Article
Multidisciplinary Sciences
Nontaphat Thongsin, Methichit Wattanapanitch
Summary: Immunotherapy is a promising approach for treating cancer using immune cells such as NK cells. However, the traditional 2D tumor models are limited in their ability to accurately represent the tumor microenvironment. To gain a deeper understanding of immune cell-tumor interactions, more physiologically relevant 3D tumor models have been developed, which can mimic dynamic cellular activities.
Review
Biotechnology & Applied Microbiology
Yaqian Sun, Haiyang Ma
Summary: The drug development process involves evaluating drug activity, analyzing biological indicators, and using them as preclinical drug evaluation criteria. Currently, traditional 2D cell culture is the main method for screening preclinical anticancer drugs. However, this method lacks the ability to simulate the tumor microenvironment and accurately predict drug activity. 3D cell culture, as a technology between 2D cell culture and animal experiments, can better reflect the biological state and predict drug activity and resistance.
BIOTECHNOLOGY LETTERS
(2023)
Article
Oncology
Arturo Temblador, Dimitrios Topalis, Joost van den Oord, Graciela Andrei, Robert Snoeck
Summary: Merkel cell carcinoma (MCC) is a rare and aggressive type of skin cancer for which an in vitro model is still lacking. In this study, the researchers successfully generated a three-dimensional cell culture model of MCC, which can be used to evaluate the efficacy and selectivity of new drug candidates against this disease.
Review
Cell Biology
Anjana K. Badekila, Sudarshan Kini, Amit K. Jaiswal
Summary: 3D cell culture models provide more accurate simulation of in vivo environments, offering insights into various cellular processes. The design of appropriate scaffolds is crucial for organizing the cell microenvironment. The diversification of polymeric scaffolds and advancements in fabrication techniques have significant applications in tissue engineering, drug screening, and tumor model development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Alessandra Flagelli, Olivia Candini, Stella Frabetti, Massimo Dominici, Luciana Giardino, Laura Calza, Vito Antonio Baldassarro
Summary: The study demonstrates that in the novel 3D culture tool VITVO, neural stem/precursor cells spontaneously differentiate into three neural lineages with a higher differentiation rate, and mature oligodendrocytes in VITVO exhibit a myelinating morphology.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Camila L. Campos, Luciana R. Gomes, Ambart E. Covarrubias, Ellen E. Kato, Gisele G. Souza, Silvio A. Vasconcellos, Marcos B. Heinemann, Elizabeth A. L. Martins, Paulo L. Ho, Renata M. A. Da Costa, Josefa B. Da Silva
Summary: Leptospirosis is a global zoonotic disease, and a three-dimensional human lung cell culture model has been established to study the infection mechanism and interaction with the host for this disease.
CURRENT MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hui Niu, Jiarui Xiao, Xiaoli Lou, Lingling Guo, Yongsheng Zhang, Runhuai Yang, Hao Yang, Shouli Wang, Fuzhou Niu
Summary: This study utilized natural silk fibroin and chitosan to fabricate 3D microscaffolds with good biocompatibility for cancer cell culture and drug sensitivity testing. The scaffolds were characterized using various testing methods and showed promising results in facilitating cell growth and drug sensitivity screening.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Oncology
Asmaa F. Khafaga, Shaker A. Mousa, Lotfi Aleya, Mohamed M. Abdel-Daim
Summary: This review focuses on various established 3D culture models of hepatocellular carcinoma (HCC) and their role in investigating tumor-tumor microenvironment interactions and HCC-related therapeutic resistance.
CANCER CELL INTERNATIONAL
(2022)
Article
Nanoscience & Nanotechnology
Jingyuan Wu, Bella Rosa Liyarita, Haishuang Zhu, Ming Liu, Xiao Hu, Fangwei Shao
Summary: DNA hydrogels assembled with dendritic DNA exhibit tunable mechanical strength and reversible thixotropy at low DNA concentrations, enabling high cell proliferation and viability in 3D culture. The bis-entity of dendritic branches allows specific loading of bioactive clues, making it a versatile gel platform for cell regulation.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Chemistry, Multidisciplinary
Shreemoyee De, Neetu Singh
Summary: This review summarizes the major techniques and materials used in developing three-dimensional platforms for cancer research and discusses the challenges and unresolved issues in the field, while highlighting some techniques that have been successfully brought to market.
Review
Chemistry, Multidisciplinary
Tarek Sayde, Omar El Hamoui, Bruno Alies, Karen Gaudin, Gaetane Lespes, Serge Battu
Summary: Three-dimensional cell culture has transformed cellular biology research by allowing for analysis of cellular behavior and responses to microenvironment stimuli. The use of hydrogel scaffold-based models can produce multicellular tumor spheroids when growing cancer cells, providing a platform to study tumor development and toxicity assessment. However, the biggest challenge lies in the reproducibility of the system's components, particularly the multicellular tumor spheroids.
Review
Oncology
Kolluri Poornima, Arul Prakash Francis, Muddasarul Hoda, Mohamed Ahmed Eladl, Srividya Subramanian, Vishnu Priya Veeraraghavan, Mohamed El-Sherbiny, Saad Mohamed Asseri, Abdulrahman Bashir Ahmed Hussamuldin, Krishna Mohan Surapaneni, Ullas Mony, Rukkumani Rajagopalan
Summary: Replicating the natural biomechanical environment of cells is crucial for understanding fundamental life processes. Traditional 2D cell cultures do not replicate the native environment, while 3D cell cultures provide a better replication of extracellular matrix properties and offer insights into tumorigenesis mechanisms. 3D cell culture is a valuable platform for studying the biological characteristics of tumor cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yixuan Lin, Kai Yuan, Yiqi Yang, Shengbing Yang, Kai Huang, Zhifeng Yu, Shuhong Zhang, Yihao Liu, Hanjun Li, Yang Dong, Tingting Tang
Summary: Recent studies have found that the relationship between osteosarcoma and matrix stiffness is often studied in a 2D environment, which may not accurately reflect the impact of matrix stiffness on cell phenotype. By using a 3D bioprinted osteosarcoma model, it was discovered that osteosarcoma cells exhibited different behaviors depending on the stiffness of the matrix. In a softer 3D matrix, the cells proliferated faster, migrated more actively, had a different morphology, and were less sensitive to drugs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Janith Wanigasekara, Lara F. Carroll, Patrick Cullen, Brijesh Tiwari, James Curtin
Summary: Three-dimensional (3D) cell culture models can effectively simulate the natural in vivo environment, shape, tissue stiffness, stressors, gradients, and cellular response, bridging the gap between in vitro cell cultures and in vivo responses, without the associated costs and ethical concerns of animal models.
Article
Biochemistry & Molecular Biology
Enrique Ortega-Forte, Samanta Hernandez-Garcia, Gloria Vigueras, Paula Henarejos-Escudero, Natalia Cutillas, Jose Ruiz, Fernando Gandia-Herrero
Summary: This study reports a novel mitochondria-targeted iridium(III) complex, OncoIr3, which exhibits advantageous properties as a bioimaging agent and shows potent anticancer activity in vitro and in vivo through an oncosis process.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
