Article
Chemistry, Applied
Sindija Lapcinska, Pavels Dimitrijevs, Pavel Arsenyan
Summary: A visible light-initiated method has been developed for the preparation of Se-S bond-containing peptides, showing good tolerance, stability, and the ability to serve as an oxidation-sensitive linker.
ADVANCED SYNTHESIS & CATALYSIS
(2021)
Article
Biotechnology & Applied Microbiology
Yingying Wang, Xueman Chen, Qiaoyu Chen, Ning Zhou, Xin Wang, Alei Zhang, Kequan Chen, Pingkai Ouyang
Summary: This study proposes a one-pot method for the production of 5-HT from L-Trp via two enzyme cascades. A chassis cell capable of converting L-Trp to 5-HTP was constructed by expressing tryptophan hydroxylase from Schistosoma mansoni (SmTPH) and an artificial endogenous tetrahydrobiopterin (BH4) module. Then, dopa decarboxylase from Harminia axyridis (HaDDC) was used for the specific catalysis of 5-HTP to 5-HT. The constructed cell factory, E. coli BL21(DE3)Delta tnaA/BH4/HaDDC-SmTPH, achieved the highest 5-HT concentration of 414.5 +/- 1.6 mg/L (conversion rate of 25.9 mol%) under optimal conditions (substrate concentration of 2 g/L, induced temperature of 25 degrees C, IPTG concentration of 0.5 mM, catalysis temperature of 30 degrees C, catalysis time of 72 h).
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Analytical
Isidro Badillo-Ramirez, Bruno Landeros-Rivera, Jose M. Saniger, Juergen Popp, Dana Cialla-May
Summary: The early detection of Parkinson's disease (PD) is important for improving treatment and quality of life. A key metabolite, 5-S-Cysteinyl-dopamine (CDA), can be detected using label-free SERS spectroscopy with a simple and sensitive approach. This study shows the potential of using SERS to detect CDA in aqueous solutions and artificial biofluids, with the ability to identify low concentrations of CDA. This technique can significantly improve the sensitive detection of CDA as a biomarker for further analytical studies and clinical diagnosis in PD patients.
Article
Radiology, Nuclear Medicine & Medical Imaging
Allison S. Cohen, Joe Grudzinski, Gary T. Smith, Todd E. Peterson, Jennifer G. Whisenant, Tiffany L. Hickman, Kristen K. Ciombor, Dana Cardin, Cathy Eng, Laura W. Goff, Satya Das, Robert J. Coffey, Jordan D. Berlin, H. Charles Manning
Summary: PET imaging using C-11-glutamine is safe and effective for noninvasively visualizing metastatic colon cancer lesions in multiple organs, providing potential utility for cancer imaging and monitoring treatment response.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Cell Biology
Marc Morissette, Melanie Bourque, Marie-Eve Tremblay, Therese Di Paolo
Summary: Inflammatory markers were found in the brains of Parkinson's disease patients. The study showed that MPEP reduced the development of L-Dopa-induced dyskinesias (LID) in MPTP-lesioned monkeys and decreased inflammatory markers in the brain. The results demonstrated increased inflammatory markers in the basal ganglia associated with LID and revealed the potential of MPEP in reducing LID and inflammatory response.
Review
Multidisciplinary Sciences
Hidehiko Kumagai, Takane Katayama, Takashi Koyanagi, Hideyuki Suzuki
Summary: This article discusses a simple enzymatic synthesis method using bacterial L-tyrosine phenol-lyase (Tpl) for the production of L-DOPA, a treatment for Parkinson's disease. The research covers screening of bacterial strains, culture conditions, properties and reaction mechanism of the enzyme, as well as the reaction conditions for L-DOPA production. Additionally, the article explores molecular breeding of Tpl-over-producing strains through mutations in the DNA binding protein TyrR.
