Article
Chemistry, Multidisciplinary
Jinxiu Wang, Suze Ma, Wei Ding, Tuo Chen, Qi Zhang
Summary: The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety, and biosynthesis of this moiety involves an oxidoreductase AprQ, which has a unique catalytic mechanism involving a four-electron oxidation in single turnover conditions. AprQ contains a flavin mononucleotide (FMN) cofactor, making it different from other known Q series enzymes, and is classified as a flavoprotein oxidase. This study expands the understanding of the catalytic mechanism of AprQ and the family of flavoprotein oxidases.
CHINESE JOURNAL OF CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Charles Bou-Nader, Frederick W. Stull, Ludovic Pecqueur, Philippe Simon, Vincent Guerineau, Antoine Royant, Marc Fontecave, Murielle Lombard, Bruce A. Palfey, Djemel Hamdane
Summary: The study uncovers that bacterial thymidylate synthase ThyX can utilize a formaldehyde-shunt to directly synthesize thymidylate, providing insights into the mechanism of methylene transfer in folate-dependent reactions.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sana Tanweer, Salma Jamal, Seema Mehra, Najumu Saqib, Faraz Ahmad, Faizan, Abhinav Grover, Sonam Grover
Summary: Tuberculosis is a major cause of death worldwide, with drug-resistant strains posing challenges for clinicians and public health centers. Individuals with compromised immune systems, HIV infection, or diabetes have a high risk of death.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Multidisciplinary Sciences
Horia Duta, Alina Filip, Levente Csaba Nagy, Emma Zsofia Aletta Nagy, Robert Totos, Laszlo Csaba Bencze
Summary: This study reports a toolbox for directed evolution and/or structure-guided protein engineering of FDC, validated on the FDC from Saccharomyces cerevisiae. The developed high-throughput fluorescent plate-assay effectively screened mutant libraries for FDC activity and identified variants with improved activity. Analytical scale biotransformations and computational analysis supported the superior catalytic properties of the identified variants and revealed key residues involved in substrate specificity modulation.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Jinxing Du, Jiaqi Shao, Shengjie Li, Tao Zhu, Hongmei Song, Caixia Lei, Meng Zhang, Yingkun Cen
Summary: Through transcriptome and proteome sequencing, this study identified the important roles of lipid metabolism pathway and steroid biosynthesis pathway in early food habit domestication in Largemouth bass. The results of transcriptome and proteome were reliable.
SCIENTIFIC REPORTS
(2023)
Review
Plant Sciences
Michael P. Torrens-Spence, Christopher M. Glinkerman, Jan Gunther, Jing-Ke Weng
Summary: Imine chemistry plays a crucial role in plant metabolism, involving the biosynthesis of plant natural products and evolution of plant metabolic enzymes. A better understanding of plant imine chemistry will help expand the scope of imine natural product biosynthesis for diverse biotechnological applications.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vipul Panchal, Sushil Kumar, Shaikh Nausad Hossain, Dileep Vasudevan
Summary: This study presents the first thymidylate synthase (TS) structure from a crustacean virus, highlighting its distinction from shrimp and other TS structures, providing important insights for further research.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Nicolas G. Biteau, Vincent Roy, Jean-Christophe Lambry, Hubert F. Becker, Hannu Myllykallio, Luigi A. Agrofoglio
Summary: Researchers discovered a new class of thymidylate synthase for developing new antibacterial agents against multiresistant pathogens. They developed a new class of ANPs based on natural cofactors, with some compounds showing weak inhibitory effects in experiments.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Biology
Luca Costantino, Stefania Ferrari, Matteo Santucci, Outi M. H. Salo-Ahen, Emanuele Carosati, Silvia Franchini, Angela Lauriola, Cecilia Pozzi, Matteo Trande, Gaia Gozzi, Puneet Saxena, Giuseppe Cannazza, Lorena Losi, Daniela Cardinale, Alberto Venturelli, Antonio Quotadamo, Pasquale Linciano, Lorenzo Tagliazucchi, Maria Gaetana Moschella, Remo Guerrini, Salvatore Pacifico, Rosaria Luciani, Filippo Genovese, Stefan Henrich, Silvia Alboni, Nuno Santarem, Anabela da Silva Cordeiro, Elisa Giovannetti, Godefridus J. Peters, Paolo Pinton, Alessandro Rimessi, Gabriele Cruciani, Robert M. Stroud, Rebecca C. Wade, Stefano Mangani, Gaetano Marverti, Domenico D'Arca, Glauco Ponterini, Maria Paola Costi
Summary: A new drug strategy that inhibits the activity of hTS protein and provides a new approach to fight drug-resistant cancers has been discovered.
