Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Article
Horticulture
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou
Summary: The aim of this study was to identify suitable reference genes for normalizing the expression levels of miRNA in different developmental stages and tissues. Five algorithms were used for stability analysis, and the results identified mtr-MIR160b-p3 and gma-miR394a-5p as the most stable miRNAs during bud development in early-flowering tree peony 'Feng dan', while PC-5p-19095 was the most stable during bud development in late-flowering tree peony 'Lian he'. Additionally, mtr-miR159a was found to be the most stable miRNA during flower development stages of different tree peony varieties.
Article
Biochemistry & Molecular Biology
Jin Yang, Zhe Jiang, Qian Xu, Xinyi Liu, Minli Dai, Bing Li, Jing Wei
Summary: This study evaluated the expression stability of nine commonly used reference genes in the dipteran parasitoid, Exorista sorbillans, and identified RP49, eEF1 delta, and 18S rRNA as the most suitable reference genes. This finding provides the necessary foundation for future functional studies in E. sorbillans and its effective use in both sericulture and pest control.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Food Science & Technology
T. -L. Wei, H. Wang, M. -S. Pei, H. -N. Liu, Y. -H. Yu, J. -F. Jiang, D. -L. Guo
Summary: This study aimed to identify suitable reference genes for grapevines, finding that different reference genes should be used in different grapevine samples and identifying a new appropriate reference gene for grapevine (RRM1).
AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH
(2021)
Article
Entomology
Ying-Na Pan, Ru-Na Zhao, Di Fu, Chun Yu, Chun-Ni Pan, Wei Zhou, Wen-Long Chen
Summary: In this study, stable reference genes were screened for Eocanthecona furcellata under different experimental conditions, providing a basis for future molecular studies on this organism.
Article
Fisheries
Baijian Jiang, Qi Li, Zhiqiang Zhang, Yongxiong Huang, Yiqin Wu, Xing Li, Meiling Huang, Yu Huang, Jichang Jian
Summary: This study evaluated the stability of seven common reference genes in Nile tilapia HKLs under different states and found that β-MG was the most suitable reference gene, while GAPDH was the best choice under LPS stimulation. However, there was no single candidate gene that could be used as a universal reference gene in all situations.
AQUACULTURE REPORTS
(2023)
Review
Plant Sciences
Umair Ahmed, Qi Xie, Xueping Shi, Bo Zheng
Summary: This article reviews the importance of developing appropriate reference genes (RGs) for accurately quantifying gene expression levels, and discusses the strategies and processes for developing RGs using different techniques and methods. The most commonly used RGs in horticultural plants and their development strategies are also discussed in detail. The information provided in this article will help in designing effective strategies for developing RGs in horticultural plants.
CRITICAL REVIEWS IN PLANT SCIENCES
(2022)
Article
Agronomy
Junliang Yin, Lu Hou, Xinchen Jiang, Jie Yang, Yang He, Xiaokang Zhou, Xiongmeng Zhu, Andong Gong, Yongxing Zhu, Zhongyi Chen
Summary: Alligatorweed is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance. This study identified stable reference genes for gene expression analysis in A. philoxeroides, laying a foundation for further research on gene expression profiling and invasive weed management.
Article
Genetics & Heredity
Ran-Ran Su, Zhong-Yan Huang, Chao-Wei Qin, Xia-Lin Zheng, Wen Lu, Xiao-Yun Wang
Summary: The study screened and evaluated stable reference genes for Glenea cantor in different adult tissues, developmental stages, and sexes, with RPL32 and EF1A1 identified as the most suitable in different adult tissues, RPL36 and EF1A1 as the best in different developmental stages, and RPL36 and EF1A2 as the optimal for different sexes. GeNorm results indicated that the optimal number of reference genes was two, and the study provided a useful tool for molecular biological studies on G. cantor.
