Article
Critical Care Medicine
Penelope Bourgoin, Ramazan Taspinar, Morgane Gossez, Fabienne Venet, Benjamin Delwarde, Thomas Rimmele, Pierre-Emmanuel Morange, Fabrice Malergue, Guillaume Monneret
Summary: A simplified flow cytometry method for measuring mHLA-DR was developed and showed strong correlation with the routine protocol in septic shock patients. After optimization, this method is suitable for fingerprick blood samples as well.
Article
Immunology
Astrid Fabri, Khalil Kandara, Remy Coudereau, Morgane Gossez, Paul Abraham, Celine Monard, Martin Cour, Thomas Rimmele, Laurent Argaud, Guillaume Monneret, Fabienne Venet
Summary: The study analyzed the impact of IL-10 production in septic patients' whole blood, revealing monocytes and CD4+ T cells as the main IL-10 producers associated with global immunosuppression in septic shock. Further research is needed to determine the pathophysiological mechanisms leading to increased IL-10 production in monocytes and CD4+ T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Remy Coudereau, Louis Waeckel, Martin Cour, Thomas Rimmele, Remi Pescarmona, Astrid Fabri, Laurent Jallades, Hodane Yonis, Morgane Gossez, Anne-Claire Lukaszewicz, Laurent Argaud, Fabienne Venet, Guillaume Monneret
Summary: The study explored the presence of LOX-1+ MDSC in bacterial and viral sepsis, finding significant increases in both patient groups, especially in COVID-19 patients with acute respiratory distress syndrome. The persistence of these cells may contribute to long-lasting immunosuppression in patients.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Review
Immunology
Nicolas Urriola, Stephen Adelstein
Summary: Autoimmune Autonomic Ganglionopathy (AAG) is a rare immune-mediated disease of the autonomic nervous system, and the diagnosis of AAG is challenging due to the complex nature of its multiple components and the interpretation of test results.
AUTOIMMUNITY REVIEWS
(2022)
Article
Medicine, General & Internal
Juan Wu, Sha Yang, Tingting Wang, Qinjuan Wu, Xinyi Liao, Rong Yao, Lei Du
Summary: This study aims to analyze the immune profiles of heatstroke patients, differentiate them from patients with sepsis or aseptic inflammation, and identify diagnostic and prognostic biomarkers. Patients with heatstroke, sepsis, and patients undergoing cardiopulmonary bypass will be recruited for analysis using flow cytometry. The study will also compare gene expression and plasma cytokine levels in specific immune cell populations. The outcomes of the cohorts will be monitored during a 30-day follow-up period.
FRONTIERS IN MEDICINE
(2023)
Review
Pathology
Evdoxia Kyriazopoulou, Evangelos J. Giamarellos-Bourboulis
Summary: This review summarizes the progress in biomarker development for classifying the immune response of septic hosts in the past decade, focusing on immunosuppression and hyperinflammation. It discusses biomarkers such as ferritin, sCD163, and IL-18 for diagnosing complications in sepsis, as well as novel approaches like MDSC and specific endotypes. The future target includes developing specific sepsis endotypes for personalized treatment incorporating biomarkers.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Article
Multidisciplinary Sciences
Nicolas Pavillon, Nicholas I. Smith
Summary: The monitoring of dynamic cellular behaviors is difficult due to the limitations of current techniques for single-cell analysis. In this study, we use label-free optical techniques to non-invasively monitor the changes in murine naive T cells during activation and differentiation. By analyzing spontaneous Raman single-cell spectra, we develop statistical models to detect activation and use non-linear projection methods to track changes during early differentiation. Our label-free results show high correlation with known surface markers and provide spectral models to identify the underlying molecular species involved in the biological process under study.
SCIENTIFIC REPORTS
(2023)
Review
Biochemical Research Methods
Kai Pang, Sihan Dong, Yuxi Zhu, Xi Zhu, Quanyu Zhou, Bobo Gu, Wei Jin, Rui Zhang, Yuting Fu, Bingchen Yu, Da Sun, Duanmu Zheng, Xunbin Wei
Summary: Flow cytometry is a versatile tool for detecting and measuring characteristics of cells or particles. Recent advancements in techniques such as in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC have expanded the applications of FC in basic research and clinical practice.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Infectious Diseases
Nathalie Layios, Christian Gosset, Nathalie Maes, Celine Delierneux, Alexandre Hego, Justine Huart, Christelle Lecut, Pierre Damas, Cecile Oury, Andre Gothot
Summary: The purpose of this study was to identify early changes in leukocyte phenotype that would correlate with later development of sepsis. The results showed that patients who developed secondary sepsis or septic shock had higher monocyte counts, CRP levels, and expression of CD62L(neg) monocytes compared to non-septic patients. In addition, higher SOFA score at admission and lower mHLA-DR levels later on were also associated with sepsis occurrence.
