Review
Physiology
Peng Tan, Lian He, Yun Huang, Yubin Zhou
Summary: Optogenetics combines light and genetics to precisely control living cells, tissues, and organisms. It has advantages such as noninvasiveness, rapid responsiveness, tunability, and high spatiotemporal resolution. This review summarizes the fundamental photochemical properties of optogenetic tools and highlights applications in cellular physiology. It also discusses the progress and future trends in wireless optogenetics.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Endocrinology & Metabolism
Amrita Ahluwalia, Neil Hoa, Debbie Moreira, Daniel Aziz, Karanvir Singh, Khushin N. Patel, Ellis R. Levin
Summary: This study investigates the function of membrane estrogen receptor beta (ER beta) in opposing AngII-induced cardiac cell pathology. The results demonstrate that membrane ER beta plays a crucial role in mitigating cardiac cell pathology and provides insights into the mechanisms of cardiac hypertrophy and fibrosis.
Article
Biology
Alexander S. Hauser, Charlotte Avet, Claire Normand, Arturo Mancini, Asuka Inoue, Michel Bouvier, David E. Gloriam
Summary: Two-thirds of human hormones and one-third of clinical drugs act on membrane receptors coupled to G proteins, but there are differences in the reported G protein couplings among recent large-scale datasets. This study presents a common coupling map that uncovers novel couplings, GPCR-G protein selectivity, and the comparison of co-coupling and co-expression of G proteins with phylogenetic relationships. These findings will advance receptor research and cellular signaling, and contribute to the development of safer drugs.
Article
Peripheral Vascular Disease
Massimo Nardone, Catherine F. Notarius, Mark B. Badrov, Philip J. Millar, John S. Floras
Summary: This study compared the transduction of sympathetic firing into blood pressure in treated patients with heart failure with reduced ejection fraction (HFrEF) and healthy controls. The results showed that treated HFrEF patients had lower sympathetic-BP transduction, even when muscle sympathetic nerve activity (MSNA) was not elevated, and this transduction diminished further with disease progression.
Review
Biochemistry & Molecular Biology
Natsumi Maruta, Yuri Trusov, Alan M. Jones, Jose R. Botella
Summary: Plant G proteins consist of canonical G alpha subunits and unconventional XLG subunits, interacting with G beta gamma dimers and RGS protein, and participating in development, stress responses, and immunity. Despite the overall similarity between plant and animal G alpha subunits, they convey signals differently.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Barkha Ravi, Christine H. Foyer, Girdhar K. Pandey
Summary: Reactive oxygen species (ROS) and calcium (Ca2+) signalling play important roles in plant growth, development, and defense. They function together with electric signals in directional cell-to-cell systemic signalling and even plant-to-plant communication. However, there is still limited understanding of the molecular mechanisms underlying the management of ROS and Ca2+ signals, as well as the synchronous and independent signaling in different cellular compartments. This review focuses on the proteins that may connect different pathways during abiotic stress responses, and highlights the crosstalk between ROS and Ca2+ pathways in cell signaling.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Oncology
Laura van Lieshout, Phyllis van der Ploeg, Yvonne Wesseling-Rozendaal, Anja van de Stolpe, Steven Bosch, Marjolein Lentjes-Beer, Meggy Ottenheijm, Annelen Meriaan, Caroline Vos, Joanne de Hullu, Leon Massuger, Ruud Bekkers, Jurgen Piek
Summary: In postmenopausal patients with advanced stage HGSC, a poorer survival outcome was associated with low functional ER pathway activity, indicating a potential biomarker for predicting survival outcomes. The study highlights the importance of understanding signal transduction pathways in cancer progression and potentially targeting specific pathways for therapeutic interventions.