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES
(2023)
Article
Biology
Ryuji Nishiyama, Takao Ojima, Yuki Ohnishi, Yasuhiro Kumaki, Tomoyasu Aizawa, Akira Inoue
Summary: Nishiyama et al. described a biochemical pathway for converting DEHU to alpha-ketoglutarate in the alginate-assimilating marine bacterium Flavobacterium sp. strain UMI-01. They identified two key enzymes involved in the catabolism of KDGR, revealing the dual pathways of DEHU metabolism involving reduction or oxidation by FlRed.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Applied
Katharina Friedrich, Nicole S. Wermter, Lars Andernach, Katja Witzel, Franziska S. Hanschen
Summary: This study investigated the accumulation and formation of sulfur-containing compounds in Brassica vegetables. The results showed that SMCSO was the main sulfur compound, and it degraded to other compounds upon heating. The SMCSO content was similar in white and red cabbages. Proteome profiling revealed a correlation between the recovery of sulfur compounds and the abundance of two cystine lyase isoforms.
Article
Clinical Neurology
Yulu Song, Tao Gong, Yuanyuan Xiang, Mark Mikkelsen, Guangbin Wang, Richard A. E. Edden
Summary: This preliminary study found that GABA+ levels in the upper brainstem were significantly increased in PD patients post-L-dopa administration, suggesting that dopaminergic therapy may improve GABA+ levels in the upper brainstem and modulate the GABAergic system in the treatment of PD.
JOURNAL OF THE NEUROLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Louay Abo Qoura, Elena Morozova, Vitalia Kulikova, Saida Karshieva, Darina Sokolova, Vasiliy Koval, Svetlana Revtovich, Tatyana Demidkina, Vadim S. Pokrovsky
Summary: The study investigates the anticancer effect of C115H MGL-Dz/propiin on various solid tumor types, both in vitro and in vivo. The results suggest that this pharmacological pair has the potential to suppress tumor growth and can be considered as a new strategy for the treatment of solid tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Betul Teymur, Leo Choubrac, Hannes Hempel, Oki Gunawan, Thomas Unold, David B. Mitzi
Summary: Cu2BaSn(S,Se)4 is a promising environmentally friendly and stable thin-film solar cell material. The copper composition of Cu2BaSn(S,Se)4 was systematically modified and it was found that the material is less sensitive to copper stoichiometry, providing a more stable material platform for thin-film solar cells.
ACS APPLIED ENERGY MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Anthony L. Su, Lawrence H. Lash, Rita Loch-Caruso
Summary: DCVC, a metabolite of TCE, can induce oxidative stress and apoptosis in placental cells. The study found that NAC and AOAA had no significant impact on DCVC toxicity, but CCBL activity and CYP3A4 expression may influence DCVC toxicity. This suggests that the toxicity of DCVC may be related to cellular metabolism.
Article
Neurosciences
Cynthia Kwan, Imane Frouni, Dominique Bedard, Adjia Hamadjida, Stephen G. Nuara, Jim C. Gourdon, Philippe Huot
Summary: Nelotanserin, a serotonin 2A and 2C inverse agonist, was tested for its anti-dyskinetic potential in MPTP-lesioned marmosets. It was found that nelotanserin significantly reduced peak dose dyskinesia and global dyskinesia severity when combined with L-DOPA without affecting its anti-parkinsonian action.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Maike N. Lundahl, Raymond Sarksian, Hao Yang, Richard J. Jodts, Adrien Pagnier, Donald F. Smith, Martin A. Mosquera, Wilfred A. van der Donk, Brian M. Hoffman, William E. Broderick, Joan B. Broderick
Summary: Using freeze-quench techniques and electron paramagnetic resonance (EPR) spectroscopy, the reaction pathway of an adenosylation reaction catalyzed by the radical SAM enzyme is studied. The results provide snapshots of the process, revealing the formation of organic intermediate Omega and the generation of 5'-deoxyadenosyl radical and adenosylated peptide radical. These findings help to understand the mechanism of the reaction.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Feras Altwal, Fernando E. Padovan-Neto, Alexandra Ritger, Heinz Steiner, Anthony R. West
Summary: Research has shown that vilazodone may reduce dyskinesia in patients with Parkinson's disease without interfering with the prokinetic effects of L-DOPA, possibly through its action on 5-HT receptors.