Article
Plant Sciences
Wentao Wang, Xiaohang Zhang, Xiaoxia Xu, Xingchou Xu, Lin Fu, Hongfeng Chen
Summary: This study identified suitable reference genes for qRT-PCR analysis in Ardisia kteniophylla and verified their stability under various experimental conditions. The findings revealed that UBC2 and UBA1 were the most stable reference genes for accurate normalization of target gene expression, while PP2A-2 exhibited the lowest stability. Additionally, the study explored the involvement of certain genes in leaf color change in A. kteniphella. This research provides a foundation for future gene function studies, particularly in the biosynthesis of anthocyanins.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Genetics & Heredity
Hua Cao, Han Li, Lin Lu, Yulu Ji, Lulin Ma, Shenchong Li
Summary: This study selected and evaluated the suitability of ten candidate RGs for normalizing leaf color-related genes' expression levels via qRT-PCR. The stability rankings analysis disclosed that EF1 alpha exhibited the highest stability and was chosen as the most reliable reference gene. The results offer key genetic resources for the functional characterization of leaf color-related genes and will pave the way for molecular dissection of leaf color mutations in D. officinale.
Article
Biochemical Research Methods
Nirmal Kumar Sampathkumar, Venkat Krishnan SundaramID, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aida Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
Summary: The choice of reference genes heavily influences the assessment of differential gene expression by qPCR. Recent studies suggest using RNA-Seq to identify stable genes and statistical approaches to determine the best reference genes. However, this study demonstrates that the statistical approach to determine the best reference genes from commonly used candidates is more important than preselecting stable genes from RNA-Seq data.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Genetics & Heredity
Fuliang Xie, Jianyu Wang, Baohong Zhang
Summary: Identifying suitable reference genes is increasingly important before conducting gene expression analysis. There are several computational tools available for evaluating the stability of candidate reference genes, and RefFinder is the only web-based tool that integrates four different programs for this purpose.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Multidisciplinary Sciences
Saqib Rahman, Zhenzhen Zhao, Muhammad Umair Sial, Yanning Zhang, Hongyun Jiang
Summary: This study confirmed the stability of Actin and 18S as reference genes for normalizing the expression of target genes in Myzus persicae under different conditions. Both genes were effective in normalizing the expression levels of target genes under insecticide treatment and starvation. However, Actin was more stable than 18S at the developmental stages of M. persicae.
Article
Engineering, Electrical & Electronic
Yao Cheng, Zhiwei Wang, Weihua Zhang
Summary: This article proposes a method for rolling bearing fault diagnosis based on a weighted combined square envelope spectrum (WCSES), which decomposes the raw signal into different narrowband signals using the empirical wavelet transform. The WCSES is obtained by weighted averaging the square envelope spectra from different decomposition levels, integrating multiband diagnostic information.
IEEE SENSORS JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Zhiwei Wang, Yao Cheng, Bing Yang, Shoune Xiao, Weihua Zhang
Summary: This article investigates the relationship between raceway defects and vibration signals in the vehicle-track coupled mechanical system. It proposes a dynamic model for HSTABBs and a vehicle-track dynamics model. Rig tests and field experimental tests validate the proposed models, and the vibration defect features of raceway defects are studied. The results show that track irregularity significantly affects the vibration of the axle box, making the defect features unclear, but the skewness in selected statistical indicators is sensitive in detecting raceway local defects.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Electrical & Electronic
Yao Cheng, Ning Zhou, Zhiwei Wang, Bingyan Chen, Weihua Zhang
Summary: A candidate fault frequencies-based blind deconvolution method (CFFsBD) is proposed for enhancing the periodic cyclostationary component caused by bearing defects. This method uses the concept of candidate fault frequencies, which are frequencies most likely to be associated with bearing faults, to guide the solution of the deconvolution filter. The CFFsBD demonstrates robustness and stability in extracting fault-induced cyclostationary symptoms.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Electrical & Electronic
Zongheng Wu, Zhiwei Wang, Teng Liu, Wenzhe Xu, Cai Chen, Yong Kang
Summary: This article proposes a novel partial power regulation topology for data centers, which requires high-efficiency and high-power-density dc-dc converters with wide input range and galvanic isolation. The proposed topology achieves load power delivery through a three-ports LLC-based dc transformer regulated DCX. By introducing an appropriate negative current, the PWM achieves zero voltage switching. Furthermore, ultralow switching frequency can be achieved when the input voltage approaches the lower or upper limit, reducing switching losses and core losses.