Article
Pharmacology & Pharmacy
Manal Fardoun, Stefania Mondello, Firas Kobeissy, Ali H. Eid
Summary: Exaggerated cold-induced vasoconstriction can lead to the development of Raynaud's phenomenon. This disease is more prevalent in females and the hormone 17 beta-estradiol may play a role in its etio-pathogenesis. Our research suggests that 17 beta-estradiol stimulates the expression of vascular alpha 2C-adrenoceptors through the G-protein coupled estrogen receptor.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Patrizio Cracco, Emiliano Montalesi, Martina Parente, Manuela Cipolletti, Giovanna Iucci, Chiara Battocchio, Iole Venditti, Marco Fiocchetti, Maria Marino
Summary: A promising therapeutic strategy to delay and/or prevent neurodegenerative diseases could be the restoration of neuroprotective pathways activated by neurons to defend against stress injury. The accumulation of neuroglobin (NGB), induced by the E2/ER beta axis, has been identified as a protective response that enhances mitochondria functionality and prevents apoptosis activation, increasing neuron resilience against oxidative stress. Resveratrol (Res), an ER beta ligand, has shown the ability to reactivate NGB accumulation and exert protective effects against oxidative stress in neuronal-derived cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Michael J. Ziegler, Klaus Yserentant, Valentin Dunsing, Volker Middel, Antoni J. Gralak, Kaisa Pakari, Joern Bargstedt, Christoph Kern, Annett Petrich, Salvatore Chiantia, Uwe Strahle, Dirk-Peter Herten, Richard Wombacher
Summary: The agrochemical mandipropamid (Mandi) has been shown to be a highly efficient chemical inducer of protein proximity (CIP) in cell culture systems and living organisms, with high specificity and orthogonality. It is capable of rapid and efficient protein translocation and network manipulation.
NATURE CHEMICAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ryoiti Kiyama
Summary: Flavonoids are a group of phytoestrogens with physiological effects and ecological and social impacts. Despite the reported estrogenic activity of flavonoids in medical, environmental, and food studies, their molecular mechanisms of action have not been thoroughly reviewed.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Review
Cell Biology
Feng Zeng, Ling Zhu, Qianjin Liao, Xin Li, Yanhong Zhou
Summary: This paper summarizes the subcellular localization of ADPRC in organisms, the related enzyme family members, and the main biological functions of ADPRC. It discusses the relationship between ADPRC and the regulation of calcium signaling, insulin and glucagon secretion, oxytocin secretion, as well as renal and pulmonary vasomotor tension. Furthermore, it expounds the connection between ADPRC and human health and disease occurrence, highlighting its significance in targeted treatment and clinical diagnosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biology
Peter Lonn, Rasel A. Al-Amin, Ehsan Manouchehri Doulabi, Johan Heldin, Radiosa Gallini, Johan Bjorkesten, Johan Oelrich, Masood Kamali-Moghaddam, Ulf Landegren
Summary: The study developed a semi-automated system for large-scale in situ proximity ligation assays (isPLA), expanding the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hannes Schihada, Rawan Shekhani, Gunnar Schulte
Summary: The research team developed and validated eight G protein sensors that can measure the activity of all major families of G proteins, along with a protocol to identify constitutive GPCR or G protein signaling in live cells.
Review
Plant Sciences
Yijie Wang, Jose Ramon Botella
Summary: As sessile organisms, plants have evolved mechanisms to adapt to environmental fluctuations. Heterotrimeric G proteins play essential roles in the response to stress in plants, regulating multiple signaling pathways. Understanding the functions of G proteins in stress reactions can contribute to the development of resilient crops and global food security.
Article
Cell Biology
Ming Zhao, Darren Finlay, Elizabeth Kwong, Robert Liddington, Benoit Viollet, Norio Sasaoka, Kristiina Vuori
Summary: Src and FAK suppress AMPK signaling and autophagy through phosphorylation on a novel site. Autophagy suppression by hydroxychloroquine promotes apoptosis in cancer cells. These findings reveal a link between the Src/FAK complex and AMPK/autophagy regulation.
CELLULAR SIGNALLING
(2022)
Article
Oncology
Yajing Gao, Pekka Paivinen, Sushil Tripathi, Eva Domenech-Moreno, Iris P. L. Wong, Kari Vaahtomeri, Ashwini S. Nagaraj, Sarang S. Talwelkar, Marc Foretz, Emmy W. Verschuren, Benoit Viollet, Yan Yan, Tomi P. Makela
Summary: This study reveals that in LKB1-mutant LUAD, inactivation of AMPK leads to immune evasion, attenuated antigen presentation, and the formation of an immune suppressive tumor microenvironment.
