Article
Biochemistry & Molecular Biology
Preeti Viswanathan, Yogeshwar Sharma, Fadi-Luc Jaber, Tatyana Tchaikovskaya, Sanjeev Gupta
Summary: The study found that healthy donor hepatocytes can be used as templates for generating specialized cell types to replace metabolic functions and regenerative factors lost in liver failure. By transplanting donor hepatocytes, the ATM and JAK/STAT3 pathways can be restored to advance DNA damage/repair and liver regeneration.
Article
Engineering, Biomedical
Jianyu He, Jinglin Wang, Yuan Pang, Hang Yu, Xueqian Qin, Ke Su, Tao Xu, Haozhen Ren
Summary: The 3D bioprinted hepatic tissue model demonstrated excellent biofunctional and drug-responsive performance, which could potentially be applied in in vitro toxicological studies.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Multidisciplinary Sciences
Barbara Kramar, Dusan Suput, Irina Milisav
Summary: The p16 gene is expressed at low levels in the liver and rat hepatocytes, but varies significantly in hepatocellular carcinoma cell lines and immortalized mouse hepatocytes. These expression differences warrant a closer examination of the associated biochemical pathways in hepatocytes and hepatoma liver models.
Review
Immunology
Jin Gong, Wei Tu, Jingmei Liu, Dean Tian
Summary: Hepatocytes, as the major cells in the liver, have significant roles in maintaining liver homeostasis. They can undergo organelle damage in response to liver insults and inflammatory signals, leading to the release of pro-inflammatory factors and initiation of an inflammatory response. This review summarizes the connection between hepatic organelle damage and liver inflammation, as well as introduces various hepatocyte-derived pro-inflammatory factors in chronic liver injury.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Shuchun Qin, Huijie Lu, Jian Zhang, Xin Ji, Nannan Wang, Jinying Liu, Weili Zhao, Jianhong Wang
Summary: In this study, a reliable fluorescence probe BDP-PN was constructed for the detection and imaging of ONOO-, showing potential applications in drug-induced liver injury and cancer cell diagnosis.
Article
Nutrition & Dietetics
Sin-Hyoung Hong, Yeonhee Hong, Minji Lee, Byeong-Rak Keum, Gun-Hwa Kim
Summary: Skatole is a natural compound derived from plants, insects, and microbial metabolites in the human intestines. It has an anti-lipid peroxidation effect and is a biomarker for several diseases. This study found that skatole could improve hepatocyte lipid metabolism and lipotoxicity induced by hyperlipidemia.
Review
Immunology
Umar Saeed, Zahra Zahid Piracha, Sara Rizwan Uppal, Yasir Waheed, Rizwan Uppal
Summary: During the COVID-19 pandemic, it is important for clinicians to pay attention to SARS-CoV-2-mediated liver injury to manage the increasing burden of hepatocellular carcinoma.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Norihisa Nishimichi, Kazuyuki Tsujino, Keishi Kanno, Kazuhiro Sentani, Tsuyoshi Kobayashi, Kazuaki Chayama, Dean Sheppard, Yasuyuki Yokosaki
Summary: The study identified integrin alpha 8 subunit as the most upregulated in fibroblasts during liver fibrosis, showing relevance to HSC activation, fibroblast differentiation, and TGF beta activation. Experimentally, anti-alpha 8 neutralizing mAb improved pathology and fibrosis markers in murine fibrosis models. The findings suggest integrin alpha 8 beta 1 as a potential target for anti-fibrotic drugs in treating liver fibrosis.
JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ah Ram Lee, Ju-Yeon Cho, Jong Chul Kim, Mehrangiz Dezhbord, Soo Yeun Choo, Chang Hyun Ahn, Na Yeon Kim, Jae Jin Shin, Soree Park, Eun-Sook Park, Juhee Won, Dong-Sik Kim, Jeong-Hoon Lee, Kyun-Hwan Kim
Summary: A study identified multiple viral mutations associated with resistance to tenofovir in a chronic hepatitis B patient who showed suboptimal response after 4 years of treatment. The newly discovered rtT301A mutation significantly impairs HBV replication and reduces sensitivity to tenofovir in hepatoma cell lines, but this effect is restored in primary human hepatocytes. This highlights the importance of validating antiviral drug resistance in different liver cell types to understand viral fitness under treatment pressure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Brandon T. Wesley, Alexander D. B. Ross, Daniele Muraro, Zhichao Miao, Sarah Saxton, Rute A. Tomaz, Carola M. Morell, Katherine Ridley, Ekaterini D. Zacharis, Sandra Petrus-Reurer, Judith Kraiczy, Krishnaa T. Mahbubani, Stephanie Brown, Jose Garcia-Bernardo, Clara Alsinet, Daniel Gaffney, Dave Horsfall, Olivia C. Tysoe, Rachel A. Botting, Emily Stephenson, Dorin-Mirel Popescu, Sonya MacParland, Gary Bader, Ian D. McGilvray, Daniel Ortmann, Fotios Sampaziotis, Kourosh Saeb-Parsy, Muzlifah Haniffa, Kelly R. Stevens, Matthias Zilbauer, Sarah A. Teichmann, Ludovic Vallier
Summary: In this study, the developmental trajectories of human fetal liver cell types were described at single-cell resolution, and bipotential hepatoblast organoids were generated. This provides a platform for investigating human liver development and producing cell types for clinical applications.
NATURE CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Chien Tang, Ya-Wen Chang, Mingtian Che, Mei-Hui Wang, Keith K. Lai, Patrick T. Fueger, Wendong Huang, Shwu-Bin Lin, Keane K. Y. Lai
Summary: This study investigated the potential role of norepinephrine (NE) in thioacetamide-induced liver fibrosis. The results showed that NE could mediate crosstalk between hepatocytes and hepatic stellate cells (HSCs), promoting fibrogenesis. Antagonizing NE or inhibiting its expression could suppress pathological fibrogenesis.
CURRENT MOLECULAR PHARMACOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jiecheng Xu, Daogang Pan, Wei Liao, Zhidong Jia, Mingxin Pan, Jun Weng, Xu Han, Shao Li, Yang Li, Kangyan Liang, Shuqin Zhou, Qing Peng, Yi Gao
Summary: The 3D-P co-culture system demonstrated enhanced liver-specific functions and bile excretion, making it a promising tool for assessing acute/chronic drug hepatotoxicity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Linda Calistri, Vieri Rastrelli, Cosimo Nardi, Davide Maraghelli, Sofia Vidali, Michele Pietragalla, Stefano Colagrande
Summary: The liver plays a crucial role in drug metabolism and detoxification, responding to injury with inflammation, oxidative stress, and necrosis. Advances in chemotherapy have improved patient survival but also increased the incidence of chemotherapy-associated liver injuries. Early recognition of these damages is essential for adjusting treatment regimens to prevent severe complications.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Pharmacology & Pharmacy
Shinichiro Horiuchi, Yukie Kuroda, Yuji Komizu, Seiichi Ishida
Summary: Microphysiological systems (MPS) have been developed to shorten drug development test periods and reduce animal experiments. This study examined different cell sources for liver-MPS, including cryo-preserved hepatocytes, hiPSC-Heps, PXB cells, and HepG2 cells. Gene expression levels and activities were compared, and a focused DNA microarray analysis was conducted. The results provided insights into the characteristics of different hepatocyte cell sources for liver-MPS and contribute to the long-term utilization of an in vitro hepatic assay system.
Article
Pharmacology & Pharmacy
Gundala Venkata Naveen Kumar, Keito Hoshitsuki, Sanjay Rathod, Manda J. Ramsey, Lauren Kokai, Erin E. Kershaw, Wen Xie, Christian A. Fernandez
Summary: PEG-ASNase induced liver injury is characterized by hepatic steatosis, altered gene expression and adipose tissue lipolysis. Obesity and older age are risk factors for exacerbating the injury.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Orthopedics
Elke Maurer, Vera Wallmeier, Marie Reumann, Sabrina Ehnert, Christoph Ihle, Anna J. Schreiner, Ingo Flesch, Laura Emine Stollhof, Tina Histing, Andreas K. Nussler
Summary: The constant aging of the German population leads to increased susceptibility to infections and complications in hospitalised patients, especially in patients with surgical site infections (SSI). Limited studies have been conducted on the age distribution and mortality risk in patients with SSI, as well as the impact of underlying diseases and alcohol and nicotine consumption. This study found that age, pre-existing cardiac conditions, COPD, diabetes mellitus, and peripheral artery disease are significantly associated with an increased mortality risk in patients with SSI. The mortality risk increases exponentially between the ages of 50 and 60, with a 60-year-old patient with SSI having an equivalent mortality risk to an 80-year-old in the general population.
