期刊
ACS APPLIED MATERIALS & INTERFACES
卷 13, 期 42, 页码 49705-49712出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.1c14445
关键词
DNA hydrogel; 3D cell culture; cell spheroids; dendrimer; self-assembly
资金
- Zhejiang University
- National Natural Science Foundation of China [21975217]
DNA hydrogels assembled with dendritic DNA exhibit tunable mechanical strength and reversible thixotropy at low DNA concentrations, enabling high cell proliferation and viability in 3D culture. The bis-entity of dendritic branches allows specific loading of bioactive clues, making it a versatile gel platform for cell regulation.
With inherent biocompatibility, biodegradability, and unique programmability, hydrogels with a DNA framework show great potential in three-dimensional (3D) cell culture. Here, a DNA hydrogel was assembled by a dendritic DNA with four branches. The hydrogel showed tunable mechanical strength and reversible thixotropy even under a nanomolar DNA concentration. The cell culture medium can be converted into the hydrogel isothermally at physiological temperature. This DNA hydrogel allows both cancer and somatic cells to be seeded in situ and to achieve high proliferation and viability. The bis-entity of dendritic branches enabled the specific loading of bioactive clues to regulate cell behaviors. Thus, the dendritic DNA-assembled hydrogel could serve as a highly biocompatible, readily functionalizing, and easy-casting gel platform for 3D cell culture.
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