CLINICAL CANCER RESEARCH
(2022)
Editorial Material
Medicine, Research & Experimental
Severine Olivier, Hanna Diounou, Marc Foretz, Sandra Guilmeau, Noemie Daniel, Andre Marette, Malvyne Rolli-Derkinderen, Benoit Viollet
M S-MEDECINE SCIENCES
(2022)
Article
Cell Biology
Adrien Grenier, Laury Poulain, Johanna Mondesir, Arnaud Jacquel, Claudie Bosc, Lucille Stuani, Sarah Mouche, Clement Larrue, Ambrine Sahal, Rudy Birsen, Victoria Ghesquier, Justine Decroocq, Fetta Mazed, Mireille Lambert, Mamy Andrianteranagna, Benoit Viollet, Patrick Auberger, Andrew A. Lane, Pierre Sujobert, Didier Bouscary, Jean-Emmanuel Sarry, Jerome Tamburini
Summary: AMPK activation in AML cells triggers the unfolded protein response, leading to repression of oxidative phosphorylation, TCA cycle, and pyrimidine biosynthesis, as well as enhanced mitochondrial apoptotic signaling. Combination therapy with the AMPK activator GSK621 and the Bcl-2 inhibitor venetoclax shows synergistic effects, suggesting therapeutic potential in AML.
Article
Cell Biology
Valentine Suteau, Mathilde Munier, Rym Ben Boubaker, Meline Wery, Daniel Henrion, Patrice Rodien, Claire Briet
Summary: This study explores the tumoral profiling of G protein-coupled receptors (GPCRs) in endocrine tumors to identify potential targets for drug repurposing. The results show specific GPCR signatures in different types of endocrine tumors, offering new opportunities for drug repurposing.
Article
Neuroimaging
Julien Boucherit, Basile Kerleroux, Gregoire Boulouis, Guillaume Tessier, Christine Rodriguez, Peter B. Sporns, Haroun Ghannouchi, Eimad Shotar, Florent Gariel, Gaultier Marnat, Julien Burel, Heloise Ifergan, Geraud Forestier, Aymeric Rouchaud, Hubert Desal, Anass Nouri, Florent Autrusseau, Gervaise Loirand, Romain Bourcier, Vincent L'Allinec
Summary: This study investigated the changes in geometrical parameters including arterial bifurcation angle, tortuosity, and arterial diameters during the formation and growth of intracranial aneurysms (IA). The results suggest that wider bifurcation angles may be secondary to IA formation at pathologic bifurcation sites.
JOURNAL OF NEUROINTERVENTIONAL SURGERY
(2023)
Article
Nutrition & Dietetics
Tristan Chalvon-Demersay, Claire Gaudichon, Joanna Moro, Patrick C. Even, Nadezda Khodorova, Julien Piedcoq, Benoit Viollet, Julien Averous, Anne-Catherine Maurin, Daniel Tome, Marc Foretz, Pierre Fafournoux, Dalila Azzout-Marniche
Summary: This study investigated the contribution of liver AMPK and GCN2 to the adaptation to high protein intake and found that they were not involved in this adaptation during the postprandial period.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Multidisciplinary Sciences
Nejma Belaadi, Lydia Pernet, Julien Aureille, Gilliane Chadeuf, Marc Rio, Nathalie Vaillant, Elisa Vitiello, Laurence Lafanechere, Gervaise Loirand, Christophe Guilluy
Summary: The rigidity of the extracellular matrix affects cell division, with cells dividing more rapidly on rigid substrates. Changes in matrix stiffness impact mitosis progression, potentially through the regulation of SUN2 expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Endocrinology & Metabolism
Marc Foretz, Bruno Guigas, Benoit Viollet
Summary: This Review presents the latest advances in understanding the mechanisms of action of metformin and discusses its potential repurposing for other indications. Metformin is currently the first-line medication for treating type 2 diabetes in most guidelines and is widely used by over 200 million patients. Its therapeutic action involves complex mechanisms that are not fully understood. Although the liver was initially identified as the main organ involved, increasing evidence suggests the involvement of other sites, including the gastrointestinal tract, gut microbial communities, and tissue-resident immune cells. The molecular mechanisms of metformin vary depending on the dose and duration of treatment, and recent studies have revealed a novel action on the lysosome surface at low concentrations. Considering its efficacy and safety in diabetes, attention has been given to using metformin as adjunct therapy for cancer, age-related diseases, inflammatory diseases, and COVID-19.