IEEE TRANSACTIONS ON POWER ELECTRONICS
(2023)
Article
Computer Science, Hardware & Architecture
Zhenyu Zhang, Guangsen Wang, Qing Liu, Zhiwei Wang, Kang Wang
Summary: This paper proposes a high-precision approximation method for transcendental functions. By dividing the function into two parts based on their slopes, the steep slope part is approximated using an interpolated lookup table, while the gentle slope part is approximated using a range-addressable lookup table algorithm. The boundary between the two parts can be adjusted adaptively according to the desired accuracy. The method achieves arbitrarily low error and consumes fewer hardware resources compared to other methods.
JOURNAL OF CIRCUITS SYSTEMS AND COMPUTERS
(2023)
Article
Engineering, Multidisciplinary
Ning Zhou, Yao Cheng, Zhiwei Wang, Bingyan Chen, Weihua Zhang
Summary: This paper proposes a method called CFFsgram based on candidate fault frequencies (CFFs) for the optimal demodulation frequency band (DFB) identification of axle-box bearings. The vibration signal is divided into different narrowbands using a 1/3-binary tree filter bank constructed by empirical wavelet transform. The local features of the squared envelope spectra of the narrowband signals are used to identify the CFFs, which are frequencies most likely associated with bearing faults. An indicator calculated on the narrowband signals is designed to guide the selection of the DFB. The CFFsgram is shown to be superior in resisting strong noise and random impulses through experiments with challenging datasets.
Article
Mechanics
Kangchun Zhao, Zhixun Xia, Likun Ma, Yifan Duan, Jiarui Zhang, Yunchao Feng, Binbin Cheng, Pengnian Yang
Summary: Large eddy simulation is used to study the gas-particle two-phase transverse jet in supersonic flow. The Eulerian method is used to simulate the fluid, and the Lagrangian method is used to simulate the particles. Different particle mass fractions and Stokes numbers are studied numerically, with the findings that larger particles are mainly located above the counter-rotating vortex pair and smaller particles are mainly located in the counter-rotating vortex pair. Characteristic phenomena such as particle trailing and wave structure constructed of particle cloud are observed, with the particles also lowering the Mach disk.
Article
Engineering, Mechanical
Quan Wang, Zhiwei Wang, Jiliang Mo, Ruichen Wang, Kaiyun Wang
Summary: By using a developed braking system torsional dynamics model, this study investigated the effects of dry and wet rail surface conditions on system dynamic responses. Furthermore, the system stability was explored under different disc-pad friction and wheel-rail adhesion characteristics, revealing the interaction mechanism between the disc-pad and wheel-rail sub-systems. The results showed that the wheel-disc torsional vibration was more complicated under wet rail surface conditions, and the braking system had worse stability when negative slope characteristics of disc-pad friction and wheel-rail adhesion were combined.
TRIBOLOGY INTERNATIONAL
(2023)
Article
Engineering, Aerospace
Yang Li, Zhi-xun Xia, Li-kun Ma, Xu-dong Na
Summary: This study investigated the ignition and extinction characteristics of perchlorate-based electrically controlled solid propellants (ECSPs). The results showed that voltage, pressure, and initial temperature had significant effects on ignition delay and extinction delay. Nonmetallic ECSP exhibited longer ignition delay and better extinguishing performance compared to ECSP containing aluminum. Additionally, four distinct stages of the ECSP ignition process were identified.
JOURNAL OF PROPULSION AND POWER
(2023)
Article
Engineering, Mechanical
Liang Zhang, Zhiwei Wang, Quan Wang, Jiliang Mo, Jian Feng, Kaiyun Wang
Summary: In this study, a rigid-flexible coupled vehicle system dynamics model and a thermo-mechanical coupled finite element (FE) model of a braking system are developed to investigate the effect of wheel polygonal wear (WPW) on temperature and vibration behaviors of a high-speed train braking system. The results show that braking systems with WPW have higher standard deviation (SD) of contact area, root mean square (RMS) values of vibration, and temperature at the friction block interface compared to those without WPW. Additionally, the intensity of vibration in the braking system increases with the increment of WPW order.
