Review
Biochemistry & Molecular Biology
Dongun Lee, Jeong Hee Hong
Summary: This review discusses the relationship between the ubiquitin-proteasome system (UPS) and calcium (Ca2+) signaling, highlighting their critical roles in different tissue systems and the regulation between them.
Review
Plant Sciences
Dania P. Lobaina, Roberto Tarazi, Tamara Castorino, Maite F. S. Vaslin
Summary: The ubiquitin-proteasome system (UPS) is crucial for maintaining cellular balance and plays a key role in DNA repair, gene transcription, and protein activation. Viruses often manipulate the UPS to maintain viral protein levels and evade host defenses. Understanding the mechanisms used by plant viruses to subvert host defenses is important for developing biotechnological approaches for viral resistance.
Article
Cell Biology
Yanan Li, Shujing Li, Huijian Wu
Summary: Cells adapt to environmental stimuli by making changes to combat injury and increase stress tolerance. If the damage is too severe to repair, cells undergo apoptosis to protect the overall population. Protein aggregation can cause serious damage to the cells, but ubiquitin plays a crucial role in clearing these abnormal proteins. Dysregulation of ubiquitin-proteasome system and autophagy can lead to the development of diseases.
Article
Biochemistry & Molecular Biology
Sarah Poliquin, Jing-Qiong Kang
Summary: Epilepsy is a group of conditions characterized by repeated seizures and commonly coexists with various neurological disorders. Current treatments are unsatisfactory for a significant proportion of patients, highlighting the need for new therapeutic approaches. Disturbed proteostasis, a dynamic balance of protein synthesis and degradation, has emerged as a mechanism in epilepsy that affects neuronal health and function. This review summarizes recent research on the pathomechanisms of abnormal protein folding and degradation in epilepsy and the development of treatments targeting this area.
Article
Biochemistry & Molecular Biology
Zhongchen Ma, Ruirui Li, Ruirui Hu, Wei Zheng, Shuifa Yu, Kejian Cheng, Huan Zhang, Yangyang Xiao, Jihai Yi, Zhen Wang, Yong Wang, Chuangfu Chen
Summary: The study revealed that the effector protein AptA of Anaplasma phagocytophilum interacts with the host cell's proteasome assembly chaperone 3, enhancing proteasome activity and affecting ubiquitination and autophagy mechanisms, while also reducing the apoptotic efficiency of the host cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Hyeongjoo Oh, Min-Kyung Kang, Sin-Hye Park, Dong Yeon Kim, Soo-Il Kim, Su Yeon Oh, Woojin Na, Jae-Hoon Shim, Soon Sung Lim, Young-Hee Kang
Summary: Asaronic acid can inhibit cellular stress caused by 7 beta-hydroxycholesterol, decrease the activation of ER stress sensors, and enhance ubiquitin-proteasomal degradation, potentially serving as a potent atheroprotective agent.
Review
Biochemistry & Molecular Biology
Dina Aweida, Shenhav Cohen
Summary: Protein degradation is essential for cellular integrity, with autophagy and the UPS being the main systems responsible for protein breakdown. Recent studies show that even complex protein structures can be efficiently degraded in vivo, with AAA-ATPases playing a crucial role in the disassembly process.
Article
Cell Biology
Lin Lyu, Zheng Chen, Nami McCarty
Summary: The study reveals TRIM44 as a novel link between the UPS system and autophagy pathway, which enhances aggregate protein clearance rate by promoting SQSTM1/p62 oligomerization and binding of K48 ubiquitin chains.
Article
Biochemistry & Molecular Biology
Utkarsh Reddy Addi, Sneha Jakhotia, S. Sreenivasa Reddy, G. Bhanuprakash Reddy
Summary: Aging is a major risk factor for diseases, including neurodegenerative disorders. This study found that the accumulation of AGEs in the brain increases with age, which may lead to neuronal damage by affecting ER homeostasis, UPS, autophagic flux, and neuronal growth factors.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Biochemistry & Molecular Biology
Peifeng Yu, Zhihua Hua
Summary: The ubiquitin-26S proteasome system and autophagy are two major protein degradation machineries in eukaryotic organisms. They function to maintain proteome homeostasis by degrading short-lived and/or soluble misfolded proteins under normal conditions, and long-lived aggregated proteins and damaged organelles under stress conditions. Recent discoveries have revealed an integrative function of these systems, with ubiquitin playing a central role in their reciprocal degradation. This review summarizes current knowledge about the control of proteasome and autophagosome activity, and presents new research directions for understanding the molecular mechanisms underlying protein degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Karlijne W. Geijtenbeek, Jolien Janzen, Aleksandra E. Bury, Alicia Sanz-Sanz, Ron A. Hoebe, Marie K. Bondulich, Gillian P. Bates, Eric A. J. Reits, Sabine Schipper-Krom
Summary: Huntington's disease, caused by a gene mutation, may be treated by lowering the levels of mutant protein. Research has shown that in affected brain regions, the proteasome separates from PA28αβ, which may be related to disease progression.
Article
Biochemistry & Molecular Biology
Muhammad Saad Khilji, Pouya Faridi, Erika Pinheiro-Machado, Carolin Hoefner, Tina Dahlby, Ritchlynn Aranha, Soren Buus, Morten Nielsen, Justyna Klusek, Thomas Mandrup-Poulsen, Kirti Pandey, Anthony W. Purcell, Michal T. Marzec
Summary: The loss of GRP94 from the endoplasmic reticulum results in mishandling of proinsulin, ER stress, and activation of the immunoproteasome, leading to the sensitization of beta-cells to immune attack.
Article
Cell Biology
Abhishek Singh, Shubhangini Tiwari, Sarika Singh
Summary: The study reveals the significant role of E3 ubiquitin ligase Pirh2 in regulating the aggregation of amyloid-beta protein in Alzheimer's disease. Pirh2 inhibits A beta aggregation by interacting with ER-chaperone GRP78, and improves neuronal morphology and connectivity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Neurosciences
Sandeep Xxxx, Mir Hilal Ahmad, Linchi Rani, Amal Chandra Mondal
Summary: This review summarizes the common shared pathophysiological mechanisms, including insulin resistance, inflammation, mitochondrial dysfunction, endoplasmic reticulum stress, autophagy, and the ubiquitin-proteasome system, that independently mediate the onset and etiopathogenesis of type 2 diabetes mellitus and Parkinson's disease. Understanding these common molecular pathways may provide alternative treatment strategies for both diseases.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ioannis Gkekas, Anna Gioran, Marina Kleopatra Boziki, Nikolaos Grigoriadis, Niki Chondrogianni, Spyros Petrakis
Summary: Neurodegenerative polyglutamine (polyQ) disorders are caused by trinucleotide repeat expansions within disease-causing genes, leading to protein inclusions and oxidative stress. The interplay between oxidative stress, neurodegeneration, and neuroinflammation in polyQ diseases is discussed, with potential pharmaceutical interventions to delay disease progression.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)