dong, Bo Hu, Junhui Zhou, Yongli Shi, Suping Bai, Yuanyu Huang, Huiqing Cao, Zicai Liang, Anjie Dong
Summary: The research showed that amphiphilic polycation vehicles with optimal pKa values, strong BCs in the range of pH 5.5-6.5, and low CMCs may be the potential solution for designing efficient vehicles for siRNA delivery. In vitro and in vivo studies supported the effectiveness of EAE5(39/37) in improving siRNA delivery efficiency.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Virology
Carlos Andres Rodriguez-Salazar, Delia Piedad Recalde-Reyes, Juan Pablo Bedoya, Leonardo Padilla-Sanabria, Jhon Carlos Castano-Osorio, Maria Isabel Giraldo
Summary: This study designed and evaluated the combination of siRNA and liposomes to inhibit dengue virus replication. siRNA delivered through liposomes significantly reduced viral titers, without cytotoxicity, hemolysis, or stimulation of pro-inflammatory cytokine release. These results suggest that liposomal delivery of siRNA is a safe and effective method to inhibit dengue virus.
Article
Medicine, Research & Experimental
Ting Pan, Qing Zhou, Kang Miao, Lei Zhang, Guorao Wu, Jun Yu, Yongjian Xu, Weining Xiong, Yong Li, Yi Wang
Summary: Idiopathic pulmonary fibrosis (IPF) is a chronic and diffuse lung disease of unknown etiology with a fatal outcome. Current treatment strategies have not shown significant positive impact on prognosis. Nanomedicine-based gene therapy targeting macrophage polarization may offer a promising strategy for the treatment of pulmonary fibrosis.
Review
Medicine, Research & Experimental
Ida Juhl Corydon, Bjorn Kristensen Fabian-Jessing, Thomas Stax Jakobsen, Asbjorn Cortnum Jorgensen, Emilie Grarup Jensen, Anne Louise Askou, Lars Aagaard, Thomas Juhl Corydon
Summary: The year 2023 is the 25th anniversary of the discovery of RNAi. RNAi-based therapeutics have gained significant momentum in the past decade, with five approved therapeutics targeting the liver and ongoing trials indicating the availability of more RNAi-based medicines for extra-hepatic tissues in the future. This systematic review identified a total of 90 trials published in 81 articles and 48 ongoing trials, investigating the maturation of RNAi-based therapeutics and developments in delivery platforms, administration routes, and potential targets.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Soonkap Kim, Young Gyu Kang, Jaejin Kim, Pooja Dua, Dong-ki Lee
Summary: This study analyzed the gene-silencing ability and immunostimulatory capacity of asiRNAs with different guide strand lengths and found that lasiRNA structures have the potential to function as bifunctional siRNA, triggering a specific immune response combined with target gene silencing.
NUCLEIC ACID THERAPEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Shuai Shi, Anja Madleine Markl, Ziyi Lu, Runhui Liu, Maria Hoernke
Summary: Membrane active compounds can induce various membrane perturbations, including increased membrane permeability, vesicle aggregation or fusion due to colloidal instability, and lipid clustering. In this study, the effects of a partially hydrophobic polycation on leakage and vesicle fusion were investigated. It was found that the polycation had significant activities in both leakage and vesicle fusion. The addition of PEG-lipids could suppress vesicle fusion, but had little effect on leakage activity. These findings have important implications for antimicrobial activity and other areas.
Article
Materials Science, Biomaterials
Xue-Yi Sun, Ya-Xuan Liang, Yi-Nan Gao, Xi Zhang, Rui Liu, Quan Tang, Zhong-Lin Lu, Yang Liu
Summary: A dual-responsive nanoparticle system was developed for the co-delivery of camptothecin and plasmid encoding TNF-related apoptosis-inducing ligand DNA in cancer therapy. The system showed efficient drug release and tumor growth inhibition with minimal side effects.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Shuai Shi, Anja Madleine Markl, Ziyi Lu, Runhui Liu, Maria Hoernke
Summary: Membrane active compounds can induce various types of membrane perturbations, such as membrane permeabilization and vesicle fusion. The partially hydrophobic polycation MM:CO shows strong fusogenic activity with negatively charged vesicles, which can be prevented by PEG-lipids. Besides leakage activity, the fusogenic activity of this polymer may have implications for antimicrobial activity and beyond.
Article
Engineering, Biomedical
Martijn J. W. Evers, Simonides van de Wakker, Ellis M. de Groot, Olivier G. de Jong, Jerney J. J. Gitz-Francois, Cor S. Seinen, Joost P. G. Sluijter, Raymond M. Schiffelers, Pieter Vader
Summary: EV-liposome hybrid nanoparticles have been investigated as a potential siRNA delivery system, demonstrating their ability to encapsulate siRNA, contain EV-surface markers, and functionally deliver siRNA to different cell types. These hybrids combine the benefits of both synthetic and biological drug delivery systems, showing altered functional behavior compared to liposomes and varying efficacy among recipient cell types.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biophysics
Yufeng Zhang, Yanliang Dong, Junhui Zhou, Wenjun Huang, Yidi Wu, Shuyue Zhao, Yongli Shi, Suping Bai, Chunhui Li, Yuanyu Huang, Anjie Dong
Summary: The study reveals the possibility of dual optimization of siRNA intracellular delivery efficiency and antibacterial activity of amphiphilic polycations through rational structure design, making TDDE-3 micelles a promising candidate for potential clinical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Medicine, Research & Experimental
Nisha Chander, Genc Basha, Miffy Hok Yan Cheng, Dominik Witzigmann, Pieter R. Cullis
Summary: By studying LNP mRNA systems with high levels of helper lipids, it was found that LNP mRNA systems containing 40 mol% of DSPC or ESM exhibited significantly improved transfection properties in vitro and extended circulation lifetimes and better tissue accumulation in vivo, especially in terms of gene expression in spleen, bone marrow, and liver.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Chemistry, Physical
Yue Wang, Xiujue Zheng, Jun Liu, Li Chen, Qixian Chen, Yan Zhao
Summary: This article introduces a method for cytoplasmic transportation of therapeutic nucleic acids through mimicking viral vectors. The proposed platform utilizes ligands and pH stimuli to transform the delivery vehicles and transport siRNA into the cytosol. The virus-mimicking platform has demonstrated significant efficacy in anti-tumor therapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Adhika Balgobind, Aliscia Daniels, Mario Ariatti, Moganavelli Singh
Summary: This study assessed the efficacy of cationic lipid-based systems for the delivery of HER2/neu siRNA in breast cancer. The results showed that cationic liposomes efficiently bound, compacted, and protected the therapeutic siRNA against degradation. The non-PEGylated Chol-T-siRNA lipoplexes exhibited the highest HER2/neu silencing.
Article
Multidisciplinary Sciences
Xuexiang Han, Ningqiang Gong, Lulu Xue, Margaret M. Billingsley, Rakan El-Mayta, Sarah J. Shepherd, Mohamad-Gabriel Alameh, Drew Weissman, Michael J. Mitchell
Summary: By synthesizing AA-lipidoids with high delivery efficiency and selectivity, and validating in a preclinical model of liver fibrosis, the potential of AA-lipidoids for targeted RNA delivery to activated fibroblasts has been demonstrated.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)