Review
Chemistry, Medicinal
Sandra Valeria Vassiliades, Lara Gimenez Borges, Jeanine Giarolla, Roberto Parise-Filho
Summary: The folate metabolic cycle is a crucial process for cellular homeostasis and replication control. Recent studies have expanded the potential targets for new antituberculosis drugs by exploring enzymes within this cycle. This review summarizes the history of these enzymes and presents synthetic strategies for the most promising inhibitors.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2023)
Article
Oncology
Anant Ramaswamy, Vikas Ostwal, Atul Sharma, Prabhat Bhargava, Sujay Srinivas, Mahesh Goel, Shraddha Patkar, Sarika Mandavkar, Poonam Jadhav, Manali Parulekar, Amitkumar Choudhari, Sudeep Gupta
Summary: This study found that the use of CAPIRI regimen did not improve overall survival compared to irinotecan alone in patients with advanced gallbladder cancer who had received treatment with a gemcitabine-platinum regimen. More patients in the CAPIRI group required dose modification compared to those in the irinotecan group.
Article
Biochemistry & Molecular Biology
Tyler B. Alt, Matthew R. Hoag, Graham R. Moran
Summary: This article presents the first comprehensive kinetic investigation of a bacterial form of DPD, revealing its similarity to the mammalian form in terms of mechanism but with distinct aspects in substrate roles.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Samantha P. Kelly, Vikram V. Shende, Autumn R. Flynn, Qingyun Dan, Ying Ye, Janet L. Smith, Sachiko Tsukamoto, Matthew S. Sigman, David H. Sherman
Summary: In this study, researchers aimed to develop a unified strategy for biocatalytic construction of prenylated DKP indole alkaloids. By identifying and characterizing a substrate-permissive C2 reverse prenyltransferase (PT) NotF, they found that NotF has a significantly broad substrate scope and strong reaction selectivity. Through the conversion of 30 different structurally differentiated substances, they identified key factors that govern the rate of NotF-catalyzed prenyltransfer and developed an engineering strategy to improve substrate turnover. Finally, they successfully demonstrated the one-pot synthesis of the marine fungal natural product (-)-eurotiumin A using NotF in tandem with BvnB.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Mengting Liu, Masao Ohashi, Yiu-Sun Hung, Kirstin Scherlach, Kenji Watanabe, Christian Hertweck, Yi Tang
Summary: Enzymes capable of halogenation of aliphatic carbons are important to synthetic and biocatalysis fields. AoiQ, a flavin-dependent halogenase, catalyzes gem-dichlorination of 1,3-diketone substrates in the biosynthesis of dichlorodiaporthin, representing the first biochemically reconstituted FDH that can halogenate an enolizable sp(3)-hybridized carbon atom.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Physical
Prabhakar L. Srivastava, Andres M. Escorcia, Florence Huynh, David J. Miller, Rudolf K. Allemann, Marc W. van der Kamp
Summary: Natural sesquiterpene synthases have evolved to quench reactive carbocations in different ways, and this study successfully redesigns the active site of Gd11olS to avoid hydroxylation of the product using a combination of experiment and simulation. Point mutations of selected residues result in variants that predominantly form a nonhydroxylated product.
Article
Biochemistry & Molecular Biology
Dibyendu Mondal, Eric M. Koehn, Jiajun Yao, David F. Wiemer, Amnon Kohen
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Review
Biochemistry & Molecular Biology
Tatiana V. Mishanina, Eric M. Koehn, Amnon Kohen
BIOORGANIC CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Zhen Wang, Anatoly Chernyshev, Eric M. Koehn, Tony D. Manuel, Scott A. Lesley, Amnon Kohen
Article
Chemistry, Multidisciplinary
Tatiana V. Mishanina, Eric M. Koehn, John A. Conrad, Bruce A. Palfey, Scott A. Lesley, Amnon Kohen
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2012)
Article
Multidisciplinary Sciences
Eric M. Koehn, Todd Fleischmann, John A. Conrad, Bruce A. Palfey, Scott A. Lesley, Irimpan I. Mathews, Amnon Kohen
Article
Multidisciplinary Sciences
Eric M. Koehn, Laura L. Perissinotti, Salah Moghram, Arjun Prabhakar, Scott A. Lesley, Irimpan I. Mathews, Amnon Kohen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Chemistry, Multidisciplinary
Eric M. Koehn, John A. Latham, Tara Armand, Robert L. Evans, Xiongying Tu, Carrie M. Wilmot, Anthony T. Iavarone, Judith P. Klinman
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Anatoly Chernyshev, Todd Fleischmann, Eric M. Koehn, Scott A. Lesley, Amnon Kohen
CHEMICAL COMMUNICATIONS
(2007)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)