NATURE REVIEWS ENDOCRINOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Miroslava Kvandova, Sanela Rajlic, Paul Stamm, Isabella Schmal, Dominika Mihalikova, Marin Kuntic, Maria Teresa Bayo Jimenez, Omar Hahad, Marta Kollarova, Henning Ubbens, Lea Strohm, Katie Frenis, Georg Daniel Duerr, Marc Foretz, Benoit Viollet, Yue Ruan, Subao Jiang, Qi Tang, Hartmut Kleinert, Steffen Rapp, Adrian Gericke, Eberhard Schulz, Matthias Oelze, John F. Keaney, Andreas Daiber, Swenja Kroller-Schon, Thomas Jansen, Thomas Munzel
Summary: This study found that the activation of adenosine monophosphate-activated protein kinase (a1AMPK) through exercise, intermittent fasting, and pharmacological treatment effectively mitigates vascular dysfunction caused by aircraft noise. These findings provide a new approach for the prevention and treatment of transportation noise-associated diseases.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2023)
Article
Biology
Danielle B. Buglak, Pauline Bougaran, Molly R. Kulikauskas, Ziqing Liu, Elizabeth Monaghan-Benson, Ariel L. Gold, Allison P. Marvin, Andrew Burciu, Natalie T. Tanke, Morgan Oatley, Shea N. Ricketts, Karina Kinghorn, Bryan N. Johnson, Celia E. Shiau, Stephen Rogers, Christophe Guilluy, Victoria L. Bautch
Summary: Endothelial cells, which line blood vessels, regulate blood vessel formation and the blood-tissue barrier through cell-cell junctions. The nuclear-localized protein SUN1 is found to be responsible for coordinating vascular sprouting and regulating endothelial cell-cell junctions. Loss of SUN1 impairs blood vessel formation, destabilizes junctions, and results in defective cell-cell connections. SUN1 acts through the LINC complex, microtubules, and Rho-regulated contractility to stabilize junctions and promote their function. This long-range regulation is crucial for proper blood vessel sprouting and maintaining junction integrity.
Article
Biochemistry & Molecular Biology
Julie Favre, Charlotte Roy, Anne-Laure Guihot, Annick Drouin, Manon Laprise, Marc-Antoine Gillis, Simon C. Robson, Eric Thorin, Jean Sevigny, Daniel Henrion, Gilles Kauffenstein
Summary: NTPDase1/CD39 plays a thrombo-immunoregulatory role by controlling endothelial P2 receptor activation. The study highlights the key regulatory function of CD39 in short-term and long-term arterial diameter adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anne Garnier, Jerome Leroy, Claudine Delomenie, Philippe Mateo, Benoit Viollet, Vladimir Veksler, Mathias Mericskay, Renee Ventura-Clapier, Jerome Piquereau
Summary: The study explores the potential control of the beta-adrenergic pathway by AMP-activated protein kinase (AMPK) in the context of compromised energy metabolism. The activation of AMPK was found to down-regulate the expression of adenylyl cyclase 5 (AC5) and blunt the beta-adrenergic cascade, suggesting a compensatory energy sparing mechanism in dysfunctional myocardium.
Article
Allergy
Eleonore Dijoux, Martin Klein, Barbara Misme-Aucouturier, Marie-Aude Cheminant, Marion de Carvalho, Louise Collin, Dorian Hassoun, Erwan Delage, Mathilde Gourdel, Gervaise Loirand, Vincent Sauzeau, Antoine Magnan, Gregory Bouchaud
Summary: Asthma is a chronic inflammatory bronchial disease that affects over 300 million people worldwide, with 70% of cases caused by allergies. This study compared different mouse models of house dust mite-induced allergic asthma and analyzed lung function, barrier integrity, immune response, and microbiota composition. Results showed that nasal and cutaneous sensitization routes led to severe impairment of respiratory function and epithelial dysfunction, while oral sensitization resulted in milder respiratory impairment and mild epithelial dysfunction. Sensitization also led to a significant loss of diversity in lung microbiota.
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
J. Dontaine, L. Bultot, N. Fourny, L. Guilbert, R. Gelinas, F. Mailleux, E. P. Daskalopoulos, H. Esfahani, J-L Balligand, B. Viollet, C. Beauloye, S. Horman, L. Bertrand
EUROPEAN JOURNAL OF HEART FAILURE
(2022)