Article
Entomology
Yu Tang, Huaqing Chen, Zhinan Lin, Lei Zhang, Archana Upadhyay, Chenghong Liao, David J. Merkler, Qian Han
Summary: This study provides insight into the molecular evolution of the aaNAT family in mosquitoes. By analyzing genomes of 33 species, it was found that aaNAT orthologs are present in various organisms but absent in certain species. Multiple aaNATs were detected in the Culicinae subfamily of mosquitoes, and their gene expansion occurred more than 190 million years ago through tandem duplication events. Selection analysis revealed that mosquito aaNATs evolved under strong positive pressures, leading to functional diversity.
Article
Genetics & Heredity
Cihan Yang, Chenghong Liao, Jianguo Zhao, Qingfeng Guan, Guoshun Wang, Qian Han
Summary: This study reveals the synergistic impact of alcohol and KYNA on global gene expression, specifically on gene sets related to tryptophan metabolism and cell signaling. While KYNA promotes gene expression and alcohol inhibits it, the co-intake of alcohol and KYNA leads to a stronger inhibition of overall gene expression. Tryptophan metabolism is skewed towards the kynurenine pathway and the GnRH signaling pathway is greatly enhanced. This research provides critical data to better understand the combined effects of alcohol and KYNA on human health.
Editorial Material
Biochemistry & Molecular Biology
Jianyong Li, David J. Merkler, Qian Han
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna L. Kaysheva, Arthur T. Kopylov, Alexander A. Stepanov, Kristina A. Malsagova, Alexander A. Izotov, Yevgeniya I. Shurubor, Boris F. Krasnikov
Summary: A highly sensitive method for the qualitative and quantitative determination of amino- and carboxylic acids, as well as a number of urea and methionine cycle metabolites in the studied solutions, is presented. Derivatives were obtained for amino and carboxylic acids through specific reactions. The developed methods and obtained experimental data are of interest for a wide range of biomedical studies and monitoring the content of endogenous metabolites in biological samples under various pathological conditions.
Article
Nutrition & Dietetics
David Vauzour, Andrew Scholey, David J. White, Neal J. Cohen, Aedin Cassidy, Rachel Gillings, Michael A. Irvine, Colin D. Kay, Min Kim, Rebecca King, Cristina Legido-Quigley, John F. Potter, Hilary Schwarb, Anne-Marie Minihane
Summary: This study investigated the combined effect of omega-3 long-chain polyunsaturated fatty acids (EPA and DHA) and cocoa flavanols on cognitive performance and brain structures in older adults with memory complaints. The results showed that this intervention did not improve cognitive outcomes but led to negative effects, such as increased reaction time variability, decreased alertness and executive function, and a decrease in cortical volume.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Qingfeng Guan, Hui Yang, Chenghong Liao, Jianguo Zhao, Jinhua Wang, Youming Liu, Qian Han, Haiwen Zhang
Summary: In this study, the outer membrane protein YaeT in RA was analyzed, and it was found to be conservative and have sufficient B-cell binding epitopes. Immunization with YaeT protein led to the production of high-affinity antibodies in duck serum, which could activate complement and promote the attack of phagocytes against RA. After RA challenge, the survival rate of YaeT protein-immunized ducks reached 80%.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yevgeniya I. I. Shurubor, Alexander E. E. Rogozhin, Elena P. P. Isakova, Yulia I. I. Deryabina, Boris F. F. Krasnikov
Summary: This study examines the delayed consequences of hepatic encephalopathy (HE) on animal metabolism. The development of acute HE under the influence of thioacetamide (TAA) toxin was found to cause liver pathology, imbalance in CoA and acetyl CoA, and changes in TCA cycle metabolites. Six days after exposure to TAA, alterations in amino acids, related metabolites, glutamine transaminase (GTK) and omega-amidase enzymes were observed in vital organs. Despite apparent physiological recovery, an imbalance in amino acids and enzymes persisted, providing insight into metabolic trends for prognostic and therapeutic purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dejuan Liang, Huaqing Chen, Liping An, Yao Li, Peizhen Zhao, Archana Upadhyay, Bill S. Hansson, Jianguo Zhao, Qian Han
Summary: Ticks are important vectors of pathogens, and understanding their chemoreception mechanisms can help in tick management. Two NPC2 genes were cloned and functionally characterized in Rhipicephalus linnaei, a species of brown dog tick. These genes were expressed in all developmental stages, with the highest expression in adult males. Ligand binding analysis showed that R.linNPC2b had a wide spectrum of ligand binding, while R.linNPC2a had a more narrow range. Molecular docking suggested that specific amino acid residues in these proteins may play a key role in ligand recognition. These findings contribute to our understanding of the olfactory mechanisms in R. linnaei and provide new directions for tick management.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Nutrition & Dietetics
Anneloes Martinsen, Rasha N. M. Saleh, Raphael Chouinard-Watkins, Richard Bazinet, Glenn Harden, James Dick, Noemi Tejera, Matthew G. Pontifex, David Vauzour, Anne-Marie Minihane
Summary: The APOE4 genotype is linked to Alzheimer's disease, while DHA is beneficial to the brain. This study found that cocoa flavanols and APOE genotype can influence the uptake and partitioning of DHA from fish oil, but only in the APOE3 gene.