ZEITSCHRIFT FUR ORTHOPADIE UND UNFALLCHIRURGIE
(2023)
Article
Orthopedics
Maximilian M. Menger, Janine Stutz, Sabrina Ehnert, Andreas K. Nussler, Mika F. Rollmann, Steven C. Herath, Benedikt J. Braun, Tim Pohlemann, Michael D. Menger, Tina Histing
Summary: The study introduced a novel ischemic femoral fracture healing model in mice, which showed delayed bone healing. The surgically induced ischemia in the mice resulted in lower Goldberg score, decreased bending stiffness, and reduced bone callus formation, indicating impaired fracture healing process. This model may provide insights into the molecular aspects of ischemic fracture healing and potential new treatment strategies.
Article
Gastroenterology & Hepatology
Sai Wang, Rilu Feng, Shan Shan Wang, Hui Liu, Chen Shao, Yujia Li, Frederik Link, Stefan Munker, Roman Liebe, Christoph Meyer, Elke Burgermeister, Matthias Ebert, Steven Dooley, Huiguo Ding, Honglei Weng
Summary: This study investigates the transcriptional regulation of multidrug resistance protein 2 (MRP2) under different pathophysiological conditions and identifies FOXA2 as a potential replacement for FXR in maintaining MRP2 expression in patients with acute liver failure (ALF). The findings suggest that by regulating the expression of FOXA2, it may be possible to prevent hyperbilirubinemia in ALF patients with sepsis.
Review
Immunology
Jun Tan, Yang Liu, Sabrina Ehnert, Andreas K. Nuessler, Yang Yu, Jianzhong Xu, Tao Chen
Summary: Metagenomic next-generation sequencing (mNGS) displays high accuracy in the diagnosis of prosthetic joint infection (PJI), especially for culture-negative cases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Yoon Seok Jung, Yong-Hoon Kim, Kamalakannan Radhakrishnan, Jung-Ran Noh, Jung Hyeon Choi, Hyo-Jin Kim, Jae-Ho Jeong, Steven Dooley, Chul-Ho Lee, Hueng-Sik Choi
Article
Biotechnology & Applied Microbiology
Maximilian M. Menger, Ruben Manuschewski, Sabrina Ehnert, Mika F. Rollmann, Tanja C. Maisenbacher, Anne L. Tobias, Michael D. Menger, Matthias W. Laschke, Tina Histing
Summary: With a growing elderly population, trauma and reconstructive surgery face challenges in treating geriatric patients. The impact of aging on bone healing remains controversial, prompting an investigation on fracture healing in young adult and aged CD-1 mice. The study revealed delayed callus remodeling and impaired bending stiffness in aged animals, suggesting delayed fracture healing. However, the overall healing capacity of fractured femora was not affected by aging.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Caren Linnemann, Filiz Sahin, Yangmengfan Chen, Karsten Falldorf, Michael Ronniger, Tina Histing, Andreas K. Nussler, Sabrina Ehnert
Summary: This study aimed to evaluate the effect of a 16 Hz extremely low-frequency pulsed electromagnetic field (ELF-PEMF) on neutrophil extracellular trap (NET) formation. The study found that ELF-PEMF exposure did not induce reactive oxygen species and Ca2+-influx in neutrophils, and it reduced the amount of NET release. Therefore, ELF-PEMF exposure can be started immediately after fracture treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Sabrina Ehnert, Helen Rinderknecht, Chao Liu, Melanie Voss, Franziska M. Konrad, Wiebke Eisler, Dorothea Alexander, Kristian-Christos Ngamsri, Tina Histing, Mika F. Rollmann, Andreas K. Nussler
Summary: Chronic wounds have a significant impact on individuals, healthcare systems, and societies worldwide. This study aimed to characterize the differences between acute and chronic wounds regarding angiogenesis, inflammation, and extracellular matrix (ECM) formation and degradation. The results suggest that chronic wounds show increased levels of MMP9 and absence of TGF-beta target gene regulation, indicating the presence of a TGF-beta inhibitor. Increased expression of BAMBI suggests its potential regulatory role in the development of chronic wounds.