Article
Immunology
Ya Wang, Brian Gloss, Benjamin Tang, Suat Dervish, Brigitte Santner-Nanan, Christina Whitehead, Kristy Masters, Kristen Skarratt, Sally Teoh, Stephen Schibeci, Nicole Fewings, Chrystelle Brignone, Frederic Triebel, David Booth, Anthony McLean, Marek Nalos
Summary: Sepsis is associated with dysregulated inflammatory response and immune suppression, leading to susceptibility to secondary infections. Using high-dimensional flow cytometry, researchers identified two major immune response subgroups in septic patients: hypo-responsive and hyper-responsive. Pre-treatment with IFN-gamma or LAG-3Ig showed that cells from the hypo-responsive group were more responsive to immunostimulants compared to the hyper-responsive group.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Dimitra Gialamprinou, Georgios Mitsiakos, Georgios N. Katsaras, Christos-Georgios Kontovazainitis, Paraskevi Karagianni, Emmanuel Roilides, Gili Kenet
Summary: Neonatal sepsis often leads to coagulopathy, causing severe complications and mortality risk. The interaction between inflammation and coagulation plays a crucial role in neonatal sepsis, but the complexity of this process is not well understood. This review examines the molecular and cellular mechanisms underlying inflammation and hemostasis during neonatal sepsis, taking into account the developmental immune response and hemostasis, with the aim of providing new diagnostic approaches for clinical settings.
Article
Biochemical Research Methods
Jessie E. Arce, Joshua A. Welsh, Sean Cook, John Tigges, Ionita Ghiran, Jennifer C. Jones, Andrew Jackson, Matthew Roth, Aleksandar Milosavljevic
Summary: The NanoFlow Repository, the first implementation of the MIFlowCyt-EV framework, has been developed to meet the need for data sharing and minimum reporting standards for extracellular particles (EPs).
Article
Biochemical Research Methods
Paul H. Dannenberg, Jisoo Kang, Nicola Martino, Anokhi Kashiparekh, Sarah Forward, Jiamin Wu, Andreas C. Liapis, Jie Wang, Seok-Hyun Yun
Summary: This article demonstrates a cell barcode technology using laser particles, which can be used for cell sorting and routing through a microfluidic platform integrated with a high-speed spectrometer. By utilizing the spectral emission of laser particles, individual cells labeled with different laser colors can be distinguished and sorted.
Article
Critical Care Medicine
Tejas Menon Suri, Vijay Hadda, Shadab Ali, Anita Chopra, Maroof Ahmad Khan, Jay Singh, Tamoghna Ghosh, Saurabh Mittal, Pawan Tiwari, Karan Madan, Anant Mohan, Randeep Guleria
Summary: This study explored the associations of leukocyte subpopulations with early clinical deterioration and mortality in sepsis. The results showed that a reduced percentage of CD8 + CD25+ T-cells at enrolment was associated with in-hospital mortality, and a reduced lymphocyte percentage on day 5 was also associated with in-hospital mortality. This suggests that immune dysfunction may be related to the prognosis of sepsis.
JOURNAL OF INTENSIVE CARE MEDICINE
(2023)
Review
Pharmacology & Pharmacy
Chelsea B. Pratt, Matt Alexander
Summary: The development of CAR-T cells is crucial for the success of adoptive T-cell immunotherapy. While the process of producing autologous CAR-T cells is time-consuming and expensive, the exploration of cost-effective allogeneic CAR-T cells is essential. High-throughput tools are used to monitor the entire process of CAR-T cell design, testing, manufacturing, and patient monitoring to ensure safety and efficacy.