Article
Entomology
Jing Chen, Yu-Chen Wu, Jiu-Kai Chen, Xiao-Jing Zhu, David Merkler, Cheng-Hong Liao, Qian Han
Summary: In this study, two novel elongase genes were identified in Aedes aegypti, and their functions were investigated using RNAi methods. AeELO2 was found to be involved in larval molting and growth, and it regulated the flexibility and elasticity of adult mosquito cuticles. AeELO9 affected the cold tolerance, osmotic balance, and egg development in Aedes aegypti.
Article
Veterinary Sciences
Yang Lin, Sa Zhou, Archana Upadhyay, Jianguo Zhao, Chenghong Liao, Qingfeng Guan, Jinhua Wang, Qian Han
Summary: This study aimed to understand the prevalence, distribution, and epidemiological profile of Anaplasma spp. infection in dogs in Hainan province/island. Three species of Anaplasma were detected, namely A. phagocytophilum, A. bovis, and A. platys. The overall infection rate was 9.7%, with A. phagocytophilum detected in 1.0% of dogs, A. bovis in 2.7% of dogs, and A. platys in 6.0% of dogs. This surveillance-based study will assist in designing effective control measures and management strategies to treat and control the infection in the area.
VETERINARY SCIENCES
(2023)
Letter
Nutrition & Dietetics
David Vauzour, Anne Marie Minihane
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2023)
Article
Food Science & Technology
Nicole Tosi, Claudia Favari, Letizia Bresciani, Emma Flanagan, Michael Hornberger, Arjan Narbad, Daniele Del Rio, David Vauzour, Pedro Mena
Summary: This study aimed to quantify (Poly)phenol metabolites in plasma and urine following cranberry powder intake in healthy older adults and investigate individual differences by considering urinary metabotypes related to dietary (Poly)phenols. Cranberry consumption led to changes in plasma metabolites and individual variability in urinary metabolites, mainly driven by colonic metabolism. This metabotyping approach is significant for studying the health effects of cranberries and other (Poly)phenol-rich foods, particularly considering gut microbiota-driven individual differences.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Genetics & Heredity
Jitrawadee Intirach, Xin Lv, Qian Han, Zhi-Yue Lv, Tao Chen
Summary: This study identified tick species, genetic diversity, and phylogenetic relationships of ticks in Hainan Island through morphological identification and molecular genetic analysis. Results showed the presence of five tick species on Hainan Island, and phylogenetic analysis based on cox1 and 16S rRNA genes revealed genetic diversity and geographical distribution. The findings also provided evidence of the genetic diversity, host range, and geographical distribution of hard ticks in Hainan Island, China.
Article
Public, Environmental & Occupational Health
Christle Coxon, Piril Hepsomali, Karen Brandt, David Vauzour, Adele Costabile
Summary: In this preliminary study, researchers examined the associations between mental and sleep health and personality traits as well as dietary identity in individuals who follow vegan and vegetarian diets. The findings suggest that both personality traits and dietary identity are related to mental and sleep health in individuals who follow plant-based diets.
HEALTH SCIENCE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)