MECHANICAL SYSTEMS AND SIGNAL PROCESSING
(2023)
Article
Engineering, Mechanical
Zhiwei Wang, Jiliang Mo, Kaiyun Wang, Zhongrong Zhou
Summary: This study presents a novel vehicle-track coupled dynamics model (VTCDM) that incorporates the disc brake system of a high-speed train (HST). By considering the structure parameters of each friction block, the model accurately calculates the non-linear friction forces and torques, allowing for detailed investigations on the coupled mechanical system and its response to brake sliding friction, wheel-rail rolling friction, and track irregularity. The model is validated through comparisons with experimental results, and it is found that the brake system significantly affects the vehicle-track system (VTS) and exhibits strong non-linear characteristics. Additionally, the model can be employed for tribological and dynamics-related assessments, such as braking squeal and friction-induced vibration.
VEHICLE SYSTEM DYNAMICS
(2023)
Article
Chemistry, Multidisciplinary
Yifan Duan, Pengnian Yang, Zhixun Xia, Yunchao Feng, Chaolong Li, Libei Zhao, Likun Ma
Summary: In this paper, a simple and efficient flow field visualization method based on shadow imaging was applied in a direct-connect test to investigate the influence of the momentum flux ratio and jet angle on the formation and evolution of nitrogen jets in supersonic combustion chambers. The experimental results showed that the method used in this study can clearly capture the formation and evolution of shock wave structures and windward shear vortices. Moreover, the evolution of windward shear vortices is significantly affected by the jet angle, especially when the angle is obtuse.
APPLIED SCIENCES-BASEL
(2023)
Review
Endocrinology & Metabolism
Liyang Jia, Shiqiong Huang, Boyu Sun, Yongguang Shang, Chunsheng Zhu
Summary: Type 2 diabetes mellitus (T2DM) is a major cause of mortality worldwide, often leading to other cardiometabolic risk factors and diabetes-related death. While lifestyle intervention and antidiabetic drugs have been proposed for T2DM management, issues like side effects and drug resistance remain unresolved. The emerging field of pharmacomicrobiomics examines the interplay between the gut microbiome and drug response or toxicity. Increasing evidence supports the gut microbiome as a target for improving drug efficacy and safety through composition manipulation. This review explores the distinct gut microbiome composition in T2DM, its role in disease development, and the potential for personalized treatments utilizing pharmacomicrobiomics.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Transportation Science & Technology
Zhiwei Wang, Linchuan Yang, Jiliang Mo, Song Zhu, Wenwei Jin
Summary: In this paper, a comprehensive trailer car dynamics model (TCDM) considering brake systems is established to reveal the vehicle dynamic behaviour in braking process. The dynamic interactions between the brake system and other connected components are achieved using various factors such as brake disc-pad frictions, brake suspension systems, and wheel-rail interactions. The findings indicate that the braking force significantly affects vehicle dynamic behaviour including the wheel-rail forces, suspension forces, wheelset torsional vibration, etc.
RAILWAY ENGINEERING SCIENCE
(2023)
Article
Engineering, Mechanical
Changlin Lu, Quan Wang, Zhiwei Wang, Jiliang Mo, Song Zhu, Wenwei Jin
Summary: Stick-slip experiments were conducted on a disc-block friction system with different friction radii using a test device. The results analyzed the vibration characteristics and identified the corresponding Stribeck model parameters. The results showed that the vibration amplitude initially increased and then decreased with an increase in the friction radius, while the stick-slip vibration frequency increased. The identified Stribeck model parameters indicated an increase in decay factors, a decrease in static and dynamic friction coefficients with an increase in the friction radius. Furthermore, the exponential form of the Stribeck model accurately reflected the stick-slip characteristics of the disc-block friction system compared to the fractional form. This study has significant implications for the investigation of high-speed train brake systems.