DRUG DISCOVERY TODAY
(2022)
Article
Immunology
Tomasz Skirecki, Susanne Drechsler, Grazyna Hoser, Mohammad Jafarmadar, Katarzyna Siennicka, Zygmunt Pojda, Jerzy Kawiak, Marcin F. Osuchowski
FRONTIERS IN IMMUNOLOGY
(2019)
Review
Medicine, Research & Experimental
Jean-Marc Cavaillon, Mervyn Singer, Tomasz Skirecki
EMBO MOLECULAR MEDICINE
(2020)
Review
Critical Care Medicine
Marcin F. Osuchowski, Federico Aletti, Jean-Marc Cavaillon, Stefanie B. Flohe, Evangelos J. Giamarellos-Bourboulis, Markus Huber-Lang, Borna Relja, Tomasz Skirecki, Andrea Szabo, Marc Maegele
Article
Medicine, Research & Experimental
Tomasz Skirecki, Patrycja Swacha, Grazyna Hoser, Jakub Golab, Dominika Nowis, Ewa Kozlowska
Article
Anesthesiology
Anil Gupta, Christian von Heymann, Anders Magnuson, Seppo Alahuhta, Roshan Fernando, Marc Van de Velde, Frederic J. Mercier, Alexandra M. J. Schyns-van den Berg
BRITISH JOURNAL OF ANAESTHESIA
(2020)
Review
Critical Care Medicine
Marcin F. Osuchowski, Martin S. Winkler, Tomasz Skirecki, Sara Cajander, Manu Shankar-Hari, Gunnar Lachmann, Guillaume Monneret, Fabienne Venet, Michael Bauer, Frank M. Brunkhorst, Sebastian Weis, Alberto Garcia-Salido, Matthijs Kox, Jean-Marc Cavaillon, Florian Uhle, Markus A. Weigand, Stefanie B. Flohe, W. Joost Wiersinga, Raquel Almansa, Amanda de la Fuente, Ignacio Martin-Loeches, Christian Meisel, Thibaud Spinetti, Joerg C. Schefold, Catia Cilloniz, Antoni Torres, Evangelos J. Giamarellos-Bourboulis, Ricard Ferrer, Massimo Girardis, Andrea Cossarizza, Mihai G. Netea, Tom van der Poll, Jesus F. Bermejo-Martin, Ignacio Rubio
Summary: The zoonotic SARS-CoV-2 virus causing COVID-19 has devastating consequences worldwide, affecting primarily the respiratory system but also leading to long-term complications. Understanding the pathophysiology and immune response of COVID-19 is crucial for optimizing patient care and treatment strategies.
LANCET RESPIRATORY MEDICINE
(2021)
Review
Medicine, General & Internal
Martin S. Winkler, Tomasz Skirecki, Frank M. Brunkhorst, Sara Cajander, Jean-Marc Cavaillon, Ricard Ferrer, Stefanie B. Flohe, Alberto Garcia-Salido, Evangelos J. Giamarellos-Bourboulis, Massimo Girardis, Matthijs Kox, Gunnar Lachmann, Ignacio Martin-Loeches, Mihai G. Netea, Thibaud Spinetti, Joerg C. Schefold, Antoni Torres, Florian Uhle, Fabienne Venet, Sebastian Weis, Andre Scherag, Ignacio Rubio, Marcin F. Osuchowski
Summary: Animal models have played a crucial role in medical research, especially in studying the novel coronavirus. Apart from clinical studies, these models have provided significant support for COVID-19 drug and vaccine development. During pandemics, optimizing the synergy between preclinical and clinical domains is essential for efficient resource utilization.
Article
Immunology
Tomasz Skirecki, Susanne Drechsler, Aldona Jeznach, Grazyna Hoser, Mohammad Jafarmadar, Jerzy Kawiak, Marcin F. Osuchowski
Summary: The study showed that sepsis impairs the differentiation of stem cells, with a greater impact on P-DIE mice but varying among bone marrow, blood, and spleen compartments. Increased apoptosis, rather than pyroptosis, may contribute to the loss of HSPCs in sepsis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Tomasz Skirecki, Barbara Adamik, Claes Frostell, Urszula Paslawska, Stanislaw Zielinski, Natalia Glatzel-Plucinska, Mateusz Olbromski, Piotr Dziegiel, Waldemar Gozdzik
Summary: This study investigated the effects of iNO and steroid treatment on monocyte subsets in a porcine model of endotoxemia. The results showed decreased SLA-DR expression and increased mitochondrial membrane potential in specific subsets of monocytes. The study also highlighted the importance of studying tissue-resident cells in addition to circulating counterparts.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Iga Stukan, Marek Gryzik, Grazyna Hoser, Andrew Want, Wioleta Grabowska-Pyrzewicz, Mikolaj Zdioruk, Mariola Napiorkowska, Marcin Cieslak, Karolina Krolewska-Golinska, Barbara Nawrot, Grzegorz Basak, Urszula Wojda
Summary: Chemotherapy is widely used for cancer treatment but often leads to therapy resistance. This study discovered that the novel compound BK124.1 can overcome chemotherapy resistance in chronic myeloid leukemia. BK124.1 showed efficacy in animal and cellular models, targeting multidrug resistant leukemia blasts and cancer stem cells. Future development of BK124.1 could offer a cure for chronic myeloid leukemia and other chemotherapy-resistant cancers.
Review
Microbiology
Tomasz Skirecki, Jean-Marc Cavaillon
FEMS MICROBIOLOGY REVIEWS
(2019)
Article
Multidisciplinary Sciences
Tomasz Skirecki, Malgorzata Mikaszewska-Sokolewicz, Marlena Godlewska, Barbara Dolegowska, Jaroslaw Czubak, Grazyna Hoser, Jerzy Kawiak, Urszula Zielinska-Borkowska
SCIENTIFIC REPORTS
(2019)
Meeting Abstract
Critical Care Medicine
Tomasz Skirecki, Patrycja Swacha, Grazyna Hoser, Ewa Kozlowska