Article
Food Science & Technology
Helen Rinderknecht, Alana Mayer, Tina Histing, Sabrina Ehnert, Andreas Nuessler
Summary: Smoking has a significant negative impact on early fracture healing, including inflammation, osteogenesis, and angiogenesis. However, the use of Panax ginseng and maqui berry extract did not have a noticeable positive effect on the early stages of fracture repair in smokers.
Article
Biology
Caren Linnemann, Filiz Sahin, Ningna Li, Stefan Pscherer, Friedrich Goetz, Tina Histing, Andreas K. Nussler, Sabrina Ehnert
Summary: Diabetes is a worldwide disease that often leads to delayed or impaired wound healing. This study investigated the effect of insulin on neutrophil extracellular trap (NET) formation, which is associated with impaired wound healing in diabetic patients. Insulin was found to delay NET formation induced by certain stimuli, but had no effect on another stimulus. Mechanistically, insulin induced reactive oxygen species and phosphorylation of certain proteins, leading to bacterial killing. Insulin may be a potential tool for regulating NET formation in diabetic wound healing.
Article
Biotechnology & Applied Microbiology
Chao Lu, Cosima Prahm, Yangmengfan Chen, Sabrina Ehnert, Helen Rinderknecht, Colin D. McCaig, Andreas K. Nussler, Jonas Kolbenschlag
Summary: Cigarette smoking has negative effects on wound healing, and 100 microampere microcurrent may promote wound healing by enhancing angiogenesis.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Xiaowei Huang, Andreas K. Nussler, Marie K. Reumann, Peter Augat, Maximilian M. Menger, Ahmed Ghallab, Jan G. Hengstler, Tina Histing, Sabrina Ehnert
Summary: The present study established and validated a finite element model (FEM) to predict the mechanical properties of mice tibiae. The FEM showed good correlation with biomechanical data obtained from bending tests. By reducing the need for animal experiments, the FEM can contribute to reducing the number of research animals in bone biomechanics studies.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Helen Rinderknecht, Andreas K. Nussler, Konrad Steinestel, Tina Histing, Sabrina Ehnert
Summary: Angiogenesis plays a crucial role in the early healing process of bone fractures. This study developed an in vitro model to simulate smoking conditions and investigate the impact of smoking on angiogenesis and fracture repair.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Chao Liu, Helen Rinderknecht, Tina Histing, Jonas Kolbenschlag, Andreas K. Nussler, Sabrina Ehnert
Summary: Chronic wounds are a significant problem in clinical work, but there is currently a lack of suitable model systems for studying wound healing. This study successfully established an in vitro scab model that can mimic the interaction between blood cells and skin cells during the early phases of wound healing.
BIOENGINEERING-BASEL
(2022)
Review
Gastroenterology & Hepatology
Yujia Li, Weiguo Fan, Frederik Link, Sai Wang, Steven Dooley
Summary: Transforming growth factor-beta (TGF-beta) is an important factor in the liver that plays a role in various processes initiated by liver injury. It affects different types of liver cells and the response depends on the specific context. In the healthy liver, TGF-beta is stored in the extracellular matrix as latent TGF-beta (L-TGF-beta), and the protein ECM1 is critical for maintaining its latency. Activation of L-TGF-beta has detrimental effects on liver structure and function. This review article presents current knowledge on the formation, secretion, matrix deposition, and activation of L-TGF-beta, and highlights the potential therapeutic benefits of reducing L-TGF-beta activation in liver fibrosis and liver cancer.